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BioAssay: AID 720679

qHTS assay to test for compound auto fluorescence at 590 nm (red) in HEK293 cells

Compound auto fluorescence often interferes with screening of the compound library against various biological targets and signaling pathways. Some of these compounds may have fluorophoric properties. Such properties may interfere with the assay outputs resulting in false positives and/or negatives. To decrease such complications and ensure the compound activities are relevant in terms of biological response, the Tox21 10K compound library was screened for auto fluorescence. ..more
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 Tested Compounds
 Tested Compounds
All(8121)
 
 
Active(10)
 
 
Inactive(8105)
 
 
Inconclusive(8)
 
 
 Tested Substances
 Tested Substances
All(10496)
 
 
Active(15)
 
 
Inactive(10473)
 
 
Inconclusive(8)
 
 
 Related BioAssays
 Related BioAssays
AID: 720679
Data Source: Tox21 (SPEC167CR)
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Depositor Category: Other
Deposit Date: 2013-10-24
Modify Date: 2014-08-12

Data Table ( Complete ):           View Active Data    View All Data
BioActive Compounds: 10
Description:
U.S. Tox21 Program

National Center for Advancing Translational Sciences [NCATS]
NIH Chemical Genomics Center [NCGC]
U.S. Environmental Protection Agency [EPA]
National Institutes of Environmental Health Sciences [NIEHS]
National Toxicology Program [NTP]
U.S. Food and Drug Administration [FDA]

Tox21 Assay Overview:

Compound auto fluorescence often interferes with screening of the compound library against various biological targets and signaling pathways. Some of these compounds may have fluorophoric properties. Such properties may interfere with the assay outputs resulting in false positives and/or negatives. To decrease such complications and ensure the compound activities are relevant in terms of biological response, the Tox21 10K compound library was screened for auto fluorescence.

From the fluorophores used as reporter signals in our screening assays, the auto fluorescence equivalencies of compounds against three fluorophores have been evaluated: Triamterene, Fluorescein and Rose Bengal sodium for blue, green and red fluorescence, respectively. The fluorescence intensities were measured using Excitation/Emission wavelengths at 405/460nm, 485/535nm and 540/590nm for blue, green and red labels, respectively. This assay includes the red fluorescence data.
Protocol
Tox21 Assay Protocol Summary:

2,000 HEK293 cells in 5 uL of culture medium containing 10% dialyzed FBS per well were dispensed into black wall/clear bottom 1536-well plates using a Multidrop Combi Dispenser (Thermo Scientific). The assay plates were incubated for 4-5 hr at 37C to allow cell attachment to the well bottom, followed by the transfer of 23 nL compound or DMSO vehicle by a pintool work station (Kalypsys, San Diego, CA). After the assay plates were incubated overnight at 37C and 5% CO2, fluorescence intensities in the assay plates were measured by an Envision (PerkinElmer, Shelton, CT) plate reader using three labels for measuring blue, green and red fluorescence at excitations 460, 535 and 590nm respectively.
Comment
Disclaimer:

Although all reasonable efforts have been made to ensure the accuracy and reliability of the data, caution should be exercised when interpreting the results as artifacts are possible from nonspecific effects such as assay signal interference. The curve fitting and activity calls presented here are based on the NCATS analysis methods. Alternative analysis methods and interpretations of the data are available at EPA (http://actor.epa.gov) and NTP (http://tools.niehs.nih.gov/cebs3/ui/).

Compound Ranking:

