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BioAssay: AID 652280

NIH/3T3 (mouse embryonic fibroblast) toxicity Measured in Cell-Based System Using Plate Reader - 2138-02_Inhibitor_Dose_DryPowder_Activity_Set2

Assay Overview: The assay detects compounds that are cytotoxic to NIH3T3 cells afer ~90hrs in culture. After plating the NIH3T3 cells, compounds are added to the wells. After 90 hours culturing, all cells in the well are lysed and ATP is detected using Cell Titer Glo ..more
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 Tested Compounds
 Tested Compounds
All(244)
 
 
Active(20)
 
 
Inactive(166)
 
 
Inconclusive(61)
 
 
 Tested Substances
 Tested Substances
All(249)
 
 
Active(20)
 
 
Inactive(168)
 
 
Inconclusive(61)
 
 
 Related BioAssays
 Related BioAssays
AID: 652280
Data Source: Broad Institute (2138-02_Inhibitor_Dose_DryPowder_Activity_Set2)
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Depositor Category: NIH Molecular Libraries Probe Production Network
BioAssay Version:
Deposit Date: 2013-04-04
Hold-until Date: 2013-07-17
Modify Date: 2013-07-17

Data Table ( Complete ):           View Active Data    View All Data
BioActive Compounds: 20
Related Experiments
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AIDNameTypeProbeComment
624280Broad Institute Inhibition of T.cruzi replication in culture Inhibitor Probe ProjectSummary1 depositor-specified cross reference: Summary assay
624255Inhibition of T.cruzi proliferation in culture Measured in Cell-Based System Using Plate Reader - 2138-01_Inhibitor_SinglePoint_HTS_ActivityScreening same project related to Summary assay
651739Inhibition of T.cruzi proliferation in culture Measured in Cell-Based System Using Plate Reader - 2138-01_Inhibitor_SinglePoint_CherryPick_ActivityOther same project related to Summary assay
651740Inhibition of T.cruzi proliferation in culture Measured in Cell-Based System Using Plate Reader - 2138-01_Inhibitor_SinglePoint_CherryPick_Activity_Set2Other same project related to Summary assay
651742NIH/3T3 (mouse embryonic fibroblast) toxicity Measured in Cell-Based System Using Plate Reader - 2138-02_Inhibitor_SinglePoint_CherryPick_ActivityOther same project related to Summary assay
651744NIH/3T3 (mouse embryonic fibroblast) toxicity Measured in Cell-Based System Using Plate Reader - 2138-02_Inhibitor_SinglePoint_CherryPick_Activity_Set2Other same project related to Summary assay
651817NIH/3T3 (mouse embryonic fibroblast ) toxicity Measured in Cell-Based System Using Plate Reader - 2017-02_Inhibitor_Dose_CherryPick_Activity_Set2Confirmatory same project related to Summary assay
651818Intracellular Trypanosomes Measured in Cell-Based/Microorganism System Using Plate Reader - 2017-01_Inhibitor_Dose_CherryPick_Activity_Set3Confirmatory same project related to Summary assay
651844NIH/3T3 (mouse embryonic fibroblast) toxicity Measured in Cell-Based System Using Plate Reader - 2138-02_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651845Inhibition of T.cruzi proliferation in culture Measured in Cell-Based System Using Plate Reader - 2138-01_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651869Cidal vs. static assay: inhibition of T. cruzi (CAI/72) after prolonged treatment and treatment withdrawal Measured in Cell-Based System Using Imaging - 2138-08_Inhibitor_SinglePoint_DryPowder_Activity_Set2Other same project related to Summary assay
651877Cidal vs. static assay: inhibition of T. cruzi (CAI/72) after prolonged treatment and treatment withdrawal Measured in Cell-Based System Using Imaging - 2138-08_Inhibitor_SinglePoint_DryPowder_ActivityOther same project related to Summary assay
651881Inhibition of T. cruzi Tulahuen strain amastigotes in L-6 muscle cells Measured in Cell-Based and Microorganism Combination System Using Plate Reader - 2138-03_Inhibitor_Dose_CherryPick_ActivityConfirmatory same project related to Summary assay
651885Inhibition of intracellular CAI/72 strain of Trypanosoma cruzi in bovine embryo skeletal muscle cells: high content imaging Measured in Cell-Based System Using Imaging - 2138-07_Inhibitor_SinglePoint_DryPowder_ActivityConfirmatory same project related to Summary assay
651888Inhibition of Plasmodium falcipirum NF54 Measured in Cell-Based System Using Plate Reader - 2138-11_Inhibitor_Dose_CherryPick_ActivityConfirmatory same project related to Summary assay
651889Inhibition of Plasmodium falcipirum NF54 Measured in Cell-Based System Using Plate Reader - 2138-11_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651890Inhibition of T. cruzi Tulahuen strain amastigotes in L-6 muscle cells Measured in Cell-Based and Microorganism Combination System Using Plate Reader - 2138-03_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651891Inhibition of L. donovani axenic amastigotes Measured in Cell-Based System Using Plate Reader - 2138-04_Inhibitor_Dose_CherryPick_ActivityConfirmatory same project related to Summary assay
651892Inhibition of L. donovani axenic amastigotes Measured in Cell-Based System Using Plate Reader - 2138-04_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651893Inhibition of Trypanosoma brucei rhodesiense STIB900 Measured in Cell-Based System Using Plate Reader - 2138-05_Inhibitor_Dose_CherryPick_ActivityConfirmatory same project related to Summary assay
651894Inhibition of Trypanosoma brucei rhodesiense STIB900 Measured in Cell-Based System Using Plate Reader - 2138-05_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651895L-6 rat myocyte cell toxicity Measured in Cell-Based System Using Plate Reader - 2138-06_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651896L-6 rat myocyte cell toxicity Measured in Cell-Based System Using Plate Reader - 2138-06_Inhibitor_Dose_CherryPick_ActivityConfirmatory same project related to Summary assay
651897Inhibition of Trypanosoma cruzi Cruzain Measured in Biochemical System Using Plate Reader - 2138-09_Inhibitor_SinglePoint_DryPowder_ActivityConfirmatory same project related to Summary assay
651903Intracellular Trypanosomes Measured in Cell-Based/Microorganism System Using Plate Reader - 2017-01_Inhibitor_SinglePoint_HTS_Activity_Set2Screening same project related to Summary assay
652275Inhibition of T.cruzi proliferation in culture Measured in Cell-Based System Using Plate Reader - 2138-01_Inhibitor_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
652278Cidal vs. static assay: inhibition of T. cruzi (CAI/72) in J774 macrophages after prolonged compound treatment and treatment withdrawal Measured in Cell-Based System Using Imaging - 2138-13_Inhibitor_SinglePoint_DryPowder_Activity_Set2Other same project related to Summary assay
652279Cidal vs. static assay: inhibition of T. cruzi (CAI/72) in J774 macrophages after prolonged compound treatment and treatment withdrawal Measured in Cell-Based System Using Imaging - 2138-13_Inhibitor_SinglePoint_DryPowder_ActivityOther same project related to Summary assay
686919Inhibition of Trypanosoma cruzi Cruzain Measured in Biochemical System Using Plate Reader - 2138-09_Inhibitor_Dose_DryPowder_ActivityOther same project related to Summary assay
Description:
Keywords: NIH3T3, cytotoxic

