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BioAssay: AID 651845

Inhibition of T.cruzi proliferation in culture Measured in Cell-Based System Using Plate Reader - 2138-01_Inhibitor_Dose_DryPowder_Activity

Assay Overview: The assay detects intracellular trypanosomes that are replicating inside host cells. NIH3T3 cells are trypsinized, counted, and plated in 384-well tissue culture plates. After plating the NIH3T3 cells, compounds are added to the wells. T. cruzi that are genetically modified to express b-galactosidase are then harvested and active Trypomastigotes form are counted and co-cultured with the NIH3T3 cells. After 90 hours of co-culture, all cells in the well are lysed and b-galactosidase production is detected using a luminescent reporter system (GalScreen). ..more
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 Tested Compounds
 Tested Compounds
All(57)
 
 
Active(27)
 
 
Inactive(2)
 
 
Inconclusive(28)
 
 
 Tested Substances
 Tested Substances
All(58)
 
 
Active(27)
 
 
Inactive(2)
 
 
Inconclusive(29)
 
 
 Related BioAssays
 Related BioAssays
AID: 651845
Data Source: Broad Institute (2138-01_Inhibitor_Dose_DryPowder_Activity)
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Depositor Category: NIH Molecular Libraries Probe Production Network
BioAssay Version:
Deposit Date: 2012-12-04
Hold-until Date: 2013-07-15
Modify Date: 2013-07-16

Data Table ( Complete ):           Active    All
BioActive Compounds: 27
Depositor Specified Assays
AIDNameTypeComment
624280Broad Institute Inhibition of T.cruzi replication in culture Inhibitor Probe ProjectsummarySummary assay
Description:
Keywords: Trypanosoma cruzi, Chagas disease

Assay Overview: The assay detects intracellular trypanosomes that are replicating inside host cells. NIH3T3 cells are trypsinized, counted, and plated in 384-well tissue culture plates. After plating the NIH3T3 cells, compounds are added to the wells. T. cruzi that are genetically modified to express b-galactosidase are then harvested and active Trypomastigotes form are counted and co-cultured with the NIH3T3 cells. After 90 hours of co-culture, all cells in the well are lysed and b-galactosidase production is detected using a luminescent reporter system (GalScreen).

Expected Outcome: Compounds significantly suppressing luminescence, and therefore b-galactosidases expression will be identified as hits in the screen. Compounds that inhibit luminescence activity may kill T. cruzi, inhibit T. cruzi invasion or inhibit development of the parasite within the host cell. Compounds that are toxic to the host cell will be excluded in secondary assays.
Protocol
PROTOCOL: HTS ASSAY in 384-well plate TRYPANOSOMA CRUZI

A. Cells and parasite:
-LLC-MK2 cells (rhesus monkey kidney epithelial cell line): ATCC CCL7.1
-#NIH/3T3 cells (mouse embryonic fibroblast cell line): ATCC CRL-1658
-Trypanosoma cruzi expressing beta-galactosidase (Tc-beta-gal: Tulahuen strain, clone C4 from: Buckner et al., 1996, Antimicrobial agents and chemotherapy, 40:11, pp 2592-2597.): ATCC PRA-330
C. Reagents, materials, solutions and culture media:
Reagents:
-DMEM with Phenol Red, high glucose, with L-glutamine and Sodium pyruvate (Cellgro, cat number: 10-013-CM)
-PSG or Penicillin-streptomycin-L-glutamine (Gibco-Invitrogen, cat number: 10378-016)
-FBS-Heat inactivated fetal bovine serum (Gibco-invitrogen, cat number: 16140-089)
-0.25% Trypsin-EDTA 1X (Gibco-Invitrogen, cat number: 25200-072)
-Sterile PBS (Phosphate Buffer Saline) 1X
-GalScreen, Buffer B (Applied Biosystems, cat number: T1031)
-Horse Serum (Invitrogen, 16050-114)
Materials:
-T225 culture flasks with vented caps (BD Falcon, cat number: 353028)
- Corning Hyperflasks (Corning, cat number: 10024)
Culture medium:
-For cell propagation:
90% DMEM+Phenol red, 10% FBS, 1% PSG. Mix and filter through 0.2 microns membrane. Keep at 4 degrees C. Warm up to 37 degrees C in water bath before use.
-For Tc culture and assays:
98% DMEM+Phenol red, 2% FBS, 10% Horse Serum, 1% PSG. Mix and filter through 0.2 microns membrane. Keep at 4 degrees C. Warm up to 37 degrees C in water bath before use.
98% DMEM+Phenol red, 2% FBS, 1% PSG. Mix and filter through 0.2 microns membrane. Keep at 4 degrees C. Warm up to 37 degrees C in water bath before use.

Solutions:
-GalScreen . Using GalScreen base kit (Applied Biosystems), mix Buffer B (Applied Biosystems, T2361) with 1:25 substrate (Applied Biosystems, T2359) .

