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BioAssay: AID 651816

Cholera Quorum: HTS for inducers of light production in the absence of autoinducers using BH1578 (luxS deficient, cqsA deficient) Measured in Microorganism System Using Plate Reader - 2132-01_Agonist_Dose_DryPowder_Activity_Set4

Assay Overview: A modified strain of Vibrio cholerae used in this assay uses light production to signal quorum sensing. Vibrio cholerae is not naturally bioluminescent but the closely related species, Vibrio harveyi, produces light when the population is at a high density (i.e. a quorum is sensed). In the V. cholerae BH1578 strain, the V. harveyi luxCDABE (luciferase) operon was introduced as a more ..
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AID: 651816
Data Source: Broad Institute (2132-01_Agonist_Dose_DryPowder_Activity_Set4)
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Depositor Category: NIH Molecular Libraries Probe Production Network
BioAssay Version:
Deposit Date: 2012-11-27
Hold-until Date: 2013-08-01
Modify Date: 2013-08-02

Data Table ( Complete ):           View Active Data    View All Data
BioActive Compounds: 16
Related Experiments
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AIDNameTypeProbeComment
588521Broad Institute Quorum Sensing in Vibrio cholera: CqsS/LuxQ Agonist Probe ProjectSummary1 depositor-specified cross reference: Summary assay
588436Cholera Quorum: HTS for inducers of light production in the absence ofautoinducers using BH1578 (luxS deficient, cqsA deficient) Measured in Microorganism System Using Plate Reader - 2132-01_Agonist_SinglePoint_HTS_ActivityScreening same project related to Summary assay
602243Cholera Quorum: HTS for inducers of light production in the absence of autoinducers using BH1578 (luxS deficient, cqsA deficient) Measured in Microorganism System Using Plate Reader - 2132-01_Agonist_Dose_CherryPick_ActivityConfirmatory same project related to Summary assay
624140Cytotoxicity at 24 hours in dose with HeLa cells Measured in Cell-Based System Using Plate Reader - 2132-02_Agonist_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
624254Quorum LuxO: Assay for inducers of light production in the absence of#autoinducers using BH1651 (luxOD47E) Measured in Microorganism System Using Plate Reader - 2132-03_Agonist_Dose_CherryPick_ActivityConfirmatory same project related to Summary assay
624269Quorum LuxO: Assay for inducers of light production in the absence of#autoinducers using BH1651 (luxOD47E) Measured in Microorganism System Using Plate Reader - 2132-03_Agonist_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
624270Cholera Quorum: HTS for inducers of light production in the absence of#autoinducers using BH1578 (luxS deficient, cqsA deficient) Measured in Microorganism System Using Plate Reader - 2132-01_Agonist_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
624275Quorum CqsS/ LuxQ: Assay for inducers of light production in the absence of#autoinducers using WN1103 (delta luxQ) Measured in Microorganism System Using Plate Reader - 2132-05_Agonist_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
624281Quorum CqsS/ LuxQ: Assay for inducers of light production in the absence#of autoinducers using DH231 (delta cqsS) Measured in Microorganism System Using Plate Reader - 2132-04_Agonist_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651774Cytotoxicity at 24 hours in dose with HeLa cells Measured in Cell-Based System Using Plate Reader - 2132-02_Agonist_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
651808Quorum CqsS/ LuxQ: Assay for inducers of light production in the absence of autoinducers using DH231 (delta cqsS) Measured in Microorganism System Using Plate Reader - 2132-04_Agonist_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
651809Cholera Quorum: HTS for inducers of light production in the absence of autoinducers using BH1578 (luxS deficient, cqsA deficient) Measured in Microorganism System Using Plate Reader - 2132-01_Agonist_Dose_DryPowder_Activity_Set3Confirmatory same project related to Summary assay
651841Quorum CqsS/ LuxQ: Assay for inducers of light production in the absence of autoinducers using WN1103 (delta luxQ) Measured in Microorganism System Using Plate Reader - 2132-05_Agonist_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