Assay Overview: The assay detects compounds that are cytotoxic to NIH3T3 cells afer ~90hrs in culture. After plating the NIH3T3 cells, compounds are added to the wells. After 90 hours culturing, all cells in the well are lysed and ATP is detected using Cell Titer Glo

Expected Outcome: Compounds significantly suppressing luminescence, and therefore cytotoxic to NIH3T3s will be resolved as hits. This is a counter screen to primary screen to determine compounds that might yield false positive by killing host cell in T. cruzi invasion assays.
Protocol
PROTOCOL HTS ASSAY in 384-well plate
- NIH/3T3 cells (mouse embryonic fibroblast cell line; ATCC CRL-1658)
- DMEM with Phenol Red, high glucose, with L-glutamine and Sodium pyruvate (Cellgro, cat number: 10-013-CM)
- PSG or Penicillin-streptomycin-L-glutamine (Gibco-Invitrogen, cat number: 10378-016)
- FBS-Heat inactivated fetal bovine serum (Gibco-invitrogen, cat number: 16140-089)
- 0.25% Trypsin-EDTA 1X (Gibco-Invitrogen, cat number: 25200-072)
- Sterile PBS (Phosphate Buffer Saline) 1X
- Materials:
- T175, T225 culture flasks with vented caps (BD Falcon, cat number: 353028)
-Corning Hyperflasks (Corning, cat number: 10024)
Culture medium:
- For cell propagation:
90% DMEM+Phenol red, 10% FBS, 1% PSG. Mix and filter through 0.2 microns membrane. Keep at 4 degrees C. Warm up to 37 degrees C in water bath before use.
- For Tc culture and assays:
98% DMEM+Phenol red, 2% FBS, 1% PSG. Mix and filter through 0.2 microns membrane. Keep at 4 degrees C. Warm up to 37 degrees C in water bath before use.
Solutions:
-D. cell culture
NIH/3T3
Notes:
- NIH/3T3 cells are cultivated in DMEM supplemented with 10% FBS and 1% PSG in T175 or Corning Hyperflasks .
- Conditions can be adapted to other culture plate sizes.
Protocol:
- Aspirate medium.
- Rinse cells with 10 ml PBS/plate.
- Aspirate PBS.
- Add 4 ml of pre-warmed trypsin-EDTA, swirl the dish to make sure the trypsin covers all the cells.
- Leave the dish a few minutes at room temperature (usually not more than 5).
- Check that the cells are detaching.
- Add 21 ml of medium, pipet up and down to detach all the cells.
- Take 75 ul of media and add to Cellometer cassette (Nexcelom Biosciences). Using the Cellometer Auto T4 (Nexcelom Biosciences), focus so cell bodies have dark edges and clear/white centers. Select NIH3T3 preset menu, dilution 1x, display image, and count. Using this number dilute in either 2% FBS/DMEM for assays or 10% FBS/DMEM for propagation
E. Toxicity assay for HTS in 384 well plates:
Dilute to NIH/3T3 cells to 5000/60 ul of 2% FBS/DMEM media.
Plate 60 ul/well using Thermo MultiDrop Combi liquid dispenser and a sterilized standard sized dispensing cassette adding at a fast speed in a tissue culture hood.
Incubate cells 4-16 hrs at 37 C, 5%CO2.
Pin 100 nl compounds and incubate for an additional 90hrs.
Plates are equilibrated to room temperature for 1 hr.
30ul of diluted CellTiter-Glo (diluted 1:3 in PBS) is added to the well and incubated for 12 minutes.
Plates are read using the ultrasensistive luminescence program on the Envision (Perkin Elmer) 0.1s/well.
Comment
PRESENCE OF CONTROLS: Neutral control wells (NC; n=104) and positive control wells (PC; n=10) were included on every plate.
EXPECTED OUTCOME: Active compounds result in decreasing readout signal.
The compounds were assayed in multiple independent instances using an identical protocol; each instance is called a 'test'. For each test, the following analysis was applied:
ACTIVE CONCENTRATION LIMIT:
For each sample, the highest valid tested concentration (Max_Concentration) was determined and the active concentration limit (AC_limit) was set to equal (2)(Max_Concentration).
NORMALIZATION:
The raw signals of the plate wells were normalized using the 'Neutral Controls Minus Inhibitors' method in Genedata Assay Analyzer (v10.0.2):
The median raw signal of the intraplate neutral control wells was set to a normalized activity value of 0.
The median raw signal of the intraplate positive control wells was set to a normalized activity value of -100.
Experimental wells values were scaled to this range.
PATTERN CORRECTION: No plate pattern correction algorithm from Genedata Condoseo (v.10.0.2) was applied.
MEASUREMENT USED TO DETERMINE ACTIVE CONCENTRATION (AC): AC50
AC values were calculated using the curve fitting strategies in Genedata Screener Condoseo (7.0.3).
AC values were calculated up to the active concentration limit described for each sample.
pAC was set to equal -1*log10(AC)
PUBCHEM_ACTIVITY_OUTCOME:
Activity_Outcome = 1 (inactive) when:
a) compound shows activity but in a direction opposite to the expected outcome
in these cases, values describing curve fitting parameters (Sinf, S0, Hill Slope, log_AC50, log_AC50_SE) are set to null
b) curve fit is constant where activity is > -30% and < 30% at all tested concentrations, or
c) AC > AC_limit
Activity_Outcome = 2 (active) when:
AC <= AC_limit
Activity_Outcome = 3 (inconclusive) when:
a) Curve fitting strategy resulted in a constant fit with activity >= -70% but <= -30%, or
b) The fit was deemed not valid due to poor fit quality.
PUBCHEM_ACTIVITY_SCORE:
If PUBCHEM_ACTIVITY_OUTCOME = 1 (inactive) or 3 (inconclusive),
then PUBCHEM_ACTIVITY_SCORE = 0
If PUBCHEM_ACTIVITY_OUTCOME = 2 (active)
then PUBCHEM_ACTIVITY_SCORE = (10)(pAC)
Scores relate to AC in this manner:
120 = 1 pM
90 = 1 nM
60 = 1 uM
30 = 1 mM
0 = 1 M
When the active concentration (AC) is calculated to be greater than the highest valid tested concentration (Max_Concentration), the PUBCHEM_ACTIVITY_SCORE is calculated using Max_Concentration as the basis.
When the active concentration (AC) is calculated to be less than the lowest tested concentration, the PUBCHEM_ACTIVITY_SCORE is calculated using the lowest tested concentration as the basis.
DATA AGGREGATION:
Once the data for each test was processed, the test number was appended to all column headers in that test's data set. The individual test results were then aggregated as follows:
1. The final PUBCHEM_ACTIVITY_OUTCOME was set to:
a. '2' (active) when all test outcomes were '2' (active), or
b. '1' (inactive) when all test outcomes were '1' (inactive), or
c. '3' (inconclusive) when the test outcomes were mixed.
2. The final ACTIVE_CONCENTRATION (AC) was set as follows:
a. If the final PUBCHEM_ACTIVITY_OUTCOME = 2, AC was set as the mean of the constituent test active concentrations;