D. Parasite and cell culture
Cell culture : NIH/3T3
Notes:
-NIH/3T3 cells are cultivated in DMEM supplemented with 10% FBS and 1% PSG in T225.
-Conditions can be adapted to other culture plate sizes.
-Cells are usually passaged
Protocol:
-Aspirate medium.
-Rinse cells with 10 ml PBS/plate.
-Aspirate PBS.
-Add 4 ml of pre-warmed trypsin-EDTA, swirl the dish to make sure the trypsin covers all the cells.
-Leave the dish a few minutes at room temperature (usually not more than 5).
-Check that the cells are detaching.
-Add 21 ml of medium, pipet up and down to detach all the cells.
-Take 75 ul of media and add to Cellometer cassette (Nexcelom Biosciences). Using the Cellometer Auto T4 (Nexcelom Biosciences), focus so cell bodies have dark edges and clear/white centers. Select NIH3T3 preset menu, dilution 1x, display image, and count. Using this number dilute in either 2% FBS/DMEM for assays or 10% FBS/DMEM for propagation
Cell culture : LLC-MK2
Notes:
-LLC-MK2 cells are cultivated in DMEM +Phenol red supplemented with 10% FBS and 1% PSG in T175 flasks in 35 ml total of medium.
-Conditions can be adapted to other culture plate sizes.
-Cells are usually passaged twice a week at 1:4 to 1:8 ratios.
Protocol:
-Aspirate medium.
-Rinse cells with 10 ml PBS/plate.
-Aspirate PBS.
-Add 5 ml of pre-warmed trypsin-EDTA, swirl the dish to make sure the trypsin covers all the cells.
-Place the dishes back in the incubator for 5 min.
-Check that the cells are detaching.
-Add 20 ml of medium, pipet up and down to detach all the cells. Dilute into 2% FBS/DMEM for assay or 10% FBS/DMEM for propagation.
Parasite culture: Trypanosoma cruzi beta-gal (Tc)
Notes:
-Tc-beta-gal are cultivated in DMEM +Phenol red supplemented with 2% FBS and 1% PSG in T175 flasks with vented caps in 35 ml total of medium.
Protocol:
-The day before infection (or at least 2-3 hours before), plate 2.5 Million LLC-MK2 cells/T175 in DMEM+2% FBS
-Either thaw Tc in from liquid nitrogen stock into 50 ml 2% FBS/DMEM or remove the media from a propagating LLC-MK2 co-culture, in other words, harvesting the medium containing the free Tc in 50 ml falcon tubes.
-Spin 10 min at 2200 rpm
-Aspirate the supernatant until 15 ml is left
-Place back the tubes in the incubator delicately (so as not to disturb the pellet)
-Incubate minimum 4 hours at 37 degrees C.
-Take supernatant to fresh tube.
-To count: Mix 25 ul Tc with 25 ul 16% PFA, mix, put 75 ul Cellometer cassette and count using the Cellometer Auto M10 (Nexcelom Biosciences).
-Wait for 2-5 min to let the Tc settle, press display image, focus, and count. Note, if there are numerous bubbles or low numbers of Tc, the counter will be inaccurate. It is critical to check every screen to see that it has properly counted. Often, there is ~ 10-20% over counting. Alternatively, TC can be counted a hemacytometer using the smaller/inner grids.
Leave Tc incubating in 50 ml conicals until ready to dilute for experiment or inoculate new LLC-MK2 culture.
-Aspirate LLC-MK2 media (used 10% for plating) and replace with 2%+10% Horse Serum FBS/DMEM.
-Plate 5-10 Million on Tc on fresh LLC-MK2
-24 hours later, wash cells with PBS and put fresh media on cells.without horse serum (Use 2% FBS/DMEM). If there are visibile Tc after 3 days, repeat PBS wash and changing of the media.-#Harvest Tc trypomastigotes on Day 7.

E. Growth inhibition assay for HTS in 384 well plates:
Notes:
-Final volume per well for cells+parasites+compound is 50 ul.
-Final volume after adding substrate is ~70 ul. (evaporation of 60ul to 40 ul after 90 hrs, and then 30ul GalScreen addition)
-The assay is performed in DMEM+ 2% FBS and 1% PSG .
-Experiment set up is usually done ~12pm. Assay has to be incubated 90 hrs or a bit more.
Protocol:
-warm up medium 2% FBS/DMEM
-harvest parasites in 50 ml tubes (1 flasks per tube)
-spin 10 min at 2200 rpm
-Aspirate media ~ 15 ml and place them on a rack in the incubator to let trypomastigotes swim out of the pellet for 3-5 hours.
-In the meantime, trypsinize NIH/3T3 cells as described in cell culture protocol
-When NIH3T3 are detached, harvest them in DMEM- 2% FBS and 1% PSG and count using the NIH3T3 program using Cellometer Auto T4 (Nexcelom Biosciences).
-Dilute cells to 166, 667 cells /ml and add to flask.
-plate 5,000 cells/30ul per well using a Thermo MultiDrop Combi liquid dispenser and a sterilized standard sized dispensing cassette adding at a fast speed in a tissue culture hood.
-Put back in incubator for 3 hours to allow cells to attach.
-Count Tc as from protocol above.
-Dilute to 166,667/ml and transfer a 2 liter flask.
-Pin 100 nl compounds/DMSO to each well
-Add shortly after 30 ul/well of parasites (5000 Tc) using a Thermo MultiDrop Combi liquid dispenser and a sterilized standard sized dispensing cassette adding at slow speed.
-Incubate for 4 days (or minimum 90 hours).
-On day of substrate addition, prepare GalScreen(Buffer B with 1:25 dilution substrate ).
-Add 30 ul per well of 384 well plate using a Thermo MultiDrop Combi liquid dispenser and a sterilized standard sized dispensing cassette adding at a fast speed.
-Incubate for 60 minutes
-Read using the ultrasensistive luminescence program on the Envision (Perkin Elmer, 0.1 sec/well).
Comment
PRESENCE OF CONTROLS: Neutral control wells (NC; n=104) and positive control wells (PC; n=10) were included on every plate.

EXPECTED OUTCOME: Active compounds result in decreasing readout signal.
The compounds were assayed in multiple independent instances using an identical protocol; each instance is called a 'test'. For each test, the following analysis was applied:

ACTIVE CONCENTRATION LIMIT:
For each sample, the highest valid tested concentration (Max_Concentration) was determined and the active concentration limit (AC_limit) was set to equal (2)(Max_Concentration).

NORMALIZATION:
The raw signals of the plate wells were normalized using the 'Neutral Controls Minus Inhibitors' method in Genedata Assay Analyzer (v7.0.3):
The median raw signal of the intraplate neutral control wells was set to a normalized activity value of 0.
The median raw signal of the intraplate positive control wells was set to a normalized activity value of -100.
Experimental wells values were scaled to this range.

PATTERN CORRECTION: No plate pattern correction algorithm from Genedata Condoseo (v.7.0.3) was applied.

