Bookmark and Share
BioAssay: AID 651936

Evaluation of reversibility of compound binding to AM2.2-beta2AR cells with Powder Set 3and4

Assay Provider: Jonathan Jarvik, Carnegie Mellon University Screening Center/ PI: UNMCMD/ Larry Sklar ..more
_
   
 Tested Compounds
 Tested Compounds
All(11)
 
 
Active(11)
 
 
 Tested Substances
 Tested Substances
All(12)
 
 
Active(12)
 
 
 Related BioAssays
 Related BioAssays
AID: 651936
Data Source: NMMLSC (UNMCMD_BAFAP_REVERSIBILITY_POWDERSET3AND4)
Depositor Category: NIH Molecular Libraries Probe Production Network
Deposit Date: 2012-12-12
Hold-until Date: 2013-12-11
Modify Date: 2013-12-12

Data Table ( Complete ):           View Active Data    View All Data
Target
Sequence: single chain variable fragment antibody type HL1.1-TO1 [synthetic construct]
Description ..   
Protein Family: Immunoglobulin (Ig) heavy chain (H), variable (V) domain

Related Protein 3D Structures     More BioActivity Data..
BioActive Compounds: 11
Related Experiments
Show more
AIDNameTypeComment
651701Summary of HTS Screening Project for Inhibitors of fluorogen-FAP tag interactionsSummarydepositor-specified cross reference: Summary of HTS Screening Project for Inhibitors of fluorogen-FAP tag interactions
504448Summary of HTS for Non-Canonical Ligands for Beta 2 Adrenergic Receptor InternalizationSummarysame project related to Summary assay
588775Dose response for HTS for Beta-2AR agonists via FAP method from Powderset1Confirmatorysame project related to Summary assay
623882Dose response for HTS for Beta-2AR agonists via FAP method from Powderset2Confirmatorysame project related to Summary assay
623947Dose response for HTS for Beta-2AR agonists via FAP method from Powderset3Confirmatorysame project related to Summary assay
651694Cytotoxicity Dose Response with AM2.2-beta2AR cells for PowderSet01Confirmatorysame project related to Summary assay
651698Counter screen for HTS for Beta-2AR agonists with FAP-tagged mouse CCR5 with Powderset1Confirmatorysame project related to Summary assay
651728Counter screen for HTS for Beta-2AR agonists with FAP-tagged human CCR5 with Powderset2Confirmatorysame project related to Summary assay
651729Counter screen for HTS for Beta-2AR agonists with FAP-tagged GPR32 with Powderset2Confirmatorysame project related to Summary assay
651853Counter screen for HTS for Beta-2AR agonists with FAP-tagged human GPR32 with Powderset3Confirmatorysame project related to Summary assay
651855Counter screen for HTS for Beta-2AR agonists with FAP-tagged human CCR5 with Powderset3Confirmatorysame project related to Summary assay
651870Cytotoxicity Dose Response with AM2.2-beta2AR cells for PowderSet4Confirmatorysame project related to Summary assay
651872Dose response for HTS for Beta-2AR agonists via FAP method from Powderset4Confirmatorysame project related to Summary assay
651873Counter screen for HTS for Beta-2AR agonists with FAP-tagged human CCR5 with Powderset4Confirmatorysame project related to Summary assay
651875Counter screen for HTS for Beta-2AR agonists with FAP-tagged GPR32 with Powderset4Confirmatorysame project related to Summary assay
651912Dose response for HTS for Beta-2AR agonists via FAP method from Powderset5Confirmatorysame project related to Summary assay
651913Counter screen for HTS for Beta-2AR agonists with FAP-tagged human CCR5 with Powderset5Confirmatorysame project related to Summary assay
651914Counter screen for HTS for Beta-2AR agonists with FAP-tagged GPR32 with Powderset5Confirmatorysame project related to Summary assay
651938Fluorogen/soluble FAP binding competition assayOthersame project related to Summary assay
Description:
University of New Mexico Assay Overview:
Assay Support: R03 MH093192-01
Project Title: HTS for Non-Canonical Ligands for Beta 2 Adrenergic Receptor Internalization
Assay Provider: Jonathan Jarvik, Carnegie Mellon University Screening Center/ PI: UNMCMD/ Larry Sklar
Lead Biologist: Yang Wu
Chemistry Center/ PI: Vanderbilt Specialty Chemistry Center/Craig Lindsley Chemistry Center Lead: Shaun Stauffer
Assay Implementation: Yang Wu, Phillip Tapia

