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BioAssay: AID 651992

Late-stage results from the probe development effort to identify transcriptional activators of heat shock protein 70 (Hsp70): yellow fluorescent protein (YFP) quenching assay to assess changes in trafficking of DeltaF508-CFTR

Name: Late-stage results from the probe development effort to identify transcriptional activators of heat shock protein 70 (Hsp70): yellow fluorescent protein (YFP) quenching assay to assess changes in trafficking of DeltaF508-CFTR. ..more
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AID: 651992
Data Source: The Scripps Research Institute Molecular Screening Center (DeltaF508-CFTR_ACT_FLUO)
Depositor Category: NIH Molecular Libraries Screening Center Network
Deposit Date: 2013-01-23

Data Table ( Complete ):           View Active Data    View All Data
Target
BioActive Compound: 1
Related Experiments
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AIDNameTypeComment
1203Primary cell-based high-throughput screening assay to identify transcriptional activators of heat shock protein 70 (Hsp70)Screeningdepositor-specified cross reference: Primary screen (HSP70 activators in triplicate)
1252Dose response cell-based high-throughput screening assay to identify transcriptional activators of heat shock protein 70 (Hsp70)Confirmatorydepositor-specified cross reference: Dose response (HSP70 activators in triplicate)
1259Cytotoxicity counterscreen assay for transcriptional activators of heat shock protein 70 (Hsp70)Confirmatorydepositor-specified cross reference: Dose response (Cytotoxicity in triplicate)
588815Summary of the probe development efforts to identify transcriptional activators of heat shock protein 70 (Hsp70).Summarydepositor-specified cross reference: Summary (HSP70 activators)
651945Late-stage results from the probe development effort to identify transcriptional activators of heat shock protein 70 (Hsp70): fluorescence-based biochemical quantitative polymerase chain reaction assay to assess Hsp70 gene expression changesOtherdepositor-specified cross reference: Late-stage assay (Hsp70 gene expression changes)
651948Late-stage results from the probe development effort to identify transcriptional activators of heat shock protein 70 (Hsp70): chemiluminescence-based biochemical western blot assay for transcriptional activators of heat shock protein Hsp70Otherdepositor-specified cross reference: Late-stage assay (Hsp70 transcriptional activators)
651950Late stage assay for the probe development effort to identify transcriptional activators of heat shock protein 70 (Hsp70). Luminescence-based-cell-based high-throughput dose response assay for Hsp70 activatorsOtherdepositor-specified cross reference: Late-stage dose response (Hsp70 activators)
651963Late-stage results from the probe development effort to identify transcriptional activators of heat shock protein 70 (Hsp70): fluorescence-based cell-based microscopic assay to assess aggregation of polyQ35 in C. elegans larvaeOtherdepositor-specified cross reference: Late-stage assay (aggregation of polyQ35 in C. elegans larvae)
651964Late-stage results from the probe development effort to identify transcriptional activators of heat shock protein 70 (Hsp70): fluorescence-based biochemical quantitative polymerase chain reaction assay to assess gene expression changes in downstream target genesOtherdepositor-specified cross reference: Late-stage assay (gene expression changes in downstream target genes)
Description:
Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center
Center Affiliation: The Scripps Research Institute (TSRI)
Assay Provider: Richard Morimoto, Northwestern University
Network: Molecular Library Screening Center Network (MLSCN)
Grant Proposal Number: 5 R21 NS056337-02
Grant Proposal PI: Richard Morimoto

External Assay ID: DeltaF508-CFTR_ACT_FLUO

Name: Late-stage results from the probe development effort to identify transcriptional activators of heat shock protein 70 (Hsp70): yellow fluorescent protein (YFP) quenching assay to assess changes in trafficking of DeltaF508-CFTR.

Description:

The human heat shock protein 70 (Hsp70) family is evolutionarily conserved among all organisms from archaebacteria to humans, suggesting an essential role in cell survival (1, 2). Under circumstances of transient cell stress, the heat shock response and activities of molecular chaperones can restore protein homeostasis. In human disease, however, misfolded proteins can accumulate when polyglutamine-expansion proteins are chronically expressed over the life of the cell. Elevated expression of molecular chaperones suppresses protein misfolding/aggregation and toxicity phenotypes in various model systems of Huntington's disease, Alzheimer's disease, Parkinson's disease, and Amyotrophic Lateral Sclerosis (ALS). Mutations in the respective proteins huntingtin, tau, alpha-synuclein, and superoxide dismutase (SOD1), associated with these diseases, result in the appearance of misfolded species that adopt alternate conformations. These observations led to the proposal that a common feature of diverse diseases of protein conformation is the appearance of alternate folded states that self-associate and form toxic species and protein aggregates.

A role for Hsp70 family proteins in controlling these events has been widely studied. Studies with mammalian tissue culture cells, transgenic mice, Drosophila, and C. elegans have established that the heat shock response can be activated in cells expressing aggregation-prone proteins, suggesting a role for molecular chaperones as an adaptive survival response (3, 4). Moreover, a direct relationship with polyglutamine diseases is suggested by the co-localization of several heat shock proteins, including Hdj-1, Hdj-2, Hsp70 and ubiquitin with polyglutamine aggregates in the tissues of affected individuals, transgenic mice and tissue culture cells (5). Finally, overexpression of Hsp70 can suppress the toxicity associated with the accumulation of misfolded proteins (6-8). High throughput screening initiatives aimed at the identification of compounds that enhance the heat shock response, in particular Hsp70, will provide insights into this conserved cellular process and may lead to novel therapeutics for these devastating disorders.


References:

1.Gupta, R.S., and Singh, B. 1994. Phylogenetic analysis of 70 kD heat shock protein sequences suggests a chimeric origin for the eukaryotic cell nucleus. Curr Biol 4:1104-1114.
2.Lindquist, S., and Craig, E.A. 1988. The heat-shock proteins. Annu Rev Genet 22:631-677.
3.Satyal, S.H., Schmidt, E., Kitagawa, K., Sondheimer, N., Lindquist, S., Kramer, J.M., and Morimoto, R.I. 2000. Polyglutamine aggregates alter protein folding homeostasis in Caenorhabditis elegans. Proc Natl Acad Sci U S A 97:5750-5755.
4.Wyttenbach, A., Carmichael, J., Swartz, J., Furlong, R.A., Narain, Y., Rankin, J., and Rubinsztein, D.C. 2000. Effects of heat shock, heat shock protein 40 (HDJ-2), and proteasome inhibition on protein aggregation in cellular models of Huntington's disease. Proc Natl Acad Sci U S A 97:2898-2903.
5.Cummings, C.J., Mancini, M.A., Antalffy, B., DeFranco, D.B., Orr, H.T., and Zoghbi, H.Y. 1998. Chaperone suppression of aggregation and altered subcellular proteasome localization imply protein misfolding in SCA1. Nat Genet 19:148-154.
6.Krobitsch, S., and Lindquist, S. 2000. Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins. Proc Natl Acad Sci U S A 97:1589-1594.
7.Kazemi-Esfarjani, P., and Benzer, S. 2000. Genetic suppression of polyglutamine toxicity in Drosophila. Science 287:1837-1840.
8.Warrick, J.M., Chan, H.Y., Gray-Board, G.L., Chai, Y., Paulson, H.L., and Bonini, N.M. 1999. Suppression of polyglutamine-mediated neurodegeneration in Drosophila by the molecular chaperone HSP70. Nat Genet 23:425-428.