MEASUREMENT USED TO DETERMINE ACTIVE CONCENTRATION (AC): AC50
AC values were calculated using the curve fitting strategies in Genedata Screener Condoseo (7.0.3).
AC values were calculated up to the active concentration limit described for each sample.
pAC was set to equal -1*log10(AC)

PUBCHEM_ACTIVITY_OUTCOME:
Activity_Outcome = 1 (inactive) when:
a) compound shows activity but in a direction opposite to the expected outcome
in these cases, values describing curve fitting parameters (Sinf, S0, Hill Slope, log_AC50, log_AC50_SE) are set to null
b) curve fit is constant where activity is > -30% and < 30% at all tested concentrations, or
c) AC > AC_limit
Activity_Outcome = 2 (active) when:
AC <= AC_limit
Activity_Outcome = 3 (inconclusive) when:
a) Curve fitting strategy resulted in a constant fit with activity >= -70% but <= -30%, or
b) The fit was deemed not valid due to poor fit quality.

PUBCHEM_ACTIVITY_SCORE:
If PUBCHEM_ACTIVITY_OUTCOME = 1 (inactive) or 3 (inconclusive),
then PUBCHEM_ACTIVITY_SCORE = 0
If PUBCHEM_ACTIVITY_OUTCOME = 2 (active)
then PUBCHEM_ACTIVITY_SCORE = (10)(pAC)
Scores relate to AC in this manner:
120 = 1 pM
90 = 1 nM
60 = 1 uM
30 = 1 mM
0 = 1 M
When the active concentration (AC) is calculated to be greater than the highest valid tested concentration (Max_Concentration), the PUBCHEM_ACTIVITY_SCORE is calculated using Max_Concentration as the basis.
When the active concentration (AC) is calculated to be less than the lowest tested concentration, the PUBCHEM_ACTIVITY_SCORE is calculated using the lowest tested concentration as the basis.

DATA AGGREGATION:
Once the data for each test was processed, the test number was appended to all column headers in that test's data set. The individual test results were then aggregated as follows:

1. The final PUBCHEM_ACTIVITY_OUTCOME was set to:
a. '2' (active) when all test outcomes were '2' (active), or
b. '1' (inactive) when all test outcomes were '1' (inactive), or
c. '3' (inconclusive) when the test outcomes were mixed.

2. The final ACTIVE_CONCENTRATION (AC) was set as follows:
a. If the final PUBCHEM_ACTIVITY_OUTCOME = 2, AC was set as the mean of the constituent test active concentrations;
b. If the final PUBCHEM_ACTIVITY_OUTCOME = 1 or 3, AC was left empty.

3. The final PUBCHEM_ACTIVITY_SCORE was calculated based on the aggregated ACTIVE_CONCENTRATION, using the same logic described above for individual test scores.

