waaO - UDP-D-glucose:(glucosyl)LPS alpha-1,3-glucosyltransferase (Escherichia coli str. K-12 substr. MG1655)
Gene
Symbol
Dates
- Create:2016-09-14
- Modify:2025-01-12
Description
The lipopolysaccharide of Escherichia coli K-12 consists of two major components: the hydrophobic lipid A moiety inserted into the outer membrane and the phosphorylated core oligosaccharide . [More information is available at EcoCyc: EG11352].
- ECK3617
- rfaI
- waaI
Glucosyltransferase involved in the biosynthesis of the core oligosaccharide region of lipopolysaccharide (LPS) (PMID: 24479701, PMID: 9765561). Catalyzes the addition of a second glucose (glucose II) to the first outer-core glucose (glucose I) (PMID: 24479701, PMID: 9765561). In vitro, can add multiple glucose residues to its lipid acceptor (PMID: 24479701). Activity does not require the branched galactose added by WaaB, but it is higher in the presence of this branched galactose (PMID: 24479701). In the absence of a lipid acceptor, can hydrolyze UDP-glucose, but not UDP-galactose (PMID: 24479701).
Highly accurate protein structure prediction with AlphaFold. Nature. 2021 Aug;596(7873):583-589. DOI:10.1038/s41586-021-03819-2. PMID:34265844; PMCID:PMC8371605
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