1. Compounds are first classified as having full titration curves, partial modulation, partial curve (weaker actives), single point activity (at highest concentration only), or inactive. See data field "Curve Description". For this assay, apparent activators are ranked higher than compounds that showed apparent inhibition.
2. For all inactive compounds, PUBCHEM_ACTIVITY_SCORE is 0. For all active compounds, a score range was given for each curve class type given above. Active compounds have PUBCHEM_ACTIVITY_SCORE between 40 and 100. Inconclusive compounds have PUBCHEM_ACTIVITY_SCORE between 1 and 39. Fit_LogAC50 was used for determining relative score and was scaled to each curve class' score range.
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1Phenotype-Replicate_1Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
2Potency-Replicate_1*Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
3Efficacy-Replicate_1Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
4Analysis Comment-Replicate_1Annotation/notes on a particular compound's data or its analysis.String
5Activity_Score-Replicate_1Activity score.Integer
6Curve_Description-Replicate_1A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
7Fit_LogAC50-Replicate_1The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
8Fit_HillSlope-Replicate_1The Hill slope from a fit of the data to the Hill equation.Float
9Fit_R2-Replicate_1R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
10Fit_InfiniteActivity-Replicate_1The asymptotic efficacy from a fit of the data to the Hill equation.Float%
11Fit_ZeroActivity-Replicate_1Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
12Fit_CurveClass-Replicate_1Numerical encoding of curve description for the fitted Hill equation.Float
13Excluded_Points-Replicate_1Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
14Max_Response-Replicate_1Maximum activity observed for compound (usually at highest concentration tested).Float%
15Activity at 0.0005530000 uM-Replicate_1 (0.000553μM**)% Activity at given concentration.Float%
16Activity at 0.00276 uM-Replicate_1 (0.002765μM**)% Activity at given concentration.Float%
17Activity at 0.00765 uM-Replicate_1 (0.00764923μM**)% Activity at given concentration.Float%
18Activity at 0.012 uM-Replicate_1 (0.0124905μM**)% Activity at given concentration.Float%
19Activity at 0.024 uM-Replicate_1 (0.0241248μM**)% Activity at given concentration.Float%
20Activity at 0.034 uM-Replicate_1 (0.0339475μM**)% Activity at given concentration.Float%
21Activity at 0.074 uM-Replicate_1 (0.0737787μM**)% Activity at given concentration.Float%
22Activity at 0.148 uM-Replicate_1 (0.148068μM**)% Activity at given concentration.Float%
23Activity at 0.199 uM-Replicate_1 (0.199162μM**)% Activity at given concentration.Float%
24Activity at 0.370 uM-Replicate_1 (0.370495μM**)% Activity at given concentration.Float%
25Activity at 0.743 uM-Replicate_1 (0.743135μM**)% Activity at given concentration.Float%
26Activity at 1.207 uM-Replicate_1 (1.20721μM**)% Activity at given concentration.Float%
27Activity at 1.859 uM-Replicate_1 (1.8591μM**)% Activity at given concentration.Float%
28Activity at 3.722 uM-Replicate_1 (3.72239μM**)% Activity at given concentration.Float%
29Activity at 9.170 uM-Replicate_1 (9.16985μM**)% Activity at given concentration.Float%
30Activity at 13.42 uM-Replicate_1 (13.4244μM**)% Activity at given concentration.Float%
31Activity at 18.62 uM-Replicate_1 (18.6188μM**)% Activity at given concentration.Float%
32Activity at 45.97 uM-Replicate_1 (45.9725μM**)% Activity at given concentration.Float%
33Activity at 91.34 uM-Replicate_1 (91.3355μM**)% Activity at given concentration.Float%
34Activity at 95.67 uM-Replicate_1 (95.6717μM**)% Activity at given concentration.Float%
35Activity at 202.9 uM-Replicate_1 (202.913μM**)% Activity at given concentration.Float%
36Activity at 354.0 uM-Replicate_1 (354μM**)% Activity at given concentration.Float%
37Activity at 920.3 uM-Replicate_1 (920.3μM**)% Activity at given concentration.Float%
38Compound QC-Replicate_1NCGC designation for data stage: 'qHTS', 'qHTS Verification', 'Secondary Profiling'String
39Phenotype-Replicate_2Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
40Potency-Replicate_2Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
41Efficacy-Replicate_2Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
42Analysis Comment-Replicate_2Annotation/notes on a particular compound's data or its analysis.String
43Activity_Score-Replicate_2Activity score.Integer
44Curve_Description-Replicate_2A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
45Fit_LogAC50-Replicate_2The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
46Fit_HillSlope-Replicate_2The Hill slope from a fit of the data to the Hill equation.Float
47Fit_R2-Replicate_2R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
48Fit_InfiniteActivity-Replicate_2The asymptotic efficacy from a fit of the data to the Hill equation.Float%
49Fit_ZeroActivity-Replicate_2Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
50Fit_CurveClass-Replicate_2Numerical encoding of curve description for the fitted Hill equation.Float
51Excluded_Points-Replicate_2Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
52Max_Response-Replicate_2Maximum activity observed for compound (usually at highest concentration tested).Float%
53Activity at 0.0005530000 uM-Replicate_2 (0.000553μM**)% Activity at given concentration.Float%
54Activity at 0.00276 uM-Replicate_2 (0.002765μM**)% Activity at given concentration.Float%
55Activity at 0.00765 uM-Replicate_2 (0.00764923μM**)% Activity at given concentration.Float%
56Activity at 0.012 uM-Replicate_2 (0.0124905μM**)% Activity at given concentration.Float%
57Activity at 0.024 uM-Replicate_2 (0.0241248μM**)% Activity at given concentration.Float%
58Activity at 0.034 uM-Replicate_2 (0.0339475μM**)% Activity at given concentration.Float%
59Activity at 0.074 uM-Replicate_2 (0.0737787μM**)% Activity at given concentration.Float%
60Activity at 0.148 uM-Replicate_2 (0.148068μM**)% Activity at given concentration.Float%
61Activity at 0.199 uM-Replicate_2 (0.199162μM**)% Activity at given concentration.Float%
62Activity at 0.370 uM-Replicate_2 (0.370495μM**)% Activity at given concentration.Float%
63Activity at 0.743 uM-Replicate_2 (0.743135μM**)% Activity at given concentration.Float%
64Activity at 1.207 uM-Replicate_2 (1.20721μM**)% Activity at given concentration.Float%
65Activity at 1.859 uM-Replicate_2 (1.8591μM**)% Activity at given concentration.Float%
66Activity at 3.722 uM-Replicate_2 (3.72239μM**)% Activity at given concentration.Float%
67Activity at 9.170 uM-Replicate_2 (9.16985μM**)% Activity at given concentration.Float%
68Activity at 13.42 uM-Replicate_2 (13.4244μM**)% Activity at given concentration.Float%
69Activity at 18.62 uM-Replicate_2 (18.6188μM**)% Activity at given concentration.Float%
70Activity at 45.97 uM-Replicate_2 (45.9725μM**)% Activity at given concentration.Float%
71Activity at 91.34 uM-Replicate_2 (91.3355μM**)% Activity at given concentration.Float%
72Activity at 95.67 uM-Replicate_2 (95.6717μM**)% Activity at given concentration.Float%
73Activity at 202.9 uM-Replicate_2 (202.913μM**)% Activity at given concentration.Float%
74Activity at 354.0 uM-Replicate_2 (354μM**)% Activity at given concentration.Float%
75Activity at 920.3 uM-Replicate_2 (920.3μM**)% Activity at given concentration.Float%