Note:
The individual dose data point columns ('Activity_at_xxuM') reported here represent the median of valid (unmasked) replicate observations at each concentration. These values are the inputs to a curve fitting algorithm.
All other data columns represent values which are derived during the curve fitting algorithm; this may sometimes include automatic further masking of some replicate data points.
Occasionally this results in perceived inconsistencies: for example, between the derived 'Maximal_Activity' and the apparent most active data point.
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1AC50_Qualifier'>','=', or '<'String
2AC50_uM*The concentration at which activity reaches 50% of the maximumFloatμM
3pAC50_MEqual to -1*log10(AC50)String
4ACTIVITY_OUTCOME_TEST1The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
5ACTIVITY_SCORE_TEST1The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
6ASSAYDATA_COMMENT_TEST1Comments relevant to the subset of data included in the indicated Test number.String
7AC50_Qualifier_TEST1'>','=', or '<'String
8AC50_uM_TEST1The concentration at which activity reaches 50% of the maximumFloatμM
9pAC50_M_TEST1Equal to -1*log10(AC50)String
10Hill_Slope_TEST1The slope at AC50Float
11S0_(%)_TEST1The fitted activity value at zero concentrationFloat%
12Sinf_(%)_TEST1The fitted activity value at infinite concentrationFloat%
13Num_Points_TEST1The number of data points used to generate the plotInteger
14Max_Activity_(%)_TEST1The maximum activity value observed, based on mean of replicates per concentrationFloat%
15Max_Activity_Conc_uM_TEST1The concentration at which the maximum activity is observedFloatμM
16Max_Concentration_uM_TEST1Maximum valid test concentrationFloatμM
17Activity_at_0uM_(%)_TEST1 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
18Activity_at_0.000001uM_(%)_TEST1 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
19Activity_at_0.000003uM_(%)_TEST1 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
20Activity_at_0.000010uM_(%)_TEST1 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
21Activity_at_0.000030uM_(%)_TEST1 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
22Activity_at_0.000090uM_(%)_TEST1 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
23Activity_at_0.000285uM_(%)_TEST1 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
24Activity_at_0.0008uM_(%)_TEST1 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
25Activity_at_0.0009uM_(%)_TEST1 (0.0009μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
26Activity_at_0.0026uM_(%)_TEST1 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
27Activity_at_0.0075uM_(%)_TEST1 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
28Activity_at_0.0235uM_(%)_TEST1 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
29Activity_at_0.068uM_(%)_TEST1 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
30Activity_at_0.21uM_(%)_TEST1 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
31Activity_at_0.6uM_(%)_TEST1 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
32Activity_at_1.8uM_(%)_TEST1 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
33Activity_at_5.6uM_(%)_TEST1 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
34Activity_at_16uM_(%)_TEST1 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
35ACTIVITY_OUTCOME_TEST2The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
36ACTIVITY_SCORE_TEST2The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
37ASSAYDATA_COMMENT_TEST2Comments relevant to the subset of data included in the indicated Test number.String
38AC50_Qualifier_TEST2'>','=', or '<'String
39AC50_uM_TEST2The concentration at which activity reaches 50% of the maximumFloatμM
40pAC50_M_TEST2Equal to -1*log10(AC50)String
41Hill_Slope_TEST2The slope at AC50Float
42S0_(%)_TEST2The fitted activity value at zero concentrationFloat%
43Sinf_(%)_TEST2The fitted activity value at infinite concentrationFloat%
44Num_Points_TEST2The number of data points used to generate the plotInteger
45Max_Activity_(%)_TEST2The maximum activity value observed, based on mean of replicates per concentrationFloat%
46Max_Activity_Conc_uM_TEST2The concentration at which the maximum activity is observedFloatμM
47Max_Concentration_uM_TEST2Maximum valid test concentrationFloatμM
48Activity_at_0uM_(%)_TEST2 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
49Activity_at_0.000001uM_(%)_TEST2 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
50Activity_at_0.000003uM_(%)_TEST2 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
51Activity_at_0.000010uM_(%)_TEST2 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
52Activity_at_0.000030uM_(%)_TEST2 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
53Activity_at_0.000090uM_(%)_TEST2 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
54Activity_at_0.000285uM_(%)_TEST2 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
55Activity_at_0.0008uM_(%)_TEST2 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
56Activity_at_0.0026uM_(%)_TEST2 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
57Activity_at_0.0075uM_(%)_TEST2 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
58Activity_at_0.0235uM_(%)_TEST2 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
59Activity_at_0.068uM_(%)_TEST2 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
60Activity_at_0.21uM_(%)_TEST2 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
61Activity_at_0.6uM_(%)_TEST2 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
62Activity_at_1.8uM_(%)_TEST2 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
63Activity_at_5.6uM_(%)_TEST2 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
64Activity_at_16uM_(%)_TEST2 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
65ACTIVITY_OUTCOME_TEST3The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
66ACTIVITY_SCORE_TEST3The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
67ASSAYDATA_COMMENT_TEST3Comments relevant to the subset of data included in the indicated Test number.String
68AC50_Qualifier_TEST3'>','=', or '<'String
69AC50_uM_TEST3The concentration at which activity reaches 50% of the maximumFloatμM
70pAC50_M_TEST3Equal to -1*log10(AC50)String
71Hill_Slope_TEST3The slope at AC50Float
72S0_(%)_TEST3The fitted activity value at zero concentrationFloat%
73Sinf_(%)_TEST3The fitted activity value at infinite concentrationFloat%
74Num_Points_TEST3The number of data points used to generate the plotInteger
75Max_Activity_(%)_TEST3The maximum activity value observed, based on mean of replicates per concentrationFloat%
76Max_Activity_Conc_uM_TEST3The concentration at which the maximum activity is observedFloatμM
77Max_Concentration_uM_TEST3Maximum valid test concentrationFloatμM
78Activity_at_0uM_(%)_TEST3 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
79Activity_at_0.000001uM_(%)_TEST3 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
80Activity_at_0.000003uM_(%)_TEST3 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
81Activity_at_0.000010uM_(%)_TEST3 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
82Activity_at_0.000030uM_(%)_TEST3 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
83Activity_at_0.000090uM_(%)_TEST3 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
84Activity_at_0.000285uM_(%)_TEST3 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
85Activity_at_0.0008uM_(%)_TEST3 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
86Activity_at_0.0026uM_(%)_TEST3 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
87Activity_at_0.0075uM_(%)_TEST3 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
88Activity_at_0.0235uM_(%)_TEST3 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
89Activity_at_0.068uM_(%)_TEST3 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
90Activity_at_0.21uM_(%)_TEST3 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
91Activity_at_0.6uM_(%)_TEST3 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
92Activity_at_1.8uM_(%)_TEST3 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
93Activity_at_5.6uM_(%)_TEST3 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
94Activity_at_16uM_(%)_TEST3 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
95ACTIVITY_OUTCOME_TEST4The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
96ACTIVITY_SCORE_TEST4The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