Keywords:

Hsp70, HSPA1A, HSF1, heat shock transcription factor 1, chaperone, agonist, activator, cystic fibrosis, human bronchial epithelial cell line, epithelial cell line, cystic fibrosis transmembrane conductance regulator, CFTR, yellow fluorescent protein, YFP, halide, trafficking, proteostasis, proteostasis regulator, counterscreen, fluorescence, late stage, late stage AID, assay provider, powders, Scripps, Scripps Research Institute Molecular Screening Center, Molecular Library Screening Center Network, MLSCN.
Protocol
Assay Overview:

The purpose of this assay is to determine whether a powder sample of a compound identified as a transcriptional activator of heat shock protein 70 (Hsp70) is active in the correction of defective trafficking in a cellular model of cystic fibrosis. This model corresponds to a human bronchial epithelial cell line (CFBE41o-) stably co-expressing the DeltaF508 alteration of the cystic fibrosis transmembrane conductance regulator (DeltaF508-CFTR) and a halide-sensing mutant of yellow fluorescent protein (YFPH148Q I152L). DeltaF508-CFTR is defective both in trafficking from the endoplasmic reticulum to the plasma membrane and in channel gating. As designed, correction of the defective trafficking of DeltaF508-CFTR with the proteostasis regulators would lead to an increased amount of active protein at the cell surface, allowing an increased flow of extracellular halides into the cell, which would result in a reduction, or quenching, of YFP fluorescence intensity.

Protocol Summary:

Human bronchial epithelial cells (CFBE41o-) stably expressing DeltaF508-CFTR as well as H148Q/I152L-YFP (CFBE41o- -YFP) were added to a 96-well black walled plate and grown to confluency in growth media (alpha-MEM containing 100 U/ml penicillin, 100 U/ml streptomycin, 10% v/v FBS, 2 mM L-glutamine, 2 ug/ml puromycin and 0.75 ug/ml G418). Cells were treated with the indicated concentration of compounds in complete growth media and incubated at 37 C, 5% CO2 for 24 hours. Cells were subsequently washed three times with 200 uL of PBS pH 7.4 (137 mM NaCl, 2.7 mM KCl, 0.7 mM CaCl2, 1.1 mM MgCl2, 1.5 mM KH2PO4, 8.1 mM Na2HPO4) and equilibrated in 40 uL of PBS pH 7.4 and maintained at 37 C throughout. Cells were stimulated with a final concentration of 10 uM forskolin (fsk) and 50 uM genistein (gen) for 15 minutes prior to addition of PBS + NaI (replacement of NaCl with 137 mM NaI). Fluorescence was monitored every second for a total of 30 seconds (3 seconds prior to addition of NaI and 27 seconds after addition of NaI). Data were normalized to the initial fluorescence to account for variations in the overall starting fluorescence. To ensure that the observed H148Q/I152L-YFP fluorescence decrease was the result of DeltaF508-CFTR activation and not the action of additional halide channels, the CFTR specific inhibitor (CFInh-172) was used. Each experimental condition was tested in duplicate.

The data were analyzed by subtracting the basal fluorescence value from an empty well from each time point. RFU were calculated by setting the fluorescence at time zero to 1 and expressing the fluorescence at each subsequent time point as a fraction of 1.

RFU = F_T=X / F_T=0

Where:

F_T=X = fluorescence at time X
F_T=0 = fluorescence at time 0

PubChem Activity Outcome and Score:

Compounds that resulted in a 15% quenching in fluorescence, or an RFU of 0.85, at any dose or time point, were considered active.

The PubChem Activity Score is assigned a value of 100 for active compounds, and 0 for inactive compounds.

The PubChem Activity Score range for active compounds is 100-100. There are no inactive compounds.

List of reagents:

Human bronchial epithelial cells (CFBE41o-) stably expressing DeltaF508-CFTR as well as H148Q/I152L-YFP (CFBE41o- -YFP) (provided by Assay Provider)
alpha-MEM (Invitrogen, 12714010S)
Penicillin Streptomycin (Invitrogen, 15640)
FBS (Invitrogen, 10082147)
L-glutamine (Invitrogen, 21051-024)
Puromycin (Invitrogen, A1113802)
G418 (Sigma A1720)
Forskolin (Invitrogen, B-7469)
Genistein (Sigma G6649)
Comment
This assay was performed by the assay provider with powder samples of purchased test compound.
Result Definitions
Show more
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1RFU at 0 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 0 sec and 1.25 uM compound concentration; replicate [1]Floatratio
2RFU at 0 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 0 sec and 1.25 uM compound concentration; replicate [2]Floatratio
3RFU at 1 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 1 sec and 1.25 uM compound concentration; replicate [1]Floatratio
4RFU at 1 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 1 sec and 1.25 uM compound concentration; replicate [2]Floatratio
5RFU at 2 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 2 sec and 1.25 uM compound concentration; replicate [1]Floatratio
6RFU at 2 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 2 sec and 1.25 uM compound concentration; replicate [2]Floatratio
7RFU at 4 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 4 sec and 1.25 uM compound concentration; replicate [1]Floatratio
8RFU at 4 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 4 sec and 1.25 uM compound concentration; replicate [2]Floatratio
9RFU at 5 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 5 sec and 1.25 uM compound concentration; replicate [1]Floatratio
10RFU at 5 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 5 sec and 1.25 uM compound concentration; replicate [2]Floatratio
11RFU at 6 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 6 sec and 1.25 uM compound concentration; replicate [1]Floatratio
12RFU at 6 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 6 sec and 1.25 uM compound concentration; replicate [2]Floatratio
13RFU at 7 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 7 sec and 1.25 uM compound concentration; replicate [1]Floatratio
14RFU at 7 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 7 sec and 1.25 uM compound concentration; replicate [2]Floatratio
15RFU at 8 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 8 sec and 1.25 uM compound concentration; replicate [1]Floatratio
16RFU at 8 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 8 sec and 1.25 uM compound concentration; replicate [2]Floatratio
17RFU at 9 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 9 sec and 1.25 uM compound concentration; replicate [1]Floatratio
18RFU at 9 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 9 sec and 1.25 uM compound concentration; replicate [2]Floatratio
19RFU at 10 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 10 sec and 1.25 uM compound concentration; replicate [1]Floatratio
20RFU at 10 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 10 sec and 1.25 uM compound concentration; replicate [2]Floatratio
21RFU at 11 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 11 sec and 1.25 uM compound concentration; replicate [1]Floatratio
22RFU at 11 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 11 sec and 1.25 uM compound concentration; replicate [2]Floatratio
23RFU at 12 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 12 sec and 1.25 uM compound concentration; replicate [1]Floatratio
24RFU at 12 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 12 sec and 1.25 uM compound concentration; replicate [2]Floatratio
25RFU at 13 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 13 sec and 1.25 uM compound concentration; replicate [1]Floatratio
26RFU at 13 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 13 sec and 1.25 uM compound concentration; replicate [2]Floatratio
27RFU at 14 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 14 sec and 1.25 uM compound concentration; replicate [1]Floatratio
28RFU at 14 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 14 sec and 1.25 uM compound concentration; replicate [2]Floatratio
29RFU at 15 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 15 sec and 1.25 uM compound concentration; replicate [1]Floatratio
30RFU at 15 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 15 sec and 1.25 uM compound concentration; replicate [2]Floatratio
31RFU at 16 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 16 sec and 1.25 uM compound concentration; replicate [1]Floatratio
32RFU at 16 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 16 sec and 1.25 uM compound concentration; replicate [2]Floatratio
33RFU at 17 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 17 sec and 1.25 uM compound concentration; replicate [1]Floatratio
34RFU at 17 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 17 sec and 1.25 uM compound concentration; replicate [2]Floatratio
35RFU at 18 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 18 sec and 1.25 uM compound concentration; replicate [1]Floatratio
36RFU at 18 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 18 sec and 1.25 uM compound concentration; replicate [2]Floatratio
37RFU at 19 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 19 sec and 1.25 uM compound concentration; replicate [1]Floatratio
38RFU at 19 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 19 sec and 1.25 uM compound concentration; replicate [2]Floatratio
39RFU at 20 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 20 sec and 1.25 uM compound concentration; replicate [1]Floatratio
40RFU at 20 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 20 sec and 1.25 uM compound concentration; replicate [2]Floatratio
41RFU at 21 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 21 sec and 1.25 uM compound concentration; replicate [1]Floatratio
42RFU at 21 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 21 sec and 1.25 uM compound concentration; replicate [2]Floatratio
43RFU at 22 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 22 sec and 1.25 uM compound concentration; replicate [1]Floatratio
44RFU at 22 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 22 sec and 1.25 uM compound concentration; replicate [2]Floatratio
45RFU at 23 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 23 sec and 1.25 uM compound concentration; replicate [1]Floatratio
46RFU at 23 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 23 sec and 1.25 uM compound concentration; replicate [2]Floatratio
47RFU at 24 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 24 sec and 1.25 uM compound concentration; replicate [1]Floatratio
48RFU at 24 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 24 sec and 1.25 uM compound concentration; replicate [2]Floatratio
49RFU at 25 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 25 sec and 1.25 uM compound concentration; replicate [1]Floatratio
50RFU at 25 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 25 sec and 1.25 uM compound concentration; replicate [2]Floatratio
51RFU at 26 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 26 sec and 1.25 uM compound concentration; replicate [1]Floatratio
52RFU at 26 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 26 sec and 1.25 uM compound concentration; replicate [2]Floatratio