651847Quorum LuxO: Assay for inducers of light production in the absence of autoinducers using BH1651 (luxOD47E) Measured in Microorganism System Using Plate Reader - 2132-03_Agonist_Dose_DryPowder_Activity_Set3Confirmatory same project related to Summary assay
651854Cholera Quorum: HTS for inducers of light production in the absence of autoinducers using BH1578 (luxS deficient, cqsA deficient) Measured in Microorganism System Using Plate Reader - 2132-01_Agonist_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
651864Cytotoxicity at 24 hours in dose with HeLa cells Measured in Cell-Based System Using Plate Reader - 2132-02_Agonist_Dose_DryPowder_Activity_Set3Confirmatory same project related to Summary assay
651905CqsS In vitro Kinase Assay Measured in Biochemical System Using Imaging - 2132-10_Agonist_Dose_DryPowder_ActivityOther same project related to Summary assay
652219Quorum LuxO: Assay for change of GFP production in the absence of autoinducers using SL353 (luxOD47E, qrr4-gp) Measured in Microorganism System Using Plate Reader - 2132-06_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
652239Cholera Quorum: HTS for inducers of light production in the absence of autoinducers using BH1578 (luxS deficient, cqsA deficient) Measured in Microorganism System Using Plate Reader - 2132-01_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
686928Cytotoxicity at 24 hours in dose with HeLa cells Measured in Cell-Based System Using Plate Reader - 2132-02_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
686929Cholera Quorum: HTS for inducers of light production in the absence of autoinducers using BH1578 (luxS deficient, cqsA deficient) Measured in Microorganism System Using Plate Reader - 2132-01_Inhibitor_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
686930Quorum CqsS/ LuxQ: Assay for inducers of light production in the absence of autoinducers using WN1103 (delta luxQ) Measured in Microorganism System Using Plate Reader - 2132-05_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
686931Quorum CqsS/ LuxQ: Assay for inducers of light production in the absence of autoinducers using DH231 (delta cqsS) Measured in Microorganism System Using Plate Reader - 2132-04_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
686936In vitro LuxO ATPase Assay Measured in Biochemical System Using Plate Reader - 2132-11_Inhibitor_Dose_DryPowder_ActivityOther same project related to Summary assay
686939Counterscreen for activators of the function of SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2, BRM): Luminescence-based cell-based high throughput screening assay to identify non-selective compounds using the VP16 reporter assayScreening same project related to Summary assay
686942Quorum LuxO: Assay for change of GFP production in the absence of autoinducers using SL353 (luxOD47E, qrr4-gp) Measured in Microorganism System Using Plate Reader - 2132-06_Inhibitor_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
686945Broad Institute Quorum Sensing in Vibrio cholera: LuxO Inhibitor Probe ProjectSummary same project related to Summary assay
Description:
Keywords: V. cholerae, cqsA, luxS mutant, quorum sensing, luminescence
Assay Overview: A modified strain of Vibrio cholerae used in this assay uses light production to signal quorum sensing. Vibrio cholerae is not naturally bioluminescent but the closely related species, Vibrio harveyi, produces light when the population is at a high density (i.e. a quorum is sensed). In the V. cholerae BH1578 strain, the V. harveyi luxCDABE (luciferase) operon was introduced as a cosmid and the operon is activated by endogenous mechanisms to elicit light upon reaching a quorum. In addition, the BH1578 strain is a cqsA, luxS double mutant that lacks both autoinducer synthases (CAI-1 and AI-2). BH1578 does not generate light in the absence of exogenous autoinducers but bioluminescence can be stimulated up to 10,000-fold by adding 1 uM (saturating) CAI-1. Bacteria are plated into 384 well plates and compound is added by the pin transfer method. CAI-1 will be used as the positive control. In addition to luminescence, the confluency of each well is measured at an absorbance of 600 nM.

Expected Outcome: Molecules that induce light production in BH1578 in the absence of exogenous autoinducers will cause an increased signal and register as hits in this assay. As a reminder of the rationale for the screen, the accumulation of autoinducers normally represses virulence factor expression and biofilm formation in V. cholerae.