76Phenotype-Replicate_3Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
77Potency-Replicate_3Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
78Efficacy-Replicate_3Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
79Analysis Comment-Replicate_3Annotation/notes on a particular compound's data or its analysis.String
80Activity_Score-Replicate_3Activity score.Integer
81Curve_Description-Replicate_3A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
82Fit_LogAC50-Replicate_3The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
83Fit_HillSlope-Replicate_3The Hill slope from a fit of the data to the Hill equation.Float
84Fit_R2-Replicate_3R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
85Fit_InfiniteActivity-Replicate_3The asymptotic efficacy from a fit of the data to the Hill equation.Float%
86Fit_ZeroActivity-Replicate_3Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
87Fit_CurveClass-Replicate_3Numerical encoding of curve description for the fitted Hill equation.Float
88Excluded_Points-Replicate_3Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
89Max_Response-Replicate_3Maximum activity observed for compound (usually at highest concentration tested).Float%
90Activity at 0.0005530000 uM-Replicate_3 (0.000553μM**)% Activity at given concentration.Float%
91Activity at 0.00276 uM-Replicate_3 (0.002765μM**)% Activity at given concentration.Float%
92Activity at 0.00765 uM-Replicate_3 (0.00764923μM**)% Activity at given concentration.Float%
93Activity at 0.012 uM-Replicate_3 (0.0124905μM**)% Activity at given concentration.Float%
94Activity at 0.024 uM-Replicate_3 (0.0241248μM**)% Activity at given concentration.Float%
95Activity at 0.034 uM-Replicate_3 (0.0339475μM**)% Activity at given concentration.Float%
96Activity at 0.074 uM-Replicate_3 (0.0737787μM**)% Activity at given concentration.Float%
97Activity at 0.148 uM-Replicate_3 (0.148068μM**)% Activity at given concentration.Float%
98Activity at 0.199 uM-Replicate_3 (0.199162μM**)% Activity at given concentration.Float%
99Activity at 0.370 uM-Replicate_3 (0.370495μM**)% Activity at given concentration.Float%
100Activity at 0.743 uM-Replicate_3 (0.743135μM**)% Activity at given concentration.Float%
101Activity at 1.207 uM-Replicate_3 (1.20721μM**)% Activity at given concentration.Float%
102Activity at 1.859 uM-Replicate_3 (1.8591μM**)% Activity at given concentration.Float%
103Activity at 3.722 uM-Replicate_3 (3.72239μM**)% Activity at given concentration.Float%
104Activity at 9.170 uM-Replicate_3 (9.16985μM**)% Activity at given concentration.Float%
105Activity at 13.42 uM-Replicate_3 (13.4244μM**)% Activity at given concentration.Float%
106Activity at 18.62 uM-Replicate_3 (18.6188μM**)% Activity at given concentration.Float%
107Activity at 45.97 uM-Replicate_3 (45.9725μM**)% Activity at given concentration.Float%
108Activity at 91.34 uM-Replicate_3 (91.3355μM**)% Activity at given concentration.Float%
109Activity at 95.67 uM-Replicate_3 (95.6717μM**)% Activity at given concentration.Float%
110Activity at 202.9 uM-Replicate_3 (202.913μM**)% Activity at given concentration.Float%
111Activity at 354.0 uM-Replicate_3 (354μM**)% Activity at given concentration.Float%
112Activity at 920.3 uM-Replicate_3 (920.3μM**)% Activity at given concentration.Float%
113Phenotype-Replicate_4Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
114Potency-Replicate_4Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
115Efficacy-Replicate_4Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
116Analysis Comment-Replicate_4Annotation/notes on a particular compound's data or its analysis.String
117Activity_Score-Replicate_4Activity score.Integer
118Curve_Description-Replicate_4A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
119Fit_LogAC50-Replicate_4The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
120Fit_HillSlope-Replicate_4The Hill slope from a fit of the data to the Hill equation.Float
121Fit_R2-Replicate_4R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
122Fit_InfiniteActivity-Replicate_4The asymptotic efficacy from a fit of the data to the Hill equation.Float%
123Fit_ZeroActivity-Replicate_4Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
124Fit_CurveClass-Replicate_4Numerical encoding of curve description for the fitted Hill equation.Float
125Excluded_Points-Replicate_4Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
126Max_Response-Replicate_4Maximum activity observed for compound (usually at highest concentration tested).Float%
127Activity at 0.0005530000 uM-Replicate_4 (0.000553μM**)% Activity at given concentration.Float%
128Activity at 0.00276 uM-Replicate_4 (0.002765μM**)% Activity at given concentration.Float%
129Activity at 0.00765 uM-Replicate_4 (0.00764923μM**)% Activity at given concentration.Float%
130Activity at 0.012 uM-Replicate_4 (0.0124905μM**)% Activity at given concentration.Float%
131Activity at 0.024 uM-Replicate_4 (0.0241248μM**)% Activity at given concentration.Float%
132Activity at 0.034 uM-Replicate_4 (0.0339475μM**)% Activity at given concentration.Float%
133Activity at 0.074 uM-Replicate_4 (0.0737787μM**)% Activity at given concentration.Float%
134Activity at 0.148 uM-Replicate_4 (0.148068μM**)% Activity at given concentration.Float%
135Activity at 0.199 uM-Replicate_4 (0.199162μM**)% Activity at given concentration.Float%
136Activity at 0.370 uM-Replicate_4 (0.370495μM**)% Activity at given concentration.Float%
137Activity at 0.743 uM-Replicate_4 (0.743135μM**)% Activity at given concentration.Float%
138Activity at 1.207 uM-Replicate_4 (1.20721μM**)% Activity at given concentration.Float%
139Activity at 1.859 uM-Replicate_4 (1.8591μM**)% Activity at given concentration.Float%
140Activity at 3.722 uM-Replicate_4 (3.72239μM**)% Activity at given concentration.Float%
141Activity at 9.170 uM-Replicate_4 (9.16985μM**)% Activity at given concentration.Float%
142Activity at 13.42 uM-Replicate_4 (13.4244μM**)% Activity at given concentration.Float%
143Activity at 18.62 uM-Replicate_4 (18.6188μM**)% Activity at given concentration.Float%
144Activity at 45.97 uM-Replicate_4 (45.9725μM**)% Activity at given concentration.Float%
145Activity at 91.34 uM-Replicate_4 (91.3355μM**)% Activity at given concentration.Float%
146Activity at 95.67 uM-Replicate_4 (95.6717μM**)% Activity at given concentration.Float%
147Activity at 202.9 uM-Replicate_4 (202.913μM**)% Activity at given concentration.Float%
148Activity at 354.0 uM-Replicate_4 (354μM**)% Activity at given concentration.Float%
149Activity at 920.3 uM-Replicate_4 (920.3μM**)% Activity at given concentration.Float%
150Phenotype-Replicate_5Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
151Potency-Replicate_5Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
152Efficacy-Replicate_5Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
153Analysis Comment-Replicate_5Annotation/notes on a particular compound's data or its analysis.String
154Activity_Score-Replicate_5Activity score.Integer
155Curve_Description-Replicate_5A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
156Fit_LogAC50-Replicate_5The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
157Fit_HillSlope-Replicate_5The Hill slope from a fit of the data to the Hill equation.Float
158Fit_R2-Replicate_5R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