53RFU at 27 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 27 sec and 1.25 uM compound concentration; replicate [1]Floatratio
54RFU at 27 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 27 sec and 1.25 uM compound concentration; replicate [2]Floatratio
55RFU at 28 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 28 sec and 1.25 uM compound concentration; replicate [1]Floatratio
56RFU at 28 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 28 sec and 1.25 uM compound concentration; replicate [2]Floatratio
57RFU at 29 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 29 sec and 1.25 uM compound concentration; replicate [1]Floatratio
58RFU at 29 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 29 sec and 1.25 uM compound concentration; replicate [2]Floatratio
59RFU at 30 sec and 1.25 uM [1] (1.25μM**)Value of RFU at 30 sec and 1.25 uM compound concentration; replicate [1]Floatratio
60RFU at 30 sec and 1.25 uM [2] (1.25μM**)Value of RFU at 30 sec and 1.25 uM compound concentration; replicate [2]Floatratio
61RFU at 0 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 0 sec and 2.5 uM compound concentration; replicate [1]Floatratio
62RFU at 0 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 0 sec and 2.5 uM compound concentration; replicate [2]Floatratio
63RFU at 1 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 1 sec and 2.5 uM compound concentration; replicate [1]Floatratio
64RFU at 1 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 1 sec and 2.5 uM compound concentration; replicate [2]Floatratio
65RFU at 2 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 2 sec and 2.5 uM compound concentration; replicate [1]Floatratio
66RFU at 2 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 2 sec and 2.5 uM compound concentration; replicate [2]Floatratio
67RFU at 4 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 4 sec and 2.5 uM compound concentration; replicate [1]Floatratio
68RFU at 4 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 4 sec and 2.5 uM compound concentration; replicate [2]Floatratio
69RFU at 5 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 5 sec and 2.5 uM compound concentration; replicate [1]Floatratio
70RFU at 5 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 5 sec and 2.5 uM compound concentration; replicate [2]Floatratio
71RFU at 6 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 6 sec and 2.5 uM compound concentration; replicate [1]Floatratio
72RFU at 6 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 6 sec and 2.5 uM compound concentration; replicate [2]Floatratio
73RFU at 7 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 7 sec and 2.5 uM compound concentration; replicate [1]Floatratio
74RFU at 7 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 7 sec and 2.5 uM compound concentration; replicate [2]Floatratio
75RFU at 8 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 8 sec and 2.5 uM compound concentration; replicate [1]Floatratio
76RFU at 8 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 8 sec and 2.5 uM compound concentration; replicate [2]Floatratio
77RFU at 9 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 9 sec and 2.5 uM compound concentration; replicate [1]Floatratio
78RFU at 9 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 9 sec and 2.5 uM compound concentration; replicate [2]Floatratio
79RFU at 10 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 10 sec and 2.5 uM compound concentration; replicate [1]Floatratio
80RFU at 10 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 10 sec and 2.5 uM compound concentration; replicate [2]Floatratio
81RFU at 11 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 11 sec and 2.5 uM compound concentration; replicate [1]Floatratio
82RFU at 11 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 11 sec and 2.5 uM compound concentration; replicate [2]Floatratio
83RFU at 12 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 12 sec and 2.5 uM compound concentration; replicate [1]Floatratio
84RFU at 12 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 12 sec and 2.5 uM compound concentration; replicate [2]Floatratio
85RFU at 13 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 13 sec and 2.5 uM compound concentration; replicate [1]Floatratio
86RFU at 13 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 13 sec and 2.5 uM compound concentration; replicate [2]Floatratio
87RFU at 14 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 14 sec and 2.5 uM compound concentration; replicate [1]Floatratio
88RFU at 14 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 14 sec and 2.5 uM compound concentration; replicate [2]Floatratio
89RFU at 15 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 15 sec and 2.5 uM compound concentration; replicate [1]Floatratio
90RFU at 15 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 15 sec and 2.5 uM compound concentration; replicate [2]Floatratio
91RFU at 16 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 16 sec and 2.5 uM compound concentration; replicate [1]Floatratio
92RFU at 16 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 16 sec and 2.5 uM compound concentration; replicate [2]Floatratio
93RFU at 17 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 17 sec and 2.5 uM compound concentration; replicate [1]Floatratio
94RFU at 17 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 17 sec and 2.5 uM compound concentration; replicate [2]Floatratio
95RFU at 18 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 18 sec and 2.5 uM compound concentration; replicate [1]Floatratio
96RFU at 18 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 18 sec and 2.5 uM compound concentration; replicate [2]Floatratio
97RFU at 19 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 19 sec and 2.5 uM compound concentration; replicate [1]Floatratio
98RFU at 19 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 19 sec and 2.5 uM compound concentration; replicate [2]Floatratio
99RFU at 20 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 20 sec and 2.5 uM compound concentration; replicate [1]Floatratio
100RFU at 20 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 20 sec and 2.5 uM compound concentration; replicate [2]Floatratio
101RFU at 21 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 21 sec and 2.5 uM compound concentration; replicate [1]Floatratio
102RFU at 21 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 21 sec and 2.5 uM compound concentration; replicate [2]Floatratio
103RFU at 22 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 22 sec and 2.5 uM compound concentration; replicate [1]Floatratio
104RFU at 22 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 22 sec and 2.5 uM compound concentration; replicate [2]Floatratio
105RFU at 23 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 23 sec and 2.5 uM compound concentration; replicate [1]Floatratio
106RFU at 23 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 23 sec and 2.5 uM compound concentration; replicate [2]Floatratio
107RFU at 24 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 24 sec and 2.5 uM compound concentration; replicate [1]Floatratio
108RFU at 24 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 24 sec and 2.5 uM compound concentration; replicate [2]Floatratio
109RFU at 25 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 25 sec and 2.5 uM compound concentration; replicate [1]Floatratio
110RFU at 25 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 25 sec and 2.5 uM compound concentration; replicate [2]Floatratio