b. If the final PUBCHEM_ACTIVITY_OUTCOME = 1 or 3, AC was left empty.
3. The final PUBCHEM_ACTIVITY_SCORE was calculated based on the aggregated ACTIVE_CONCENTRATION, using the same logic described above for individual test scores.
Note:
The individual dose data point columns ('Activity_at_xxuM') reported here represent the median of valid (unmasked) replicate observations at each concentration. These values are the inputs to a curve fitting algorithm.
All other data columns represent values which are derived during the curve fitting algorithm; this may sometimes include automatic further masking of some replicate data points.
Occasionally this results in perceived inconsistencies: for example, between the derived 'Maximal_Activity' and the apparent most active data point.
Categorized Comment - additional comments and annotations
From PubChem:
Assay Format: Cell-based
Assay Type: Toxicity
Assay Cell Type: NIH 3T3
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1AC50_Qualifier'>','=', or '<'String
2AC50_uM*The concentration at which activity reaches 50% of the maximumFloatμM
3pAC50_MEqual to -1*log10(AC50)String
4ACTIVITY_OUTCOME_TEST1The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
5ACTIVITY_SCORE_TEST1The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
6ASSAYDATA_COMMENT_TEST1Comments relevant to the subset of data included in the indicated Test number.String
7AC50_Qualifier_TEST1'>','=', or '<'String
8AC50_uM_TEST1The concentration at which activity reaches 50% of the maximumFloatμM
9pAC50_M_TEST1Equal to -1*log10(AC50)String
10Hill_Slope_TEST1The slope at AC50Float
11S0_(%)_TEST1The fitted activity value at zero concentrationFloat%
12Sinf_(%)_TEST1The fitted activity value at infinite concentrationFloat%
13Num_Points_TEST1The number of data points used to generate the plotInteger
14Max_Activity_(%)_TEST1The maximum activity value observed, based on mean of replicates per concentrationFloat%
15Max_Activity_Conc_uM_TEST1The concentration at which the maximum activity is observedFloatμM
16Max_Concentration_uM_TEST1Maximum valid test concentrationFloatμM
17Activity_at_0uM_(%)_TEST1 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
18Activity_at_0.000001uM_(%)_TEST1 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
19Activity_at_0.000003uM_(%)_TEST1 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
20Activity_at_0.000010uM_(%)_TEST1 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
21Activity_at_0.000030uM_(%)_TEST1 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
22Activity_at_0.000090uM_(%)_TEST1 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
23Activity_at_0.000285uM_(%)_TEST1 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
24Activity_at_0.0008uM_(%)_TEST1 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
25Activity_at_0.0009uM_(%)_TEST1 (0.0009μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
26Activity_at_0.0026uM_(%)_TEST1 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
27Activity_at_0.0075uM_(%)_TEST1 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
28Activity_at_0.0235uM_(%)_TEST1 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
29Activity_at_0.068uM_(%)_TEST1 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
30Activity_at_0.21uM_(%)_TEST1 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
31Activity_at_0.6uM_(%)_TEST1 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
32Activity_at_1.8uM_(%)_TEST1 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
33Activity_at_5.6uM_(%)_TEST1 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
34Activity_at_16uM_(%)_TEST1 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
35ACTIVITY_OUTCOME_TEST2The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
36ACTIVITY_SCORE_TEST2The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
37ASSAYDATA_COMMENT_TEST2Comments relevant to the subset of data included in the indicated Test number.String
38AC50_Qualifier_TEST2'>','=', or '<'String
39AC50_uM_TEST2The concentration at which activity reaches 50% of the maximumFloatμM
40pAC50_M_TEST2Equal to -1*log10(AC50)String
41Hill_Slope_TEST2The slope at AC50Float
42S0_(%)_TEST2The fitted activity value at zero concentrationFloat%
43Sinf_(%)_TEST2The fitted activity value at infinite concentrationFloat%
44Num_Points_TEST2The number of data points used to generate the plotInteger
45Max_Activity_(%)_TEST2The maximum activity value observed, based on mean of replicates per concentrationFloat%
46Max_Activity_Conc_uM_TEST2The concentration at which the maximum activity is observedFloatμM
47Max_Concentration_uM_TEST2Maximum valid test concentrationFloatμM
48Activity_at_0uM_(%)_TEST2 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
49Activity_at_0.000001uM_(%)_TEST2 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
50Activity_at_0.000003uM_(%)_TEST2 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
51Activity_at_0.000010uM_(%)_TEST2 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
52Activity_at_0.000030uM_(%)_TEST2 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
53Activity_at_0.000090uM_(%)_TEST2 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
54Activity_at_0.000285uM_(%)_TEST2 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
55Activity_at_0.0008uM_(%)_TEST2 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
56Activity_at_0.0026uM_(%)_TEST2 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
57Activity_at_0.0075uM_(%)_TEST2 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
58Activity_at_0.0235uM_(%)_TEST2 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
59Activity_at_0.068uM_(%)_TEST2 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
60Activity_at_0.21uM_(%)_TEST2 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
61Activity_at_0.6uM_(%)_TEST2 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
62Activity_at_1.8uM_(%)_TEST2 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
63Activity_at_5.6uM_(%)_TEST2 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
64Activity_at_16uM_(%)_TEST2 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
65ACTIVITY_OUTCOME_TEST3The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
66ACTIVITY_SCORE_TEST3The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
67ASSAYDATA_COMMENT_TEST3Comments relevant to the subset of data included in the indicated Test number.String
68AC50_Qualifier_TEST3'>','=', or '<'String