159Fit_InfiniteActivity-Replicate_5The asymptotic efficacy from a fit of the data to the Hill equation.Float%
160Fit_ZeroActivity-Replicate_5Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
161Fit_CurveClass-Replicate_5Numerical encoding of curve description for the fitted Hill equation.Float
162Excluded_Points-Replicate_5Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
163Max_Response-Replicate_5Maximum activity observed for compound (usually at highest concentration tested).Float%
164Activity at 0.0005530000 uM-Replicate_5 (0.000553μM**)% Activity at given concentration.Float%
165Activity at 0.00276 uM-Replicate_5 (0.002765μM**)% Activity at given concentration.Float%
166Activity at 0.00765 uM-Replicate_5 (0.00764923μM**)% Activity at given concentration.Float%
167Activity at 0.012 uM-Replicate_5 (0.0124905μM**)% Activity at given concentration.Float%
168Activity at 0.024 uM-Replicate_5 (0.0241248μM**)% Activity at given concentration.Float%
169Activity at 0.034 uM-Replicate_5 (0.0339475μM**)% Activity at given concentration.Float%
170Activity at 0.074 uM-Replicate_5 (0.0737787μM**)% Activity at given concentration.Float%
171Activity at 0.148 uM-Replicate_5 (0.148068μM**)% Activity at given concentration.Float%
172Activity at 0.199 uM-Replicate_5 (0.199162μM**)% Activity at given concentration.Float%
173Activity at 0.370 uM-Replicate_5 (0.370495μM**)% Activity at given concentration.Float%
174Activity at 0.743 uM-Replicate_5 (0.743135μM**)% Activity at given concentration.Float%
175Activity at 1.207 uM-Replicate_5 (1.20721μM**)% Activity at given concentration.Float%
176Activity at 1.859 uM-Replicate_5 (1.8591μM**)% Activity at given concentration.Float%
177Activity at 3.722 uM-Replicate_5 (3.72239μM**)% Activity at given concentration.Float%
178Activity at 9.170 uM-Replicate_5 (9.16985μM**)% Activity at given concentration.Float%
179Activity at 13.42 uM-Replicate_5 (13.4244μM**)% Activity at given concentration.Float%
180Activity at 18.62 uM-Replicate_5 (18.6188μM**)% Activity at given concentration.Float%
181Activity at 45.97 uM-Replicate_5 (45.9725μM**)% Activity at given concentration.Float%
182Activity at 91.34 uM-Replicate_5 (91.3355μM**)% Activity at given concentration.Float%
183Activity at 95.67 uM-Replicate_5 (95.6717μM**)% Activity at given concentration.Float%
184Activity at 202.9 uM-Replicate_5 (202.913μM**)% Activity at given concentration.Float%
185Activity at 354.0 uM-Replicate_5 (354μM**)% Activity at given concentration.Float%
186Activity at 920.3 uM-Replicate_5 (920.3μM**)% Activity at given concentration.Float%
187Phenotype-Replicate_6Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
188Potency-Replicate_6Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
189Efficacy-Replicate_6Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
190Analysis Comment-Replicate_6Annotation/notes on a particular compound's data or its analysis.String
191Activity_Score-Replicate_6Activity score.Integer
192Curve_Description-Replicate_6A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
193Fit_LogAC50-Replicate_6The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
194Fit_HillSlope-Replicate_6The Hill slope from a fit of the data to the Hill equation.Float
195Fit_R2-Replicate_6R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
196Fit_InfiniteActivity-Replicate_6The asymptotic efficacy from a fit of the data to the Hill equation.Float%
197Fit_ZeroActivity-Replicate_6Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
198Fit_CurveClass-Replicate_6Numerical encoding of curve description for the fitted Hill equation.Float
199Excluded_Points-Replicate_6Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
200Max_Response-Replicate_6Maximum activity observed for compound (usually at highest concentration tested).Float%
201Activity at 0.0005530000 uM-Replicate_6 (0.000553μM**)% Activity at given concentration.Float%
202Activity at 0.00276 uM-Replicate_6 (0.002765μM**)% Activity at given concentration.Float%
203Activity at 0.00765 uM-Replicate_6 (0.00764923μM**)% Activity at given concentration.Float%
204Activity at 0.012 uM-Replicate_6 (0.0124905μM**)% Activity at given concentration.Float%
205Activity at 0.024 uM-Replicate_6 (0.0241248μM**)% Activity at given concentration.Float%
206Activity at 0.034 uM-Replicate_6 (0.0339475μM**)% Activity at given concentration.Float%
207Activity at 0.074 uM-Replicate_6 (0.0737787μM**)% Activity at given concentration.Float%
208Activity at 0.148 uM-Replicate_6 (0.148068μM**)% Activity at given concentration.Float%
209Activity at 0.199 uM-Replicate_6 (0.199162μM**)% Activity at given concentration.Float%
210Activity at 0.370 uM-Replicate_6 (0.370495μM**)% Activity at given concentration.Float%
211Activity at 0.743 uM-Replicate_6 (0.743135μM**)% Activity at given concentration.Float%
212Activity at 1.207 uM-Replicate_6 (1.20721μM**)% Activity at given concentration.Float%
213Activity at 1.859 uM-Replicate_6 (1.8591μM**)% Activity at given concentration.Float%
214Activity at 3.722 uM-Replicate_6 (3.72239μM**)% Activity at given concentration.Float%
215Activity at 9.170 uM-Replicate_6 (9.16985μM**)% Activity at given concentration.Float%
216Activity at 13.42 uM-Replicate_6 (13.4244μM**)% Activity at given concentration.Float%
217Activity at 18.62 uM-Replicate_6 (18.6188μM**)% Activity at given concentration.Float%
218Activity at 45.97 uM-Replicate_6 (45.9725μM**)% Activity at given concentration.Float%
219Activity at 91.34 uM-Replicate_6 (91.3355μM**)% Activity at given concentration.Float%
220Activity at 95.67 uM-Replicate_6 (95.6717μM**)% Activity at given concentration.Float%
221Activity at 202.9 uM-Replicate_6 (202.913μM**)% Activity at given concentration.Float%
222Activity at 354.0 uM-Replicate_6 (354μM**)% Activity at given concentration.Float%
223Activity at 920.3 uM-Replicate_6 (920.3μM**)% Activity at given concentration.Float%
224Phenotype-Replicate_7Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
225Potency-Replicate_7Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
226Efficacy-Replicate_7Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
227Analysis Comment-Replicate_7Annotation/notes on a particular compound's data or its analysis.String
228Activity_Score-Replicate_7Activity score.Integer
229Curve_Description-Replicate_7A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
230Fit_LogAC50-Replicate_7The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
231Fit_HillSlope-Replicate_7The Hill slope from a fit of the data to the Hill equation.Float
232Fit_R2-Replicate_7R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
233Fit_InfiniteActivity-Replicate_7The asymptotic efficacy from a fit of the data to the Hill equation.Float%
234Fit_ZeroActivity-Replicate_7Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
235Fit_CurveClass-Replicate_7Numerical encoding of curve description for the fitted Hill equation.Float
236Excluded_Points-Replicate_7Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
237Max_Response-Replicate_7Maximum activity observed for compound (usually at highest concentration tested).Float%
238Activity at 0.0005530000 uM-Replicate_7 (0.000553μM**)% Activity at given concentration.Float%
239Activity at 0.00276 uM-Replicate_7 (0.002765μM**)% Activity at given concentration.Float%
240Activity at 0.00765 uM-Replicate_7 (0.00764923μM**)% Activity at given concentration.Float%
