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BioAssay: AID 624363

MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_Activity_Set5

The primary HTS will be performed using SK-MEL-5 melanoma cells stably expressing a construct that contains the TRPM1 promoter upstream of a luciferase reporter gene. TRPM-1 is a known target of the transcription factor, microphtalmia-associated transcription factor (MITF). The assay in its current format utilizes plating of 2000 cells per well in 384 well plates using 30 mul total volume (cells more ..
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 Tested Compounds
 Tested Compounds
All(119)
 
 
Active(112)
 
 
Inactive(6)
 
 
Inconclusive(1)
 
 
 Tested Substances
 Tested Substances
All(122)
 
 
Active(115)
 
 
Inactive(6)
 
 
Inconclusive(1)
 
 
 Related BioAssays
 Related BioAssays
AID: 624363
Data Source: Broad Institute (2084-01_Inhibitor_Dose_DryPowder_Activity_Set5)
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Depositor Category: NIH Molecular Libraries Probe Production Network
Deposit Date: 2012-07-09
Hold-until Date: 2013-07-02
Modify Date: 2013-07-02

Data Table ( Complete ):           View Active Data    View All Data
BioActive Compounds: 112
Related Experiments
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AIDNameTypeProbeComment
488944Summary of Broad Institute MLPCN Identification of MITF Inhibitors ProjectSummary1 depositor-specified cross reference: Summary assay
488899MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_SinglePoint_HTS_ActivityScreening same project related to Summary assay
493073MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_DoseNoFile_CherryPick_Activity_Set3Confirmatory same project related to Summary assay
493102MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_DoseNoFile_CherryPick_Activity_Set4Confirmatory same project related to Summary assay
493177MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_DoseNoFile_CherryPick_Activity_Set2Confirmatory same project related to Summary assay
493191MITF Orthogonal Assay: MALME3 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-04_Inhibitor_Dose_CherryPick_ActivityConfirmatory same project related to Summary assay
493240MITF Orthogonal Assay: SK-MEL-5 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-02_Inhibitor_Dose_CherryPick_Activity_Set3Confirmatory same project related to Summary assay
540335MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_CherryPick_Activity_Set2Confirmatory same project related to Summary assay
540339MITF Orthogonal Assay: MALME3 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-04_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
540346MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
540347MITF Orthogonal Assay: SK-MEL-5 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-02_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
540348MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
624259MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_Activity_Set3Confirmatory same project related to Summary assay
624289MITF Orthogonal Assay: SK-MEL-5 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-02_Inhibitor_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
624290MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
624299MITF Orthogonal Assay: MALME3 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-04_Inhibitor_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
624315MITF Orthogonal Assay: SK-MEL-5 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-02_Inhibitor_Dose_DryPowder_Activity_Set3Confirmatory same project related to Summary assay
624316MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_Activity_Set4Confirmatory same project related to Summary assay
624324MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_DryPowder_Activity_Set3Confirmatory same project related to Summary assay
624362MITF Orthogonal Assay: MALME3 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-04_Inhibitor_Dose_DryPowder_Activity_Set3Confirmatory same project related to Summary assay
624364MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_DryPowder_Activity_Set4Confirmatory same project related to Summary assay
624366MITF Orthogonal Assay: SK-MEL-5 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-02_Inhibitor_Dose_DryPowder_Activity_Set4Confirmatory same project related to Summary assay
624368MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_DryPowder_Activity_Set5Confirmatory same project related to Summary assay
624426MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_Activity_Set7Confirmatory same project related to Summary assay
624427MITF Orthogonal Assay: SK-MEL-5 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-02_Inhibitor_Dose_DryPowder_Activity_Set5Confirmatory same project related to Summary assay
624428MITF Orthogonal Assay: SK-MEL-5 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-02_Inhibitor_Dose_DryPowder_Activity_Set7Confirmatory same project related to Summary assay
624429MITF Orthogonal Assay: SK-MEL-5 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-02_Inhibitor_Dose_DryPowder_Activity_Set6Confirmatory same project related to Summary assay
624430MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_Activity_Set8Confirmatory same project related to Summary assay
624440MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_Activity_Set6Confirmatory same project related to Summary assay
624488MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_DryPowder_Activity_Set6Confirmatory same project related to Summary assay
624489MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_DryPowder_Activity_Set2Confirmatory same project related to Summary assay
624490MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_DryPowder_Activity_Set7Confirmatory same project related to Summary assay
624492MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_DryPowder_Activity_Set8Confirmatory same project related to Summary assay
651584MITF Orthogonal Assay: MALME3 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-04_Inhibitor_Dose_DryPowder_Activity_Set5Confirmatory same project related to Summary assay
651585MITF Orthogonal Assay: MALME3 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-04_Inhibitor_Dose_DryPowder_Activity_Set6Confirmatory same project related to Summary assay
651586MITF Orthogonal Assay: SK-MEL-5 CTG Assay Measured in Cell-Based System Using Plate Reader - 2084-02_Inhibitor_Dose_DryPowder_Activity_Set8Confirmatory same project related to Summary assay
651588MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_Activity_Set9Confirmatory same project related to Summary assay
651591MITF: Counter assay: A375 proliferation Measured in Cell-Based System Using Plate Reader - 2084-03_Inhibitor_Dose_DryPowder_Activity_Set9Confirmatory same project related to Summary assay
651753MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_Dose_DryPowder_Activity_Set10Confirmatory same project related to Summary assay
651770qPCR Assay for TRPM1 Expression Measured in Cell-Based System Using RT-PCR - 2084-09_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651771qPCR Assay for DCT Expression Measured in Cell-Based System Using RT-PCR - 2084-12_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651772qPCR Assay for CDK2 Expression Measured in Cell-Based System Using RT-PCR - 2084-11_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651773qPCR Assay for MITF Expression Measured in Cell-Based System Using RT-PCR - 2084-05_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651795qPCR Assay for MLANA Expression Measured in Cell-Based System Using RT-PCR - 2084-13_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
651920Primary human melanocyte cytotoxicity assay Measured in Cell-Based System Using Plate Reader - 2084-06_Inhibitor_Dose_DryPowder_ActivityConfirmatory same project related to Summary assay
Description:
Keywords: MITF, TRPM-1, melanoma, SK-MEL-5, luciferase reporter

