Ratio of Kcat to Km for Escherichia coli O55:H7 glycosyltransferase WbgO by Lineweaver-Burke analysis
- Deposit:2010-07-30
- Modify:2022-08-30
Title: Characterization and synthetic application of a novel beta1,3-galactosyltransferase from Escherichia coli O55:H7.
Abstract: A beta1,3-galactosyltransferase (WbgO) was identified in Escherichia coli O55:H7. Its function was confirmed by radioactive activity assay and structure analysis of the disaccharide synthesized with the recombinant enzyme. WbgO requires a divalent metal ion, either Mn(2+) or Mg(2+), for its activity and is active between pH 6.0-8.0 with a pH optimum of 7.0. N-acetylglucosamine (GlcNAc) and oligosaccharides with GlcNAc at the non-reducing end were shown to be its preferred substrates and it can be used for the synthesis of type 1 glycan chains from these substrates. Together with a recombinant bacterial GlcNAc-transferase, benzyl beta-lacto-N-tetraoside was synthesized with the purified WbgO to demonstrate the synthetic utility of WbgO.
Journal: Bioorg Med Chem
Year: 2009
Volume: 17
Issue: 14
First Page: 4910
Last Page: 4915
DOI: 10.1016/j.bmc.2009.06.005
Target ChEMBL ID: CHEMBL3308980
ChEMBL Target Name: Putative glycosyltransferase
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned
- PubChem
- ChEMBLLICENSEAccess to the web interface of ChEMBL is made under the EBI's Terms of Use (http://www.ebi.ac.uk/Information/termsofuse.html). The ChEMBL data is made available on a Creative Commons Attribution-Share Alike 3.0 Unported License (http://creativecommons.org/licenses/by-sa/3.0/).http://www.ebi.ac.uk/Information/termsofuse.html