241Activity at 0.012 uM-Replicate_7 (0.0124905μM**)% Activity at given concentration.Float%
242Activity at 0.024 uM-Replicate_7 (0.0241248μM**)% Activity at given concentration.Float%
243Activity at 0.034 uM-Replicate_7 (0.0339475μM**)% Activity at given concentration.Float%
244Activity at 0.074 uM-Replicate_7 (0.0737787μM**)% Activity at given concentration.Float%
245Activity at 0.148 uM-Replicate_7 (0.148068μM**)% Activity at given concentration.Float%
246Activity at 0.199 uM-Replicate_7 (0.199162μM**)% Activity at given concentration.Float%
247Activity at 0.370 uM-Replicate_7 (0.370495μM**)% Activity at given concentration.Float%
248Activity at 0.743 uM-Replicate_7 (0.743135μM**)% Activity at given concentration.Float%
249Activity at 1.207 uM-Replicate_7 (1.20721μM**)% Activity at given concentration.Float%
250Activity at 1.859 uM-Replicate_7 (1.8591μM**)% Activity at given concentration.Float%
251Activity at 3.722 uM-Replicate_7 (3.72239μM**)% Activity at given concentration.Float%
252Activity at 9.170 uM-Replicate_7 (9.16985μM**)% Activity at given concentration.Float%
253Activity at 13.42 uM-Replicate_7 (13.4244μM**)% Activity at given concentration.Float%
254Activity at 18.62 uM-Replicate_7 (18.6188μM**)% Activity at given concentration.Float%
255Activity at 45.97 uM-Replicate_7 (45.9725μM**)% Activity at given concentration.Float%
256Activity at 91.34 uM-Replicate_7 (91.3355μM**)% Activity at given concentration.Float%
257Activity at 95.67 uM-Replicate_7 (95.6717μM**)% Activity at given concentration.Float%
258Activity at 202.9 uM-Replicate_7 (202.913μM**)% Activity at given concentration.Float%
259Activity at 354.0 uM-Replicate_7 (354μM**)% Activity at given concentration.Float%
260Activity at 920.3 uM-Replicate_7 (920.3μM**)% Activity at given concentration.Float%
261Phenotype-Replicate_8Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
262Potency-Replicate_8Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
263Efficacy-Replicate_8Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
264Analysis Comment-Replicate_8Annotation/notes on a particular compound's data or its analysis.String
265Activity_Score-Replicate_8Activity score.Integer
266Curve_Description-Replicate_8A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
267Fit_LogAC50-Replicate_8The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
268Fit_HillSlope-Replicate_8The Hill slope from a fit of the data to the Hill equation.Float
269Fit_R2-Replicate_8R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
270Fit_InfiniteActivity-Replicate_8The asymptotic efficacy from a fit of the data to the Hill equation.Float%
271Fit_ZeroActivity-Replicate_8Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
272Fit_CurveClass-Replicate_8Numerical encoding of curve description for the fitted Hill equation.Float
273Excluded_Points-Replicate_8Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
274Max_Response-Replicate_8Maximum activity observed for compound (usually at highest concentration tested).Float%
275Activity at 0.0005530000 uM-Replicate_8 (0.000553μM**)% Activity at given concentration.Float%
276Activity at 0.00276 uM-Replicate_8 (0.002765μM**)% Activity at given concentration.Float%
277Activity at 0.00765 uM-Replicate_8 (0.00764923μM**)% Activity at given concentration.Float%
278Activity at 0.012 uM-Replicate_8 (0.0124905μM**)% Activity at given concentration.Float%
279Activity at 0.024 uM-Replicate_8 (0.0241248μM**)% Activity at given concentration.Float%
280Activity at 0.034 uM-Replicate_8 (0.0339475μM**)% Activity at given concentration.Float%
281Activity at 0.074 uM-Replicate_8 (0.0737787μM**)% Activity at given concentration.Float%
282Activity at 0.148 uM-Replicate_8 (0.148068μM**)% Activity at given concentration.Float%
283Activity at 0.199 uM-Replicate_8 (0.199162μM**)% Activity at given concentration.Float%
284Activity at 0.370 uM-Replicate_8 (0.370495μM**)% Activity at given concentration.Float%
285Activity at 0.743 uM-Replicate_8 (0.743135μM**)% Activity at given concentration.Float%
286Activity at 1.207 uM-Replicate_8 (1.20721μM**)% Activity at given concentration.Float%
287Activity at 1.859 uM-Replicate_8 (1.8591μM**)% Activity at given concentration.Float%
288Activity at 3.722 uM-Replicate_8 (3.72239μM**)% Activity at given concentration.Float%
289Activity at 9.170 uM-Replicate_8 (9.16985μM**)% Activity at given concentration.Float%
290Activity at 13.42 uM-Replicate_8 (13.4244μM**)% Activity at given concentration.Float%
291Activity at 18.62 uM-Replicate_8 (18.6188μM**)% Activity at given concentration.Float%
292Activity at 45.97 uM-Replicate_8 (45.9725μM**)% Activity at given concentration.Float%
293Activity at 91.34 uM-Replicate_8 (91.3355μM**)% Activity at given concentration.Float%
294Activity at 95.67 uM-Replicate_8 (95.6717μM**)% Activity at given concentration.Float%
295Activity at 202.9 uM-Replicate_8 (202.913μM**)% Activity at given concentration.Float%
296Activity at 354.0 uM-Replicate_8 (354μM**)% Activity at given concentration.Float%
297Activity at 920.3 uM-Replicate_8 (920.3μM**)% Activity at given concentration.Float%
298Phenotype-Replicate_9Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
299Potency-Replicate_9Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
300Efficacy-Replicate_9Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
301Analysis Comment-Replicate_9Annotation/notes on a particular compound's data or its analysis.String
302Activity_Score-Replicate_9Activity score.Integer
303Curve_Description-Replicate_9A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
304Fit_LogAC50-Replicate_9The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
305Fit_HillSlope-Replicate_9The Hill slope from a fit of the data to the Hill equation.Float
306Fit_R2-Replicate_9R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
307Fit_InfiniteActivity-Replicate_9The asymptotic efficacy from a fit of the data to the Hill equation.Float%
308Fit_ZeroActivity-Replicate_9Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
309Fit_CurveClass-Replicate_9Numerical encoding of curve description for the fitted Hill equation.Float
310Excluded_Points-Replicate_9Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
311Max_Response-Replicate_9Maximum activity observed for compound (usually at highest concentration tested).Float%
312Activity at 0.0005530000 uM-Replicate_9 (0.000553μM**)% Activity at given concentration.Float%
313Activity at 0.00276 uM-Replicate_9 (0.002765μM**)% Activity at given concentration.Float%
314Activity at 0.00765 uM-Replicate_9 (0.00764923μM**)% Activity at given concentration.Float%
315Activity at 0.012 uM-Replicate_9 (0.0124905μM**)% Activity at given concentration.Float%
316Activity at 0.024 uM-Replicate_9 (0.0241248μM**)% Activity at given concentration.Float%
317Activity at 0.034 uM-Replicate_9 (0.0339475μM**)% Activity at given concentration.Float%
318Activity at 0.074 uM-Replicate_9 (0.0737787μM**)% Activity at given concentration.Float%
319Activity at 0.148 uM-Replicate_9 (0.148068μM**)% Activity at given concentration.Float%
320Activity at 0.199 uM-Replicate_9 (0.199162μM**)% Activity at given concentration.Float%
321Activity at 0.370 uM-Replicate_9 (0.370495μM**)% Activity at given concentration.Float%
322Activity at 0.743 uM-Replicate_9 (0.743135μM**)% Activity at given concentration.Float%