Assay Background and Significance:

G protein-coupled receptors represent the largest family of proteins in the human genome with an estimated number of approximately 800. Because of their central involvement in almost every aspect of human physiology, they also represent the largest target for medical intervention [Lin and Civelli, Annu Med 36 (2004), 204-14]. Today, GPCRs represent the target of approximately 30-40% of all drugs on the market. Indeed, of the 50 top-selling drugs in the United States in 2007, 18 target GPCRs, with combined sales of approximately 25 billion dollars.

Of about 800 GPCRs, 500 are chemosensory, representing the chemokine/chemoattractant GPCRs, and the olfactory and gustatory GPCRs. Although the former have been thoroughly characterized for the most part, members of the latter, particularly the olfactory receptors which may include the important category of pheromones, have had only a small number of ligands identified. The remaining GPCRs, approximately 360, constitute the transmitter GPCRs. Of these, approximately 100 receptors still have no known ligand. Such receptors with no known physiological ligand are referred to as orphan receptors and arose from cloning strategies based on limited homology within almost all GPCRs (predominantly during the 1990's) as well as the sequencing of the human genome (in 2000) [Chung et al, Br J Pharmacol 153 Suppl 1 (2008), S339-46].

The search for endogenous ligands for orphan GPCRs has been challenging. This process has given rise to the field of reverse pharmacology, which uses orphan GPCRs to identify novel ligands, which together often lead to the characterization of new physiological paradigms. Over the last 20 years, this approach has led to the deorphanization of about 300 GPCRs. Many of the ligands were already known and their biology characterized, but their receptor was unknown. But in some of these instances, this process also led to the identification of novel transmitters [Civelli et al., Pharmacol Ther 110 (2006), 525-32].

During the 1990's, approximately 10 GPCRs were deorphanized per year. However, very few have been deorphanized since 2004. In addition, no novel transmitters have been identified since that time [Chung et al, Br J Pharmacol 153 Suppl 1 (2008), S339-46]. Given these facts, the question arises as to whether the remaining orphan GPCRs will be readily deorphanized. One major issue is that the pool of known transmitters for which no receptor is known has been essentially depleted. Since all the possible transmitters have now been matched to GPCRs, the current orphan GPCRs can only bind unknown ligands, for which the identification is also slow and resource intense. This is particularly true given that the concentrations of many transmitters in vivo is exceedingly low, making their identification difficult. It is also possible that the expression of some ligands may be developmentally or environmentally regulated.

Almost all current reverse pharmacological/screening approaches rely on monitoring second messenger levels such as calcium mobilization, cAMP production and transcriptional activation. Thus, successful screening requires knowledge of the pathway for a given receptor, in particular the G protein to which the receptor couples. As the number of heterotrimeric G protein combinations is large, this is not a trivial task.

An alternate approach to measuring signal transduction is the monitoring receptor internalization. Virtually all known GPCRs undergo activation-dependent internalization as a mechanism to reduce cell surface receptor numbers. Internalization does not require G protein coupling. Instead, the activity of one of four G protein receptor kinases (GRKs) is required. In most instances, the binding of an accessory protein, arrestin, is also required. One screening approach that has been developed is the recruitment of GFP-tagged arrestin to either the plasma membrane or intracellular endosomes.