69AC50_uM_TEST3The concentration at which activity reaches 50% of the maximumFloatμM
70pAC50_M_TEST3Equal to -1*log10(AC50)String
71Hill_Slope_TEST3The slope at AC50Float
72S0_(%)_TEST3The fitted activity value at zero concentrationFloat%
73Sinf_(%)_TEST3The fitted activity value at infinite concentrationFloat%
74Num_Points_TEST3The number of data points used to generate the plotInteger
75Max_Activity_(%)_TEST3The maximum activity value observed, based on mean of replicates per concentrationFloat%
76Max_Activity_Conc_uM_TEST3The concentration at which the maximum activity is observedFloatμM
77Max_Concentration_uM_TEST3Maximum valid test concentrationFloatμM
78Activity_at_0uM_(%)_TEST3 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
79Activity_at_0.000001uM_(%)_TEST3 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
80Activity_at_0.000003uM_(%)_TEST3 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
81Activity_at_0.000010uM_(%)_TEST3 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
82Activity_at_0.000030uM_(%)_TEST3 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
83Activity_at_0.000090uM_(%)_TEST3 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
84Activity_at_0.000285uM_(%)_TEST3 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
85Activity_at_0.0008uM_(%)_TEST3 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
86Activity_at_0.0026uM_(%)_TEST3 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
87Activity_at_0.0075uM_(%)_TEST3 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
88Activity_at_0.0235uM_(%)_TEST3 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
89Activity_at_0.068uM_(%)_TEST3 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
90Activity_at_0.21uM_(%)_TEST3 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
91Activity_at_0.6uM_(%)_TEST3 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
92Activity_at_1.8uM_(%)_TEST3 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
93Activity_at_5.6uM_(%)_TEST3 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
94Activity_at_16uM_(%)_TEST3 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
95ACTIVITY_OUTCOME_TEST4The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
96ACTIVITY_SCORE_TEST4The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
97ASSAYDATA_COMMENT_TEST4Comments relevant to the subset of data included in the indicated Test number.String
98AC50_Qualifier_TEST4'>','=', or '<'String
99AC50_uM_TEST4The concentration at which activity reaches 50% of the maximumFloatμM
100pAC50_M_TEST4Equal to -1*log10(AC50)String
101Hill_Slope_TEST4The slope at AC50Float
102S0_(%)_TEST4The fitted activity value at zero concentrationFloat%
103Sinf_(%)_TEST4The fitted activity value at infinite concentrationFloat%
104Num_Points_TEST4The number of data points used to generate the plotInteger
105Max_Activity_(%)_TEST4The maximum activity value observed, based on mean of replicates per concentrationFloat%
106Max_Activity_Conc_uM_TEST4The concentration at which the maximum activity is observedFloatμM
107Max_Concentration_uM_TEST4Maximum valid test concentrationFloatμM
108Activity_at_0uM_(%)_TEST4 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
109Activity_at_0.000001uM_(%)_TEST4 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
110Activity_at_0.000003uM_(%)_TEST4 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
111Activity_at_0.000010uM_(%)_TEST4 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
112Activity_at_0.000030uM_(%)_TEST4 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
113Activity_at_0.000090uM_(%)_TEST4 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
114Activity_at_0.000285uM_(%)_TEST4 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
115Activity_at_0.0008uM_(%)_TEST4 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
116Activity_at_0.0009uM_(%)_TEST4 (0.0009μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
117Activity_at_0.0026uM_(%)_TEST4 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
118Activity_at_0.0075uM_(%)_TEST4 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
119Activity_at_0.0235uM_(%)_TEST4 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
120Activity_at_0.068uM_(%)_TEST4 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
121Activity_at_0.21uM_(%)_TEST4 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
122Activity_at_0.6uM_(%)_TEST4 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
123Activity_at_1.8uM_(%)_TEST4 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
124Activity_at_5.6uM_(%)_TEST4 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
125Activity_at_16uM_(%)_TEST4 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
126ACTIVITY_OUTCOME_TEST5The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
127ACTIVITY_SCORE_TEST5The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
128ASSAYDATA_COMMENT_TEST5Comments relevant to the subset of data included in the indicated Test number.String
129AC50_Qualifier_TEST5'>','=', or '<'String
130AC50_uM_TEST5The concentration at which activity reaches 50% of the maximumFloatμM
131pAC50_M_TEST5Equal to -1*log10(AC50)String
132Hill_Slope_TEST5The slope at AC50Float
133S0_(%)_TEST5The fitted activity value at zero concentrationFloat%
134Sinf_(%)_TEST5The fitted activity value at infinite concentrationFloat%
135Num_Points_TEST5The number of data points used to generate the plotInteger
136Max_Activity_(%)_TEST5The maximum activity value observed, based on mean of replicates per concentrationFloat%
137Max_Activity_Conc_uM_TEST5The concentration at which the maximum activity is observedFloatμM
138Max_Concentration_uM_TEST5Maximum valid test concentrationFloatμM
139Activity_at_0uM_(%)_TEST5 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
140Activity_at_0.000001uM_(%)_TEST5 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
141Activity_at_0.000003uM_(%)_TEST5 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
142Activity_at_0.000010uM_(%)_TEST5 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
143Activity_at_0.000030uM_(%)_TEST5 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
144Activity_at_0.000090uM_(%)_TEST5 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
145Activity_at_0.000285uM_(%)_TEST5 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
146Activity_at_0.0008uM_(%)_TEST5 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