97ASSAYDATA_COMMENT_TEST4Comments relevant to the subset of data included in the indicated Test number.String
98AC50_Qualifier_TEST4'>','=', or '<'String
99AC50_uM_TEST4The concentration at which activity reaches 50% of the maximumFloatμM
100pAC50_M_TEST4Equal to -1*log10(AC50)String
101Hill_Slope_TEST4The slope at AC50Float
102S0_(%)_TEST4The fitted activity value at zero concentrationFloat%
103Sinf_(%)_TEST4The fitted activity value at infinite concentrationFloat%
104Num_Points_TEST4The number of data points used to generate the plotInteger
105Max_Activity_(%)_TEST4The maximum activity value observed, based on mean of replicates per concentrationFloat%
106Max_Activity_Conc_uM_TEST4The concentration at which the maximum activity is observedFloatμM
107Max_Concentration_uM_TEST4Maximum valid test concentrationFloatμM
108Activity_at_0uM_(%)_TEST4 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
109Activity_at_0.000001uM_(%)_TEST4 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
110Activity_at_0.000003uM_(%)_TEST4 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
111Activity_at_0.000010uM_(%)_TEST4 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
112Activity_at_0.000030uM_(%)_TEST4 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
113Activity_at_0.000090uM_(%)_TEST4 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
114Activity_at_0.000285uM_(%)_TEST4 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
115Activity_at_0.0008uM_(%)_TEST4 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
116Activity_at_0.0026uM_(%)_TEST4 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
117Activity_at_0.0075uM_(%)_TEST4 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
118Activity_at_0.0235uM_(%)_TEST4 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
119Activity_at_0.068uM_(%)_TEST4 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
120Activity_at_0.21uM_(%)_TEST4 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
121Activity_at_0.6uM_(%)_TEST4 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
122Activity_at_1.8uM_(%)_TEST4 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
123Activity_at_5.6uM_(%)_TEST4 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
124Activity_at_16uM_(%)_TEST4 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
125ACTIVITY_OUTCOME_TEST5The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
126ACTIVITY_SCORE_TEST5The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
127ASSAYDATA_COMMENT_TEST5Comments relevant to the subset of data included in the indicated Test number.String
128AC50_Qualifier_TEST5'>','=', or '<'String
129AC50_uM_TEST5The concentration at which activity reaches 50% of the maximumFloatμM
130pAC50_M_TEST5Equal to -1*log10(AC50)String
131Hill_Slope_TEST5The slope at AC50Float
132S0_(%)_TEST5The fitted activity value at zero concentrationFloat%
133Sinf_(%)_TEST5The fitted activity value at infinite concentrationFloat%
134Num_Points_TEST5The number of data points used to generate the plotInteger
135Max_Activity_(%)_TEST5The maximum activity value observed, based on mean of replicates per concentrationFloat%
136Max_Activity_Conc_uM_TEST5The concentration at which the maximum activity is observedFloatμM
137Max_Concentration_uM_TEST5Maximum valid test concentrationFloatμM
138Activity_at_0uM_(%)_TEST5 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
139Activity_at_0.000001uM_(%)_TEST5 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
140Activity_at_0.000003uM_(%)_TEST5 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
141Activity_at_0.000010uM_(%)_TEST5 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
142Activity_at_0.000030uM_(%)_TEST5 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
143Activity_at_0.000090uM_(%)_TEST5 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
144Activity_at_0.000285uM_(%)_TEST5 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
145Activity_at_0.0008uM_(%)_TEST5 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
146Activity_at_0.0026uM_(%)_TEST5 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
147Activity_at_0.0075uM_(%)_TEST5 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
148Activity_at_0.0235uM_(%)_TEST5 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
149Activity_at_0.068uM_(%)_TEST5 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
150Activity_at_0.21uM_(%)_TEST5 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
151Activity_at_0.6uM_(%)_TEST5 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
152Activity_at_1.8uM_(%)_TEST5 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
153Activity_at_5.6uM_(%)_TEST5 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
154Activity_at_16uM_(%)_TEST5 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
155ACTIVITY_OUTCOME_TEST6The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
156ACTIVITY_SCORE_TEST6The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
157ASSAYDATA_COMMENT_TEST6Comments relevant to the subset of data included in the indicated Test number.String
158AC50_Qualifier_TEST6'>','=', or '<'String
159AC50_uM_TEST6The concentration at which activity reaches 50% of the maximumFloatμM
160pAC50_M_TEST6Equal to -1*log10(AC50)String
161Hill_Slope_TEST6The slope at AC50Float
162S0_(%)_TEST6The fitted activity value at zero concentrationFloat%
163Sinf_(%)_TEST6The fitted activity value at infinite concentrationFloat%
164Num_Points_TEST6The number of data points used to generate the plotInteger
165Max_Activity_(%)_TEST6The maximum activity value observed, based on mean of replicates per concentrationFloat%
166Max_Activity_Conc_uM_TEST6The concentration at which the maximum activity is observedFloatμM
167Max_Concentration_uM_TEST6Maximum valid test concentrationFloatμM
168Activity_at_0uM_(%)_TEST6 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
169Activity_at_0.000001uM_(%)_TEST6 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
170Activity_at_0.000003uM_(%)_TEST6 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
171Activity_at_0.000010uM_(%)_TEST6 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
172Activity_at_0.000030uM_(%)_TEST6 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
173Activity_at_0.000090uM_(%)_TEST6 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
174Activity_at_0.000285uM_(%)_TEST6 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
175Activity_at_0.0008uM_(%)_TEST6 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
176Activity_at_0.0026uM_(%)_TEST6 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
177Activity_at_0.0075uM_(%)_TEST6 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
178Activity_at_0.0235uM_(%)_TEST6 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
179Activity_at_0.068uM_(%)_TEST6 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
180Activity_at_0.21uM_(%)_TEST6 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
181Activity_at_0.6uM_(%)_TEST6 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
182Activity_at_1.8uM_(%)_TEST6 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
183Activity_at_5.6uM_(%)_TEST6 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
184Activity_at_16uM_(%)_TEST6 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
185ACTIVITY_OUTCOME_TEST7The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
186ACTIVITY_SCORE_TEST7The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
187ASSAYDATA_COMMENT_TEST7Comments relevant to the subset of data included in the indicated Test number.String
188AC50_Qualifier_TEST7'>','=', or '<'String
189AC50_uM_TEST7The concentration at which activity reaches 50% of the maximumFloatμM
190pAC50_M_TEST7Equal to -1*log10(AC50)String
191Hill_Slope_TEST7The slope at AC50Float
192S0_(%)_TEST7The fitted activity value at zero concentrationFloat%
193Sinf_(%)_TEST7The fitted activity value at infinite concentrationFloat%
194Num_Points_TEST7The number of data points used to generate the plotInteger
195Max_Activity_(%)_TEST7The maximum activity value observed, based on mean of replicates per concentrationFloat%
196Max_Activity_Conc_uM_TEST7The concentration at which the maximum activity is observedFloatμM
197Max_Concentration_uM_TEST7Maximum valid test concentrationFloatμM
198Activity_at_0uM_(%)_TEST7 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