The beta-Arrestin clustering assay, developed by Norak Technologies, requires high resolution imaging, well spread, adherent cells, and extensive image analysis to determine the response to a treatment. Other methods, such a receptor desensitization measurements on non-permeabilized cells rely on measurement of subtle changes in intensity. As described below, the CMU TCNP is developing sensors for GPCR responses that are readily compatible with HTS flow cytometry and multiplexing when the GPCRs are expressed in suspension cell lines.
Protocol
1. Spin down AM2.2-beta2 AR cells and resuspend in serum free RPMI media and yielded a final cell density of 5e5/mL.
2. Add 500uL of cells to assay tubes followed by 5muL of 100 x compound at 5 differnt concentration in DMSO or 5muL of DMSO only for NCntrls to each sets of tubes.
3. Mix well by mild vortexing and keep it at 37 degrees C incubator for 90mins.
4. Take 100uL of cells from each set of the cells, add 150nM TO1-2p to the cells and read by the cytometery.
5. Wash the rest of the cells with washing medium (RPMI with 1% of fetal bovine serum), by repeatedly spin down the cells, discard supernatant, and resuspend in fresh washing medium. After 3, 6 and 9 washes, take an aliquot of cells , add 150nM TO1-2p to the cells and read by Accuri flow cytometer immediately.
Calculations:
Median Channel fluorescence are recorded by Accuri C6 flow cytometer (Ann Arbor, MI). Then the baseline were subtracted from these values and the reported normalized binding of fluorescent fluorogen were calculated for the different concentrations of sample compound.
If more sites will be accessible for fluorogen binding when the compound concentration was reduced by repeatedly washing, and the original compound concentration is positively associated with the number of washes required to regain the binding of the fluoresecent fluorogen, the binding of the sample compound is considered reversible.
Keywords: beta2AR, UNMCMD, Fluorogen, Fluorogen activating protein
Categorized Comment - additional comments and annotations
From PubChem:
Assay Cell Type: AM2.2-beta2AR
Result Definitions
Show more
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1Binding after 0 washes with 0.002microM Sample (0.002μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
2Binding after 3 washes with 0.002microM Sample (0.002μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
3Binding after 6 washes with 0.002microM Sample (0.002μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
4Binding after 9 washes with 0.002microM Sample (0.002μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
5Binding after 0 washes with 0.003microM Sample (0.003μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
6Binding after 3 washes with 0.003microM Sample (0.003μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
7Binding after 6 washes with 0.003microM Sample (0.003μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
8Binding after 9 washes with 0.003microM Sample (0.003μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
9Binding after 0 washes with 0.006microM Sample (0.006μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
10Binding after 3 washes with 0.006microM Sample (0.006μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
11Binding after 6 washes with 0.006microM Sample (0.006μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
12Binding after 9 washes with 0.006microM Sample (0.006μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
13Binding after 0 washes with 0.008microM Sample (0.008μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
14Binding after 3 washes with 0.008microM Sample (0.008μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
15Binding after 6 washes with 0.008microM Sample (0.008μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
16Binding after 9 washes with 0.008microM Sample (0.008μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
17Binding after 0 washes with 0.0085microM Sample (0.0085μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
18Binding after 3 washes with 0.0085microM Sample (0.0085μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
19Binding after 6 washes with 0.0085microM Sample (0.0085μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
20Binding after 9 washes with 0.0085microM Sample (0.0085μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
21Binding after 0 washes with 0.01microM Sample (0.01μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
22Binding after 3 washes with 0.01microM Sample (0.01μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
23Binding after 6 washes with 0.01microM Sample (0.01μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
24Binding after 9 washes with 0.01microM Sample (0.01μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
25Binding after 0 washes with 0.014microM Sample (0.014μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
26Binding after 3 washes with 0.014microM Sample (0.014μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
27Binding after 6 washes with 0.014microM Sample (0.014μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
28Binding after 9 washes with 0.014microM Sample (0.014μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
29Binding after 0 washes with 0.02microM Sample (0.02μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
30Binding after 3 washes with 0.02microM Sample (0.02μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
31Binding after 6 washes with 0.02microM Sample (0.02μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
32Binding after 9 washes with 0.02microM Sample (0.02μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
33Binding after 0 washes with 0.028microM Sample (0.028μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
34Binding after 3 washes with 0.028microM Sample (0.028μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