111RFU at 26 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 26 sec and 2.5 uM compound concentration; replicate [1]Floatratio
112RFU at 26 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 26 sec and 2.5 uM compound concentration; replicate [2]Floatratio
113RFU at 27 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 27 sec and 2.5 uM compound concentration; replicate [1]Floatratio
114RFU at 27 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 27 sec and 2.5 uM compound concentration; replicate [2]Floatratio
115RFU at 28 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 28 sec and 2.5 uM compound concentration; replicate [1]Floatratio
116RFU at 28 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 28 sec and 2.5 uM compound concentration; replicate [2]Floatratio
117RFU at 29 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 29 sec and 2.5 uM compound concentration; replicate [1]Floatratio
118RFU at 29 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 29 sec and 2.5 uM compound concentration; replicate [2]Floatratio
119RFU at 30 sec and 2.5 uM [1] (2.5μM**)Value of RFU at 30 sec and 2.5 uM compound concentration; replicate [1]Floatratio
120RFU at 30 sec and 2.5 uM [2] (2.5μM**)Value of RFU at 30 sec and 2.5 uM compound concentration; replicate [2]Floatratio
121RFU at 0 sec and 5 uM [1] (5μM**)Value of RFU at 0 sec and 5 uM compound concentration; replicate [1]Floatratio
122RFU at 0 sec and 5 uM [2] (5μM**)Value of RFU at 0 sec and 5 uM compound concentration; replicate [2]Floatratio
123RFU at 1 sec and 5 uM [1] (5μM**)Value of RFU at 1 sec and 5 uM compound concentration; replicate [1]Floatratio
124RFU at 1 sec and 5 uM [2] (5μM**)Value of RFU at 1 sec and 5 uM compound concentration; replicate [2]Floatratio
125RFU at 2 sec and 5 uM [1] (5μM**)Value of RFU at 2 sec and 5 uM compound concentration; replicate [1]Floatratio
126RFU at 2 sec and 5 uM [2] (5μM**)Value of RFU at 2 sec and 5 uM compound concentration; replicate [2]Floatratio
127RFU at 4 sec and 5 uM [1] (5μM**)Value of RFU at 4 sec and 5 uM compound concentration; replicate [1]Floatratio
128RFU at 4 sec and 5 uM [2] (5μM**)Value of RFU at 4 sec and 5 uM compound concentration; replicate [2]Floatratio
129RFU at 5 sec and 5 uM [1] (5μM**)Value of RFU at 5 sec and 5 uM compound concentration; replicate [1]Floatratio
130RFU at 5 sec and 5 uM [2] (5μM**)Value of RFU at 5 sec and 5 uM compound concentration; replicate [2]Floatratio
131RFU at 6 sec and 5 uM [1] (5μM**)Value of RFU at 6 sec and 5 uM compound concentration; replicate [1]Floatratio
132RFU at 6 sec and 5 uM [2] (5μM**)Value of RFU at 6 sec and 5 uM compound concentration; replicate [2]Floatratio
133RFU at 7 sec and 5 uM [1] (5μM**)Value of RFU at 7 sec and 5 uM compound concentration; replicate [1]Floatratio
134RFU at 7 sec and 5 uM [2] (5μM**)Value of RFU at 7 sec and 5 uM compound concentration; replicate [2]Floatratio
135RFU at 8 sec and 5 uM [1] (5μM**)Value of RFU at 8 sec and 5 uM compound concentration; replicate [1]Floatratio
136RFU at 8 sec and 5 uM [2] (5μM**)Value of RFU at 8 sec and 5 uM compound concentration; replicate [2]Floatratio
137RFU at 9 sec and 5 uM [1] (5μM**)Value of RFU at 9 sec and 5 uM compound concentration; replicate [1]Floatratio
138RFU at 9 sec and 5 uM [2] (5μM**)Value of RFU at 9 sec and 5 uM compound concentration; replicate [2]Floatratio
139RFU at 10 sec and 5 uM [1] (5μM**)Value of RFU at 10 sec and 5 uM compound concentration; replicate [1]Floatratio
140RFU at 10 sec and 5 uM [2] (5μM**)Value of RFU at 10 sec and 5 uM compound concentration; replicate [2]Floatratio
141RFU at 11 sec and 5 uM [1] (5μM**)Value of RFU at 11 sec and 5 uM compound concentration; replicate [1]Floatratio
142RFU at 11 sec and 5 uM [2] (5μM**)Value of RFU at 11 sec and 5 uM compound concentration; replicate [2]Floatratio
143RFU at 12 sec and 5 uM [1] (5μM**)Value of RFU at 12 sec and 5 uM compound concentration; replicate [1]Floatratio
144RFU at 12 sec and 5 uM [2] (5μM**)Value of RFU at 12 sec and 5 uM compound concentration; replicate [2]Floatratio
145RFU at 13 sec and 5 uM [1] (5μM**)Value of RFU at 13 sec and 5 uM compound concentration; replicate [1]Floatratio
146RFU at 13 sec and 5 uM [2] (5μM**)Value of RFU at 13 sec and 5 uM compound concentration; replicate [2]Floatratio
147RFU at 14 sec and 5 uM [1] (5μM**)Value of RFU at 14 sec and 5 uM compound concentration; replicate [1]Floatratio
148RFU at 14 sec and 5 uM [2] (5μM**)Value of RFU at 14 sec and 5 uM compound concentration; replicate [2]Floatratio
149RFU at 15 sec and 5 uM [1] (5μM**)Value of RFU at 15 sec and 5 uM compound concentration; replicate [1]Floatratio
150RFU at 15 sec and 5 uM [2] (5μM**)Value of RFU at 15 sec and 5 uM compound concentration; replicate [2]Floatratio
151RFU at 16 sec and 5 uM [1] (5μM**)Value of RFU at 16 sec and 5 uM compound concentration; replicate [1]Floatratio
152RFU at 16 sec and 5 uM [2] (5μM**)Value of RFU at 16 sec and 5 uM compound concentration; replicate [2]Floatratio
153RFU at 17 sec and 5 uM [1] (5μM**)Value of RFU at 17 sec and 5 uM compound concentration; replicate [1]Floatratio
154RFU at 17 sec and 5 uM [2] (5μM**)Value of RFU at 17 sec and 5 uM compound concentration; replicate [2]Floatratio
155RFU at 18 sec and 5 uM [1] (5μM**)Value of RFU at 18 sec and 5 uM compound concentration; replicate [1]Floatratio
156RFU at 18 sec and 5 uM [2] (5μM**)Value of RFU at 18 sec and 5 uM compound concentration; replicate [2]Floatratio
157RFU at 19 sec and 5 uM [1] (5μM**)Value of RFU at 19 sec and 5 uM compound concentration; replicate [1]Floatratio
158RFU at 19 sec and 5 uM [2] (5μM**)Value of RFU at 19 sec and 5 uM compound concentration; replicate [2]Floatratio
159RFU at 20 sec and 5 uM [1] (5μM**)Value of RFU at 20 sec and 5 uM compound concentration; replicate [1]Floatratio
160RFU at 20 sec and 5 uM [2] (5μM**)Value of RFU at 20 sec and 5 uM compound concentration; replicate [2]Floatratio
161RFU at 21 sec and 5 uM [1] (5μM**)Value of RFU at 21 sec and 5 uM compound concentration; replicate [1]Floatratio
162RFU at 21 sec and 5 uM [2] (5μM**)Value of RFU at 21 sec and 5 uM compound concentration; replicate [2]Floatratio
163RFU at 22 sec and 5 uM [1] (5μM**)Value of RFU at 22 sec and 5 uM compound concentration; replicate [1]Floatratio
164RFU at 22 sec and 5 uM [2] (5μM**)Value of RFU at 22 sec and 5 uM compound concentration; replicate [2]Floatratio
165RFU at 23 sec and 5 uM [1] (5μM**)Value of RFU at 23 sec and 5 uM compound concentration; replicate [1]Floatratio
166RFU at 23 sec and 5 uM [2] (5μM**)Value of RFU at 23 sec and 5 uM compound concentration; replicate [2]Floatratio
167RFU at 24 sec and 5 uM [1] (5μM**)Value of RFU at 24 sec and 5 uM compound concentration; replicate [1]Floatratio
168RFU at 24 sec and 5 uM [2] (5μM**)Value of RFU at 24 sec and 5 uM compound concentration; replicate [2]Floatratio
169RFU at 25 sec and 5 uM [1] (5μM**)Value of RFU at 25 sec and 5 uM compound concentration; replicate [1]Floatratio
170RFU at 25 sec and 5 uM [2] (5μM**)Value of RFU at 25 sec and 5 uM compound concentration; replicate [2]Floatratio
171RFU at 26 sec and 5 uM [1] (5μM**)Value of RFU at 26 sec and 5 uM compound concentration; replicate [1]Floatratio
172RFU at 26 sec and 5 uM [2] (5μM**)Value of RFU at 26 sec and 5 uM compound concentration; replicate [2]Floatratio
173RFU at 27 sec and 5 uM [1] (5μM**)Value of RFU at 27 sec and 5 uM compound concentration; replicate [1]Floatratio