147Activity_at_0.0009uM_(%)_TEST5 (0.0009μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
148Activity_at_0.0026uM_(%)_TEST5 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
149Activity_at_0.0075uM_(%)_TEST5 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
150Activity_at_0.0235uM_(%)_TEST5 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
151Activity_at_0.068uM_(%)_TEST5 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
152Activity_at_0.21uM_(%)_TEST5 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
153Activity_at_0.6uM_(%)_TEST5 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
154Activity_at_1.8uM_(%)_TEST5 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
155Activity_at_5.6uM_(%)_TEST5 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
156Activity_at_16uM_(%)_TEST5 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
157ACTIVITY_OUTCOME_TEST6The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
158ACTIVITY_SCORE_TEST6The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
159ASSAYDATA_COMMENT_TEST6Comments relevant to the subset of data included in the indicated Test number.String
160AC50_Qualifier_TEST6'>','=', or '<'String
161AC50_uM_TEST6The concentration at which activity reaches 50% of the maximumFloatμM
162pAC50_M_TEST6Equal to -1*log10(AC50)String
163Hill_Slope_TEST6The slope at AC50Float
164S0_(%)_TEST6The fitted activity value at zero concentrationFloat%
165Sinf_(%)_TEST6The fitted activity value at infinite concentrationFloat%
166Num_Points_TEST6The number of data points used to generate the plotInteger
167Max_Activity_(%)_TEST6The maximum activity value observed, based on mean of replicates per concentrationFloat%
168Max_Activity_Conc_uM_TEST6The concentration at which the maximum activity is observedFloatμM
169Max_Concentration_uM_TEST6Maximum valid test concentrationFloatμM
170Activity_at_0uM_(%)_TEST6 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
171Activity_at_0.000001uM_(%)_TEST6 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
172Activity_at_0.000003uM_(%)_TEST6 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
173Activity_at_0.000010uM_(%)_TEST6 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
174Activity_at_0.000030uM_(%)_TEST6 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
175Activity_at_0.000090uM_(%)_TEST6 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
176Activity_at_0.000285uM_(%)_TEST6 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
177Activity_at_0.0008uM_(%)_TEST6 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
178Activity_at_0.0026uM_(%)_TEST6 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
179Activity_at_0.0075uM_(%)_TEST6 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
180Activity_at_0.0235uM_(%)_TEST6 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
181Activity_at_0.068uM_(%)_TEST6 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
182Activity_at_0.21uM_(%)_TEST6 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
183Activity_at_0.6uM_(%)_TEST6 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
184Activity_at_1.8uM_(%)_TEST6 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
185Activity_at_5.6uM_(%)_TEST6 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
186Activity_at_16uM_(%)_TEST6 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
187ACTIVITY_OUTCOME_TEST7The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
188ACTIVITY_SCORE_TEST7The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
189ASSAYDATA_COMMENT_TEST7Comments relevant to the subset of data included in the indicated Test number.String
190AC50_Qualifier_TEST7'>','=', or '<'String
191AC50_uM_TEST7The concentration at which activity reaches 50% of the maximumFloatμM
192pAC50_M_TEST7Equal to -1*log10(AC50)String
193Hill_Slope_TEST7The slope at AC50Float
194S0_(%)_TEST7The fitted activity value at zero concentrationFloat%
195Sinf_(%)_TEST7The fitted activity value at infinite concentrationFloat%
196Num_Points_TEST7The number of data points used to generate the plotInteger
197Max_Activity_(%)_TEST7The maximum activity value observed, based on mean of replicates per concentrationFloat%
198Max_Activity_Conc_uM_TEST7The concentration at which the maximum activity is observedFloatμM
199Max_Concentration_uM_TEST7Maximum valid test concentrationFloatμM
200Activity_at_0uM_(%)_TEST7 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
201Activity_at_0.000001uM_(%)_TEST7 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
202Activity_at_0.000003uM_(%)_TEST7 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
203Activity_at_0.000010uM_(%)_TEST7 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
204Activity_at_0.000030uM_(%)_TEST7 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
205Activity_at_0.000090uM_(%)_TEST7 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
206Activity_at_0.000285uM_(%)_TEST7 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
207Activity_at_0.0008uM_(%)_TEST7 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
208Activity_at_0.0026uM_(%)_TEST7 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
209Activity_at_0.0075uM_(%)_TEST7 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
210Activity_at_0.0235uM_(%)_TEST7 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
211Activity_at_0.068uM_(%)_TEST7 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
212Activity_at_0.21uM_(%)_TEST7 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
213Activity_at_0.6uM_(%)_TEST7 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
214Activity_at_1.8uM_(%)_TEST7 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
215Activity_at_5.6uM_(%)_TEST7 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
216Activity_at_16uM_(%)_TEST7 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
217ACTIVITY_OUTCOME_TEST8The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
218ACTIVITY_SCORE_TEST8The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
219ASSAYDATA_COMMENT_TEST8Comments relevant to the subset of data included in the indicated Test number.String
220AC50_Qualifier_TEST8'>','=', or '<'String
221AC50_uM_TEST8The concentration at which activity reaches 50% of the maximumFloatμM
222pAC50_M_TEST8Equal to -1*log10(AC50)String
223Hill_Slope_TEST8The slope at AC50Float
224S0_(%)_TEST8The fitted activity value at zero concentrationFloat%