Assay Overview:
The primary HTS will be performed using SK-MEL-5 melanoma cells stably expressing a construct that contains the TRPM1 promoter upstream of a luciferase reporter gene. TRPM-1 is a known target of the transcription factor, microphtalmia-associated transcription factor (MITF). The assay in its current format utilizes plating of 2000 cells per well in 384 well plates using 30 mul total volume (cells plus media) per well. This will allow us to use a volume of 100 nl for pinning of the compounds 24 hours later. The cells will be incubated with test molecules for 24 hours and the luciferase activity will be detected using 20 mul per well of the Steady Glo Reagent from Perkin Elmer. The positive control is 27 uM parthenolide which is known to decrease TRPM-1 expression and kill melanoma cells.

Expected outcome:
Compounds that inhibit MITF directly or indirectly will reduce the expression of TRPM-1 expression and lead to a reduction in luminescence signal. Compounds that increase the activity of MITF or somehow lead to increased expression of TRPM-1 will lead to an increase in luminescence signal.
Protocol
MITF Primary Screening Protocol
(TRPM-1 Promoter//Luciferase reporter assay)

Day 1, plate TRPM-1 luc/SKMEL5 cells at 2000 per well in 30 uL media (phenol red free DMEM/10% heat inactivated Fetal Bovine Serum/Penicillin/Streptomycin/L-Glutamine)

Day 2, pin 100 nL compound/DMSO solution into 30 uL assay volume in white, opaque Corning 8867 barcoded 384 well plates. (For HTS, will require sentinel pinning with the positive control, parthenolide)
Incubate 24 hours at 37 degrees C in Liconic incubator.

Day 3, add 20 uL 100% Promega SteadyGlo per well with Thermo Combi fluid transfer apparatus.
Shake 15 seconds on "big bear" plate shaker and incubate at room temperature for 5 minutes.
Read on the Perkin-Elmer EnVision plate reader with ultra-sensitive luminescence (US LUM) settings for 0.5 sec per well
Comment
PRESENCE OF CONTROLS: Neutral control wells (NC; n=104) and positive control wells (PC; n=20) were included on every plate.

EXPECTED OUTCOME: Active compounds result in decreasing readout signal.
The compounds were assayed in multiple independent instances using an identical protocol; each instance is called a 'test'. For each test, the following analysis was applied:

ACTIVE CONCENTRATION LIMIT:
For each sample, the highest valid tested concentration (Max_Concentration) was determined and the active concentration limit (AC_limit) was set to equal (2)(Max_Concentration).

NORMALIZATION:
The raw signals of the plate wells were normalized using the 'Neutral Controls Minus Inhibitors' method in Genedata Assay Analyzer (v7.0.3):
The median raw signal of the intraplate neutral control wells was set to a normalized activity value of 0.
The median raw signal of the intraplate positive control wells was set to a normalized activity value of -100.
Experimental wells values were scaled to this range.

