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BioAssay: AID 725227

Activation of Sulfurihydrogenibium yellowstonense recombinant CA by stopped flow CO2 hydrase method

The alpha-carbonic anhydrase (CA, EC 4.2.1.1) from the extremophilic bacterium Sulfurihydrogenibium azorense (SazCA) was recently shown to be the fastest CA known. Here we investigated this enzyme for its activation with a series of amino acids and amines. The best SazCA activators were D-Phe, L-DOPA, L- and D-Trp, dopamine and serotonin, which showed activation constants in the range of 3-23 nM. more ..
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 Tested Compounds
 Tested Compounds
All(19)
 
 
Active(19)
 
 
 Tested Substances
 Tested Substances
All(19)
 
 
Active(19)
 
 
 Related BioAssays
 Related BioAssays
AID: 725227
Data Source: ChEMBL (937793)
Depositor Category: Literature, Extracted
Deposit Date: 2013-11-07

Data Table ( Complete ):           Active    All
BioActive Compounds: 19
Description:
Title: The extremo-alpha-carbonic anhydrase (CA) from Sulfurihydrogenibium azorense, the fastest CA known, is highly activated by amino acids and amines.

Abstract: The alpha-carbonic anhydrase (CA, EC 4.2.1.1) from the extremophilic bacterium Sulfurihydrogenibium azorense (SazCA) was recently shown to be the fastest CA known. Here we investigated this enzyme for its activation with a series of amino acids and amines. The best SazCA activators were D-Phe, L-DOPA, L- and D-Trp, dopamine and serotonin, which showed activation constants in the range of 3-23 nM. L- and D-His, L-Phe, L-Tyr, 2-pyridyl-methylamine and L-adrenaline were also effective activators (K(A)s in the range of 62-90 nM), whereas D-Dopa, D-Tyr and several heterocyclic amines showed activity in the micromolar range. The good thermal stability, robustness, very high catalytic activity and propensity to be activated by simple amino acids and amines, make SazCA a very interesting candidate for biomimetic CO(2) capture processes.
(PMID: 23294703)
Comment
Compounds with activity <= 50uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Putative Target:

ChEMBL Target ID: 22226
Target Type: UNCHECKED
Pref Name: Unchecked
Confidence: Default value - Target unknown or has yet to be assigned
Relationship Type: Default value - Target has yet to be curated
Categorized Comment
ChEMBL Assay Type: Binding

ChEMBL Assay Data Source: Scientific Literature

Result Definitions
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
1KA activity commentKA activity commentString
2KA standard flagKA standard flagInteger
3KA qualifierKA qualifierString
4KA published valueKA published valueFloatμM
5KA standard valueKA standard valueFloatμM

Data Table (Concise)
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