174RFU at 27 sec and 5 uM [2] (5μM**)Value of RFU at 27 sec and 5 uM compound concentration; replicate [2]Floatratio
175RFU at 28 sec and 5 uM [1] (5μM**)Value of RFU at 28 sec and 5 uM compound concentration; replicate [1]Floatratio
176RFU at 28 sec and 5 uM [2] (5μM**)Value of RFU at 28 sec and 5 uM compound concentration; replicate [2]Floatratio
177RFU at 29 sec and 5 uM [1] (5μM**)Value of RFU at 29 sec and 5 uM compound concentration; replicate [1]Floatratio
178RFU at 29 sec and 5 uM [2] (5μM**)Value of RFU at 29 sec and 5 uM compound concentration; replicate [2]Floatratio
179RFU at 30 sec and 5 uM [1] (5μM**)Value of RFU at 30 sec and 5 uM compound concentration; replicate [1]Floatratio
180RFU at 30 sec and 5 uM [2] (5μM**)Value of RFU at 30 sec and 5 uM compound concentration; replicate [2]Floatratio
181RFU at 0 sec and 10 uM [1] (10μM**)Value of RFU at 0 sec and 10 uM compound concentration; replicate [1]Floatratio
182RFU at 0 sec and 10 uM [2] (10μM**)Value of RFU at 0 sec and 10 uM compound concentration; replicate [2]Floatratio
183RFU at 1 sec and 10 uM [1] (10μM**)Value of RFU at 1 sec and 10 uM compound concentration; replicate [1]Floatratio
184RFU at 1 sec and 10 uM [2] (10μM**)Value of RFU at 1 sec and 10 uM compound concentration; replicate [2]Floatratio
185RFU at 2 sec and 10 uM [1] (10μM**)Value of RFU at 2 sec and 10 uM compound concentration; replicate [1]Floatratio
186RFU at 2 sec and 10 uM [2] (10μM**)Value of RFU at 2 sec and 10 uM compound concentration; replicate [2]Floatratio
187RFU at 4 sec and 10 uM [1] (10μM**)Value of RFU at 4 sec and 10 uM compound concentration; replicate [1]Floatratio
188RFU at 4 sec and 10 uM [2] (10μM**)Value of RFU at 4 sec and 10 uM compound concentration; replicate [2]Floatratio
189RFU at 5 sec and 10 uM [1] (10μM**)Value of RFU at 5 sec and 10 uM compound concentration; replicate [1]Floatratio
190RFU at 5 sec and 10 uM [2] (10μM**)Value of RFU at 5 sec and 10 uM compound concentration; replicate [2]Floatratio
191RFU at 6 sec and 10 uM [1] (10μM**)Value of RFU at 6 sec and 10 uM compound concentration; replicate [1]Floatratio
192RFU at 6 sec and 10 uM [2] (10μM**)Value of RFU at 6 sec and 10 uM compound concentration; replicate [2]Floatratio
193RFU at 7 sec and 10 uM [1] (10μM**)Value of RFU at 7 sec and 10 uM compound concentration; replicate [1]Floatratio
194RFU at 7 sec and 10 uM [2] (10μM**)Value of RFU at 7 sec and 10 uM compound concentration; replicate [2]Floatratio
195RFU at 8 sec and 10 uM [1] (10μM**)Value of RFU at 8 sec and 10 uM compound concentration; replicate [1]Floatratio
196RFU at 8 sec and 10 uM [2] (10μM**)Value of RFU at 8 sec and 10 uM compound concentration; replicate [2]Floatratio
197RFU at 9 sec and 10 uM [1] (10μM**)Value of RFU at 9 sec and 10 uM compound concentration; replicate [1]Floatratio
198RFU at 9 sec and 10 uM [2] (10μM**)Value of RFU at 9 sec and 10 uM compound concentration; replicate [2]Floatratio
199RFU at 10 sec and 10 uM [1] (10μM**)Value of RFU at 10 sec and 10 uM compound concentration; replicate [1]Floatratio
200RFU at 10 sec and 10 uM [2] (10μM**)Value of RFU at 10 sec and 10 uM compound concentration; replicate [2]Floatratio
201RFU at 11 sec and 10 uM [1] (10μM**)Value of RFU at 11 sec and 10 uM compound concentration; replicate [1]Floatratio
202RFU at 11 sec and 10 uM [2] (10μM**)Value of RFU at 11 sec and 10 uM compound concentration; replicate [2]Floatratio
203RFU at 12 sec and 10 uM [1] (10μM**)Value of RFU at 12 sec and 10 uM compound concentration; replicate [1]Floatratio
204RFU at 12 sec and 10 uM [2] (10μM**)Value of RFU at 12 sec and 10 uM compound concentration; replicate [2]Floatratio
205RFU at 13 sec and 10 uM [1] (10μM**)Value of RFU at 13 sec and 10 uM compound concentration; replicate [1]Floatratio
206RFU at 13 sec and 10 uM [2] (10μM**)Value of RFU at 13 sec and 10 uM compound concentration; replicate [2]Floatratio
207RFU at 14 sec and 10 uM [1] (10μM**)Value of RFU at 14 sec and 10 uM compound concentration; replicate [1]Floatratio
208RFU at 14 sec and 10 uM [2] (10μM**)Value of RFU at 14 sec and 10 uM compound concentration; replicate [2]Floatratio
209RFU at 15 sec and 10 uM [1] (10μM**)Value of RFU at 15 sec and 10 uM compound concentration; replicate [1]Floatratio
210RFU at 15 sec and 10 uM [2] (10μM**)Value of RFU at 15 sec and 10 uM compound concentration; replicate [2]Floatratio
211RFU at 16 sec and 10 uM [1] (10μM**)Value of RFU at 16 sec and 10 uM compound concentration; replicate [1]Floatratio
212RFU at 16 sec and 10 uM [2] (10μM**)Value of RFU at 16 sec and 10 uM compound concentration; replicate [2]Floatratio
213RFU at 17 sec and 10 uM [1] (10μM**)Value of RFU at 17 sec and 10 uM compound concentration; replicate [1]Floatratio
214RFU at 17 sec and 10 uM [2] (10μM**)Value of RFU at 17 sec and 10 uM compound concentration; replicate [2]Floatratio
215RFU at 18 sec and 10 uM [1] (10μM**)Value of RFU at 18 sec and 10 uM compound concentration; replicate [1]Floatratio
216RFU at 18 sec and 10 uM [2] (10μM**)Value of RFU at 18 sec and 10 uM compound concentration; replicate [2]Floatratio
217RFU at 19 sec and 10 uM [1] (10μM**)Value of RFU at 19 sec and 10 uM compound concentration; replicate [1]Floatratio
218RFU at 19 sec and 10 uM [2] (10μM**)Value of RFU at 19 sec and 10 uM compound concentration; replicate [2]Floatratio
219RFU at 20 sec and 10 uM [1] (10μM**)Value of RFU at 20 sec and 10 uM compound concentration; replicate [1]Floatratio
220RFU at 20 sec and 10 uM [2] (10μM**)Value of RFU at 20 sec and 10 uM compound concentration; replicate [2]Floatratio
221RFU at 21 sec and 10 uM [1] (10μM**)Value of RFU at 21 sec and 10 uM compound concentration; replicate [1]Floatratio
222RFU at 21 sec and 10 uM [2] (10μM**)Value of RFU at 21 sec and 10 uM compound concentration; replicate [2]Floatratio
223RFU at 22 sec and 10 uM [1] (10μM**)Value of RFU at 22 sec and 10 uM compound concentration; replicate [1]Floatratio
224RFU at 22 sec and 10 uM [2] (10μM**)Value of RFU at 22 sec and 10 uM compound concentration; replicate [2]Floatratio
225RFU at 23 sec and 10 uM [1] (10μM**)Value of RFU at 23 sec and 10 uM compound concentration; replicate [1]Floatratio
226RFU at 23 sec and 10 uM [2] (10μM**)Value of RFU at 23 sec and 10 uM compound concentration; replicate [2]Floatratio
227RFU at 24 sec and 10 uM [1] (10μM**)Value of RFU at 24 sec and 10 uM compound concentration; replicate [1]Floatratio
228RFU at 24 sec and 10 uM [2] (10μM**)Value of RFU at 24 sec and 10 uM compound concentration; replicate [2]Floatratio
229RFU at 25 sec and 10 uM [1] (10μM**)Value of RFU at 25 sec and 10 uM compound concentration; replicate [1]Floatratio
230RFU at 25 sec and 10 uM [2] (10μM**)Value of RFU at 25 sec and 10 uM compound concentration; replicate [2]Floatratio
231RFU at 26 sec and 10 uM [1] (10μM**)Value of RFU at 26 sec and 10 uM compound concentration; replicate [1]Floatratio
232RFU at 26 sec and 10 uM [2] (10μM**)Value of RFU at 26 sec and 10 uM compound concentration; replicate [2]Floatratio
233RFU at 27 sec and 10 uM [1] (10μM**)Value of RFU at 27 sec and 10 uM compound concentration; replicate [1]Floatratio
234RFU at 27 sec and 10 uM [2] (10μM**)Value of RFU at 27 sec and 10 uM compound concentration; replicate [2]Floatratio
235RFU at 28 sec and 10 uM [1] (10μM**)Value of RFU at 28 sec and 10 uM compound concentration; replicate [1]Floatratio
236RFU at 28 sec and 10 uM [2] (10μM**)Value of RFU at 28 sec and 10 uM compound concentration; replicate [2]Floatratio
