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BioAssay: AID 681342

TP_TRANSPORTER: uptake in Xenopus laevis oocytes

We have isolated a novel liver-specific organic anion transporter, LST-1, that is expressed exclusively in the human, rat, and mouse liver. LST-1 is a new gene family located between the organic anion transporter family and prostaglandin transporter. LST-1 transports taurocholate (Km = 13.6 microM) in a sodium-independent manner. LST-1 also shows broad substrate specificity. It transports more ..
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 Tested Compounds
 Tested Compounds
All(9)
 
 
Unspecified(9)
 
 
 Tested Substances
 Tested Substances
All(10)
 
 
Unspecified(10)
 
 
 Related BioAssays
 Related BioAssays
AID: 681342
Data Source: ChEMBL (838481)
Depositor Category: Other
BioAssay Version:
Deposit Date: 2013-05-16
Modify Date: 2013-11-17

Data Table ( Complete ):           All
Target
Sequence: RecName: Full=Solute carrier organic anion transporter family member 1B1; AltName: Full=Liver-specific organic anion transporter 1; Short=LST-1; AltName: Full=OATP-C; AltName: Full=Sodium-independent organic anion-transporting polypeptide 2; Short=OATP-2; AltName: Full=Solute carrier family 21 member 6
Description ..   
Protein Family: KAZAL_SLC21
Comment ..   

Gene:SLCO1B1     Related Protein 3D Structures     More BioActivity Data..
Tested Compounds:
Description:
Title: Identification of a novel gene family encoding human liver-specific organic anion transporter LST-1.

Abstract: We have isolated a novel liver-specific organic anion transporter, LST-1, that is expressed exclusively in the human, rat, and mouse liver. LST-1 is a new gene family located between the organic anion transporter family and prostaglandin transporter. LST-1 transports taurocholate (Km = 13.6 microM) in a sodium-independent manner. LST-1 also shows broad substrate specificity. It transports conjugated steroids (dehydroepiandrosterone sulfate, estradiol-17beta-glucuronide, and estrone-3-sulfate), eicosanoids (prostaglandin E2, thromboxane B2, leukotriene C4, leukotriene E4), and thyroid hormones (thyroxine, Km = 3.0 microM and triiodothyronine, Km = 2.7 microM), reflecting hepatic multispecificity. LST-1 is probably the most important transporter in human liver for clearance of bile acids and organic anions because hepatic levels of another organic anion transporter, OATP, is very low. This is also the first report of the human molecule that transports thyroid hormones.
Categorized Comment
ChEMBL Assay Type: Functional

ChEMBL Assay Data Source: TP-search Transporter Database

ChEMBL Assay Test Type: In vitro

ChEMBL Assay Cell Type: oocytes

ChEMBL Target ID: 103947

ChEMBL Target Type: Target is a single protein chain

Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
1Km*Km PubChem standard valueFloatμM
2Activity activity commentActivity activity commentString
3Activity standard flagActivity standard flagInteger
4Activity qualifierActivity qualifierString
5Activity published valueActivity published valueFloat
6Activity standard valueActivity standard valueFloat
7Km activity commentKm activity commentString
8Km standard flagKm standard flagInteger
9Km qualifierKm qualifierString
10Km published valueKm published valueFloatμM
11Km standard valueKm standard valueFloatnM

* Activity Concentration.

Data Table (Concise)
Classification
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