225Sinf_(%)_TEST8The fitted activity value at infinite concentrationFloat%
226Num_Points_TEST8The number of data points used to generate the plotInteger
227Max_Activity_(%)_TEST8The maximum activity value observed, based on mean of replicates per concentrationFloat%
228Max_Activity_Conc_uM_TEST8The concentration at which the maximum activity is observedFloatμM
229Max_Concentration_uM_TEST8Maximum valid test concentrationFloatμM
230Activity_at_0uM_(%)_TEST8 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
231Activity_at_0.000001uM_(%)_TEST8 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
232Activity_at_0.000003uM_(%)_TEST8 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
233Activity_at_0.000010uM_(%)_TEST8 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
234Activity_at_0.000030uM_(%)_TEST8 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
235Activity_at_0.000090uM_(%)_TEST8 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
236Activity_at_0.000285uM_(%)_TEST8 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
237Activity_at_0.0008uM_(%)_TEST8 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
238Activity_at_0.0026uM_(%)_TEST8 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
239Activity_at_0.0075uM_(%)_TEST8 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
240Activity_at_0.0235uM_(%)_TEST8 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
241Activity_at_0.068uM_(%)_TEST8 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
242Activity_at_0.21uM_(%)_TEST8 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
243Activity_at_0.6uM_(%)_TEST8 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
244Activity_at_1.8uM_(%)_TEST8 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
245Activity_at_5.6uM_(%)_TEST8 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
246Activity_at_16uM_(%)_TEST8 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
247ACTIVITY_OUTCOME_TEST9The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
248ACTIVITY_SCORE_TEST9The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
249ASSAYDATA_COMMENT_TEST9Comments relevant to the subset of data included in the indicated Test number.String
250AC50_Qualifier_TEST9'>','=', or '<'String
251AC50_uM_TEST9The concentration at which activity reaches 50% of the maximumFloatμM
252pAC50_M_TEST9Equal to -1*log10(AC50)String
253Hill_Slope_TEST9The slope at AC50Float
254S0_(%)_TEST9The fitted activity value at zero concentrationFloat%
255Sinf_(%)_TEST9The fitted activity value at infinite concentrationFloat%
256Num_Points_TEST9The number of data points used to generate the plotInteger
257Max_Activity_(%)_TEST9The maximum activity value observed, based on mean of replicates per concentrationFloat%
258Max_Activity_Conc_uM_TEST9The concentration at which the maximum activity is observedFloatμM
259Max_Concentration_uM_TEST9Maximum valid test concentrationFloatμM
260Activity_at_0uM_(%)_TEST9 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
261Activity_at_0.000001uM_(%)_TEST9 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
262Activity_at_0.000003uM_(%)_TEST9 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
263Activity_at_0.000010uM_(%)_TEST9 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
264Activity_at_0.000030uM_(%)_TEST9 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
265Activity_at_0.000090uM_(%)_TEST9 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
266Activity_at_0.000285uM_(%)_TEST9 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
267Activity_at_0.0008uM_(%)_TEST9 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
268Activity_at_0.0026uM_(%)_TEST9 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
269Activity_at_0.0075uM_(%)_TEST9 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
270Activity_at_0.0235uM_(%)_TEST9 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
271Activity_at_0.068uM_(%)_TEST9 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
272Activity_at_0.21uM_(%)_TEST9 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
273Activity_at_0.6uM_(%)_TEST9 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
274Activity_at_1.8uM_(%)_TEST9 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
275Activity_at_5.6uM_(%)_TEST9 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
276Activity_at_16uM_(%)_TEST9 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
277ACTIVITY_OUTCOME_TEST10The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
278ACTIVITY_SCORE_TEST10The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
279ASSAYDATA_COMMENT_TEST10Comments relevant to the subset of data included in the indicated Test number.String
280AC50_Qualifier_TEST10'>','=', or '<'String
281AC50_uM_TEST10The concentration at which activity reaches 50% of the maximumFloatμM
282pAC50_M_TEST10Equal to -1*log10(AC50)String
283Hill_Slope_TEST10The slope at AC50Float
284S0_(%)_TEST10The fitted activity value at zero concentrationFloat%
285Sinf_(%)_TEST10The fitted activity value at infinite concentrationFloat%
286Num_Points_TEST10The number of data points used to generate the plotInteger
287Max_Activity_(%)_TEST10The maximum activity value observed, based on mean of replicates per concentrationFloat%
288Max_Activity_Conc_uM_TEST10The concentration at which the maximum activity is observedFloatμM
289Max_Concentration_uM_TEST10Maximum valid test concentrationFloatμM
290Activity_at_0uM_(%)_TEST10 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
291Activity_at_0.000001uM_(%)_TEST10 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
292Activity_at_0.000003uM_(%)_TEST10 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
293Activity_at_0.000010uM_(%)_TEST10 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
294Activity_at_0.000030uM_(%)_TEST10 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
295Activity_at_0.000090uM_(%)_TEST10 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
296Activity_at_0.000285uM_(%)_TEST10 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
297Activity_at_0.0008uM_(%)_TEST10 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
298Activity_at_0.0026uM_(%)_TEST10 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
299Activity_at_0.0075uM_(%)_TEST10 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
300Activity_at_0.0235uM_(%)_TEST10 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
301Activity_at_0.068uM_(%)_TEST10 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
