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BioAssay: AID 651592

SAR analysis of molecule cystic fibrosis induced NFkb Inhibitors in a panel assay

The inflammatory response in Cystic Fibrosis (CF) is a complex interplay between several factors. It has been suggested that pro-inflammatory cytokines are elevated in the epithelial lining fluid samples of CF patients(1). Conversely, expressions of anti-inflammatory cytokines are reduced(2). 90% of all CF deaths occurs from defective lung function. The activation of NFkB via toll-like receptors following bacterial infection is principally involved in the regulation of lung inflammation in CF(3,4) ..more
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 Tested Compounds
 Tested Compounds
All(35)
 
 
Active(5)
 
 
Inactive(30)
 
 
 Tested Substances
 Tested Substances
All(35)
 
 
Active(5)
 
 
Inactive(30)
 
 
AID: 651592
Data Source: Burnham Center for Chemical Genomics (SBCCG-A905-CF-PAF-Panel-Assay)
BioAssay Type: Panel, Confirmatory, Concentration-Response Relationship Observed
Depositor Category: NIH Molecular Libraries Probe Production Network
BioAssay Version:
Deposit Date: 2012-09-20
Hold-until Date: 2013-03-03
Modify Date: 2013-03-03

Data Table ( Complete ):           Active    All
Target
BioActive Compounds: 5
Depositor Specified Assays
AIDNameTypeComment
588850uHTS identification of cystic fibrosis induced NFkb Inhibitors in a fluoresence assayscreening
588858Summary assay for small molecule cystic fibrosis induced NFkb Inhibitorssummary
Description:
Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG)
Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego CA)
Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN)
Grant Number: IR21 NS061743-01
Assay Provider: Rangan Maitra, Ph.D., RTI International University

The inflammatory response in Cystic Fibrosis (CF) is a complex interplay between several factors. It has been suggested that pro-inflammatory cytokines are elevated in the epithelial lining fluid samples of CF patients(1). Conversely, expressions of anti-inflammatory cytokines are reduced(2). 90% of all CF deaths occurs from defective lung function. The activation of NFkB via toll-like receptors following bacterial infection is principally involved in the regulation of lung inflammation in CF(3,4)

This project proposes to develop and optimize a Pseudomonas aeruginosa filtrate (PAF)-induced NFkB-GFP reporter assay using immortalized CF airway epithelial cell line (KKLEB) and screen the MLPCN library for inhibitors of PAF-induced NFkB pathway and distinguish compounds that are inhibiting via the toll-like receptor NFkB pathway to treat lung inflammation in CF.

These dose response assays were developed and performed as a follow-up to "Single concentration confirmation of uHTS hits for cystic fibrosis induced NFkb Inhibitors in a fluorescence assay multiplexed with luminescence cytotoxicity assay." AID 588850 and to study the structure-activity relationship on analogs of the confirmed hits. Compounds are either acquired from commercial sources or synthesized internally.. Compounds active in the CF-PAF and PAF induced IL8 assays and inactive in the TNFa induced IL8 assay are desired.

REFERENCES
1. Bonfield, T.L., Konstan, M. W. & Berger, M. Altered respiratory epithelial cell cytokine production in cystic fibrosis. J Allergy Clin Immunol 104, 72-8 (1999).
2. Bonfield, T.L et al. Normal bronchial epithelial cells constitutively produce the anti-inflammatory cytokine interleukin-10, which is downregulated in cystic fibrosis. Am J Respir Cell Mol Biol 13, 257-61 (1995).
3. Zhang, Z., Louboutin, J.P., Weiner, D.J., Goldberg, J.B. & Wilson, J. M. Human airway epithelial cells sense Pseudomonas aeruginosa infection via recognition of flagellin by toll-like receptor 5. Infect Immunol 73, 7151-60 (2005).
4. Greene, C. M. et al. TLR-induced inflammation in cystic fibrosis and non-cystic fibrosis airway epithelial cells. J. Immunol 174, 1638-46 (2005).
Panel Information
CF-PAF panel drypowder
    Data Table(Active)    Data Table(All)Show more
PID§NameSubstancePanel TargetsDescription
ActiveInactive
1IC50_CF-PAF_Mean629cystic fibrosis transmembrane conductance regulator [Homo sapiens] [gi:90421313]
Mean IC50 value
2IC50_1_CF-PAFcystic fibrosis transmembrane conductance regulator [Homo sapiens] [gi:90421313]
IC50 value determined using a sigmoidal dose response equation
3IC50_2_CF-PAFcystic fibrosis transmembrane conductance regulator [Homo sapiens] [gi:90421313]
IC50 value determined using a sigmoidal dose response equation
4IC50_3_CF-PAFcystic fibrosis transmembrane conductance regulator [Homo sapiens] [gi:90421313]
IC50 value determined using a sigmoidal dose response equation
5IC50_4_CF-PAFcystic fibrosis transmembrane conductance regulator [Homo sapiens] [gi:90421313]
IC50 value determined using a sigmoidal dose response equation
6IC50_5_CF-PAFcystic fibrosis transmembrane conductance regulator [Homo sapiens] [gi:90421313]
IC50 value determined using a sigmoidal dose response equation
7IC50_6_CF-PAFcystic fibrosis transmembrane conductance regulator [Homo sapiens] [gi:90421313]
IC50 value determined using a sigmoidal dose response equation
8IC50_PAF-IL8_Mean341interleukin-8 precursor [Homo sapiens] [gi:10834978]
Mean IC50 value
9IC50_1_PAF-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
10IC50_2_PAF-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
11IC50_3_PAF-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
12IC50_4_PAF-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
13IC50_5_PAF-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
14IC50_6_PAF-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
15IC50_TNFa-IL8_Mean287interleukin-8 precursor [Homo sapiens] [gi:10834978]
Mean IC50 value
16IC50_1_TNFa-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
17IC50_2_TNFa-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
18IC50_3_TNFa-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
19IC50_4_TNFa-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
20IC50_5_TNFa-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation
21IC50_6_TNFa-IL8interleukin-8 precursor [Homo sapiens] [gi:10834978]
IC50 value determined using a sigmoidal dose response equation