PATTERN CORRECTION: No plate pattern correction algorithm from Genedata Condoseo (v.7.0.3) was applied.

MEASUREMENT USED TO DETERMINE ACTIVE CONCENTRATION (AC): AC50
AC values were calculated using the curve fitting strategies in Genedata Screener Condoseo (7.0.3).
AC values were calculated up to the active concentration limit described for each sample.
pAC was set to equal -1*log10(AC)

PUBCHEM_ACTIVITY_OUTCOME:
Activity_Outcome = 1 (inactive) when:
a) compound shows activity but in a direction opposite to the expected outcome
in these cases, values describing curve fitting parameters (Sinf, S0, Hill Slope, log_AC50, log_AC50_SE) are set to null
b) curve fit is constant where activity is > -30% and < 30% at all tested concentrations, or
c) AC > AC_limit
Activity_Outcome = 2 (active) when:
AC <= AC_limit
Activity_Outcome = 3 (inconclusive) when:
a) Curve fitting strategy resulted in a constant fit with activity >= -70% but <= -30%, or
b) The fit was deemed not valid due to poor fit quality.

PUBCHEM_ACTIVITY_SCORE:
If PUBCHEM_ACTIVITY_OUTCOME = 1 (inactive) or 3 (inconclusive),
then PUBCHEM_ACTIVITY_SCORE = 0
If PUBCHEM_ACTIVITY_OUTCOME = 2 (active)
then PUBCHEM_ACTIVITY_SCORE = (10)(pAC)
Scores relate to AC in this manner:
120 = 1 pM
90 = 1 nM
60 = 1 uM
30 = 1 mM
0 = 1 M
When the active concentration (AC) is calculated to be greater than the highest valid tested concentration (Max_Concentration), the PUBCHEM_ACTIVITY_SCORE is calculated using Max_Concentration as the basis.
When the active concentration (AC) is calculated to be less than the lowest tested concentration, the PUBCHEM_ACTIVITY_SCORE is calculated using the lowest tested concentration as the basis.

DATA AGGREGATION:
Once the data for each test was processed, the test number was appended to all column headers in that test's data set. The individual test results were then aggregated as follows:

1. The final PUBCHEM_ACTIVITY_OUTCOME was set to:
a. '2' (active) when all test outcomes were '2' (active), or
b. '1' (inactive) when all test outcomes were '1' (inactive), or
c. '3' (inconclusive) when the test outcomes were mixed.

2. The final ACTIVE_CONCENTRATION (AC) was set as follows:
a. If the final PUBCHEM_ACTIVITY_OUTCOME = 2, AC was set as the mean of the constituent test active concentrations;
b. If the final PUBCHEM_ACTIVITY_OUTCOME = 1 or 3, AC was left empty.

3. The final PUBCHEM_ACTIVITY_SCORE was calculated based on the aggregated ACTIVE_CONCENTRATION, using the same logic described above for individual test scores.