302Activity_at_0.21uM_(%)_TEST10 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
303Activity_at_0.6uM_(%)_TEST10 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
304Activity_at_1.8uM_(%)_TEST10 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
305Activity_at_5.6uM_(%)_TEST10 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
306Activity_at_16uM_(%)_TEST10 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
307ACTIVITY_OUTCOME_TEST11The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
308ACTIVITY_SCORE_TEST11The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
309ASSAYDATA_COMMENT_TEST11Comments relevant to the subset of data included in the indicated Test number.String
310AC50_Qualifier_TEST11'>','=', or '<'String
311AC50_uM_TEST11The concentration at which activity reaches 50% of the maximumFloatμM
312pAC50_M_TEST11Equal to -1*log10(AC50)String
313Hill_Slope_TEST11The slope at AC50Float
314S0_(%)_TEST11The fitted activity value at zero concentrationFloat%
315Sinf_(%)_TEST11The fitted activity value at infinite concentrationFloat%
316Num_Points_TEST11The number of data points used to generate the plotInteger
317Max_Activity_(%)_TEST11The maximum activity value observed, based on mean of replicates per concentrationFloat%
318Max_Activity_Conc_uM_TEST11The concentration at which the maximum activity is observedFloatμM
319Max_Concentration_uM_TEST11Maximum valid test concentrationFloatμM
320Activity_at_0uM_(%)_TEST11 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
321Activity_at_0.000001uM_(%)_TEST11 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
322Activity_at_0.000003uM_(%)_TEST11 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
323Activity_at_0.000010uM_(%)_TEST11 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
324Activity_at_0.000030uM_(%)_TEST11 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
325Activity_at_0.000090uM_(%)_TEST11 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
326Activity_at_0.000285uM_(%)_TEST11 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
327Activity_at_0.0008uM_(%)_TEST11 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
328Activity_at_0.0026uM_(%)_TEST11 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
329Activity_at_0.0075uM_(%)_TEST11 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
330Activity_at_0.0235uM_(%)_TEST11 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
331Activity_at_0.068uM_(%)_TEST11 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
332Activity_at_0.21uM_(%)_TEST11 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
333Activity_at_0.6uM_(%)_TEST11 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
334Activity_at_1.8uM_(%)_TEST11 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
335Activity_at_5.6uM_(%)_TEST11 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
336Activity_at_16uM_(%)_TEST11 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
337ACTIVITY_OUTCOME_TEST12The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
338ACTIVITY_SCORE_TEST12The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
339ASSAYDATA_COMMENT_TEST12Comments relevant to the subset of data included in the indicated Test number.String
340AC50_Qualifier_TEST12'>','=', or '<'String
341AC50_uM_TEST12The concentration at which activity reaches 50% of the maximumFloatμM
342pAC50_M_TEST12Equal to -1*log10(AC50)String
343Hill_Slope_TEST12The slope at AC50Float
344S0_(%)_TEST12The fitted activity value at zero concentrationFloat%
345Sinf_(%)_TEST12The fitted activity value at infinite concentrationFloat%
346Num_Points_TEST12The number of data points used to generate the plotInteger
347Max_Activity_(%)_TEST12The maximum activity value observed, based on mean of replicates per concentrationFloat%
348Max_Activity_Conc_uM_TEST12The concentration at which the maximum activity is observedFloatμM
349Max_Concentration_uM_TEST12Maximum valid test concentrationFloatμM
350Activity_at_0uM_(%)_TEST12 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
351Activity_at_0.000001uM_(%)_TEST12 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
352Activity_at_0.000003uM_(%)_TEST12 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
353Activity_at_0.000009uM_(%)_TEST12 (9e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
354Activity_at_0.000010uM_(%)_TEST12 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
355Activity_at_0.000011uM_(%)_TEST12 (1.1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
356Activity_at_0.000030uM_(%)_TEST12 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
357Activity_at_0.000056uM_(%)_TEST12 (5.6e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
358Activity_at_0.000060uM_(%)_TEST12 (6e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
359Activity_at_0.000068uM_(%)_TEST12 (6.8e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
360Activity_at_0.000090uM_(%)_TEST12 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
361Activity_at_0.0001uM_(%)_TEST12 (0.0001μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
362Activity_at_0.000285uM_(%)_TEST12 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
363Activity_at_0.00035uM_(%)_TEST12 (0.00035μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
364Activity_at_0.00038uM_(%)_TEST12 (0.00038μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
365Activity_at_0.0008uM_(%)_TEST12 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
366Activity_at_0.0009uM_(%)_TEST12 (0.0009μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
367Activity_at_0.00195uM_(%)_TEST12 (0.00195μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
368Activity_at_0.0021uM_(%)_TEST12 (0.0021μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
369Activity_at_0.00235uM_(%)_TEST12 (0.00235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
370Activity_at_0.0026uM_(%)_TEST12 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
371Activity_at_0.0075uM_(%)_TEST12 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
372Activity_at_0.008uM_(%)_TEST12 (0.008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
373Activity_at_0.012uM_(%)_TEST12 (0.012μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
374Activity_at_0.0135uM_(%)_TEST12 (0.0135μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
375Activity_at_0.0235uM_(%)_TEST12 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