199Activity_at_0.000001uM_(%)_TEST7 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
200Activity_at_0.000003uM_(%)_TEST7 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
201Activity_at_0.000010uM_(%)_TEST7 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
202Activity_at_0.000030uM_(%)_TEST7 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
203Activity_at_0.000090uM_(%)_TEST7 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
204Activity_at_0.000285uM_(%)_TEST7 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
205Activity_at_0.0008uM_(%)_TEST7 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
206Activity_at_0.0026uM_(%)_TEST7 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
207Activity_at_0.0075uM_(%)_TEST7 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
208Activity_at_0.0235uM_(%)_TEST7 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
209Activity_at_0.068uM_(%)_TEST7 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
210Activity_at_0.21uM_(%)_TEST7 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
211Activity_at_0.6uM_(%)_TEST7 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
212Activity_at_1.8uM_(%)_TEST7 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
213Activity_at_5.6uM_(%)_TEST7 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
214Activity_at_16uM_(%)_TEST7 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
215ACTIVITY_OUTCOME_TEST8The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
216ACTIVITY_SCORE_TEST8The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
217ASSAYDATA_COMMENT_TEST8Comments relevant to the subset of data included in the indicated Test number.String
218AC50_Qualifier_TEST8'>','=', or '<'String
219AC50_uM_TEST8The concentration at which activity reaches 50% of the maximumFloatμM
220pAC50_M_TEST8Equal to -1*log10(AC50)String
221Hill_Slope_TEST8The slope at AC50Float
222S0_(%)_TEST8The fitted activity value at zero concentrationFloat%
223Sinf_(%)_TEST8The fitted activity value at infinite concentrationFloat%
224Num_Points_TEST8The number of data points used to generate the plotInteger
225Max_Activity_(%)_TEST8The maximum activity value observed, based on mean of replicates per concentrationFloat%
226Max_Activity_Conc_uM_TEST8The concentration at which the maximum activity is observedFloatμM
227Max_Concentration_uM_TEST8Maximum valid test concentrationFloatμM
228Activity_at_0uM_(%)_TEST8 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
229Activity_at_0.000001uM_(%)_TEST8 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
230Activity_at_0.000003uM_(%)_TEST8 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
231Activity_at_0.000010uM_(%)_TEST8 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
232Activity_at_0.000030uM_(%)_TEST8 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
233Activity_at_0.000090uM_(%)_TEST8 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
234Activity_at_0.000285uM_(%)_TEST8 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
235Activity_at_0.0008uM_(%)_TEST8 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
236Activity_at_0.0026uM_(%)_TEST8 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
237Activity_at_0.0075uM_(%)_TEST8 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
238Activity_at_0.0235uM_(%)_TEST8 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
239Activity_at_0.068uM_(%)_TEST8 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
240Activity_at_0.21uM_(%)_TEST8 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
241Activity_at_0.6uM_(%)_TEST8 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
242Activity_at_1.8uM_(%)_TEST8 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
243Activity_at_5.6uM_(%)_TEST8 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
244Activity_at_16uM_(%)_TEST8 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
245ACTIVITY_OUTCOME_TEST9The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
246ACTIVITY_SCORE_TEST9The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
247ASSAYDATA_COMMENT_TEST9Comments relevant to the subset of data included in the indicated Test number.String
248AC50_Qualifier_TEST9'>','=', or '<'String
249AC50_uM_TEST9The concentration at which activity reaches 50% of the maximumFloatμM
250pAC50_M_TEST9Equal to -1*log10(AC50)String
251Hill_Slope_TEST9The slope at AC50Float
252S0_(%)_TEST9The fitted activity value at zero concentrationFloat%
253Sinf_(%)_TEST9The fitted activity value at infinite concentrationFloat%
254Num_Points_TEST9The number of data points used to generate the plotInteger
255Max_Activity_(%)_TEST9The maximum activity value observed, based on mean of replicates per concentrationFloat%
256Max_Activity_Conc_uM_TEST9The concentration at which the maximum activity is observedFloatμM
257Max_Concentration_uM_TEST9Maximum valid test concentrationFloatμM
258Activity_at_0uM_(%)_TEST9 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
259Activity_at_0.000001uM_(%)_TEST9 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
260Activity_at_0.000003uM_(%)_TEST9 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
261Activity_at_0.000010uM_(%)_TEST9 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
262Activity_at_0.000030uM_(%)_TEST9 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
263Activity_at_0.000090uM_(%)_TEST9 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
264Activity_at_0.000285uM_(%)_TEST9 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
265Activity_at_0.0008uM_(%)_TEST9 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
266Activity_at_0.0026uM_(%)_TEST9 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
267Activity_at_0.0075uM_(%)_TEST9 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
268Activity_at_0.0235uM_(%)_TEST9 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
269Activity_at_0.068uM_(%)_TEST9 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
270Activity_at_0.21uM_(%)_TEST9 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
271Activity_at_0.6uM_(%)_TEST9 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
272Activity_at_1.8uM_(%)_TEST9 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
273Activity_at_5.6uM_(%)_TEST9 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
274Activity_at_16uM_(%)_TEST9 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
275ACTIVITY_OUTCOME_TEST10The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
276ACTIVITY_SCORE_TEST10The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
277ASSAYDATA_COMMENT_TEST10Comments relevant to the subset of data included in the indicated Test number.String
278AC50_Qualifier_TEST10'>','=', or '<'String
279AC50_uM_TEST10The concentration at which activity reaches 50% of the maximumFloatμM
280pAC50_M_TEST10Equal to -1*log10(AC50)String
281Hill_Slope_TEST10The slope at AC50Float
282S0_(%)_TEST10The fitted activity value at zero concentrationFloat%
283Sinf_(%)_TEST10The fitted activity value at infinite concentrationFloat%
284Num_Points_TEST10The number of data points used to generate the plotInteger
285Max_Activity_(%)_TEST10The maximum activity value observed, based on mean of replicates per concentrationFloat%
286Max_Activity_Conc_uM_TEST10The concentration at which the maximum activity is observedFloatμM
287Max_Concentration_uM_TEST10Maximum valid test concentrationFloatμM
288Activity_at_0uM_(%)_TEST10 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
289Activity_at_0.000001uM_(%)_TEST10 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
290Activity_at_0.000003uM_(%)_TEST10 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
291Activity_at_0.000010uM_(%)_TEST10 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
292Activity_at_0.000030uM_(%)_TEST10 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
293Activity_at_0.000090uM_(%)_TEST10 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
294Activity_at_0.000285uM_(%)_TEST10 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
295Activity_at_0.0008uM_(%)_TEST10 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
296Activity_at_0.0026uM_(%)_TEST10 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
297Activity_at_0.0075uM_(%)_TEST10 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
298Activity_at_0.0235uM_(%)_TEST10 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
