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Ratio of Kcat to Km for Escherichia coli O55:H7 glycosyltransferase WbgO by Lineweaver-Burke analysis

PubChem AID
450449
Primary Citation
Characterization and synthetic application of a novel beta1,3-galactosyltransferase from Escherichia coli O55:H7 [PMID: 19560364]
Source
External ID
Tested Substances
Tested Compounds
Version
Status
Live
Dates
  • Deposit:
    2010-07-30
  • Modify:
    2022-08-30
Description
This bioassay record (AID 450449) reports results from the above primary citation. Additional data from the same publication are reported in a total of 3 BioAssay records in PubChem.

1 Description

Title: Characterization and synthetic application of a novel beta1,3-galactosyltransferase from Escherichia coli O55:H7.

Abstract: A beta1,3-galactosyltransferase (WbgO) was identified in Escherichia coli O55:H7. Its function was confirmed by radioactive activity assay and structure analysis of the disaccharide synthesized with the recombinant enzyme. WbgO requires a divalent metal ion, either Mn(2+) or Mg(2+), for its activity and is active between pH 6.0-8.0 with a pH optimum of 7.0. N-acetylglucosamine (GlcNAc) and oligosaccharides with GlcNAc at the non-reducing end were shown to be its preferred substrates and it can be used for the synthesis of type 1 glycan chains from these substrates. Together with a recombinant bacterial GlcNAc-transferase, benzyl beta-lacto-N-tetraoside was synthesized with the purified WbgO to demonstrate the synthetic utility of WbgO.

2 Comment

Journal: Bioorg Med Chem

Year: 2009

Volume: 17

Issue: 14

First Page: 4910

Last Page: 4915

DOI: 10.1016/j.bmc.2009.06.005

Target ChEMBL ID: CHEMBL3308980

ChEMBL Target Name: Putative glycosyltransferase

ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain

Relationship Type: D - Direct protein target assigned

Confidence: Direct single protein target assigned

3 Result Definitions

4 Data Table

5 Target

7 Identity

7.1 BioAssay Name

Ratio of Kcat to Km for Escherichia coli O55:H7 glycosyltransferase WbgO by Lineweaver-Burke analysis

7.2 Source

7.3 External ID

7.4 Project Category

Literature, Extracted

7.5 Deposit Date

2010-07-30

7.6 Modify Date

Version 1.1
Version 1.2
Version 1.3
Version 2.1
Version 2.2
Version 2.3
Version 3.1
Version 3.2
Version 3.3
Version 4.1
Version 5.1
Version 5.2
Version 5.3
2022-08-30 (currently shown)

7.7 Status

Live

8 Same-Publication BioAssays

9 BioAssay Annotations

Assay Type
Binding

10 Information Sources

  1. PubChem
  2. ChEMBL
    LICENSE
    Access to the web interface of ChEMBL is made under the EBI's Terms of Use (http://www.ebi.ac.uk/Information/termsofuse.html). The ChEMBL data is made available on a Creative Commons Attribution-Share Alike 3.0 Unported License (http://creativecommons.org/licenses/by-sa/3.0/).
    http://www.ebi.ac.uk/Information/termsofuse.html
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