Protocol
Vibrio cholerae quorum-sensing modulator bioassay
Reporter strain:
1.BH1578 (V. cholerae del cqsA del luxQ carrying the pBB1 cosmid, which contains the V. harveyi luxCDABE luciferase operon)
Materials:
LB Medium: Dissolve in 10 g/L Tryptone, 5 g/L Yeast Extract, and 10 g/L NaCl in distilled water, autoclave, store at room temperature
Tetracycline (10 mg/mL): Dissolve 10 mg tetracycline in 1 mL 100% ethanol, store at -20C, protect from light
LB/tet: add 1 mL tetracycline (10 mg/mL) to 1L of LB medium. Final concentration of tetracycline is 10 microg/mL. Prepare it fresh on a daily basis.
CAI-1 stock: Dissolve CAI-1 in DMSO to 50 mM (10.7 mg/mL), store at -20C
Procedures:
1.Grow up the BH1578 reporter strain in 100 mL LB/Tet at 30C for >16 hours with shaking (200 rpm). The final OD600 of each culture should be > 3.0
2.Dilute the culture to an OD600 of 0.9 with LB/Tet, mix well. (Note: avoid biofilm aggregates in the culture, a low speed centrifugation (200 rpm for 1 min) should remove most aggregates)
3.Add 20 uL of LB-Tet per 384 well assay plate with the Thermo Combi fluid dispenser. Add 150 nL of compound per well.
4.Dispense 10 microL of diluted culture into each well of a 384 well plate (Black with clear bottom Greiner 781096 plates). Compounds are screened at 20 microM final concentration. 1 uM CAI-1 was used as the positive control.
5.Incubate the plates at 30 degrees C for 6 hours.
5.Measure bioluminescence (USLum(384)) and OD600 in a Perkin-Elmer Envison Multilabel Reader
Comment
PRESENCE OF CONTROLS: Neutral control wells (NC; n=132) and positive control wells (PC; n=18) were included on every plate.
EXPECTED OUTCOME: Active compounds result in increasing readout signal.
ACTIVE CONCENTRATION LIMIT:
For each sample, the highest valid tested concentration (Max_Concentration) was determined and the active concentration limit (AC_limit) was set to equal Max_Concentration.
NORMALIZATION:
The raw signals of the plate wells were normalized using the 'Stimulators Minus Neutral Controls' method in Genedata Assay Analyzer (v7.0.3):
The median raw signal of the intraplate neutral control wells was set to a normalized activity value of 0.
The median raw signal of the intraplate positive control wells was set to a normalized activity value of 100.
Experimental wells values were scaled to this range.
PATTERN CORRECTION: No plate pattern correction algorithm from Genedata Condoseo (v.7.0.3) was applied.
MEASUREMENT USED TO DETERMINE ACTIVE CONCENTRATION (AC): absACnn, the concentration at which the curve crosses threshold 40.0
AC values were calculated using the curve fitting strategies in Genedata Screener Condoseo (7.0.3).
AC values were calculated up to the active concentration limit described for each sample.
pAC was set to equal -1*log10(AC)
PUBCHEM_ACTIVITY_OUTCOME:
Activity_Outcome = 1 (inactive) when:
a) compound shows activity but in a direction opposite to the expected outcome
in these cases, values describing curve fitting parameters (Sinf, S0, Hill Slope, log_AC50, log_AC50_SE) are set to null
b) curve fit is constant where activity is > -30% and < 30% at all tested concentrations, or
c) AC > AC_limit
Activity_Outcome = 2 (active) when:
AC <= AC_limit
Activity_Outcome = 3 (inconclusive) when:
a) Curve fitting strategy resulted in a constant fit with activity >= 30% but <= 70%, or
b) The fit was deemed not valid due to poor fit quality.
PUBCHEM_ACTIVITY_SCORE:
If PUBCHEM_ACTIVITY_OUTCOME = 1 (inactive) or 3 (inconclusive),
then PUBCHEM_ACTIVITY_SCORE = 0
If PUBCHEM_ACTIVITY_OUTCOME = 2 (active)
then PUBCHEM_ACTIVITY_SCORE = (10)(pAC)
Scores relate to AC in this manner:
120 = 1 pM
90 = 1 nM
60 = 1 uM
30 = 1 mM
0 = 1 M
When the active concentration (AC) is calculated to be greater than the highest valid tested concentration (Max_Concentration), the PUBCHEM_ACTIVITY_SCORE is calculated using Max_Concentration as the basis.