237RFU at 29 sec and 10 uM [1] (10μM**)Value of RFU at 29 sec and 10 uM compound concentration; replicate [1]Floatratio
238RFU at 29 sec and 10 uM [2] (10μM**)Value of RFU at 29 sec and 10 uM compound concentration; replicate [2]Floatratio
239RFU at 30 sec and 10 uM [1] (10μM**)Value of RFU at 30 sec and 10 uM compound concentration; replicate [1]Floatratio
240RFU at 30 sec and 10 uM [2] (10μM**)Value of RFU at 30 sec and 10 uM compound concentration; replicate [2]Floatratio
241RFU at 0 sec and 20 uM [1] (20μM**)Value of RFU at 0 sec and 20 uM compound concentration; replicate [1]Floatratio
242RFU at 0 sec and 20 uM [2] (20μM**)Value of RFU at 0 sec and 20 uM compound concentration; replicate [2]Floatratio
243RFU at 1 sec and 20 uM [1] (20μM**)Value of RFU at 1 sec and 20 uM compound concentration; replicate [1]Floatratio
244RFU at 1 sec and 20 uM [2] (20μM**)Value of RFU at 1 sec and 20 uM compound concentration; replicate [2]Floatratio
245RFU at 2 sec and 20 uM [1] (20μM**)Value of RFU at 2 sec and 20 uM compound concentration; replicate [1]Floatratio
246RFU at 2 sec and 20 uM [2] (20μM**)Value of RFU at 2 sec and 20 uM compound concentration; replicate [2]Floatratio
247RFU at 4 sec and 20 uM [1] (20μM**)Value of RFU at 4 sec and 20 uM compound concentration; replicate [1]Floatratio
248RFU at 4 sec and 20 uM [2] (20μM**)Value of RFU at 4 sec and 20 uM compound concentration; replicate [2]Floatratio
249RFU at 5 sec and 20 uM [1] (20μM**)Value of RFU at 5 sec and 20 uM compound concentration; replicate [1]Floatratio
250RFU at 5 sec and 20 uM [2] (20μM**)Value of RFU at 5 sec and 20 uM compound concentration; replicate [2]Floatratio
251RFU at 6 sec and 20 uM [1] (20μM**)Value of RFU at 6 sec and 20 uM compound concentration; replicate [1]Floatratio
252RFU at 6 sec and 20 uM [2] (20μM**)Value of RFU at 6 sec and 20 uM compound concentration; replicate [2]Floatratio
253RFU at 7 sec and 20 uM [1] (20μM**)Value of RFU at 7 sec and 20 uM compound concentration; replicate [1]Floatratio
254RFU at 7 sec and 20 uM [2] (20μM**)Value of RFU at 7 sec and 20 uM compound concentration; replicate [2]Floatratio
255RFU at 8 sec and 20 uM [1] (20μM**)Value of RFU at 8 sec and 20 uM compound concentration; replicate [1]Floatratio
256RFU at 8 sec and 20 uM [2] (20μM**)Value of RFU at 8 sec and 20 uM compound concentration; replicate [2]Floatratio
257RFU at 9 sec and 20 uM [1] (20μM**)Value of RFU at 9 sec and 20 uM compound concentration; replicate [1]Floatratio
258RFU at 9 sec and 20 uM [2] (20μM**)Value of RFU at 9 sec and 20 uM compound concentration; replicate [2]Floatratio
259RFU at 10 sec and 20 uM [1] (20μM**)Value of RFU at 10 sec and 20 uM compound concentration; replicate [1]Floatratio
260RFU at 10 sec and 20 uM [2] (20μM**)Value of RFU at 10 sec and 20 uM compound concentration; replicate [2]Floatratio
261RFU at 11 sec and 20 uM [1] (20μM**)Value of RFU at 11 sec and 20 uM compound concentration; replicate [1]Floatratio
262RFU at 11 sec and 20 uM [2] (20μM**)Value of RFU at 11 sec and 20 uM compound concentration; replicate [2]Floatratio
263RFU at 12 sec and 20 uM [1] (20μM**)Value of RFU at 12 sec and 20 uM compound concentration; replicate [1]Floatratio
264RFU at 12 sec and 20 uM [2] (20μM**)Value of RFU at 12 sec and 20 uM compound concentration; replicate [2]Floatratio
265RFU at 13 sec and 20 uM [1] (20μM**)Value of RFU at 13 sec and 20 uM compound concentration; replicate [1]Floatratio
266RFU at 13 sec and 20 uM [2] (20μM**)Value of RFU at 13 sec and 20 uM compound concentration; replicate [2]Floatratio
267RFU at 14 sec and 20 uM [1] (20μM**)Value of RFU at 14 sec and 20 uM compound concentration; replicate [1]Floatratio
268RFU at 14 sec and 20 uM [2] (20μM**)Value of RFU at 14 sec and 20 uM compound concentration; replicate [2]Floatratio
269RFU at 15 sec and 20 uM [1] (20μM**)Value of RFU at 15 sec and 20 uM compound concentration; replicate [1]Floatratio
270RFU at 15 sec and 20 uM [2] (20μM**)Value of RFU at 15 sec and 20 uM compound concentration; replicate [2]Floatratio
271RFU at 16 sec and 20 uM [1] (20μM**)Value of RFU at 16 sec and 20 uM compound concentration; replicate [1]Floatratio
272RFU at 16 sec and 20 uM [2] (20μM**)Value of RFU at 16 sec and 20 uM compound concentration; replicate [2]Floatratio
273RFU at 17 sec and 20 uM [1] (20μM**)Value of RFU at 17 sec and 20 uM compound concentration; replicate [1]Floatratio
274RFU at 17 sec and 20 uM [2] (20μM**)Value of RFU at 17 sec and 20 uM compound concentration; replicate [2]Floatratio
275RFU at 18 sec and 20 uM [1] (20μM**)Value of RFU at 18 sec and 20 uM compound concentration; replicate [1]Floatratio
276RFU at 18 sec and 20 uM [2] (20μM**)Value of RFU at 18 sec and 20 uM compound concentration; replicate [2]Floatratio
277RFU at 19 sec and 20 uM [1] (20μM**)Value of RFU at 19 sec and 20 uM compound concentration; replicate [1]Floatratio
278RFU at 19 sec and 20 uM [2] (20μM**)Value of RFU at 19 sec and 20 uM compound concentration; replicate [2]Floatratio
279RFU at 20 sec and 20 uM [1] (20μM**)Value of RFU at 20 sec and 20 uM compound concentration; replicate [1]Floatratio
280RFU at 20 sec and 20 uM [2] (20μM**)Value of RFU at 20 sec and 20 uM compound concentration; replicate [2]Floatratio
281RFU at 21 sec and 20 uM [1] (20μM**)Value of RFU at 21 sec and 20 uM compound concentration; replicate [1]Floatratio
282RFU at 21 sec and 20 uM [2] (20μM**)Value of RFU at 21 sec and 20 uM compound concentration; replicate [2]Floatratio
283RFU at 22 sec and 20 uM [1] (20μM**)Value of RFU at 22 sec and 20 uM compound concentration; replicate [1]Floatratio
284RFU at 22 sec and 20 uM [2] (20μM**)Value of RFU at 22 sec and 20 uM compound concentration; replicate [2]Floatratio
285RFU at 23 sec and 20 uM [1] (20μM**)Value of RFU at 23 sec and 20 uM compound concentration; replicate [1]Floatratio
286RFU at 23 sec and 20 uM [2] (20μM**)Value of RFU at 23 sec and 20 uM compound concentration; replicate [2]Floatratio
287RFU at 24 sec and 20 uM [1] (20μM**)Value of RFU at 24 sec and 20 uM compound concentration; replicate [1]Floatratio
288RFU at 24 sec and 20 uM [2] (20μM**)Value of RFU at 24 sec and 20 uM compound concentration; replicate [2]Floatratio
289RFU at 25 sec and 20 uM [1] (20μM**)Value of RFU at 25 sec and 20 uM compound concentration; replicate [1]Floatratio
290RFU at 25 sec and 20 uM [2] (20μM**)Value of RFU at 25 sec and 20 uM compound concentration; replicate [2]Floatratio
291RFU at 26 sec and 20 uM [1] (20μM**)Value of RFU at 26 sec and 20 uM compound concentration; replicate [1]Floatratio
292RFU at 26 sec and 20 uM [2] (20μM**)Value of RFU at 26 sec and 20 uM compound concentration; replicate [2]Floatratio
293RFU at 27 sec and 20 uM [1] (20μM**)Value of RFU at 27 sec and 20 uM compound concentration; replicate [1]Floatratio
294RFU at 27 sec and 20 uM [2] (20μM**)Value of RFU at 27 sec and 20 uM compound concentration; replicate [2]Floatratio
295RFU at 28 sec and 20 uM [1] (20μM**)Value of RFU at 28 sec and 20 uM compound concentration; replicate [1]Floatratio
296RFU at 28 sec and 20 uM [2] (20μM**)Value of RFU at 28 sec and 20 uM compound concentration; replicate [2]Floatratio
297RFU at 29 sec and 20 uM [1] (20μM**)Value of RFU at 29 sec and 20 uM compound concentration; replicate [1]Floatratio
298RFU at 29 sec and 20 uM [2] (20μM**)Value of RFU at 29 sec and 20 uM compound concentration; replicate [2]Floatratio
299RFU at 30 sec and 20 uM [1] (20μM**)Value of RFU at 30 sec and 20 uM compound concentration; replicate [1]Floatratio