323Activity at 1.207 uM-Replicate_9 (1.20721μM**)% Activity at given concentration.Float%
324Activity at 1.859 uM-Replicate_9 (1.8591μM**)% Activity at given concentration.Float%
325Activity at 3.722 uM-Replicate_9 (3.72239μM**)% Activity at given concentration.Float%
326Activity at 9.170 uM-Replicate_9 (9.16985μM**)% Activity at given concentration.Float%
327Activity at 13.42 uM-Replicate_9 (13.4244μM**)% Activity at given concentration.Float%
328Activity at 18.62 uM-Replicate_9 (18.6188μM**)% Activity at given concentration.Float%
329Activity at 45.97 uM-Replicate_9 (45.9725μM**)% Activity at given concentration.Float%
330Activity at 91.34 uM-Replicate_9 (91.3355μM**)% Activity at given concentration.Float%
331Activity at 95.67 uM-Replicate_9 (95.6717μM**)% Activity at given concentration.Float%
332Activity at 202.9 uM-Replicate_9 (202.913μM**)% Activity at given concentration.Float%
333Activity at 354.0 uM-Replicate_9 (354μM**)% Activity at given concentration.Float%
334Activity at 920.3 uM-Replicate_9 (920.3μM**)% Activity at given concentration.Float%
335Phenotype-Replicate_10Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
336Potency-Replicate_10Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
337Efficacy-Replicate_10Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
338Analysis Comment-Replicate_10Annotation/notes on a particular compound's data or its analysis.String
339Activity_Score-Replicate_10Activity score.Integer
340Curve_Description-Replicate_10A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
341Fit_LogAC50-Replicate_10The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
342Fit_HillSlope-Replicate_10The Hill slope from a fit of the data to the Hill equation.Float
343Fit_R2-Replicate_10R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
344Fit_InfiniteActivity-Replicate_10The asymptotic efficacy from a fit of the data to the Hill equation.Float%
345Fit_ZeroActivity-Replicate_10Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
346Fit_CurveClass-Replicate_10Numerical encoding of curve description for the fitted Hill equation.Float
347Excluded_Points-Replicate_10Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
348Max_Response-Replicate_10Maximum activity observed for compound (usually at highest concentration tested).Float%
349Activity at 0.0005530000 uM-Replicate_10 (0.000553μM**)% Activity at given concentration.Float%
350Activity at 0.00276 uM-Replicate_10 (0.002765μM**)% Activity at given concentration.Float%
351Activity at 0.00765 uM-Replicate_10 (0.00764923μM**)% Activity at given concentration.Float%
352Activity at 0.012 uM-Replicate_10 (0.0124905μM**)% Activity at given concentration.Float%
353Activity at 0.024 uM-Replicate_10 (0.0241248μM**)% Activity at given concentration.Float%
354Activity at 0.034 uM-Replicate_10 (0.0339475μM**)% Activity at given concentration.Float%
355Activity at 0.074 uM-Replicate_10 (0.0737787μM**)% Activity at given concentration.Float%
356Activity at 0.148 uM-Replicate_10 (0.148068μM**)% Activity at given concentration.Float%
357Activity at 0.199 uM-Replicate_10 (0.199162μM**)% Activity at given concentration.Float%
358Activity at 0.370 uM-Replicate_10 (0.370495μM**)% Activity at given concentration.Float%
359Activity at 0.743 uM-Replicate_10 (0.743135μM**)% Activity at given concentration.Float%
360Activity at 1.207 uM-Replicate_10 (1.20721μM**)% Activity at given concentration.Float%
361Activity at 1.859 uM-Replicate_10 (1.8591μM**)% Activity at given concentration.Float%
362Activity at 3.722 uM-Replicate_10 (3.72239μM**)% Activity at given concentration.Float%
363Activity at 9.170 uM-Replicate_10 (9.16985μM**)% Activity at given concentration.Float%
364Activity at 13.42 uM-Replicate_10 (13.4244μM**)% Activity at given concentration.Float%
365Activity at 18.62 uM-Replicate_10 (18.6188μM**)% Activity at given concentration.Float%
366Activity at 45.97 uM-Replicate_10 (45.9725μM**)% Activity at given concentration.Float%
367Activity at 91.34 uM-Replicate_10 (91.3355μM**)% Activity at given concentration.Float%
368Activity at 95.67 uM-Replicate_10 (95.6717μM**)% Activity at given concentration.Float%
369Activity at 202.9 uM-Replicate_10 (202.913μM**)% Activity at given concentration.Float%
370Activity at 354.0 uM-Replicate_10 (354μM**)% Activity at given concentration.Float%
371Activity at 920.3 uM-Replicate_10 (920.3μM**)% Activity at given concentration.Float%
372Phenotype-Replicate_11Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
373Potency-Replicate_11Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
374Efficacy-Replicate_11Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
375Analysis Comment-Replicate_11Annotation/notes on a particular compound's data or its analysis.String
376Activity_Score-Replicate_11Activity score.Integer
377Curve_Description-Replicate_11A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
378Fit_LogAC50-Replicate_11The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
379Fit_HillSlope-Replicate_11The Hill slope from a fit of the data to the Hill equation.Float
380Fit_R2-Replicate_11R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
381Fit_InfiniteActivity-Replicate_11The asymptotic efficacy from a fit of the data to the Hill equation.Float%
382Fit_ZeroActivity-Replicate_11Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
383Fit_CurveClass-Replicate_11Numerical encoding of curve description for the fitted Hill equation.Float
384Excluded_Points-Replicate_11Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
385Max_Response-Replicate_11Maximum activity observed for compound (usually at highest concentration tested).Float%
386Activity at 0.0005530000 uM-Replicate_11 (0.000553μM**)% Activity at given concentration.Float%
387Activity at 0.00276 uM-Replicate_11 (0.002765μM**)% Activity at given concentration.Float%
388Activity at 0.00765 uM-Replicate_11 (0.00764923μM**)% Activity at given concentration.Float%
389Activity at 0.012 uM-Replicate_11 (0.0124905μM**)% Activity at given concentration.Float%
390Activity at 0.024 uM-Replicate_11 (0.0241248μM**)% Activity at given concentration.Float%
391Activity at 0.034 uM-Replicate_11 (0.0339475μM**)% Activity at given concentration.Float%
392Activity at 0.074 uM-Replicate_11 (0.0737787μM**)% Activity at given concentration.Float%
393Activity at 0.148 uM-Replicate_11 (0.148068μM**)% Activity at given concentration.Float%
394Activity at 0.199 uM-Replicate_11 (0.199162μM**)% Activity at given concentration.Float%
395Activity at 0.370 uM-Replicate_11 (0.370495μM**)% Activity at given concentration.Float%
396Activity at 0.743 uM-Replicate_11 (0.743135μM**)% Activity at given concentration.Float%
397Activity at 1.207 uM-Replicate_11 (1.20721μM**)% Activity at given concentration.Float%
398Activity at 1.859 uM-Replicate_11 (1.8591μM**)% Activity at given concentration.Float%
399Activity at 3.722 uM-Replicate_11 (3.72239μM**)% Activity at given concentration.Float%
400Activity at 9.170 uM-Replicate_11 (9.16985μM**)% Activity at given concentration.Float%
401Activity at 13.42 uM-Replicate_11 (13.4244μM**)% Activity at given concentration.Float%
402Activity at 18.62 uM-Replicate_11 (18.6188μM**)% Activity at given concentration.Float%
403Activity at 45.97 uM-Replicate_11 (45.9725μM**)% Activity at given concentration.Float%
404Activity at 91.34 uM-Replicate_11 (91.3355μM**)% Activity at given concentration.Float%
