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recC - exodeoxyribonuclease V subunit RecC (Escherichia coli str. K-12 substr. MG1655)

Gene
Symbol
Dates
  • Create:
    2016-09-14
  • Modify:
    2025-01-20
Description
RecBCD is a bipolar helicase. [More information is available at EcoGene: EG10825]. Exodeoxyribonuclease V gamma chain. [More information is available at EcoCyc: EG10825].

1 Names and Identifiers

1.1 Synonyms

ECK2818

1.2 Other Identifiers

1.2.1 Wikidata

2 Proteins

2.1 Protein Function

A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid (>1 kb/second) and highly processive (>30 kb) ATP-dependent bidirectional helicase. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator, 5'-GCTGGTGG-3') sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to Chi site, by nicking one strand or switching the strand degraded (depending on the reaction conditions). The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang which facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination (PMID: 123277, PMID: 4552016, PMID: 4562392). In the holoenzyme this subunit almost certainly recognizes the wild-type Chi sequence, when added to isolated RecB increases its ATP-dependent helicase processivity. The RecBC complex requires the RecD subunit for nuclease activity, but can translocate along ssDNA in both directions. The RecBCD complex does not unwind G-quadruplex DNA (PMID: 9765292).

2.2 Protein 3D Structures

2.2.1 PDB Structures

2.2.2 NCBI Protein Structures

2.2.3 AlphaFold Structures

Highly accurate protein structure prediction with AlphaFold. Nature. 2021 Aug;596(7873):583-589. DOI:10.1038/s41586-021-03819-2. PMID:34265844; PMCID:PMC8371605

2.3 Protein Targets

3 Chemicals and Bioactivities

3.1 Tested Compounds

4 BioAssays

4.1 Small-Molecule BioAssays

5 Interactions and Pathways

5.1 Interactions

6 Expression

7 Literature

7.1 Consolidated References

7.2 Gene-Chemical Co-Occurrences in Literature

7.3 Gene-Gene Co-Occurrences in Literature

7.4 Gene-Disease Co-Occurrences in Literature

8 Classification

8.1 Gene Ontology: Biological Process

8.2 Gene Ontology: Cellular Component

8.3 Gene Ontology: Molecular Function

9 Information Sources

  1. NCBI Gene
    LICENSE
    NCBI Website and Data Usage Policies and Disclaimers
    https://www.ncbi.nlm.nih.gov/home/about/policies/
  2. PubChem
  3. Database of Interacting Proteins (DIP)
    LICENSE
    All DIP database records available under the terms set by the Creative Commons Attribution-NoDerivs License.
    https://dip.doe-mbi.ucla.edu/dip/termsofuse.html
  4. NCBI Gene Expression Omnibus (GEO)
  5. NCBI Structure
    LICENSE
    NCBI Website and Data Usage Policies and Disclaimers
    https://www.ncbi.nlm.nih.gov/home/about/policies/
  6. RCSB Protein Data Bank (RCSB PDB)
    LICENSE
    Data files contained in the PDB archive (ftp://ftp.wwpdb.org) are free of all copyright restrictions and made fully and freely available for both non-commercial and commercial use. Users of the data should attribute the original authors of that structural data.
    https://www.rcsb.org/pages/policies
  7. UniProt
    LICENSE
    We have chosen to apply the Creative Commons Attribution (CC BY 4.0, http://creativecommons.org/licenses/by/4.0/) License to all copyrightable parts of our databases.
    https://www.uniprot.org/help/license
  8. Wikidata
    exodeoxyribonuclease V subunit RecC b2822
    https://www.wikidata.org/wiki/Q23086692
  9. Gene Ontology (GO)
    LICENSE
    Gene Ontology Consortium data and data products are licensed under the Creative Commons Attribution 4.0 Unported License (https://creativecommons.org/licenses/by/4.0/legalcode)
    http://geneontology.org/docs/go-citation-policy/
  10. AlphaFold DB
    LICENSE
    All of the data provided is freely available for both academic and commercial use under Creative Commons Attribution 4.0 (CC-BY 4.0) licence terms.
    https://alphafold.ebi.ac.uk/faq
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