§ Panel component ID.
Protocol
CF-PAF assay
Assay Materials:
KKLEB-NFkB-GFP cells (Assay Provider)
Pseudomonas aeruginosa filtrate (PAF) (Assay Provider)
F12 (Invitrogen 11765)
Fetal Bovine Serum (Hyclone SH30396.03)
Penicillin Streptomycin solution (Invitrogen 15140122)
L-glutamine (100X) (Invitrogen 25030081)
TrypLE (Invitrogen 12563)
DPBS without calcium and magnesium (1X) (Hyclone SH30028.02)
Corning tissue culture flasks
Black CellBind 1536-well plates (Corning 3833)

I. Compound Addition and Cell Plating:
1- Transfer test compounds to columns 5-44 and DMSO to columns 1-4 and 45-48 using the Labcyte ECHO 555. Transfer volume of test compound is 40 nL, 20 nL, 10 nL, 5 nL, and 2.5 nL from 10mM stock and 40 nL, 20 nL, 10 nL, 5 nL, and 2.5 nL from 0.3125 mM stock for dose response which DMSO backfill to total volume of 40 nL per well. Compound concentration range from 79 uM to 0.15 uM (1:2 dilution, 10 concentration). DMSO transfer volume is 40 nL, final DMSO concentration is 1%.
2- Dispense 3 uL/well of cells at 5X10e5 cells/mL to the whole plate (plate cells in 2% FBS assay media).
3- Spin down plates on Eppendorf centrifuge 5810 at 500 rpm for 1 minute.
4- Put Kalypsys metal lids on plates, incubate plates at 37 degrees C with 5% CO2 for 2 hours.

II. Reagent Addition:
5- Dispense 3 uL/well of 13.33% LB (dilute in serum free assay media) to columns 1-4 final LB concentration is 5%.
6- Dispense 3 uL/well of 13.33% PAF (dilute in serum free assay media) to columns 5-48, final PAF concentration is 5%.
7- Spin down plates Eppendorf centrifuge 5810 at 1000 rpm for 1 minute.
8- Put Kalypsys metal lids on plates, and incubate plates at 37 degrees C with 5% CO2 overnight.

III. Reading plates:
9- Spin plates upside down with a container at 1000 rpm for 15 sec. Dab them with a tissue to dry them and Read immediately on Envision for GFP fluorescence.

PAF induced IL8
Assay Materials:
KKLEB-NFkB-GFP cells (Assay Provider)
Pseudomonas aeruginosa filtrate (PAF) (Assay Provider)
F12 (Invitrogen 11765)
Fetal Bovine Serum (Hyclone SH30396.03)
Penicillin Streptomycin solution (Invitrogen 15140122)
L-glutamine (100X) (Invitrogen 25030081)
TrypLE (Invitrogen 12563)
DPBS without calcium and magnesium (1X) (Hyclone SH30028.02)
Corning tissue culture flasks
Black CellBind 1536-well plates (Corning 3833)
Human IL8 Kit (20,000 tests) (Cisbio 62IL8PEC)

I. Compound Addition and Cell Plating:
1- Transfer test compounds to columns 5-44 and DMSO to columns 1-4 and 45-48 using the Labcyte ECHO 555. Transfer volume of test compound is 40 nL, 20 nL, 10 nL, 5 nL, and 2.5 nL from 10 mM stock and 40 nL, 20 nL, 10 nL, 5 nL, and 2.5 nL from 0.3125 mM stock for dose response which DMSO backfill to total volume of 40 nL per well. Compound concentration range from 79 uM to 0.15 uM (1:2 dilution, 10 concentration). DMSO transfer volume is 40 nL, final DMSO concentration is 1%.
2- Dispense 3 uL/well of cells at 8.3X10e4 cells/mL to the whole plate (plate cells in 2% FBS assay media).
3- Spin down plates on Eppendorf centrifuge 5810 at 500 rpm for 1 minute.
4- Put Kalypsys metal lids on plates, incubate plates at 37 degrees C with 5% CO2 for 2 hours.

II. Reagent Addition:
5- Prepare antibodies according to Cisbio protocol and add LB or PAF making it 1.33% working solution.
6- Dispense 2 uL/well of antibodies mix with 1.33% LB to columns 1-4, final LB concentration is 0.5%.
7- Dispense 2 uL/well of antibodies mix with 1.33% PAF to columns 5-48, final PAF concentration is 0.5%.
8- Spin down plates Eppendorf centrifuge 5810 at 1000 rpm for 1 minute.
9- Put Kalypsys metal lids on plates, and incubate plates at 37 degrees C with 5% CO2 overnight.

III. Reading plates:
10- Add 5 ul reconstitution buffer to all wells.
11- Spin down plates Eppendorf centrifuge 5810 at 2000 rpm for 2 minutes.
12- Read immediately on envision for FRET.