405Activity at 95.67 uM-Replicate_11 (95.6717μM**)% Activity at given concentration.Float%
406Activity at 202.9 uM-Replicate_11 (202.913μM**)% Activity at given concentration.Float%
407Activity at 354.0 uM-Replicate_11 (354μM**)% Activity at given concentration.Float%
408Activity at 920.3 uM-Replicate_11 (920.3μM**)% Activity at given concentration.Float%
409Phenotype-Replicate_12Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
410Potency-Replicate_12Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
411Efficacy-Replicate_12Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
412Analysis Comment-Replicate_12Annotation/notes on a particular compound's data or its analysis.String
413Activity_Score-Replicate_12Activity score.Integer
414Curve_Description-Replicate_12A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
415Fit_LogAC50-Replicate_12The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
416Fit_HillSlope-Replicate_12The Hill slope from a fit of the data to the Hill equation.Float
417Fit_R2-Replicate_12R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
418Fit_InfiniteActivity-Replicate_12The asymptotic efficacy from a fit of the data to the Hill equation.Float%
419Fit_ZeroActivity-Replicate_12Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
420Fit_CurveClass-Replicate_12Numerical encoding of curve description for the fitted Hill equation.Float
421Excluded_Points-Replicate_12Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
422Max_Response-Replicate_12Maximum activity observed for compound (usually at highest concentration tested).Float%
423Activity at 0.0005530000 uM-Replicate_12 (0.000553μM**)% Activity at given concentration.Float%
424Activity at 0.00276 uM-Replicate_12 (0.002765μM**)% Activity at given concentration.Float%
425Activity at 0.00765 uM-Replicate_12 (0.00764923μM**)% Activity at given concentration.Float%
426Activity at 0.012 uM-Replicate_12 (0.0124905μM**)% Activity at given concentration.Float%
427Activity at 0.024 uM-Replicate_12 (0.0241248μM**)% Activity at given concentration.Float%
428Activity at 0.034 uM-Replicate_12 (0.0339475μM**)% Activity at given concentration.Float%
429Activity at 0.074 uM-Replicate_12 (0.0737787μM**)% Activity at given concentration.Float%
430Activity at 0.148 uM-Replicate_12 (0.148068μM**)% Activity at given concentration.Float%
431Activity at 0.199 uM-Replicate_12 (0.199162μM**)% Activity at given concentration.Float%
432Activity at 0.370 uM-Replicate_12 (0.370495μM**)% Activity at given concentration.Float%
433Activity at 0.743 uM-Replicate_12 (0.743135μM**)% Activity at given concentration.Float%
434Activity at 1.207 uM-Replicate_12 (1.20721μM**)% Activity at given concentration.Float%
435Activity at 1.859 uM-Replicate_12 (1.8591μM**)% Activity at given concentration.Float%
436Activity at 3.722 uM-Replicate_12 (3.72239μM**)% Activity at given concentration.Float%
437Activity at 9.170 uM-Replicate_12 (9.16985μM**)% Activity at given concentration.Float%
438Activity at 13.42 uM-Replicate_12 (13.4244μM**)% Activity at given concentration.Float%
439Activity at 18.62 uM-Replicate_12 (18.6188μM**)% Activity at given concentration.Float%
440Activity at 45.97 uM-Replicate_12 (45.9725μM**)% Activity at given concentration.Float%
441Activity at 91.34 uM-Replicate_12 (91.3355μM**)% Activity at given concentration.Float%
442Activity at 95.67 uM-Replicate_12 (95.6717μM**)% Activity at given concentration.Float%
443Activity at 202.9 uM-Replicate_12 (202.913μM**)% Activity at given concentration.Float%
444Activity at 354.0 uM-Replicate_12 (354μM**)% Activity at given concentration.Float%
445Activity at 920.3 uM-Replicate_12 (920.3μM**)% Activity at given concentration.Float%
446Phenotype-Replicate_13Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
447Potency-Replicate_13Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
448Efficacy-Replicate_13Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
449Analysis Comment-Replicate_13Annotation/notes on a particular compound's data or its analysis.String
450Activity_Score-Replicate_13Activity score.Integer
451Curve_Description-Replicate_13A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
452Fit_LogAC50-Replicate_13The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
453Fit_HillSlope-Replicate_13The Hill slope from a fit of the data to the Hill equation.Float
454Fit_R2-Replicate_13R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
455Fit_InfiniteActivity-Replicate_13The asymptotic efficacy from a fit of the data to the Hill equation.Float%
456Fit_ZeroActivity-Replicate_13Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
457Fit_CurveClass-Replicate_13Numerical encoding of curve description for the fitted Hill equation.Float
458Excluded_Points-Replicate_13Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
459Max_Response-Replicate_13Maximum activity observed for compound (usually at highest concentration tested).Float%
460Activity at 0.0005530000 uM-Replicate_13 (0.000553μM**)% Activity at given concentration.Float%
461Activity at 0.00276 uM-Replicate_13 (0.002765μM**)% Activity at given concentration.Float%
462Activity at 0.00765 uM-Replicate_13 (0.00764923μM**)% Activity at given concentration.Float%
463Activity at 0.012 uM-Replicate_13 (0.0124905μM**)% Activity at given concentration.Float%
464Activity at 0.024 uM-Replicate_13 (0.0241248μM**)% Activity at given concentration.Float%
465Activity at 0.034 uM-Replicate_13 (0.0339475μM**)% Activity at given concentration.Float%
466Activity at 0.074 uM-Replicate_13 (0.0737787μM**)% Activity at given concentration.Float%
467Activity at 0.148 uM-Replicate_13 (0.148068μM**)% Activity at given concentration.Float%
468Activity at 0.199 uM-Replicate_13 (0.199162μM**)% Activity at given concentration.Float%
469Activity at 0.370 uM-Replicate_13 (0.370495μM**)% Activity at given concentration.Float%
470Activity at 0.743 uM-Replicate_13 (0.743135μM**)% Activity at given concentration.Float%
471Activity at 1.207 uM-Replicate_13 (1.20721μM**)% Activity at given concentration.Float%
472Activity at 1.859 uM-Replicate_13 (1.8591μM**)% Activity at given concentration.Float%
473Activity at 3.722 uM-Replicate_13 (3.72239μM**)% Activity at given concentration.Float%
474Activity at 9.170 uM-Replicate_13 (9.16985μM**)% Activity at given concentration.Float%
475Activity at 13.42 uM-Replicate_13 (13.4244μM**)% Activity at given concentration.Float%
476Activity at 18.62 uM-Replicate_13 (18.6188μM**)% Activity at given concentration.Float%
477Activity at 45.97 uM-Replicate_13 (45.9725μM**)% Activity at given concentration.Float%
478Activity at 91.34 uM-Replicate_13 (91.3355μM**)% Activity at given concentration.Float%
479Activity at 95.67 uM-Replicate_13 (95.6717μM**)% Activity at given concentration.Float%
480Activity at 202.9 uM-Replicate_13 (202.913μM**)% Activity at given concentration.Float%
481Activity at 354.0 uM-Replicate_13 (354μM**)% Activity at given concentration.Float%
482Activity at 920.3 uM-Replicate_13 (920.3μM**)% Activity at given concentration.Float%
483Phenotype-Replicate_14Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
484Potency-Replicate_14Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
485Efficacy-Replicate_14Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
486Analysis Comment-Replicate_14Annotation/notes on a particular compound's data or its analysis.String
