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BioAssay: AID 623869

Development of Subtype-specific Activators of the GIRK family of Potassium Channels (rmGlu8_Gqi9_Counterscreen)

GIRK potassium channels are a family of inward-rectifying potassium channels also known as the Kir3 family. They are expressed as homo and heterotetramers in with specific subunit combinations expressed in different brain regions and peripheral organ systems notably including the heart. Multiple lines of evidence support important roles for GIRK in a variety of physiological processes including more ..
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 Tested Compounds
 Tested Compounds
All(2114)
 
 
Active(76)
 
 
Inactive(2038)
 
 
 Tested Substances
 Tested Substances
All(2114)
 
 
Active(76)
 
 
Inactive(2038)
 
 
AID: 623869
Data Source: Vanderbilt Screening Center for GPCRs, Ion Channels and Transporters (rmGlu8_Gqi9_Ca2+_flux (Counterscreen))
Depositor Category: NIH Molecular Libraries Probe Production Network
BioAssay Version:
Deposit Date: 2012-03-27
Hold-until Date: 2013-03-24
Modify Date: 2013-03-26

Data Table ( Complete ):           View Active Data    View All Data
Target
BioActive Compounds: 76
Related Experiments
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AIDNameTypeProbeComment
488969Assay for HTS of Gi/Go-linked GPCRs using mGluR8: Primary ScreeningScreening depositor-specified cross reference: mGlu8 Primary Screening Assay
504480Assay for HTS of Gi/Go-linked GPCRs using mGluR8: SummarySummary depositor-specified cross reference: mGlu8 Summary Assay
623868Development of Subtype-specific Activators of the GIRK family of Potassium Channels (mGlu8_nonGIRK_Counterscreen)Other depositor-specified cross reference: mGlu8_nonGIRK_Counterscreen
623909Activators of the GIRK family of Potassium Channels (GIRK_Confirmatory_CRC)Confirmatory depositor-specified cross reference: rmGlu8_hGIRK1/2_CRC (GIRK_Confirmatory_CRC) - Dry powder reorder
623911Activators of the GIRK family of Potassium Channels (GIRK1/2_Confirmatory)Other depositor-specified cross reference: hGIRK1/2_mGlu8_WaveformData (GIRK1/2_Confirmatory)
623952Activators of the GIRK family of Potassium Channels (minus_mGlu8_GIRK_Confirmatory_CRC)Confirmatory depositor-specified cross reference
623974Activators of the GIRK family of Potassium Channels (GIRK2/3_Confirmatory_EP)Confirmatory depositor-specified cross reference
623975Activators of the GIRK family of Potassium Channels (GIRK1/4_ThalliumFlux_CRC)Confirmatory depositor-specified cross reference
623976Activators of the GIRK family of Potassium Channels (GIRK_1_4_Confirmatory_EP)Confirmatory1 depositor-specified cross reference
623977Activators of the GIRK family of Potassium Channels (GIRK_1_2_Confirmatory_EP)Confirmatory1 depositor-specified cross reference
623983Activators of the GIRK family of Potassium Channels (GIRK2/3_ThalliumFlux_CRC)Confirmatory depositor-specified cross reference
623984Activators of the GIRK family of Potassium Channels (hKir2.1_ThalliumFlux_CRC)Confirmatory depositor-specified cross reference
623986Activators of the GIRK family of Potassium Channels (hKV7.4_ThalliumFlux_CRC)Confirmatory depositor-specified cross reference
623988Activators of the GIRK family of Potassium Channels (hERG_ThalliumFlux_CRC)Confirmatory depositor-specified cross reference
624042Activators of the GIRK family of Potassium Channels (GIRK1/2_PTX_Treated_CRC)Confirmatory depositor-specified cross reference
623932ML297 Competition in Radioligand Binding assays (Ricerca)Other same project related to Summary assay
Description:
BACKGROUND:
GIRK potassium channels are a family of inward-rectifying potassium channels also known as the Kir3 family. They are expressed as homo and heterotetramers in with specific subunit combinations expressed in different brain regions and peripheral organ systems notably including the heart. Multiple lines of evidence support important roles for GIRK in a variety of physiological processes including the control of heart rate and electrical excitability in a variety of neuronal populations. Data concerning GIRK's role in neurons suggest GIRK as a potential target for a variety of therapeutic indications including pain, epilepsy, and reward/addiction. However, a complete lack of selective and effective GIRK activators has prevented any further target validation for the many indications where GIRK activation is speculated to be of potential benefit. And, although GIRK small molecule GIRK inhibitors are known, their lack of selectivity limits their utility as research tools or potential therapeutics for such indications as atrial fibrillation.

PURPOSE:
The purpose of this assay was to differentiate hits from the mGlu8 primary screen whose activity was likely mediated through mGlu8 versus directly through GIRK or via modulation of an undeclared thallium influx pathway. This assay takes advantage of mGlu8's ability to couple through the chimeric G-protein, Gqi9, to increases in intracellular calcium. The assay was run in parallel on the same day as the rmGlu8, hGIRK 1/2 thallium flux assay and the rmGlu8 thallium flux assay (see Data Analysis, below).
Protocol
High-throughput Screening:
Two days before assay, HEK-293 cells stably transfected with rmGlu8 were transiently transfected using standard lipid-based transfection methods with Gqi9 chimeric G-protein to enable coupling of mGlu8 to intracellular calcium flux. Twenty-thousand mGlu8-expressing cells/well transiently transfected Gqi9 were plated into 384-well, black-walled, clear-bottom, poly-D-lysine coated plates in 20 uL/well Dulbecco's Modified Eagles Medium (DMEM) supplemented with 10% (v/v) dialyzed fetal bovine serum and incubated overnight in a 5% CO2 incubator at 37 degrees C. Cell culture medium was removed from cell plates using a BioTek ELx405 cell washer and replaced with 20 uL/well 20 mM HEPES-buffered (pH 7.4) Hanks Balanced Salt Solution (HBSS) hereafter referred to as Assay Buffer. Twenty uL/well of 2 mM Fluo-4 AM in Assay Buffer was added to cell plates using a Thermo Fisher Combi. Cell plates were incubated for ~60 min at room temperature and then the Fluo-4 solution was removed from cell plates using the BioTek ELx405 cell washer and replaced with 20 uL/well Assay Buffer. Dye loaded and washed cell plates were transferred to a Hamamatsu FDSS 6000 and double-addition protocol was initiated. After 10 seconds, 20 uL/well of 20 uM test compound in 0.2% DMSO and Assay Buffer was added. After 4 minutes 10 uL/well of Assay Buffer containing an EC20 concentration of glutamate was added and 2 more minutes of data collection followed. Fluorescence data were collected at 1 Hz.

Data Analysis.
Waveform signals (fluorescence intensity versus time normalized by dividing each fluorescence value (F) by the initial fluorescence value for each trace (F0)) were examined by eye, side-by-side, on a well-per-well basis with waveform signals from the rmGlu8, hGIRK 1/2 thallium flux assay and the rmGlu8 thallium flux assay.

REFERENCES
1. Niswender C.M., Johnson K.A., Weaver C.D., Jones C.K, Luo Q., Rodriguez A.L., Marlo J.E., de Paulis T., Thompson A.D., Days E.L, Nalywajko T., Austin C.A., Williams M.B., Ayala J.E., Williams R., Lindsley C.W., P.J. Conn. (2008) Discovery, characterization, and antiparkinsonian effect of novel positive allosteric modulators of metabotropic glutamate receptor 4. Mol Pharmacol. 74(5):1345-88.
Comment
Scoring was conducted using the following codes: Inactive = 0; Agonist = 1, Positive Allosteric Modulator = 2; Inhibitor = -1; Fluorescent = 3; Baseline Increase = 4.

Compounds that showed activity in this assay and the rmGlu8, hGIRK 1/2 thallium flux assay but not the rmGlu8 thallium flux assay were set aside as potential mGlu8 modulators. Compounds that neither showed activity in this assay nor the rmGlu8 thallium flux assay but did show activity in the rmGlu8, hGIRK 1/2 thallium flux assay were taken forward as potential GIRK modulators.
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1Score_R1Manual score for R1 replicate run (Inactive = 0; Agonist = 1; Inhibitor = -1; Positive Modulator = 2; Fluorescent = 3; Baseline Increase = 4)Integer
2Score_R2Manual score for R2 replicate run (Inactive = 0; Agonist = 1; Inhibitor = -1; Positive Modulator = 2; Fluorescent = 3; Baseline Increase = 4)Integer
30_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
41000_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
52000_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
63000_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
74000_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
85014_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
96014_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
107014_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
118014_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
129014_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
1310014_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
1411014_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
1512014_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
1613014_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
1714030_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
1815030_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
1916030_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2017030_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2118030_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2219030_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2320030_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2421030_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2522030_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2623045_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2724045_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2825045_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
2926045_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3027045_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3128045_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3229045_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3330045_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3431045_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3532061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3633061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3734061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3835061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
3936061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4037061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4138061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4239061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4340061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4441061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4542061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4643061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4744061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4845061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
4946061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5047061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5148061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5249061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5350061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5451061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5552061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5653061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5754061_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5855076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
5956076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6057076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6158076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6259076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6360076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6461076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6562076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6663076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6764076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6865076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
6966076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7067076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7168076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7269076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7370076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7471076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7572076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7673076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7774076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7875076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
7976076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8077076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8178076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8279076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8380076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8481076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8582076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8683076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8784076_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8885077_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
8986078_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9087079_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9188080_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9289080_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9390080_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9491092_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9592092_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9693092_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9794092_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9895092_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
9996091_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
10097091_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
10198091_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
10299091_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
103100108_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
104101108_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
105102109_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
106103109_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
107104109_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
108105109_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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116113115_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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118115115_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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146143116_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
147144116_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
148145116_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
149146116_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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158155124_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
159156124_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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168165138_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
169166138_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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247244169_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
248245169_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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298295238_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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39895140_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
39996140_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
40097140_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
40198140_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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405102152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
406103151_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
407104151_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
408105150_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
409106150_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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412109150_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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414111150_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
415112150_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
416113150_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
417114149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
418115149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
419116149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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425122149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
426123149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
427124149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
428125149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
429126149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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431128149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
432129149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
433130149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
434131149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
435132149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
436133149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
437134149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
438135149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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442139149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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444141149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
445142149_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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447144148_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
448145148_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
449146148_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
450147148_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
451148148_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
452149153_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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454151152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
455152152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
456153152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
457154151_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
458155152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
459156152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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461158152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
462159152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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464161152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
465162152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
466163153_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
467164153_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
468165153_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
469166153_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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474171152_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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476173168_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
477174169_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
478175170_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
479176171_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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482179172_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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484181184_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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486183183_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
487184183_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
488185183_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
489186183_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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492189184_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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494191183_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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496193182_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
497194183_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
498195200_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
499196201_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
500197201_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
501198201_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
502199201_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
503200201_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
504201201_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
505202201_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
506203201_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
507204231_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
508205231_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
509206231_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
510207231_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
511208231_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
512209231_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
513210231_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
514211231_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
515212231_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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519216246_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
520217246_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
521218246_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
522219246_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
523220247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
524221247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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527224247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
528225247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
529226247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
530227247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
531228247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
532229247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
533230247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
534231247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
535232247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
536233247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
537234247_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
538235262_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
539236262_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
540237263_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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544241266_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
545242266_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
546243266_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
547244293_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
548245293_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
549246293_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
550247293_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
551248293_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
552249293_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
553250293_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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555252293_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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558255310_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
559256310_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
560257310_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
561258309_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
562259309_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
563260309_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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568265325_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
569266325_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
570267325_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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572269325_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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575272340_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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578275342_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
579276342_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
580277343_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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582279343_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
583280343_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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586283343_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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589286345_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
590287345_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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592289346_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
593290346_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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61012007_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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61416022_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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61618022_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
61719022_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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62426038_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
62527038_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
62628038_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
62729038_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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724126138_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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743145138_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
744146138_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
745147138_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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753155148_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
754156148_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
755157148_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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776179147_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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805208180_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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825228197_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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842245241_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
843246241_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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847250241_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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853256258_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
854257258_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
855258258_R1Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R1 replicate (per plate)Float
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1639226179_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
1640227178_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
1641228178_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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197813009_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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198318026_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
198419026_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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198621026_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
198722026_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
198823039_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
198924039_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
199025039_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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199530039_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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206196086_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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2203238193_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2204239193_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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2207242194_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2208243194_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2209244194_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2210245195_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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2212247195_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2213248195_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2214249195_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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2217252195_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2218253195_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2219254195_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2220255195_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
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2236271227_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2237272227_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2238273227_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)Float
2239274227_R2Fluorescence intensity normalized to baseline fluorescence (see Protocol) at time (micro-seconds) for R2 replicate (per plate)