TNFa induced IL8
Assay Materials:
KKLEB-NFkB-GFP cells (Assay Provider)
TNFa Recombinant Human Protein (Biomol 908-066)
F12 (Invitrogen 11765)
Fetal Bovine Serum (Hyclone SH30396.03)
Penicillin Streptomycin solution (Invitrogen 15140122)
L-glutamine (100X) (Invitrogen 25030081)
TrypLE (Invitrogen 12563)
DPBS without calcium and magnesium (1X) (Hyclone SH30028.02)
Corning tissue culture flasks
Black CellBind 1536-well plates (Corning 3833)
Human IL8 Kit (20,000 tests) (Cisbio 62IL8PEC)

I. Compound Addition and Cell Plating:
1- Transfer test compounds to columns 5-44 and DMSO to columns 1-4 and 45-48 using the Labcyte ECHO 555. Transfer volume of test compound is 40 nL, 20 nL, 10 nL, 5 nL, and 2.5 nL from 10 mM stock and 40 nL, 20 nL, 10 nL, 5 nL, and 2.5 nL from 0.3125 mM stock for dose response which DMSO backfill to total volume of 40 nL per well. Compound concentration range from 79 uM to 0.15 uM (1:2 dilution, 10 concentration). DMSO transfer volume is 40 nL, final DMSO concentration is 1%.
2- Dispense 3 uL/well of cells at 8.3X10e4 cells/mL to the whole plate (plate cells in 2% FBS assay media).
3- Spin down plates on Eppendorf centrifuge 5810 at 500 rpm for 1 minute.
4- Put Kalypsys metal lids on plates, incubate plates at 37 degrees C with 5% CO2 for 2 hours.

II. Reagent Addition:
5- Prepare antibodies according to Cisbio protocol and add media or TNFa making it working solution.
6- Dispense 2 uL/well of antibodies mix with media to columns 1-4
7- Dispense 2 uL/well of antibodies mix with TNFa to columns 5-48, final TNFa concentration is 10ng/ml.
8- Spin down plates Eppendorf centrifuge 5810 at 1000 rpm for 1 minute.
9- Put Kalypsys metal lids on plates, and incubate plates at 37 degrees C with 5% CO2 overnight.

III. Reading plates:
10- Add 5 ul reconstitution buffer to all wells.
11- Spin down plates Eppendorf centrifuge 5810 at 2000 rpm for 2 minutes.
12- Read immediately on envision for FRET.
Comment
Compounds that are found to be active the the CF-PAF and PAF induced IL8 assays but inactive in the TNFa induced IL8 assay are marked as active for the panel and given the score of the CF-PAF assay.


Compounds with IC50_CF-PAF_mean0 <= 10 uM are marked as active in the CF-PAF assay

Compounds with IC50_PAF_IL8_mean IC50 <= 5* IC50_CF-PAF_mean are marked as active in the PAF induced IL8 assay

Compounds with IC50_TNFa_IL8 <= 5*IC50_CF-PAF_mean are marked as active in the TNFa induced IL8 assay

To simplify the distinction between the inactives of the primary screen and of the confirmatory screening stage, the Tiered Activity Scoring System was developed and implemented. Its utilization for the assay is described below.

Activity Scoring
Activity scoring rules were devised to take into consideration compound efficacy, its potential interference with the assay and the screening stage that the data was obtained. Details of the Scoring System will be published elsewhere. Briefly, the outline of the scoring system utilized for the assay is as follows:

1) First tier (0-40 range) is reserved for primary and single-concentration confirmation screening data and is not applicable in this assay

2) Second tier (41-80 range) is reserved for dose-response confirmation data and is not applicable in this assay

3) Third tier (81-100 range) is reserved for resynthesized true positives and their analogues
a. Inactive compounds of the confirmatory stage are assigned a score value equal 81.
b. The score is linearly correlated with a compound potency and, in addition, provides a measure of the likelihood that the compound is not an artifact based on the available information.
c. The Hill coefficient is taken as a measure of compound behavior in the assay via an additional scaling factor QC:

QC = 2.6*[exp(-0.5*nH^2) - exp(-1.5*nH^2)]

This empirical factor prorates the likelihood of target-specific compound effect vs. its non-specific behavior in the assay. This factor is based on expectation that a compound with a single mode of action that achieved equilibrium in this assay demonstrates the Hill coefficient value of 1. Compounds deviating from that behavior are penalized proportionally to the degree of their deviation.
d. Summary equation that takes into account the items discussed above is