376Activity_at_0.068uM_(%)_TEST12 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
377Activity_at_0.075uM_(%)_TEST12 (0.075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
378Activity_at_0.08uM_(%)_TEST12 (0.08μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
379Activity_at_0.21uM_(%)_TEST12 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
380Activity_at_0.42uM_(%)_TEST12 (0.42μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
381Activity_at_0.46uM_(%)_TEST12 (0.46μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
382Activity_at_0.5uM_(%)_TEST12 (0.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
383Activity_at_0.6uM_(%)_TEST12 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
384Activity_at_1.8uM_(%)_TEST12 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
385Activity_at_1.95uM_(%)_TEST12 (1.95μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
386Activity_at_2.6uM_(%)_TEST12 (2.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
387Activity_at_2.85uM_(%)_TEST12 (2.85μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
388Activity_at_3uM_(%)_TEST12 (3μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
389Activity_at_5.6uM_(%)_TEST12 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
390Activity_at_16uM_(%)_TEST12 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
391Activity_at_18uM_(%)_TEST12 (18μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
392ACTIVITY_OUTCOME_TEST13The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
393ACTIVITY_SCORE_TEST13The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
394ASSAYDATA_COMMENT_TEST13Comments relevant to the subset of data included in the indicated Test number.String
395AC50_Qualifier_TEST13'>','=', or '<'String
396AC50_uM_TEST13The concentration at which activity reaches 50% of the maximumFloatμM
397pAC50_M_TEST13Equal to -1*log10(AC50)String
398Hill_Slope_TEST13The slope at AC50Float
399S0_(%)_TEST13The fitted activity value at zero concentrationFloat%
400Sinf_(%)_TEST13The fitted activity value at infinite concentrationFloat%
401Num_Points_TEST13The number of data points used to generate the plotInteger
402Max_Activity_(%)_TEST13The maximum activity value observed, based on mean of replicates per concentrationFloat%
403Max_Activity_Conc_uM_TEST13The concentration at which the maximum activity is observedFloatμM
404Max_Concentration_uM_TEST13Maximum valid test concentrationFloatμM
405Activity_at_0uM_(%)_TEST13 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
406Activity_at_0.000001uM_(%)_TEST13 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
407Activity_at_0.000003uM_(%)_TEST13 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
408Activity_at_0.000009uM_(%)_TEST13 (9e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
409Activity_at_0.000010uM_(%)_TEST13 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
410Activity_at_0.000011uM_(%)_TEST13 (1.1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
411Activity_at_0.000030uM_(%)_TEST13 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
412Activity_at_0.000056uM_(%)_TEST13 (5.6e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
413Activity_at_0.000060uM_(%)_TEST13 (6e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
414Activity_at_0.000068uM_(%)_TEST13 (6.8e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
415Activity_at_0.000090uM_(%)_TEST13 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
416Activity_at_0.0001uM_(%)_TEST13 (0.0001μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
417Activity_at_0.000285uM_(%)_TEST13 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
418Activity_at_0.00035uM_(%)_TEST13 (0.00035μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
419Activity_at_0.00038uM_(%)_TEST13 (0.00038μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
420Activity_at_0.0008uM_(%)_TEST13 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
421Activity_at_0.0009uM_(%)_TEST13 (0.0009μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
422Activity_at_0.00195uM_(%)_TEST13 (0.00195μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
423Activity_at_0.0021uM_(%)_TEST13 (0.0021μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
424Activity_at_0.00235uM_(%)_TEST13 (0.00235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
425Activity_at_0.0026uM_(%)_TEST13 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
426Activity_at_0.0075uM_(%)_TEST13 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
427Activity_at_0.008uM_(%)_TEST13 (0.008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
428Activity_at_0.012uM_(%)_TEST13 (0.012μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
429Activity_at_0.0135uM_(%)_TEST13 (0.0135μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
430Activity_at_0.0235uM_(%)_TEST13 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
431Activity_at_0.068uM_(%)_TEST13 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
432Activity_at_0.075uM_(%)_TEST13 (0.075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
433Activity_at_0.08uM_(%)_TEST13 (0.08μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
434Activity_at_0.21uM_(%)_TEST13 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
435Activity_at_0.42uM_(%)_TEST13 (0.42μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
436Activity_at_0.46uM_(%)_TEST13 (0.46μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
437Activity_at_0.5uM_(%)_TEST13 (0.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
438Activity_at_0.6uM_(%)_TEST13 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
439Activity_at_1.8uM_(%)_TEST13 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
440Activity_at_1.95uM_(%)_TEST13 (1.95μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
441Activity_at_2.6uM_(%)_TEST13 (2.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
442Activity_at_2.85uM_(%)_TEST13 (2.85μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
443Activity_at_3uM_(%)_TEST13 (3μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
444Activity_at_5.6uM_(%)_TEST13 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
445Activity_at_16uM_(%)_TEST13 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
446Activity_at_18uM_(%)_TEST13 (18μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%

* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: 1 R03 MH085673-01

Data Table (Concise)
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