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Mer Kinase Assay from US Patent US11542259: "Compounds for the treatment of kinase-dependent disorders"

PubChem AID
1919184
Primary Citation
Compounds for the treatment of kinase-dependent disorders [Patent: US11542259]
BioAssay Type
Confirmatory
Tested Substances
Tested Compounds
Version
Status
Live
Dates
  • Deposit:
    2023-10-17
  • Modify:
    2024-11-12
Description
This bioassay record (AID 1919184) reports chemical modulators derived from one or multiple publications as specified in the data table.

1 Description

Human Mer (residues R557-E882 with H628Q and R794A, 0.7 nM) was incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 30 mM NaCl, 250 μM GGMEDIYFEFMGGKKK (SEO ID NO. 44). 10 mM magnesium acetate, and 10 μM [γ-33P-ATP]. The reaction was initiated by the addition of the Mg/ATP mix. After incubation for 40 minutes at room temperature, the reaction was stopped by the addition of phosphoric acid to a concentration of 0.5%. A reaction aliquot of 10 μL was then spotted onto a P30 filtermat and washed four times for 4 minutes in 0.425% phosphoric acid and once in methanol prior to drying and scintillation counting. Incorporated 33P was measured using the Wallac Microbeta scintillation counter (Perkin Elmer).

2 Comment

Compounds with any of Ki, IC50, Kd, or EC50 activity value <= 10uM are labeled as "Active".

If multiple measurements are available for a given compound, it is labeled as "Active" if any of the measurements meet the criterion. Activity values are checked in the order of Ki, IC50, Kd, and EC50. The first entry that meets the above activity threshold is used to determine "Standard Type", "Standard Relation", and "PubChem Standard Value". Otherwise, the first non-empty entry will be used to set those values.

3 Result Definitions

4 Data Table

5 Target

7 Cross-References

Patent XRefs

9 Identity

9.1 BioAssay Name

Mer Kinase Assay from US Patent US11542259: "Compounds for the treatment of kinase-dependent disorders"

9.2 Source

9.3 External ID

9.4 Project Category

Literature, Extracted

9.5 BioAssay Type

Confirmatory

9.6 Deposit Date

2023-10-17

9.7 Modify Date

Version 1.1
Version 2.1
2024-11-12 (currently shown)

9.8 Status

Live

10 Same-Publication BioAssays

11 Information Sources

  1. PubChem
  2. BindingDB
    LICENSE
    All data curated by BindingDB staff are provided under the Creative Commons Attribution 3.0 License (https://creativecommons.org/licenses/by/3.0/us/).
    https://www.bindingdb.org/rwd/bind/info.jsp
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