Score = 82 + 3*(pIC50 - 3)*QC,

where pIC50 is a negative log(10) of the IC50 value expressed in mole/L concentration units. This equation results in the Score values above 85 for compounds that demonstrate high potency and predictable behavior. Compounds that are inactive in the assay or whose concentration-dependent behavior are likely to be an artifact of that assay will generally have lower Score values.
Result Definitions
Show more
TIDNameDescriptionPID§Panel TargetsHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1Outcome_CF-PAF1cystic fibrosis transmembrane conductance regulator [Homo sapiens]Outcome
2PUBCHEM_ACTIVITY_SCORE_CF-PAF1Integer
3IC50_CF-PAF_Mean_QualifierThis qualifier is to be used with the next TID, IC50_Mean. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
4IC50_CF-PAF_Mean*IC50 value determined using a sigmoidal dose response equation1cystic fibrosis transmembrane conductance regulator [Homo sapiens]FloatμM
5Outcome_PAF-IL88interleukin-8 precursor [Homo sapiens]Outcome
6PUBCHEM_ACTIVITY_SCORE_PAF-IL8Integer
7IC50_PAF-IL8_Mean_QualifierThis qualifier is to be used with the next TID, IC50_Mean. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that value8interleukin-8 precursor [Homo sapiens]String
8IC50_PAF-IL8_Mean*IC50 value determined using a sigmoidal dose response equation8FloatμM
9Outcome_TNFa-IL815interleukin-8 precursor [Homo sapiens]Outcome
10PUBCHEM_ACTIVITY_SCORE_TNFa-IL815Integer
11IC50_TNFa-IL8_Mean_QualifierThis qualifier is to be used with the next TID, IC50_TNFa-IL8_Mean. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
12IC50_TNFa-IL8_Mean*IC50 value determined using a sigmoidal dose response equation15interleukin-8 precursor [Homo sapiens]FloatμM
13IC50_Qualifier_1_CF-PAFThis qualifier is to be used with the next TID, IC50_1_CF-PAF. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that value2cystic fibrosis transmembrane conductance regulator [Homo sapiens]String
14IC50_1_CF-PAF*IC50 value determined using a sigmoidal dose response equation2FloatμM
15Std.Err(IC50)_1_CF-PAFStandard Error of the IC50 valueFloatμM
16nH_1_CF-PAFHill coefficient determined using sigmoidal dose response equationFloat
17Excluded_Points_first_point_CF-PAFFlags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
18% Activity at 66 uM_first_point_CF-PAF (66μM**)% Activity at the test concentrationFloat%
19% Activity at 33 uM_first_point_CF-PAF (33μM**)% Activity at the test concentrationFloat%
20% Activity at 16.5 uM_first_point_CF-PAF (16.5μM**)% Activity at the test concentrationFloat%
21% Activity at 8.25 uM_first_point_CF-PAF (8.25μM**)% Activity at the test concentrationFloat%
22% Activity at 4.125 uM_first_point_CF-PAF (4.125μM**)% Activity at the test concentrationFloat%
23% Activity at 2.0625 uM_first_point_CF-PAF (2.0625μM**)% Activity at the test concentrationFloat%
24% Activity at 1.03125 uM_first_point_CF-PAF (1.03125μM**)% Activity at the test concentrationFloat%
25% Activity at 0.515625 uM_first_point_CF-PAF (0.515625μM**)% Activity at the test concentrationFloat%
26% Activity at 0.2578125 uM_first_point_CF-PAF (0.257812μM**)% Activity at the test concentrationFloat%
27% Activity at 0.1289062 uM_first_point_CF-PAF (0.128906μM**)% Activity at the test concentrationFloat%
28IC50_Qualifier_2_CF-PAFThis qualifier is to be used with the next TID, IC50_2_CF-PAF. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
29IC50_2_CF-PAF*IC50 value determined using a sigmoidal dose response equation3cystic fibrosis transmembrane conductance regulator [Homo sapiens]FloatμM
30Std.Err(IC50)_2_CF-PAFStandard Error of the IC50 valueFloatμM
31nH_2_CF-PAFHill coefficient determined using sigmoidal dose response equationFloat
32Excluded_Points_second_point_CF-PAFFlags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
33% Activity at 66 uM_second_point_CF-PAF (66μM**)% Activity at the test concentrationFloat%
34% Activity at 33 uM_second_point_CF-PAF (33μM**)% Activity at the test concentrationFloat%
35% Activity at 16.5 uM_second_point_CF-PAF (16.5μM**)% Activity at the test concentrationFloat%
36% Activity at 8.25 uM_second_point_CF-PAF (8.25μM**)% Activity at the test concentrationFloat%
37% Activity at 4.125 uM_second_point_CF-PAF (4.125μM**)% Activity at the test concentrationFloat%
38% Activity at 2.0625 uM_second_point_CF-PAF (2.0625μM**)% Activity at the test concentrationFloat%
39% Activity at 1.03125 uM_second_point_CF-PAF (1.03125μM**)% Activity at the test concentrationFloat%
40% Activity at 0.515625 uM_second_point_CF-PAF (0.515625μM**)% Activity at the test concentrationFloat%
41% Activity at 0.2578125 uM_second_point_CF-PAF (0.257812μM**)% Activity at the test concentrationFloat%
42% Activity at 0.1289062 uM_second_point_CF-PAF (0.128906μM**)% Activity at the test concentrationFloat%
43IC50_Qualifier_3_CF-PAFThis qualifier is to be used with the next TID, IC50_3_CF-PAF. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
44IC50_3_CF-PAF*IC50 value determined using a sigmoidal dose response equation4cystic fibrosis transmembrane conductance regulator [Homo sapiens]FloatμM
45Std.Err(IC50)_3_CF-PAFStandard Error of the IC50 valueFloatμM
46nH_3_CF-PAFHill coefficient determined using sigmoidal dose response equationFloat
47Excluded_Points_third_point_CF-PAFFlags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
48% Activity at 66 uM_third_point_CF-PAF (66μM**)% Activity at the test concentrationFloat%
49% Activity at 33 uM_third_point_CF-PAF (33μM**)% Activity at the test concentrationFloat%
50% Activity at 16.5 uM_third_point_CF-PAF (16.5μM**)% Activity at the test concentrationFloat%
51% Activity at 8.25 uM_third_point_CF-PAF (8.25μM**)% Activity at the test concentrationFloat%
52% Activity at 4.125 uM_third_point_CF-PAF (4.125μM**)% Activity at the test concentrationFloat%
53% Activity at 2.0625 uM_third_point_CF-PAF (2.0625μM**)% Activity at the test concentrationFloat%
54% Activity at 1.03125 uM_third_point_CF-PAF (1.03125μM**)% Activity at the test concentrationFloat%
55% Activity at 0.515625 uM_third_point_CF-PAF (0.515625μM**)% Activity at the test concentrationFloat%
56% Activity at 0.2578125 uM_third_point_CF-PAF (0.257812μM**)% Activity at the test concentrationFloat%
57% Activity at 0.1289062 uM_third_point_CF-PAF (0.128906μM**)% Activity at the test concentrationFloat%
58IC50_Qualifier_4_CF-PAFThis qualifier is to be used with the next TID, IC50_4_CF-PAF. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
59IC50_4_CF-PAF*IC50 value determined using a sigmoidal dose response equation5cystic fibrosis transmembrane conductance regulator [Homo sapiens]FloatμM
60Std.Err(IC50)_4_CF-PAFStandard Error of the IC50 valueFloatμM
61nH_4_CF-PAFHill coefficient determined using sigmoidal dose response equationFloat
62Excluded_Points_fourth_point_CF-PAFFlags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
63% Activity at 66 uM_fourth_point_CF-PAF (66μM**)% Activity at the test concentrationFloat%
64% Activity at 33 uM_fourth_point_CF-PAF (33μM**)% Activity at the test concentrationFloat%
65% Activity at 16.5 uM_fourth_point_CF-PAF (16.5μM**)% Activity at the test concentrationFloat%
66% Activity at 8.25 uM_fourth_point_CF-PAF (8.25μM**)% Activity at the test concentrationFloat%
67% Activity at 4.125 uM_fourth_point_CF-PAF (4.125μM**)% Activity at the test concentrationFloat%
68% Activity at 2.0625 uM_fourth_point_CF-PAF (2.0625μM**)% Activity at the test concentrationFloat%
69% Activity at 1.03125 uM_fourth_point_CF-PAF (1.03125μM**)% Activity at the test concentrationFloat%
70% Activity at 0.515625 uM_fourth_point_CF-PAF (0.515625μM**)% Activity at the test concentrationFloat%
71% Activity at 0.2578125 uM_fourth_point_CF-PAF (0.257812μM**)% Activity at the test concentrationFloat%
72% Activity at 0.1289062 uM_fourth_point_CF-PAF (0.128906μM**)% Activity at the test concentrationFloat%
73IC50_Qualifier_5_CF-PAFThis qualifier is to be used with the next TID, IC50_5_CF-PAF. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
74IC50_5_CF-PAF*IC50 value determined using a sigmoidal dose response equation6cystic fibrosis transmembrane conductance regulator [Homo sapiens]FloatμM
75Std.Err(IC50)_5_CF-PAFStandard Error of the IC50 valueFloatμM
76nH_5_CF-PAFHill coefficient determined using sigmoidal dose response equationFloat
77Excluded_Points_fifth_point_CF-PAFFlags to indicate which of the fifth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
78% Activity at 66 uM_fifth_point_CF-PAF (66μM**)% Activity at the test concentrationFloat%
79% Activity at 33 uM_fifth_point_CF-PAF (33μM**)% Activity at the test concentrationFloat%
80% Activity at 16.5 uM_fifth_point_CF-PAF (16.5μM**)% Activity at the test concentrationFloat%
81% Activity at 8.25 uM_fifth_point_CF-PAF (8.25μM**)% Activity at the test concentrationFloat%
82% Activity at 4.125 uM_fifth_point_CF-PAF (4.125μM**)% Activity at the test concentrationFloat%
83% Activity at 2.0625 uM_fifth_point_CF-PAF (2.0625μM**)% Activity at the test concentrationFloat%
84% Activity at 1.03125 uM_fifth_point_CF-PAF (1.03125μM**)% Activity at the test concentrationFloat%
85% Activity at 0.515625 uM_fifth_point_CF-PAF (0.515625μM**)% Activity at the test concentrationFloat%
86% Activity at 0.2578125 uM_fifth_point_CF-PAF (0.257812μM**)% Activity at the test concentrationFloat%
87% Activity at 0.1289062 uM_fifth_point_CF-PAF (0.128906μM**)% Activity at the test concentrationFloat%
88IC50_Qualifier_6_CF-PAFThis qualifier is to be used with the next TID, IC50_6_CF-PAF. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
89IC50_6_CF-PAF*IC50 value determined using a sigmoidal dose response equation7cystic fibrosis transmembrane conductance regulator [Homo sapiens]FloatμM
90Std.Err(IC50)_6_CF-PAFStandard Error of the IC50 valueFloatμM
91nH_6_CF-PAFHill coefficient determined using sigmoidal dose response equationFloat
92Excluded_Points_sixth_point_CF-PAFFlags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
93% Activity at 66 uM_sixth_point_CF-PAF (66μM**)% Activity at the test concentrationFloat%
94% Activity at 33 uM_sixth_point_CF-PAF (33μM**)% Activity at the test concentrationFloat%
95% Activity at 16.5 uM_sixth_point_CF-PAF (16.5μM**)% Activity at the test concentrationFloat%
96% Activity at 8.25 uM_sixth_point_CF-PAF (8.25μM**)% Activity at the test concentrationFloat%
97% Activity at 4.125 uM_sixth_point_CF-PAF (4.125μM**)% Activity at the test concentrationFloat%
98% Activity at 2.0625 uM_sixth_point_CF-PAF (2.0625μM**)% Activity at the test concentrationFloat%
99% Activity at 1.03125 uM_sixth_point_CF-PAF (1.03125μM**)% Activity at the test concentrationFloat%
100% Activity at 0.515625 uM_sixth_point_CF-PAF (0.515625μM**)% Activity at the test concentrationFloat%
101% Activity at 0.2578125 uM_sixth_point_CF-PAF (0.257812μM**)% Activity at the test concentrationFloat%
102% Activity at 0.1289062 uM_sixth_point_CF-PAF (0.128906μM**)% Activity at the test concentrationFloat%
103IC50_Qualifier_1_PAF-IL8This qualifier is to be used with the next TID, IC50_1_PAF-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
104IC50_1_PAF-IL8*IC50 value determined using a sigmoidal dose response equation9interleukin-8 precursor [Homo sapiens]FloatμM
105Std.Err(IC50)_1_PAF-IL8Standard Error of the IC50 valueFloatμM
106nH_1_PAF-IL8Hill coefficient determined using sigmoidal dose response equationFloat
107Excluded_Points_first_point_PAF-IL8Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
108% Activity at 66 uM_first_point_PAF-IL8 (66μM**)% Activity at the test concentrationFloat%
109% Activity at 33 uM_first_point_PAF-IL8 (33μM**)% Activity at the test concentrationFloat%
110% Activity at 16.5 uM_first_point_PAF-IL8 (16.5μM**)% Activity at the test concentrationFloat%
111% Activity at 8.25 uM_first_point_PAF-IL8 (8.25μM**)% Activity at the test concentrationFloat%
112% Activity at 4.125 uM_first_point_PAF-IL8 (4.125μM**)% Activity at the test concentrationFloat%
113% Activity at 2.0625 uM_first_point_PAF-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
114% Activity at 1.03125 uM_first_point_PAF-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
115% Activity at 0.515625 uM_first_point_PAF-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
116% Activity at 0.2578125 uM_first_point_PAF-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
117% Activity at 0.1289062 uM_first_point_PAF-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
118IC50_Qualifier_2_PAF-IL8This qualifier is to be used with the next TID, IC50_2_PAF-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
119IC50_2_PAF-IL8*IC50 value determined using a sigmoidal dose response equation10interleukin-8 precursor [Homo sapiens]FloatμM
120Std.Err(IC50)_2_PAF-IL8Standard Error of the IC50 valueFloatμM
121nH_2_PAF-IL8Hill coefficient determined using sigmoidal dose response equationFloat
122Excluded_Points_second_point_PAF-IL8Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
123% Activity at 66 uM_second_point_PAF-IL8 (66μM**)% Activity at the test concentrationFloat%
124% Activity at 33 uM_second_point_PAF-IL8 (33μM**)% Activity at the test concentrationFloat%
125% Activity at 16.5 uM_second_point_PAF-IL8 (16.5μM**)% Activity at the test concentrationFloat%
126% Activity at 8.25 uM_second_point_PAF-IL8 (8.25μM**)% Activity at the test concentrationFloat%
127% Activity at 4.125 uM_second_point_PAF-IL8 (4.125μM**)% Activity at the test concentrationFloat%
128% Activity at 2.0625 uM_second_point_PAF-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
129% Activity at 1.03125 uM_second_point_PAF-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
130% Activity at 0.515625 uM_second_point_PAF-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
131% Activity at 0.2578125 uM_second_point_PAF-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
132% Activity at 0.1289062 uM_second_point_PAF-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
133IC50_Qualifier_3_PAF-IL8This qualifier is to be used with the next TID, IC50_3_PAF-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
134IC50_3_PAF-IL8*IC50 value determined using a sigmoidal dose response equation11interleukin-8 precursor [Homo sapiens]FloatμM
135Std.Err(IC50)_3_PAF-IL8Standard Error of the IC50 valueFloatμM
136nH_3_PAF-IL8Hill coefficient determined using sigmoidal dose response equationFloat
137Excluded_Points_third_point_PAF-IL8Flags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
138% Activity at 66 uM_third_point_PAF-IL8 (66μM**)% Activity at the test concentrationFloat%
139% Activity at 33 uM_third_point_PAF-IL8 (33μM**)% Activity at the test concentrationFloat%
140% Activity at 16.5 uM_third_point_PAF-IL8 (16.5μM**)% Activity at the test concentrationFloat%
141% Activity at 8.25 uM_third_point_PAF-IL8 (8.25μM**)% Activity at the test concentrationFloat%
142% Activity at 4.125 uM_third_point_PAF-IL8 (4.125μM**)% Activity at the test concentrationFloat%
143% Activity at 2.0625 uM_third_point_PAF-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
144% Activity at 1.03125 uM_third_point_PAF-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
145% Activity at 0.515625 uM_third_point_PAF-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
146% Activity at 0.2578125 uM_third_point_PAF-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
147% Activity at 0.1289062 uM_third_point_PAF-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
148IC50_Qualifier_4_PAF-IL8This qualifier is to be used with the next TID, IC50_4_PAF-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
149IC50_4_PAF-IL8*IC50 value determined using a sigmoidal dose response equation12interleukin-8 precursor [Homo sapiens]FloatμM
150Std.Err(IC50)_4_PAF-IL8Standard Error of the IC50 valueFloatμM
151nH_4_PAF-IL8Hill coefficient determined using sigmoidal dose response equationFloat
152Excluded_Points_fourth_point_PAF-IL8Flags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
153% Activity at 66 uM_fourth_point_PAF-IL8 (66μM**)% Activity at the test concentrationFloat%
154% Activity at 33 uM_fourth_point_PAF-IL8 (33μM**)% Activity at the test concentrationFloat%
155% Activity at 16.5 uM_fourth_point_PAF-IL8 (16.5μM**)% Activity at the test concentrationFloat%
156% Activity at 8.25 uM_fourth_point_PAF-IL8 (8.25μM**)% Activity at the test concentrationFloat%
157% Activity at 4.125 uM_fourth_point_PAF-IL8 (4.125μM**)% Activity at the test concentrationFloat%
158% Activity at 2.0625 uM_fourth_point_PAF-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
159% Activity at 1.03125 uM_fourth_point_PAF-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
160% Activity at 0.515625 uM_fourth_point_PAF-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
161% Activity at 0.2578125 uM_fourth_point_PAF-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
162% Activity at 0.1289062 uM_fourth_point_PAF-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
163IC50_Qualifier_5_PAF-IL8This qualifier is to be used with the next TID, IC50_5_PAF-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
164IC50_5_PAF-IL8*IC50 value determined using a sigmoidal dose response equation13interleukin-8 precursor [Homo sapiens]FloatμM
165Std.Err(IC50)_5_PAF-IL8Standard Error of the IC50 valueFloatμM
166nH_5_PAF-IL8Hill coefficient determined using sigmoidal dose response equationFloat
167Excluded_Points_fifth_point_PAF-IL8Flags to indicate which of the fifth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
168% Activity at 66 uM_fifth_point_PAF-IL8 (66μM**)% Activity at the test concentrationFloat%