Note:
The individual dose data point columns ('Activity_at_xxuM') reported here represent the median of valid (unmasked) replicate observations at each concentration. These values are the inputs to a curve fitting algorithm.
All other data columns represent values which are derived during the curve fitting algorithm; this may sometimes include automatic further masking of some replicate data points.
Occasionally this results in perceived inconsistencies: for example, between the derived 'Maximal_Activity' and the apparent most active data point.
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1AC50_Qualifier'>','=', or '<'String
2AC50_uM*The concentration at which activity reaches 50% of the maximumFloatμM
3pAC50_MEqual to -1*log10(AC50)String
4ACTIVITY_OUTCOME_TEST1The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
5ACTIVITY_SCORE_TEST1The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
6ASSAYDATA_COMMENT_TEST1Comments relevant to the subset of data included in the indicated Test number.String
7AC50_Qualifier_TEST1'>','=', or '<'String
8AC50_uM_TEST1The concentration at which activity reaches 50% of the maximumFloatμM
9pAC50_M_TEST1Equal to -1*log10(AC50)String
10Hill_Slope_TEST1The slope at AC50Float
11S0_(%)_TEST1The fitted activity value at zero concentrationFloat%
12Sinf_(%)_TEST1The fitted activity value at infinite concentrationFloat%
13Num_Points_TEST1The number of data points used to generate the plotInteger
14Max_Activity_(%)_TEST1The maximum activity value observed, based on mean of replicates per concentrationFloat%
15Max_Activity_Conc_uM_TEST1The concentration at which the maximum activity is observedFloatμM
16Max_Concentration_uM_TEST1Maximum valid test concentrationFloatμM
17Activity_at_0.000060uM_(%)_TEST1 (6e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
18Activity_at_0.00012uM_(%)_TEST1 (0.00012μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
19Activity_at_0.00026uM_(%)_TEST1 (0.00026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
20Activity_at_0.0005uM_(%)_TEST1 (0.0005μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
21Activity_at_0.001uM_(%)_TEST1 (0.001μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
22Activity_at_0.0021uM_(%)_TEST1 (0.0021μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
23Activity_at_0.0042uM_(%)_TEST1 (0.0042μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
24Activity_at_0.008uM_(%)_TEST1 (0.008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
25Activity_at_0.016uM_(%)_TEST1 (0.016μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
26Activity_at_0.03uM_(%)_TEST1 (0.03μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
27Activity_at_0.068uM_(%)_TEST1 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
28Activity_at_0.135uM_(%)_TEST1 (0.135μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
29Activity_at_0.26uM_(%)_TEST1 (0.26μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
30Activity_at_0.5uM_(%)_TEST1 (0.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
31Activity_at_1uM_(%)_TEST1 (1μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
32Activity_at_2.1uM_(%)_TEST1 (2.1μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
33Activity_at_4.2uM_(%)_TEST1 (4.2μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
34Activity_at_8uM_(%)_TEST1 (8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
35Activity_at_16uM_(%)_TEST1 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
36Activity_at_19.5uM_(%)_TEST1 (19.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
37Activity_at_23.5uM_(%)_TEST1 (23.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
38Activity_at_26uM_(%)_TEST1 (26μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
39Activity_at_35uM_(%)_TEST1 (35μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
40ACTIVITY_OUTCOME_TEST2The PubChem Activity Outcome for the subset of data included in the indicated Test number.Integer
41ACTIVITY_SCORE_TEST2The PubChem Activity Score for the subset of data included in the indicated Test number.Integer
42ASSAYDATA_COMMENT_TEST2Comments relevant to the subset of data included in the indicated Test number.String
43AC50_Qualifier_TEST2'>','=', or '<'String
44AC50_uM_TEST2The concentration at which activity reaches 50% of the maximumFloatμM
45pAC50_M_TEST2Equal to -1*log10(AC50)String
46Hill_Slope_TEST2The slope at AC50Float
47S0_(%)_TEST2The fitted activity value at zero concentrationFloat%
48Sinf_(%)_TEST2The fitted activity value at infinite concentrationFloat%
49Num_Points_TEST2The number of data points used to generate the plotInteger
50Max_Activity_(%)_TEST2The maximum activity value observed, based on mean of replicates per concentrationFloat%
51Max_Activity_Conc_uM_TEST2The concentration at which the maximum activity is observedFloatμM
52Max_Concentration_uM_TEST2Maximum valid test concentrationFloatμM
53Activity_at_0.000060uM_(%)_TEST2 (6e-05μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
54Activity_at_0.00012uM_(%)_TEST2 (0.00012μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
55Activity_at_0.00026uM_(%)_TEST2 (0.00026μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
56Activity_at_0.0005uM_(%)_TEST2 (0.0005μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
57Activity_at_0.001uM_(%)_TEST2 (0.001μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
58Activity_at_0.0021uM_(%)_TEST2 (0.0021μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
59Activity_at_0.0042uM_(%)_TEST2 (0.0042μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
60Activity_at_0.008uM_(%)_TEST2 (0.008μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
61Activity_at_0.016uM_(%)_TEST2 (0.016μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
62Activity_at_0.03uM_(%)_TEST2 (0.03μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
63Activity_at_0.068uM_(%)_TEST2 (0.068μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
64Activity_at_0.135uM_(%)_TEST2 (0.135μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
65Activity_at_0.26uM_(%)_TEST2 (0.26μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
66Activity_at_0.5uM_(%)_TEST2 (0.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
67Activity_at_1uM_(%)_TEST2 (1μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
68Activity_at_2.1uM_(%)_TEST2 (2.1μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
69Activity_at_4.2uM_(%)_TEST2 (4.2μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
70Activity_at_8uM_(%)_TEST2 (8μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
71Activity_at_16uM_(%)_TEST2 (16μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
72Activity_at_19.5uM_(%)_TEST2 (19.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
73Activity_at_23.5uM_(%)_TEST2 (23.5μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
74Activity_at_26uM_(%)_TEST2 (26μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%
75Activity_at_35uM_(%)_TEST2 (35μM**)The average measured activity of all accepted replicates at the specified concentrationFloat%

* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: 1 R03 DA031089-01

Data Table (Concise)
Data Table ( Complete ):     View Active Data    View All Data
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