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BioAssay: AID 682149

TP_TRANSPORTER: uptake in PGT-expressing HeLa cells

We recently identified a cDNA in the rat that encodes a broadly expressed PG transporter (PGT). Because PGs play diverse and important roles in human health and disease, we cloned human PGT (hPGT) from an adult human kidney cDNA library. A consensus sequence (4.0 kb) derived from several clones, plus 3' polymerase chain reaction amplification, exhibited 74% nucleic acid identity and 82% amino more ..
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 Tested Compounds
 Tested Compounds
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Unspecified(6)
 
 
 Tested Substances
 Tested Substances
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Unspecified(6)
 
 
 Related BioAssays
 Related BioAssays
AID: 682149
Data Source: ChEMBL (839288)
Depositor Category: Other
BioAssay Version:
Deposit Date: 2013-05-16
Modify Date: 2014-08-26

Data Table ( Complete ):           View All Data
Target
Sequence: RecName: Full=Solute carrier organic anion transporter family member 2A1; AltName: Full=Prostaglandin transporter; Short=PGT; AltName: Full=Solute carrier family 21 member 2
Description ..   
Protein Family: KAZAL_SLC21
Comment ..   

Gene:SLCO2A1     Related Protein 3D Structures     More BioActivity Data..
Tested Compounds:
Description:
Title: Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA(hPGT).

Abstract: We recently identified a cDNA in the rat that encodes a broadly expressed PG transporter (PGT). Because PGs play diverse and important roles in human health and disease, we cloned human PGT (hPGT) from an adult human kidney cDNA library. A consensus sequence (4.0 kb) derived from several clones, plus 3' polymerase chain reaction amplification, exhibited 74% nucleic acid identity and 82% amino acid identity compared to rat PGT. When transiently expressed in HeLa cells, a full-length clone catalyzed the transport of PGE1, PGE2, PGD2, PGF2alpha, and, to a lesser degree, TXB2. Northern blotting revealed mRNA transcripts of many different sizes in adult human heart, placenta, brain, lung, liver, skeletal muscle, pancreas, kidney, spleen, prostate, ovary, small intestine, and colon. hPGT mRNAs are also strongly expressed in human fetal brain, lung, liver, and kidney. The broad tissue distribution and substrate profile of hPGT suggest a role in the transport and/or metabolic clearance of PGs in diverse human tissues.
Categorized Comment - additional comments and annotations
From BioAssay Depositor:
Assay Type: Functional
Target Type: Target is a single protein chain
Assay Data Source: TP-search Transporter Database
BAO: Assay Format: cell-based format
Assay Cell Type: HeLa
Assay Test Type: In vitro
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
1Activity activity commentActivity activity commentString
2Activity standard flagActivity standard flagInteger
3Activity qualifierActivity qualifierString
4Activity published valueActivity published valueFloat
5Activity standard valueActivity standard valueFloat
6K1/2 activity commentK1/2 activity commentString
7K1/2 standard flagK1/2 standard flagInteger
8K1/2 qualifierK1/2 qualifierString
9K1/2 published valueK1/2 published valueFloatμM
10K1/2 standard valueK1/2 standard valueFloatμM

Data Table (Concise)
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