300RFU at 30 sec and 20 uM [2] (20μM**)Value of RFU at 30 sec and 20 uM compound concentration; replicate [2]Floatratio
301RFU at 0 sec and 40 uM [1] (40μM**)Value of RFU at 0 sec and 40 uM compound concentration; replicate [1]Floatratio
302RFU at 0 sec and 40 uM [2] (40μM**)Value of RFU at 0 sec and 40 uM compound concentration; replicate [2]Floatratio
303RFU at 1 sec and 40 uM [1] (40μM**)Value of RFU at 1 sec and 40 uM compound concentration; replicate [1]Floatratio
304RFU at 1 sec and 40 uM [2] (40μM**)Value of RFU at 1 sec and 40 uM compound concentration; replicate [2]Floatratio
305RFU at 2 sec and 40 uM [1] (40μM**)Value of RFU at 2 sec and 40 uM compound concentration; replicate [1]Floatratio
306RFU at 2 sec and 40 uM [2] (40μM**)Value of RFU at 2 sec and 40 uM compound concentration; replicate [2]Floatratio
307RFU at 4 sec and 40 uM [1] (40μM**)Value of RFU at 4 sec and 40 uM compound concentration; replicate [1]Floatratio
308RFU at 4 sec and 40 uM [2] (40μM**)Value of RFU at 4 sec and 40 uM compound concentration; replicate [2]Floatratio
309RFU at 5 sec and 40 uM [1] (40μM**)Value of RFU at 5 sec and 40 uM compound concentration; replicate [1]Floatratio
310RFU at 5 sec and 40 uM [2] (40μM**)Value of RFU at 5 sec and 40 uM compound concentration; replicate [2]Floatratio
311RFU at 6 sec and 40 uM [1] (40μM**)Value of RFU at 6 sec and 40 uM compound concentration; replicate [1]Floatratio
312RFU at 6 sec and 40 uM [2] (40μM**)Value of RFU at 6 sec and 40 uM compound concentration; replicate [2]Floatratio
313RFU at 7 sec and 40 uM [1] (40μM**)Value of RFU at 7 sec and 40 uM compound concentration; replicate [1]Floatratio
314RFU at 7 sec and 40 uM [2] (40μM**)Value of RFU at 7 sec and 40 uM compound concentration; replicate [2]Floatratio
315RFU at 8 sec and 40 uM [1] (40μM**)Value of RFU at 8 sec and 40 uM compound concentration; replicate [1]Floatratio
316RFU at 8 sec and 40 uM [2] (40μM**)Value of RFU at 8 sec and 40 uM compound concentration; replicate [2]Floatratio
317RFU at 9 sec and 40 uM [1] (40μM**)Value of RFU at 9 sec and 40 uM compound concentration; replicate [1]Floatratio
318RFU at 9 sec and 40 uM [2] (40μM**)Value of RFU at 9 sec and 40 uM compound concentration; replicate [2]Floatratio
319RFU at 10 sec and 40 uM [1] (40μM**)Value of RFU at 10 sec and 40 uM compound concentration; replicate [1]Floatratio
320RFU at 10 sec and 40 uM [2] (40μM**)Value of RFU at 10 sec and 40 uM compound concentration; replicate [2]Floatratio
321RFU at 11 sec and 40 uM [1] (40μM**)Value of RFU at 11 sec and 40 uM compound concentration; replicate [1]Floatratio
322RFU at 11 sec and 40 uM [2] (40μM**)Value of RFU at 11 sec and 40 uM compound concentration; replicate [2]Floatratio
323RFU at 12 sec and 40 uM [1] (40μM**)Value of RFU at 12 sec and 40 uM compound concentration; replicate [1]Floatratio
324RFU at 12 sec and 40 uM [2] (40μM**)Value of RFU at 12 sec and 40 uM compound concentration; replicate [2]Floatratio
325RFU at 13 sec and 40 uM [1] (40μM**)Value of RFU at 13 sec and 40 uM compound concentration; replicate [1]Floatratio
326RFU at 13 sec and 40 uM [2] (40μM**)Value of RFU at 13 sec and 40 uM compound concentration; replicate [2]Floatratio
327RFU at 14 sec and 40 uM [1] (40μM**)Value of RFU at 14 sec and 40 uM compound concentration; replicate [1]Floatratio
328RFU at 14 sec and 40 uM [2] (40μM**)Value of RFU at 14 sec and 40 uM compound concentration; replicate [2]Floatratio
329RFU at 15 sec and 40 uM [1] (40μM**)Value of RFU at 15 sec and 40 uM compound concentration; replicate [1]Floatratio
330RFU at 15 sec and 40 uM [2] (40μM**)Value of RFU at 15 sec and 40 uM compound concentration; replicate [2]Floatratio
331RFU at 16 sec and 40 uM [1] (40μM**)Value of RFU at 16 sec and 40 uM compound concentration; replicate [1]Floatratio
332RFU at 16 sec and 40 uM [2] (40μM**)Value of RFU at 16 sec and 40 uM compound concentration; replicate [2]Floatratio
333RFU at 17 sec and 40 uM [1] (40μM**)Value of RFU at 17 sec and 40 uM compound concentration; replicate [1]Floatratio
334RFU at 17 sec and 40 uM [2] (40μM**)Value of RFU at 17 sec and 40 uM compound concentration; replicate [2]Floatratio
335RFU at 18 sec and 40 uM [1] (40μM**)Value of RFU at 18 sec and 40 uM compound concentration; replicate [1]Floatratio
336RFU at 18 sec and 40 uM [2] (40μM**)Value of RFU at 18 sec and 40 uM compound concentration; replicate [2]Floatratio
337RFU at 19 sec and 40 uM [1] (40μM**)Value of RFU at 19 sec and 40 uM compound concentration; replicate [1]Floatratio
338RFU at 19 sec and 40 uM [2] (40μM**)Value of RFU at 19 sec and 40 uM compound concentration; replicate [2]Floatratio
339RFU at 20 sec and 40 uM [1] (40μM**)Value of RFU at 20 sec and 40 uM compound concentration; replicate [1]Floatratio
340RFU at 20 sec and 40 uM [2] (40μM**)Value of RFU at 20 sec and 40 uM compound concentration; replicate [2]Floatratio
341RFU at 21 sec and 40 uM [1] (40μM**)Value of RFU at 21 sec and 40 uM compound concentration; replicate [1]Floatratio
342RFU at 21 sec and 40 uM [2] (40μM**)Value of RFU at 21 sec and 40 uM compound concentration; replicate [2]Floatratio
343RFU at 22 sec and 40 uM [1] (40μM**)Value of RFU at 22 sec and 40 uM compound concentration; replicate [1]Floatratio
344RFU at 22 sec and 40 uM [2] (40μM**)Value of RFU at 22 sec and 40 uM compound concentration; replicate [2]Floatratio
345RFU at 23 sec and 40 uM [1] (40μM**)Value of RFU at 23 sec and 40 uM compound concentration; replicate [1]Floatratio
346RFU at 23 sec and 40 uM [2] (40μM**)Value of RFU at 23 sec and 40 uM compound concentration; replicate [2]Floatratio
347RFU at 24 sec and 40 uM [1] (40μM**)Value of RFU at 24 sec and 40 uM compound concentration; replicate [1]Floatratio
348RFU at 24 sec and 40 uM [2] (40μM**)Value of RFU at 24 sec and 40 uM compound concentration; replicate [2]Floatratio
349RFU at 25 sec and 40 uM [1] (40μM**)Value of RFU at 25 sec and 40 uM compound concentration; replicate [1]Floatratio
350RFU at 25 sec and 40 uM [2] (40μM**)Value of RFU at 25 sec and 40 uM compound concentration; replicate [2]Floatratio
351RFU at 26 sec and 40 uM [1] (40μM**)Value of RFU at 26 sec and 40 uM compound concentration; replicate [1]Floatratio
352RFU at 26 sec and 40 uM [2] (40μM**)Value of RFU at 26 sec and 40 uM compound concentration; replicate [2]Floatratio
353RFU at 27 sec and 40 uM [1] (40μM**)Value of RFU at 27 sec and 40 uM compound concentration; replicate [1]Floatratio
354RFU at 27 sec and 40 uM [2] (40μM**)Value of RFU at 27 sec and 40 uM compound concentration; replicate [2]Floatratio
355RFU at 28 sec and 40 uM [1] (40μM**)Value of RFU at 28 sec and 40 uM compound concentration; replicate [1]Floatratio
356RFU at 28 sec and 40 uM [2] (40μM**)Value of RFU at 28 sec and 40 uM compound concentration; replicate [2]Floatratio
357RFU at 29 sec and 40 uM [1] (40μM**)Value of RFU at 29 sec and 40 uM compound concentration; replicate [1]Floatratio
358RFU at 29 sec and 40 uM [2] (40μM**)Value of RFU at 29 sec and 40 uM compound concentration; replicate [2]Floatratio
359RFU at 30 sec and 40 uM [1] (40μM**)Value of RFU at 30 sec and 40 uM compound concentration; replicate [1]Floatratio
360RFU at 30 sec and 40 uM [2] (40μM**)Value of RFU at 30 sec and 40 uM compound concentration; replicate [2]Floatratio

** Test Concentration.
Additional Information
Grant Number: 5 R21 NS056337-02

Data Table (Concise)
Data Table ( Complete ):     View Active Data    View All Data
Classification
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