When the active concentration (AC) is calculated to be less than the lowest tested concentration, the PUBCHEM_ACTIVITY_SCORE is calculated using the lowest tested concentration as the basis.
Note:
The individual dose data point columns ('Activity_at_xxuM') reported here represent the median of valid (unmasked) replicate observations at each concentration. These values are the inputs to a curve fitting algorithm.
All other data columns represent values which are derived during the curve fitting algorithm; this may sometimes include automatic further masking of some replicate data points.
Occasionally this results in perceived inconsistencies: for example, between the derived 'Maximal_Activity' and the apparent most active data point.
Categorized Comment - additional comments and annotations
From PubChem:
Assay Format: Organism-based
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1AbsAC40_Qualifier<, =, or >String
2AbsAC40_uM*The concentration at which the fitted curve passes activity threshold 40.FloatμM
3pAbsAC40_MEqual to -1*log10(AbsAC40).Float
4ACTIVITY_OUTCOME_TEST1The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
5ACTIVITY_SCORE_TEST1The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
6ASSAYDATA_COMMENT_TEST1Comments relevant to the subset of data included in the indicated Test number.String
7AbsAC40_Qualifier_TEST1<, =, or >String
8AbsAC40_uM_TEST1The concentration at which the fitted curve passes activity threshold nn.FloatμM
9pAbsAC40_M_TEST1Equal to -1*log10(AbsACnn).Float
10Hill_Slope_TEST1The slope at AC50Float
11S0_(%)_TEST1The fitted activity value at zero concentrationFloat%
12Sinf_(%)_TEST1The fitted activity value at infinite concentrationFloat%
13Num_Points_TEST1The number of data points used to generate the plotInteger
14Max_Activity_(%)_TEST1The maximum activity value observed, based on mean of replicates per concentrationFloat%
15Max_Activity_Conc_uM_TEST1The concentration at which the maximum activity is observedFloatμM
16Max_Concentration_uM_TEST1Maximum valid test concentrationFloatμM
17Activity_at_0uM_(%)_TEST1 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
18Activity_at_0.000001uM_(%)_TEST1 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
19Activity_at_0.000002uM_(%)_TEST1 (2e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
20Activity_at_0.000006uM_(%)_TEST1 (6e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
21Activity_at_0.000018uM_(%)_TEST1 (1.8e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
22Activity_at_0.000050uM_(%)_TEST1 (5e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
23Activity_at_0.00016uM_(%)_TEST1 (0.00016μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
24Activity_at_0.00046uM_(%)_TEST1 (0.00046μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
25Activity_at_0.00135uM_(%)_TEST1 (0.00135μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
26Activity_at_0.0042uM_(%)_TEST1 (0.0042μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
27Activity_at_0.012uM_(%)_TEST1 (0.012μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
28Activity_at_0.038uM_(%)_TEST1 (0.038μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
29Activity_at_0.11uM_(%)_TEST1 (0.11μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
30Activity_at_0.35uM_(%)_TEST1 (0.35μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
31Activity_at_1uM_(%)_TEST1 (1μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
32Activity_at_3uM_(%)_TEST1 (3μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
33Activity_at_9uM_(%)_TEST1 (9μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
34Activity_at_28.5uM_(%)_TEST1 (28.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
35Activity_at_80uM_(%)_TEST1 (80μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
36ACTIVITY_OUTCOME_TEST2The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
37ACTIVITY_SCORE_TEST2The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
38ASSAYDATA_COMMENT_TEST2Comments relevant to the subset of data included in the indicated Test number.String
39AbsAC40_Qualifier_TEST2<, =, or >String
40AbsAC40_uM_TEST2The concentration at which the fitted curve passes activity threshold nn.FloatμM
41pAbsAC40_M_TEST2Equal to -1*log10(AbsACnn).Float
42Hill_Slope_TEST2The slope at AC50Float
43S0_(%)_TEST2The fitted activity value at zero concentrationFloat%
44Sinf_(%)_TEST2The fitted activity value at infinite concentrationFloat%
45Num_Points_TEST2The number of data points used to generate the plotInteger
46Max_Activity_(%)_TEST2The maximum activity value observed, based on mean of replicates per concentrationFloat%
47Max_Activity_Conc_uM_TEST2The concentration at which the maximum activity is observedFloatμM
48Max_Concentration_uM_TEST2Maximum valid test concentrationFloatμM
49Activity_at_0uM_(%)_TEST2 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
50Activity_at_0.000001uM_(%)_TEST2 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
51Activity_at_0.000002uM_(%)_TEST2 (2e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
52Activity_at_0.000006uM_(%)_TEST2 (6e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
53Activity_at_0.000018uM_(%)_TEST2 (1.8e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
54Activity_at_0.000050uM_(%)_TEST2 (5e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
55Activity_at_0.00016uM_(%)_TEST2 (0.00016μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
56Activity_at_0.00046uM_(%)_TEST2 (0.00046μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
57Activity_at_0.00135uM_(%)_TEST2 (0.00135μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
58Activity_at_0.0042uM_(%)_TEST2 (0.0042μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
59Activity_at_0.012uM_(%)_TEST2 (0.012μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
60Activity_at_0.038uM_(%)_TEST2 (0.038μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
61Activity_at_0.11uM_(%)_TEST2 (0.11μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
62Activity_at_0.35uM_(%)_TEST2 (0.35μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
63Activity_at_1uM_(%)_TEST2 (1μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
64Activity_at_3uM_(%)_TEST2 (3μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
65Activity_at_9uM_(%)_TEST2 (9μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
66Activity_at_28.5uM_(%)_TEST2 (28.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
67Activity_at_80uM_(%)_TEST2 (80μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
68ACTIVITY_OUTCOME_TEST3The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
69ACTIVITY_SCORE_TEST3The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
70ASSAYDATA_COMMENT_TEST3Comments relevant to the subset of data included in the indicated Test number.String
71AbsAC40_Qualifier_TEST3<, =, or >String
72AbsAC40_uM_TEST3The concentration at which the fitted curve passes activity threshold nn.FloatμM
73pAbsAC40_M_TEST3Equal to -1*log10(AbsACnn).Float
74Hill_Slope_TEST3The slope at AC50Float
75S0_(%)_TEST3The fitted activity value at zero concentrationFloat%
76Sinf_(%)_TEST3The fitted activity value at infinite concentrationFloat%
77Num_Points_TEST3The number of data points used to generate the plotInteger
78Max_Activity_(%)_TEST3The maximum activity value observed, based on mean of replicates per concentrationFloat%
79Max_Activity_Conc_uM_TEST3The concentration at which the maximum activity is observedFloatμM
80Max_Concentration_uM_TEST3Maximum valid test concentrationFloatμM
81Activity_at_0uM_(%)_TEST3 (0μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
82Activity_at_0.000001uM_(%)_TEST3 (1e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
83Activity_at_0.000002uM_(%)_TEST3 (2e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
84Activity_at_0.000006uM_(%)_TEST3 (6e-06μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
85Activity_at_0.000018uM_(%)_TEST3 (1.8e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
86Activity_at_0.000050uM_(%)_TEST3 (5e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
87Activity_at_0.00016uM_(%)_TEST3 (0.00016μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
88Activity_at_0.00046uM_(%)_TEST3 (0.00046μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
89Activity_at_0.00135uM_(%)_TEST3 (0.00135μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
90Activity_at_0.0042uM_(%)_TEST3 (0.0042μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
91Activity_at_0.012uM_(%)_TEST3 (0.012μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
92Activity_at_0.038uM_(%)_TEST3 (0.038μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
93Activity_at_0.11uM_(%)_TEST3 (0.11μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
94Activity_at_0.35uM_(%)_TEST3 (0.35μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
95Activity_at_1uM_(%)_TEST3 (1μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
96Activity_at_3uM_(%)_TEST3 (3μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
97Activity_at_9uM_(%)_TEST3 (9μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
98Activity_at_28.5uM_(%)_TEST3 (28.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
99Activity_at_80uM_(%)_TEST3 (80μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%

* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: R03 MH094166-01

Data Table (Concise)
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