299Activity_at_0.068uM_(%)_TEST10 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
300Activity_at_0.21uM_(%)_TEST10 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
301Activity_at_0.6uM_(%)_TEST10 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
302Activity_at_1.8uM_(%)_TEST10 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
303Activity_at_5.6uM_(%)_TEST10 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
304Activity_at_16uM_(%)_TEST10 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
305ACTIVITY_OUTCOME_TEST11The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
306ACTIVITY_SCORE_TEST11The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
307ASSAYDATA_COMMENT_TEST11Comments relevant to the subset of data included in the indicated Test number.String
308AC50_Qualifier_TEST11'>','=', or '<'String
309AC50_uM_TEST11The concentration at which activity reaches 50% of the maximumFloatμM
310pAC50_M_TEST11Equal to -1*log10(AC50)String
311Hill_Slope_TEST11The slope at AC50Float
312S0_(%)_TEST11The fitted activity value at zero concentrationFloat%
313Sinf_(%)_TEST11The fitted activity value at infinite concentrationFloat%
314Num_Points_TEST11The number of data points used to generate the plotInteger
315Max_Activity_(%)_TEST11The maximum activity value observed, based on mean of replicates per concentrationFloat%
316Max_Activity_Conc_uM_TEST11The concentration at which the maximum activity is observedFloatμM
317Max_Concentration_uM_TEST11Maximum valid test concentrationFloatμM
318Activity_at_0uM_(%)_TEST11 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
319Activity_at_0.000001uM_(%)_TEST11 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
320Activity_at_0.000003uM_(%)_TEST11 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
321Activity_at_0.000010uM_(%)_TEST11 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
322Activity_at_0.000030uM_(%)_TEST11 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
323Activity_at_0.000090uM_(%)_TEST11 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
324Activity_at_0.000285uM_(%)_TEST11 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
325Activity_at_0.0008uM_(%)_TEST11 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
326Activity_at_0.0026uM_(%)_TEST11 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
327Activity_at_0.0075uM_(%)_TEST11 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
328Activity_at_0.0235uM_(%)_TEST11 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
329Activity_at_0.068uM_(%)_TEST11 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
330Activity_at_0.21uM_(%)_TEST11 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
331Activity_at_0.6uM_(%)_TEST11 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
332Activity_at_1.8uM_(%)_TEST11 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
333Activity_at_5.6uM_(%)_TEST11 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
334Activity_at_16uM_(%)_TEST11 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
335ACTIVITY_OUTCOME_TEST12The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
336ACTIVITY_SCORE_TEST12The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
337ASSAYDATA_COMMENT_TEST12Comments relevant to the subset of data included in the indicated Test number.String
338AC50_Qualifier_TEST12'>','=', or '<'String
339AC50_uM_TEST12The concentration at which activity reaches 50% of the maximumFloatμM
340pAC50_M_TEST12Equal to -1*log10(AC50)String
341Hill_Slope_TEST12The slope at AC50Float
342S0_(%)_TEST12The fitted activity value at zero concentrationFloat%
343Sinf_(%)_TEST12The fitted activity value at infinite concentrationFloat%
344Num_Points_TEST12The number of data points used to generate the plotInteger
345Max_Activity_(%)_TEST12The maximum activity value observed, based on mean of replicates per concentrationFloat%
346Max_Activity_Conc_uM_TEST12The concentration at which the maximum activity is observedFloatμM
347Max_Concentration_uM_TEST12Maximum valid test concentrationFloatμM
348Activity_at_0uM_(%)_TEST12 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
349Activity_at_0.000001uM_(%)_TEST12 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
350Activity_at_0.000003uM_(%)_TEST12 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
351Activity_at_0.000010uM_(%)_TEST12 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
352Activity_at_0.000030uM_(%)_TEST12 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
353Activity_at_0.000090uM_(%)_TEST12 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
354Activity_at_0.000285uM_(%)_TEST12 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
355Activity_at_0.0008uM_(%)_TEST12 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
356Activity_at_0.0009uM_(%)_TEST12 (0.0009μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
357Activity_at_0.0026uM_(%)_TEST12 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
358Activity_at_0.0075uM_(%)_TEST12 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
359Activity_at_0.0235uM_(%)_TEST12 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
360Activity_at_0.068uM_(%)_TEST12 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
361Activity_at_0.21uM_(%)_TEST12 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
362Activity_at_0.6uM_(%)_TEST12 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
363Activity_at_1.8uM_(%)_TEST12 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
364Activity_at_5.6uM_(%)_TEST12 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
365Activity_at_16uM_(%)_TEST12 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
366ACTIVITY_OUTCOME_TEST13The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
367ACTIVITY_SCORE_TEST13The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
368ASSAYDATA_COMMENT_TEST13Comments relevant to the subset of data included in the indicated Test number.String
369AC50_Qualifier_TEST13'>','=', or '<'String
370AC50_uM_TEST13The concentration at which activity reaches 50% of the maximumFloatμM
371pAC50_M_TEST13Equal to -1*log10(AC50)String
372Hill_Slope_TEST13The slope at AC50Float
373S0_(%)_TEST13The fitted activity value at zero concentrationFloat%
374Sinf_(%)_TEST13The fitted activity value at infinite concentrationFloat%
375Num_Points_TEST13The number of data points used to generate the plotInteger
376Max_Activity_(%)_TEST13The maximum activity value observed, based on mean of replicates per concentrationFloat%
377Max_Activity_Conc_uM_TEST13The concentration at which the maximum activity is observedFloatμM
378Max_Concentration_uM_TEST13Maximum valid test concentrationFloatμM
379Activity_at_0uM_(%)_TEST13[1] (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
380Activity_at_0uM_(%)_TEST13[2] (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
381Activity_at_0uM_(%)_TEST13[3] (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
382Activity_at_0uM_(%)_TEST13[4] (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
383Activity_at_0.000001uM_(%)_TEST13 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
384Activity_at_0.000003uM_(%)_TEST13 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
385Activity_at_0.000010uM_(%)_TEST13 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
386Activity_at_0.000011uM_(%)_TEST13 (1.1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
387Activity_at_0.000030uM_(%)_TEST13 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
388Activity_at_0.000090uM_(%)_TEST13 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
389Activity_at_0.0001uM_(%)_TEST13 (0.0001μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
390Activity_at_0.000285uM_(%)_TEST13 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
391Activity_at_0.0008uM_(%)_TEST13 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
392Activity_at_0.0009uM_(%)_TEST13 (0.0009μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
393Activity_at_0.0026uM_(%)_TEST13 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
394Activity_at_0.0075uM_(%)_TEST13 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
395Activity_at_0.008uM_(%)_TEST13 (0.008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
396Activity_at_0.0235uM_(%)_TEST13 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
397Activity_at_0.068uM_(%)_TEST13 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