169% Activity at 33 uM_fifth_point_PAF-IL8 (33μM**)% Activity at the test concentrationFloat%
170% Activity at 16.5 uM_fifth_point_PAF-IL8 (16.5μM**)% Activity at the test concentrationFloat%
171% Activity at 8.25 uM_fifth_point_PAF-IL8 (8.25μM**)% Activity at the test concentrationFloat%
172% Activity at 4.125 uM_fifth_point_PAF-IL8 (4.125μM**)% Activity at the test concentrationFloat%
173% Activity at 2.0625 uM_fifth_point_PAF-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
174% Activity at 1.03125 uM_fifth_point_PAF-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
175% Activity at 0.515625 uM_fifth_point_PAF-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
176% Activity at 0.2578125 uM_fifth_point_PAF-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
177% Activity at 0.1289062 uM_fifth_point_PAF-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
178IC50_Qualifier_6_PAF-IL8This qualifier is to be used with the next TID, IC50_6_PAF-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
179IC50_6_PAF-IL8*IC50 value determined using a sigmoidal dose response equation14interleukin-8 precursor [Homo sapiens]FloatμM
180Std.Err(IC50)_6_PAF-IL8Standard Error of the IC50 valueFloatμM
181nH_6_PAF-IL8Hill coefficient determined using sigmoidal dose response equationFloat
182Excluded_Points_sixth_point_PAF-IL8Flags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
183% Activity at 66 uM_sixth_point_PAF-IL8 (66μM**)% Activity at the test concentrationFloat%
184% Activity at 33 uM_sixth_point_PAF-IL8 (33μM**)% Activity at the test concentrationFloat%
185% Activity at 16.5 uM_sixth_point_PAF-IL8 (16.5μM**)% Activity at the test concentrationFloat%
186% Activity at 8.25 uM_sixth_point_PAF-IL8 (8.25μM**)% Activity at the test concentrationFloat%
187% Activity at 4.125 uM_sixth_point_PAF-IL8 (4.125μM**)% Activity at the test concentrationFloat%
188% Activity at 2.0625 uM_sixth_point_PAF-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
189% Activity at 1.03125 uM_sixth_point_PAF-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
190% Activity at 0.515625 uM_sixth_point_PAF-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
191% Activity at 0.2578125 uM_sixth_point_PAF-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
192% Activity at 0.1289062 uM_sixth_point_PAF-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
193IC50_Qualifier_1_TNFa-IL8This qualifier is to be used with the next TID, IC50_1_TNFa-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
194IC50_1_TNFa-IL8*IC50 value determined using a sigmoidal dose response equation16interleukin-8 precursor [Homo sapiens]FloatμM
195Std.Err(IC50)_1_TNFa-IL8Standard Error of the IC50 valueFloatμM
196nH_1_TNFa-IL8Hill coefficient determined using sigmoidal dose response equationFloat
197Excluded_Points_first_point_TNFa-IL8Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
198% Activity at 66 uM_first_point_TNFa-IL8 (66μM**)% Activity at the test concentrationFloat%
199% Activity at 33 uM_first_point_TNFa-IL8 (33μM**)% Activity at the test concentrationFloat%
200% Activity at 16.5 uM_first_point_TNFa-IL8 (16.5μM**)% Activity at the test concentrationFloat%
201% Activity at 8.25 uM_first_point_TNFa-IL8 (8.25μM**)% Activity at the test concentrationFloat%
202% Activity at 4.125 uM_first_point_TNFa-IL8 (4.125μM**)% Activity at the test concentrationFloat%
203% Activity at 2.0625 uM_first_point_TNFa-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
204% Activity at 1.03125 uM_first_point_TNFa-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
205% Activity at 0.515625 uM_first_point_TNFa-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
206% Activity at 0.2578125 uM_first_point_TNFa-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
207% Activity at 0.1289062 uM_first_point_TNFa-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
208IC50_Qualifier_2_TNFa-IL8This qualifier is to be used with the next TID, C50_2_TNFa-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
209IC50_2_TNFa-IL8*IC50 value determined using a sigmoidal dose response equation17interleukin-8 precursor [Homo sapiens]FloatμM
210Std.Err(IC50)_2_TNFa-IL8Standard Error of the IC50 valueFloatμM
211nH_2_TNFa-IL8Hill coefficient determined using sigmoidal dose response equationFloat
212Excluded_Points_second_point_TNFa-IL8Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
213% Activity at 66 uM_second_point_TNFa-IL8 (66μM**)% Activity at the test concentrationFloat%
214% Activity at 33 uM_second_point_TNFa-IL8 (33μM**)% Activity at the test concentrationFloat%
215% Activity at 16.5 uM_second_point_TNFa-IL8 (16.5μM**)% Activity at the test concentrationFloat%
216% Activity at 8.25 uM_second_point_TNFa-IL8 (8.25μM**)% Activity at the test concentrationFloat%
217% Activity at 4.125 uM_second_point_TNFa-IL8 (4.125μM**)% Activity at the test concentrationFloat%
218% Activity at 2.0625 uM_second_point_TNFa-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
219% Activity at 1.03125 uM_second_point_TNFa-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
220% Activity at 0.515625 uM_second_point_TNFa-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
221% Activity at 0.2578125 uM_second_point_TNFa-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
222% Activity at 0.1289062 uM_second_point_TNFa-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
223IC50_Qualifier_3_TNFa-IL8This qualifier is to be used with the next TID, IC50_3_TNFa-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
224IC50_3_TNFa-IL8*IC50 value determined using a sigmoidal dose response equation18interleukin-8 precursor [Homo sapiens]FloatμM
225Std.Err(IC50)_3_TNFa-IL8Standard Error of the IC50 valueFloatμM
226nH_3_TNFa-IL8Hill coefficient determined using sigmoidal dose response equationFloat