487Activity_Score-Replicate_14Activity score.Integer
488Curve_Description-Replicate_14A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
489Fit_LogAC50-Replicate_14The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
490Fit_HillSlope-Replicate_14The Hill slope from a fit of the data to the Hill equation.Float
491Fit_R2-Replicate_14R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
492Fit_InfiniteActivity-Replicate_14The asymptotic efficacy from a fit of the data to the Hill equation.Float%
493Fit_ZeroActivity-Replicate_14Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
494Fit_CurveClass-Replicate_14Numerical encoding of curve description for the fitted Hill equation.Float
495Excluded_Points-Replicate_14Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
496Max_Response-Replicate_14Maximum activity observed for compound (usually at highest concentration tested).Float%
497Activity at 0.0005530000 uM-Replicate_14 (0.000553μM**)% Activity at given concentration.Float%
498Activity at 0.00276 uM-Replicate_14 (0.002765μM**)% Activity at given concentration.Float%
499Activity at 0.00765 uM-Replicate_14 (0.00764923μM**)% Activity at given concentration.Float%
500Activity at 0.012 uM-Replicate_14 (0.0124905μM**)% Activity at given concentration.Float%
501Activity at 0.024 uM-Replicate_14 (0.0241248μM**)% Activity at given concentration.Float%
502Activity at 0.034 uM-Replicate_14 (0.0339475μM**)% Activity at given concentration.Float%
503Activity at 0.074 uM-Replicate_14 (0.0737787μM**)% Activity at given concentration.Float%
504Activity at 0.148 uM-Replicate_14 (0.148068μM**)% Activity at given concentration.Float%
505Activity at 0.199 uM-Replicate_14 (0.199162μM**)% Activity at given concentration.Float%
506Activity at 0.370 uM-Replicate_14 (0.370495μM**)% Activity at given concentration.Float%
507Activity at 0.743 uM-Replicate_14 (0.743135μM**)% Activity at given concentration.Float%
508Activity at 1.207 uM-Replicate_14 (1.20721μM**)% Activity at given concentration.Float%
509Activity at 1.859 uM-Replicate_14 (1.8591μM**)% Activity at given concentration.Float%
510Activity at 3.722 uM-Replicate_14 (3.72239μM**)% Activity at given concentration.Float%
511Activity at 9.170 uM-Replicate_14 (9.16985μM**)% Activity at given concentration.Float%
512Activity at 13.42 uM-Replicate_14 (13.4244μM**)% Activity at given concentration.Float%
513Activity at 18.62 uM-Replicate_14 (18.6188μM**)% Activity at given concentration.Float%
514Activity at 45.97 uM-Replicate_14 (45.9725μM**)% Activity at given concentration.Float%
515Activity at 91.34 uM-Replicate_14 (91.3355μM**)% Activity at given concentration.Float%
516Activity at 95.67 uM-Replicate_14 (95.6717μM**)% Activity at given concentration.Float%
517Activity at 202.9 uM-Replicate_14 (202.913μM**)% Activity at given concentration.Float%
518Activity at 354.0 uM-Replicate_14 (354μM**)% Activity at given concentration.Float%
519Activity at 920.3 uM-Replicate_14 (920.3μM**)% Activity at given concentration.Float%
520Phenotype-Replicate_15Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive.String
521Potency-Replicate_15Concentration at which compound exhibits half-maximal efficacy, AC50. Extrapolated AC50s also include the highest efficacy observed and the concentration of compound at which it was observed.FloatμM
522Efficacy-Replicate_15Maximal efficacy of compound, reported as a percentage of control. These values are estimated based on fits of the Hill equation to the dose-response curves.Float%
523Analysis Comment-Replicate_15Annotation/notes on a particular compound's data or its analysis.String
524Activity_Score-Replicate_15Activity score.Integer
525Curve_Description-Replicate_15A description of dose-response curve quality. A complete curve has two observed asymptotes; a partial curve may not have attained its second asymptote at the highest concentration tested. High efficacy curves exhibit efficacy greater than 80% of control. Partial efficacies are statistically significant, but below 80% of control.String
526Fit_LogAC50-Replicate_15The logarithm of the AC50 from a fit of the data to the Hill equation (calculated based on Molar Units).Float
527Fit_HillSlope-Replicate_15The Hill slope from a fit of the data to the Hill equation.Float
528Fit_R2-Replicate_15R^2 fit value of the curve. Closer to 1.0 equates to better Hill equation fit.Float
529Fit_InfiniteActivity-Replicate_15The asymptotic efficacy from a fit of the data to the Hill equation.Float%
530Fit_ZeroActivity-Replicate_15Efficacy at zero concentration of compound from a fit of the data to the Hill equation.Float%
531Fit_CurveClass-Replicate_15Numerical encoding of curve description for the fitted Hill equation.Float
532Excluded_Points-Replicate_15Which dose-response titration points were excluded from analysis based on outlier analysis. Each number represents whether a titration point was (1) or was not (0) excluded, for the titration series going from smallest to highest compound concentrations.String
533Max_Response-Replicate_15Maximum activity observed for compound (usually at highest concentration tested).Float%
534Activity at 0.0005530000 uM-Replicate_15 (0.000553μM**)% Activity at given concentration.Float%
535Activity at 0.00276 uM-Replicate_15 (0.002765μM**)% Activity at given concentration.Float%
536Activity at 0.00765 uM-Replicate_15 (0.00764923μM**)% Activity at given concentration.Float%
537Activity at 0.012 uM-Replicate_15 (0.0124905μM**)% Activity at given concentration.Float%
538Activity at 0.024 uM-Replicate_15 (0.0241248μM**)% Activity at given concentration.Float%
539Activity at 0.034 uM-Replicate_15 (0.0339475μM**)% Activity at given concentration.Float%
540Activity at 0.074 uM-Replicate_15 (0.0737787μM**)% Activity at given concentration.Float%
541Activity at 0.148 uM-Replicate_15 (0.148068μM**)% Activity at given concentration.Float%
542Activity at 0.199 uM-Replicate_15 (0.199162μM**)% Activity at given concentration.Float%
543Activity at 0.370 uM-Replicate_15 (0.370495μM**)% Activity at given concentration.Float%
544Activity at 0.743 uM-Replicate_15 (0.743135μM**)% Activity at given concentration.Float%
545Activity at 1.207 uM-Replicate_15 (1.20721μM**)% Activity at given concentration.Float%
546Activity at 1.859 uM-Replicate_15 (1.8591μM**)% Activity at given concentration.Float%
547Activity at 3.722 uM-Replicate_15 (3.72239μM**)% Activity at given concentration.Float%
548Activity at 9.170 uM-Replicate_15 (9.16985μM**)% Activity at given concentration.Float%
549Activity at 13.42 uM-Replicate_15 (13.4244μM**)% Activity at given concentration.Float%
550Activity at 18.62 uM-Replicate_15 (18.6188μM**)% Activity at given concentration.Float%
551Activity at 45.97 uM-Replicate_15 (45.9725μM**)% Activity at given concentration.Float%
552Activity at 91.34 uM-Replicate_15 (91.3355μM**)% Activity at given concentration.Float%
553Activity at 95.67 uM-Replicate_15 (95.6717μM**)% Activity at given concentration.Float%
554Activity at 202.9 uM-Replicate_15 (202.913μM**)% Activity at given concentration.Float%
555Activity at 354.0 uM-Replicate_15 (354μM**)% Activity at given concentration.Float%
556Activity at 920.3 uM-Replicate_15 (920.3μM**)% Activity at given concentration.Float%

* Activity Concentration. ** Test Concentration.

Data Table (Concise)
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