398Activity_at_0.21uM_(%)_TEST13 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
399Activity_at_0.6uM_(%)_TEST13 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
400Activity_at_1.8uM_(%)_TEST13 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
401Activity_at_1.95uM_(%)_TEST13 (1.95μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
402Activity_at_5.6uM_(%)_TEST13 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
403Activity_at_16uM_(%)_TEST13 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
404Activity_at_18uM_(%)_TEST13 (18μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
405ACTIVITY_OUTCOME_TEST14The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
406ACTIVITY_SCORE_TEST14The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
407ASSAYDATA_COMMENT_TEST14Comments relevant to the subset of data included in the indicated Test number.String
408AC50_Qualifier_TEST14'>','=', or '<'String
409AC50_uM_TEST14The concentration at which activity reaches 50% of the maximumFloatμM
410pAC50_M_TEST14Equal to -1*log10(AC50)String
411Hill_Slope_TEST14The slope at AC50Float
412S0_(%)_TEST14The fitted activity value at zero concentrationFloat%
413Sinf_(%)_TEST14The fitted activity value at infinite concentrationFloat%
414Num_Points_TEST14The number of data points used to generate the plotInteger
415Max_Activity_(%)_TEST14The maximum activity value observed, based on mean of replicates per concentrationFloat%
416Max_Activity_Conc_uM_TEST14The concentration at which the maximum activity is observedFloatμM
417Max_Concentration_uM_TEST14Maximum valid test concentrationFloatμM
418Activity_at_0uM_(%)_TEST14 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
419Activity_at_0.000001uM_(%)_TEST14 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
420Activity_at_0.000003uM_(%)_TEST14 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
421Activity_at_0.000010uM_(%)_TEST14 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
422Activity_at_0.000030uM_(%)_TEST14 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
423Activity_at_0.000090uM_(%)_TEST14 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
424Activity_at_0.000285uM_(%)_TEST14 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
425Activity_at_0.0008uM_(%)_TEST14 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
426Activity_at_0.0026uM_(%)_TEST14 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
427Activity_at_0.0075uM_(%)_TEST14 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
428Activity_at_0.0235uM_(%)_TEST14 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
429Activity_at_0.068uM_(%)_TEST14 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
430Activity_at_0.21uM_(%)_TEST14 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
431Activity_at_0.6uM_(%)_TEST14 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
432Activity_at_1.8uM_(%)_TEST14 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
433Activity_at_5.6uM_(%)_TEST14 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
434Activity_at_16uM_(%)_TEST14 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
435ACTIVITY_OUTCOME_TEST15The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
436ACTIVITY_SCORE_TEST15The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
437ASSAYDATA_COMMENT_TEST15Comments relevant to the subset of data included in the indicated Test number.String
438AC50_Qualifier_TEST15'>','=', or '<'String
439AC50_uM_TEST15The concentration at which activity reaches 50% of the maximumFloatμM
440pAC50_M_TEST15Equal to -1*log10(AC50)String
441Hill_Slope_TEST15The slope at AC50Float
442S0_(%)_TEST15The fitted activity value at zero concentrationFloat%
443Sinf_(%)_TEST15The fitted activity value at infinite concentrationFloat%
444Num_Points_TEST15The number of data points used to generate the plotInteger
445Max_Activity_(%)_TEST15The maximum activity value observed, based on mean of replicates per concentrationFloat%
446Max_Activity_Conc_uM_TEST15The concentration at which the maximum activity is observedFloatμM
447Max_Concentration_uM_TEST15Maximum valid test concentrationFloatμM
448Activity_at_0uM_(%)_TEST15 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
449Activity_at_0.000001uM_(%)_TEST15 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
450Activity_at_0.000003uM_(%)_TEST15 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
451Activity_at_0.000010uM_(%)_TEST15 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
452Activity_at_0.000030uM_(%)_TEST15 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
453Activity_at_0.000090uM_(%)_TEST15 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
454Activity_at_0.000285uM_(%)_TEST15 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
455Activity_at_0.0008uM_(%)_TEST15 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
456Activity_at_0.0026uM_(%)_TEST15 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
457Activity_at_0.0075uM_(%)_TEST15 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
458Activity_at_0.0235uM_(%)_TEST15 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
459Activity_at_0.068uM_(%)_TEST15 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
460Activity_at_0.21uM_(%)_TEST15 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
461Activity_at_0.6uM_(%)_TEST15 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
462Activity_at_1.8uM_(%)_TEST15 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
463Activity_at_5.6uM_(%)_TEST15 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
464Activity_at_16uM_(%)_TEST15 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
465ACTIVITY_OUTCOME_TEST16The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
466ACTIVITY_SCORE_TEST16The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
467ASSAYDATA_COMMENT_TEST16Comments relevant to the subset of data included in the indicated Test number.String
468AC50_Qualifier_TEST16'>','=', or '<'String
469AC50_uM_TEST16The concentration at which activity reaches 50% of the maximumFloatμM
470pAC50_M_TEST16Equal to -1*log10(AC50)String
471Hill_Slope_TEST16The slope at AC50Float
472S0_(%)_TEST16The fitted activity value at zero concentrationFloat%
473Sinf_(%)_TEST16The fitted activity value at infinite concentrationFloat%
474Num_Points_TEST16The number of data points used to generate the plotInteger
475Max_Activity_(%)_TEST16The maximum activity value observed, based on mean of replicates per concentrationFloat%
476Max_Activity_Conc_uM_TEST16The concentration at which the maximum activity is observedFloatμM
477Max_Concentration_uM_TEST16Maximum valid test concentrationFloatμM
478Activity_at_0uM_(%)_TEST16 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
479Activity_at_0.000001uM_(%)_TEST16 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
480Activity_at_0.000003uM_(%)_TEST16 (3e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
481Activity_at_0.000010uM_(%)_TEST16 (1e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
482Activity_at_0.000030uM_(%)_TEST16 (3e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
483Activity_at_0.000090uM_(%)_TEST16 (9e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
484Activity_at_0.000285uM_(%)_TEST16 (0.000285μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
485Activity_at_0.0008uM_(%)_TEST16 (0.0008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
486Activity_at_0.0026uM_(%)_TEST16 (0.0026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
487Activity_at_0.0075uM_(%)_TEST16 (0.0075μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
488Activity_at_0.0235uM_(%)_TEST16 (0.0235μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
489Activity_at_0.068uM_(%)_TEST16 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
490Activity_at_0.21uM_(%)_TEST16 (0.21μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
491Activity_at_0.6uM_(%)_TEST16 (0.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
492Activity_at_1.8uM_(%)_TEST16 (1.8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
493Activity_at_5.6uM_(%)_TEST16 (5.6μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
494Activity_at_16uM_(%)_TEST16 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%

* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: 1 R03 MH085673-01

Data Table (Concise)
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