227Excluded_Points_third_point_TNFa-IL8Flags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
228% Activity at 66 uM_third_point_TNFa-IL8 (66μM**)% Activity at the test concentrationFloat%
229% Activity at 33 uM_third_point_TNFa-IL8 (33μM**)% Activity at the test concentrationFloat%
230% Activity at 16.5 uM_third_point_TNFa-IL8 (16.5μM**)% Activity at the test concentrationFloat%
231% Activity at 8.25 uM_third_point_TNFa-IL8 (8.25μM**)% Activity at the test concentrationFloat%
232% Activity at 4.125 uM_third_point_TNFa-IL8 (4.125μM**)% Activity at the test concentrationFloat%
233% Activity at 2.0625 uM_third_point_TNFa-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
234% Activity at 1.03125 uM_third_point_TNFa-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
235% Activity at 0.515625 uM_third_point_TNFa-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
236% Activity at 0.2578125 uM_third_point_TNFa-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
237% Activity at 0.1289062 uM_third_point_TNFa-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
238IC50_Qualifier_4_TNFa-IL8This qualifier is to be used with the next TID, IC50_4_TNFa-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
239IC50_4_TNFa-IL8*IC50 value determined using a sigmoidal dose response equation19interleukin-8 precursor [Homo sapiens]FloatμM
240Std.Err(IC50)_4_TNFa-IL8Standard Error of the IC50 valueFloatμM
241nH_4_TNFa-IL8Hill coefficient determined using sigmoidal dose response equationFloat
242Excluded_Points_fourth_point_TNFa-IL8Flags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
243% Activity at 66 uM_fourth_point_TNFa-IL8 (66μM**)% Activity at the test concentrationFloat%
244% Activity at 33 uM_fourth_point_TNFa-IL8 (33μM**)% Activity at the test concentrationFloat%
245% Activity at 16.5 uM_fourth_point_TNFa-IL8 (16.5μM**)% Activity at the test concentrationFloat%
246% Activity at 8.25 uM_fourth_point_TNFa-IL8 (8.25μM**)% Activity at the test concentrationFloat%
247% Activity at 4.125 uM_fourth_point_TNFa-IL8 (4.125μM**)% Activity at the test concentrationFloat%
248% Activity at 2.0625 uM_fourth_point_TNFa-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
249% Activity at 1.03125 uM_fourth_point_TNFa-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
250% Activity at 0.515625 uM_fourth_point_TNFa-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
251% Activity at 0.2578125 uM_fourth_point_TNFa-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
252% Activity at 0.1289062 uM_fourth_point_TNFa-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
253IC50_Qualifier_5_TNFa-IL8This qualifier is to be used with the next TID, IC50_5_TNFa-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
254IC50_5_TNFa-IL8*IC50 value determined using a sigmoidal dose response equation20interleukin-8 precursor [Homo sapiens]FloatμM
255Std.Err(IC50)_5_TNFa-IL8Standard Error of the IC50 valueFloatμM
256nH_5_TNFa-IL8Hill coefficient determined using sigmoidal dose response equationFloat
257Excluded_Points_fifth_point_TNFa-IL8Flags to indicate which of the fifth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
258% Activity at 66 uM_fifth_point_TNFa-IL8 (66μM**)% Activity at the test concentrationFloat%
259% Activity at 33 uM_fifth_point_TNFa-IL8 (33μM**)% Activity at the test concentrationFloat%
260% Activity at 16.5 uM_fifth_point_TNFa-IL8 (16.5μM**)% Activity at the test concentrationFloat%
261% Activity at 8.25 uM_fifth_point_TNFa-IL8 (8.25μM**)% Activity at the test concentrationFloat%
262% Activity at 4.125 uM_fifth_point_TNFa-IL8 (4.125μM**)% Activity at the test concentrationFloat%
263% Activity at 2.0625 uM_fifth_point_TNFa-IL8% Activity at the test concentrationFloat%
264% Activity at 1.03125 uM_fifth_point_TNFa-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
265% Activity at 0.515625 uM_fifth_point_TNFa-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
266% Activity at 0.2578125 uM_fifth_point_TNFa-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
267% Activity at 0.1289062 uM_fifth_point_TNFa-IL8 (0.128906μM**)% Activity at the test concentrationFloat%
268IC50_Qualifier_6_TNFa-IL8This qualifier is to be used with the next TID, IC50_6_TNFa-IL8. If the qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
269IC50_6_TNFa-IL8*IC50 value determined using a sigmoidal dose response equation21interleukin-8 precursor [Homo sapiens]FloatμM
270Std.Err(IC50)_6_TNFa-IL8Standard Error of the IC50 valueFloatμM
271nH_6_TNFa-IL8Hill coefficient determined using sigmoidal dose response equationFloat
272Excluded_Points_sixth_point_TNFa-IL8Flags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
273% Activity at 66 uM_sixth_point_TNFa-IL8 (66μM**)% Activity at the test concentrationFloat%
274% Activity at 33 uM_sixth_point_TNFa-IL8 (33μM**)% Activity at the test concentrationFloat%
275% Activity at 16.5 uM_sixth_point_TNFa-IL8 (16.5μM**)% Activity at the test concentrationFloat%
276% Activity at 8.25 uM_sixth_point_TNFa-IL8% Activity at the test concentrationFloat%
277% Activity at 4.125 uM_sixth_point_TNFa-IL8 (4.125μM**)% Activity at the test concentrationFloat%
278% Activity at 2.0625 uM_sixth_point_TNFa-IL8 (2.0625μM**)% Activity at the test concentrationFloat%
279% Activity at 1.03125 uM_sixth_point_TNFa-IL8 (1.03125μM**)% Activity at the test concentrationFloat%
280% Activity at 0.515625 uM_sixth_point_TNFa-IL8 (0.515625μM**)% Activity at the test concentrationFloat%
281% Activity at 0.2578125 uM_sixth_point_TNFa-IL8 (0.257812μM**)% Activity at the test concentrationFloat%
282% Activity at 0.1289062 uM_sixth_point_TNFa-IL8 (0.128906μM**)% Activity at the test concentrationFloat%

* Activity Concentration. ** Test Concentration. § Panel component ID.
Additional Information
Grant Number: IR21 NS061743-01

Classification
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