35Binding after 6 washes with 0.028microM Sample (0.028μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
36Binding after 9 washes with 0.028microM Sample (0.028μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
37Binding after 0 washes with 0.03microM Sample (0.03μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
38Binding after 3 washes with 0.03microM Sample (0.03μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
39Binding after 6 washes with 0.03microM Sample (0.03μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
40Binding after 9 washes with 0.03microM Sample (0.03μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
41Binding after 0 washes with 0.042microM Sample (0.042μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
42Binding after 3 washes with 0.042microM Sample (0.042μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
43Binding after 6 washes with 0.042microM Sample (0.042μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
44Binding after 9 washes with 0.042microM Sample (0.042μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
45Binding after 0 washes with 0.06microM Sample (0.06μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
46Binding after 3 washes with 0.06microM Sample (0.06μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
47Binding after 6 washes with 0.06microM Sample (0.06μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
48Binding after 9 washes with 0.06microM Sample (0.06μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
49Binding after 0 washes with 0.08microM Sample (0.08μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
50Binding after 3 washes with 0.08microM Sample (0.08μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
51Binding after 6 washes with 0.08microM Sample (0.08μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
52Binding after 9 washes with 0.08microM Sample (0.08μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
53Binding after 0 washes with 0.085microM Sample (0.085μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
54Binding after 3 washes with 0.085microM Sample (0.085μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
55Binding after 6 washes with 0.085microM Sample (0.085μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
56Binding after 9 washes with 0.085microM Sample (0.085μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
57Binding after 0 washes with 0.1microM Sample (0.1μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
58Binding after 3 washes with 0.1microM Sample (0.1μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
59Binding after 6 washes with 0.1microM Sample (0.1μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
60Binding after 9 washes with 0.1microM Sample (0.1μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
61Binding after 0 washes with 0.14microM Sample (0.14μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
62Binding after 3 washes with 0.14microM Sample (0.14μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
63Binding after 6 washes with 0.14microM Sample (0.14μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
64Binding after 9 washes with 0.14microM Sample (0.14μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
65Binding after 0 washes with 0.2microM Sample (0.2μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
66Binding after 3 washes with 0.2microM Sample (0.2μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
67Binding after 6 washes with 0.2microM Sample (0.2μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
68Binding after 9 washes with 0.2microM Sample (0.2μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
69Binding after 0 washes with 0.28microM Sample (0.28μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
70Binding after 3 washes with 0.28microM Sample (0.28μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
71Binding after 6 washes with 0.28microM Sample (0.28μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
72Binding after 9 washes with 0.28microM Sample (0.28μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
73Binding after 0 washes with 0.3microM Sample (0.3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
74Binding after 3 washes with 0.3microM Sample (0.3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
75Binding after 6 washes with 0.3microM Sample (0.3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
76Binding after 9 washes with 0.3microM Sample (0.3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
77Binding after 0 washes with 0.33microM Sample (0.33μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
78Binding after 3 washes with 0.33microM Sample (0.33μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
79Binding after 6 washes with 0.33microM Sample (0.33μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
80Binding after 9 washes with 0.33microM Sample (0.33μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
81Binding after 0 washes with 0.42microM Sample (0.42μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
82Binding after 3 washes with 0.42microM Sample (0.42μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
83Binding after 6 washes with 0.42microM Sample (0.42μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
84Binding after 9 washes with 0.42microM Sample (0.42μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
85Binding after 0 washes with 0.5microM Sample (0.5μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
86Binding after 3 washes with 0.5microM Sample (0.5μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
87Binding after 6 washes with 0.5microM Sample (0.5μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
88Binding after 9 washes with 0.5microM Sample (0.5μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
89Binding after 0 washes with 0.6microM Sample (0.6μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
90Binding after 3 washes with 0.6microM Sample (0.6μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
91Binding after 6 washes with 0.6microM Sample (0.6μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
92Binding after 9 washes with 0.6microM Sample (0.6μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
93Binding after 0 washes with 0.8microM Sample (0.8μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
94Binding after 3 washes with 0.8microM Sample (0.8μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
95Binding after 6 washes with 0.8microM Sample (0.8μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
96Binding after 9 washes with 0.8microM Sample (0.8μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
97Binding after 0 washes with 0.85microM Sample (0.85μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
98Binding after 3 washes with 0.85microM Sample (0.85μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
99Binding after 6 washes with 0.85microM Sample (0.85μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
100Binding after 9 washes with 0.85microM Sample (0.85μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
101Binding after 0 washes with 1microM Sample (1μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
102Binding after 3 washes with 1microM Sample (1μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
103Binding after 6 washes with 1microM Sample (1μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
104Binding after 9 washes with 1microM Sample (1μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
105Binding after 0 washes with 1.4microM Sample (1.4μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
106Binding after 3 washes with 1.4microM Sample (1.4μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
107Binding after 6 washes with 1.4microM Sample (1.4μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
108Binding after 9 washes with 1.4microM Sample (1.4μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
109Binding after 0 washes with 2microM Sample (2μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
110Binding after 3 washes with 2microM Sample (2μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
111Binding after 6 washes with 2microM Sample (2μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
112Binding after 9 washes with 2microM Sample (2μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
113Binding after 0 washes with 3microM Sample (3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
114Binding after 3 washes with 3microM Sample (3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
115Binding after 6 washes with 3microM Sample (3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
116Binding after 9 washes with 3microM Sample (3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
117Binding after 0 washes with 3.3microM Sample (3.3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
118Binding after 3 washes with 3.3microM Sample (3.3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
119Binding after 6 washes with 3.3microM Sample (3.3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
120Binding after 9 washes with 3.3microM Sample (3.3μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
121Binding after 0 washes with 6microM Sample (6μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
122Binding after 3 washes with 6microM Sample (6μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
123Binding after 6 washes with 6microM Sample (6μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
124Binding after 9 washes with 6microM Sample (6μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
125Binding after 0 washes with 10microM Sample (10μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
126Binding after 3 washes with 10microM Sample (10μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
127Binding after 6 washes with 10microM Sample (10μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
128Binding after 9 washes with 10microM Sample (10μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
129Binding after 0 washes with 20microM Sample (20μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
130Binding after 3 washes with 20microM Sample (20μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
131Binding after 6 washes with 20microM Sample (20μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
132Binding after 9 washes with 20microM Sample (20μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
133Binding after 0 washes with 30microM Sample (30μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
134Binding after 3 washes with 30microM Sample (30μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
135Binding after 6 washes with 30microM Sample (30μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
136Binding after 9 washes with 30microM Sample (30μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
137Binding after 0 washes with 60microM Sample (60μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
138Binding after 3 washes with 60microM Sample (60μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
139Binding after 6 washes with 60microM Sample (60μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
140Binding after 9 washes with 60microM Sample (60μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
141Binding after 0 washes with 100microM Sample (100μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
142Binding after 3 washes with 100microM Sample (100μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
143Binding after 6 washes with 100microM Sample (100μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat
144Binding after 9 washes with 100microM Sample (100μM**)Normalized binding of fluorescent fluorogen after a number of washes with the sample compound presentFloat

** Test Concentration.
Additional Information
Grant Number: R03 MH093192-01

Data Table (Concise)
Data Table ( Complete ):     View Active Data    View All Data
PageFrom: