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BioAssay: AID 602351

Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): LCMS-based animal-based Activity-Based Protein Profiling (ABPP) MudPIT selectivity analysis for ABHD6

Name: Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): LCMS-based animal-based Activity-Based Protein Profiling (ABPP) MudPIT selectivity analysis for ABHD6. ..more
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AID: 602351
Data Source: The Scripps Research Institute Molecular Screening Center (ABHD6_INH_LCMS_ABPPMUDPIT)
BioAssay Type: Panel
Depositor Category: NIH Molecular Libraries Probe Production Network, Assay Provider
BioAssay Version:
Deposit Date: 2012-03-09
Hold-until Date: 2012-10-12
Modify Date: 2012-10-12

Data Table ( Complete ):           View Active Data    View All Data
BioActive Compound: 1
Related Experiments
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AIDNameTypeProbeComment
504411Fluorescence-based primary biochemical high throughput screening assay to identify inhibitors of human diacylglycerol lipase, beta (DAGLB)Screening depositor-specified cross reference: Primary screen (DAGLB inhibitors in singlicate)
504420Summary of the probe development efforts to identify inhibitors of human diacylglycerol lipase, beta (DAGLB)Summary3 depositor-specified cross reference: Summary (DAGLB inhibitors)
504445Fluorescence-based biochemical high throughput confirmation assay for inhibitors of human diacylglycerol lipase, beta (DAGLB)Screening depositor-specified cross reference: ABPP screen (DAGLb inhibitors in triplicate)
602403Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of recombinant antitarget DAGLa in vitroOther depositor-specified cross reference
602415Assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): LC/MS-based biochemical inhibition of overexpressed DAGLb substrate turnover in vitroOther depositor-specified cross reference
624039Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based dose-response biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of antitarget ABHD6 in vitro, set 2Confirmatory depositor-specified cross reference
624041Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of mouse liver ABHD6 in vivoOther depositor-specified cross reference
624077Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of mouse liver ABHD6 in vivo upon oral compound administrationOther depositor-specified cross reference
624468Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): LCMS-based biochemical dose response assayConfirmatory depositor-specified cross reference
624472Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of DAGLb by enantiomers of KT116Other depositor-specified cross reference
602299Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of overexpressed DAGLb in vitro set 2Other same project related to Summary assay
602300Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of overexpressed DAGLb in vitro set 3Other same project related to Summary assay
602301Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of recombinant anti-target ABHD11 in vitro set 1Other same project related to Summary assay
602302Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of overexpressed DAGLb in vitro set 1Other same project related to Summary assay
602303Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of overexpressed DAGLb in vitro; triazole urea libraryOther same project related to Summary assay
602311Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of serine hydrolases in mouse brain membrane in vitro set 1Other same project related to Summary assay
602312Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of recombinant anti-target ABHD11 in vitro set 2Other same project related to Summary assay
602319Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of antitarget ABHD6 in vitroOther same project related to Summary assay
602320Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based dose-response biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of DAGLb in vitroConfirmatory same project related to Summary assay
602321Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of serine hydrolases in mouse brain membrane in vitro set 3Other same project related to Summary assay
602322Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based dose-response biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of antitarget ABHD6 in vitroConfirmatory same project related to Summary assay
602323Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of serine hydrolases in mouse brain membrane in vitro set 2Other same project related to Summary assay
602335Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based dose-response biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of anti-target ABHD6 in situConfirmatory same project related to Summary assay
602337Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): absorbance-based cell-based dose response assay to determine cytotoxicity of inhibitor compoundsConfirmatory same project related to Summary assay
602339Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): LCMS-based cell-based Activity-Based Protein Profiling (ABPP) SILAC selectivity analysisOther same project related to Summary assay
602341Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): LCMS-based cell-based Activity-Based Protein Profiling (ABPP) SILAC selectivity analysis for ABHD6Other same project related to Summary assay
602343Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of mouse brain ABHD6 in vivo, set 2Other same project related to Summary assay
602345Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of mouse macrophage DAGLb in vivoOther same project related to Summary assay
602347Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of mouse brain ABHD6 in vivoOther same project related to Summary assay
602349Late stage assay provider results from the probe development effort to identify inhibitors of DAGLb: fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) assay to distinguish systemic and peripheral inhibitorsOther same project related to Summary assay
602353Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): LCMS-based animal-based Activity-Based Protein Profiling (ABPP) MudPIT selectivity analysisOther same project related to Summary assay
602354Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical dose-response gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of DAGLb in situConfirmatory same project related to Summary assay
602355Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of serine hydrolases in vitroOther same project related to Summary assay
Description:
Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC)
Affiliation: The Scripps Research Institute, TSRI
Assay Provider: Benjamin Cravatt, The Scripps Research Institute (TSRI)
Network: Molecular Library Probe Production Centers Network (MLPCN)
Grant Proposal Number: 1 R01 DA025285
Grant Proposal PI: Benjamin Cravatt, The Scripps Research Institute (TSRI)
External Assay ID: ABHD6_INH_LCMS_ABPPMUDPIT

Name: Late stage assay provider results from the probe development effort to identify inhibitors of diacylglycerol lipase, beta (DAGLb): LCMS-based animal-based Activity-Based Protein Profiling (ABPP) MudPIT selectivity analysis for ABHD6.

Description:

Endocannabinoids (ECs) represent a unique group of lipids that function as chemical messengers in the nervous system. To date, the two principle ECs identified in mammals are N-arachidonoyl-ethanolamine (anandamide) and 2-arachidonoyl-glycerol (2-AG). They have been implicated in various physiological and pathological functions including appetite, pain, sensation, memory, and addiction (1). Unlike traditional neurotransmitters, which are stored in vesicles, ECs are synthesized and released on demand, and then rapidly degraded to terminate signaling. Thus, the metabolic pathways that govern EC turnover are critical in determining the magnitude and duration of neuronal signaling events (2). Endocannabinoid biosynthesis, in contrast to degradation, is poorly understood. Recently, two serine hydrolases, DAGL-a and DAGL-B, were cloned and found to selectively cleave sn-1 acyl chains from diacylglycerols (DAG) to generate 2-AG in vitro (3). Their function in the nervous system was validated in vivo by the generation of DAGL-a and DAGL-B knock-out mice (4, 5). However, it is still unclear to what extent DAGL-a/B catalytic activity contributes to 2-AG-mediated signaling. The development of potent and selective inhibitors would offer a means to perturb DAGL-a/B activity in a selective, reversible, and temporally-controlled manner. Given the non-selective nature of current DAGL-a/B inhibitors (6), specific chemical probes would serve as invaluable tools to delineate DAGL-a/B function in 2-AG signaling networks of the brain.

References:

1. Di Marzo, V. (2008) Targeting the endocannabinoid system: to enhance or reduce?, Nat Rev Drug Discov 7, 438-455.
2. Ahn, K., McKinney, M. K., and Cravatt, B. F. (2008) Enzymatic pathways that regulate endocannabinoid signaling in the nervous system, Chem Rev 108, 1687-1707.
3. Bisogno, T., Howell, F., Williams, G., Minassi, A., Cascio, M. G., Ligresti, A., Matias, I., Schiano-Moriello, A., Paul, P., Williams, E. J., Gangadharan, U., Hobbs, C., Di Marzo, V., and Doherty, P. (2003) Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain, J Cell Biol 163, 463-468.
4. Gao, Y., Vasilyev, D. V., Goncalves, M. B., Howell, F. V., Hobbs, C., Reisenberg, M., Shen, R., Zhang, M. Y., Strassle, B. W., Lu, P., Mark, L., Piesla, M. J., Deng, K., Kouranova, E. V., Ring, R. H., Whiteside, G. T., Bates, B., Walsh, F. S., Williams, G., Pangalos, M. N., Samad, T. A., and Doherty, P. (2010) Loss of Retrograde Endocannabinoid Signaling and Reduced Adult Neurogenesis in Diacylglycerol Lipase Knock-out Mice, J Neurosci 30, 2017-2024.
5. Tanimura, A., Yamazaki, M., Hashimotodani, Y., Uchigashima, M., Kawata, S., Abe, M., Kita, Y., Hashimoto, K., Shimizu, T., Watanabe, M., Sakimura, K., and Kano, M. (2010) The Endocannabinoid 2-Arachidonoylglycerol Produced by Diacylglycerol Lipase +/- Mediates Retrograde Suppression of Synaptic Transmission, Neuron 65, 320-327.
6. Hoover, H. S., Blankman, J. L., Niessen, S., and Cravatt, B. F. (2008) Selectivity of inhibitors of endocannabinoid biosynthesis evaluated by activity-based protein profiling, Bioorganic & Medicinal Chemistry Letters 18, 5838-5841.

Keywords:

late stage, late stage AID, assay provider, powders, counterscreen, diacylglycerol lipase, diacylglycerol lipase-beta, DAGL, DAGL-beta, DAGLB, hydrolase, serine hydrolase, appetite, pain, sensation, memory, addiction, abhydrolase domain containing protein 6, ABHD6, liquid chromatography, LC, tandem mass spectrometry, MS/MS, activity-based protein profiling, ABPP, multi-dimensional protein identification technology, MudPIT, fluorophosphonate-biotin, FP-biotin, inhibitor, inhibition, in vivo, Scripps, Scripps Research Institute Molecular Screening Center, SRIMSC, Molecular Libraries Probe Production Centers Network, MLPCN
Panel Information
Targets
    Data Table(Active)    Data Table(All)Show more
PID§NameSubstancePanel TargetsDescriptionAdditional Information
ActiveInactive
1KIAA13631neutral cholesterol ester hydrolase 1 [Mus musculus] [gi:30520239]
Taxonomy id: 10090
Gene id: 320024
2Apeh1Acylpeptide hydrolase [Mus musculus] [gi:19343726]
Taxonomy id: 10090
Gene id: 235606
3Ctsa1lysosomal protective protein isoform b precursor [Mus musculus] [gi:84042523]
Taxonomy id: 10090
Gene id: 19025
4Prcp1lysosomal Pro-X carboxypeptidase precursor [Mus musculus] [gi:33469015]
Taxonomy id: 10090
Gene id: 72461
5Lpl1lipoprotein lipase precursor [Mus musculus] [gi:126723006]
Taxonomy id: 10090
Gene id: 16956
6Lypla21acyl-protein thioesterase 2 [Mus musculus] [gi:7242156]
Taxonomy id: 10090
Gene id: 26394
7Pla2g151group XV phospholipase A2 precursor [Mus musculus] [gi:19527008]
Taxonomy id: 10090
Gene id: 192654
8Ctsg1cathepsin G preproprotein [Mus musculus] [gi:6681083]
Taxonomy id: 10090
Gene id: 13035
9Elane1neutrophil elastase precursor [Mus musculus] [gi:114052444]
Taxonomy id: 10090
Gene id: 50701
10Dpp91dipeptidyl peptidase 9 [Mus musculus] [gi:255003757]
Taxonomy id: 10090
Gene id: 224897
11Fasn1fatty acid synthase [Mus musculus] [gi:93102409]
Taxonomy id: 10090
Gene id: 14104
12Acot21acyl-coenzyme A thioesterase 2, mitochondrial precursor [Mus musculus] [gi:238624114]
Taxonomy id: 10090
Gene id: 171210
13Lypla11acyl-protein thioesterase 1 [Mus musculus] [gi:6678760]
Taxonomy id: 10090
Gene id: 18777
14Dpp81dipeptidyl peptidase 8 [Mus musculus] [gi:31542571]
Taxonomy id: 10090
Gene id: 74388
15Acot11acyl-coenzyme A thioesterase 1 [Mus musculus] [gi:6753550]
Taxonomy id: 10090
Gene id: 26897
16Pnpla71patatin-like phospholipase domain-containing protein 7 [Mus musculus] [gi:225007615]
Taxonomy id: 10090
Gene id: 241274
17Dpp41dipeptidyl peptidase 4 isoform 1 [Mus musculus] [gi:6753674]
Taxonomy id: 10090
Gene id: 13482
18Lipe1hormone-sensitive lipase isoform 2 [Mus musculus] [gi:87239972]
Taxonomy id: 10090
Gene id: 16890
19Abhd121monoacylglycerol lipase ABHD12 [Mus musculus] [gi:159110817]
Taxonomy id: 10090
Gene id: 76192
20Pla2g71platelet-activating factor acetylhydrolase precursor [Mus musculus] [gi:31980752]
Taxonomy id: 10090
Gene id: 27226
21Pafah1b21platelet-activating factor acetylhydrolase IB subunit beta [Mus musculus] [gi:40254624]
Taxonomy id: 10090
Gene id: 18475
22Pafah21platelet-activating factor acetylhydrolase 2, cytoplasmic [Mus musculus] [gi:225579137]
Taxonomy id: 10090
Gene id: 100163
23Magl1monoglyceride lipase isoform d [Mus musculus] [gi:261878511]
Taxonomy id: 10090
Gene id: 23945
24Daglb1sn1-specific diacylglycerol lipase beta [Mus musculus] [gi:31559956]
Taxonomy id: 10090
Gene id: 231871
25Pafah1b31platelet-activating factor acetylhydrolase IB subunit gamma [Mus musculus] [gi:6679201]
Taxonomy id: 10090
Gene id: 18476
26Dpp71dipeptidyl peptidase 2 precursor [Mus musculus] [gi:31981425]
Taxonomy id: 10090
Gene id: 83768
27Abhd101abhydrolase domain-containing protein 10, mitochondrial precursor [Mus musculus] [gi:269784760]
Taxonomy id: 10090
Gene id: 213012
28Aoah1acyloxyacyl hydrolase precursor [Mus musculus] [gi:6753064]
Taxonomy id: 10090
Gene id: 27052
29Pnpla61neuropathy target esterase isoform 1 [Mus musculus] [gi:390608665]
Taxonomy id: 10090
Gene id: 50767
30Abhd111abhydrolase domain-containing protein 11 isoform 1 [Mus musculus] [gi:21644577]
Taxonomy id: 10090
Gene id: 68758
31Tpp21Tpp2 protein [Mus musculus] [gi:37194903]
Taxonomy id: 10090
Gene id: 22019
32Fam108b1abhydrolase domain-containing protein FAM108B1 precursor [Mus musculus] [gi:38142456]
Taxonomy id: 10090
Gene id: 226016
33Iah11isoamyl acetate-hydrolyzing esterase 1 homolog [Mus musculus] [gi:27754071]
Taxonomy id: 10090
Gene id: 67732
34Prepl1prolyl endopeptidase-like isoform a [Mus musculus] [gi:254939518]
Taxonomy id: 10090
Gene id: 213760
35Abhd61monoacylglycerol lipase ABHD6 [Mus musculus] [gi:31560264]
Taxonomy id: 10090
Gene id: 66082
36Ppt21lysosomal thioesterase PPT2 precursor [Mus musculus] [gi:9506985]
Taxonomy id: 10090
Gene id: 54397
37Gzma1granzyme A precursor [Mus musculus] [gi:6754102]
Taxonomy id: 10090
Gene id: 14938
38Lipa1lysosomal acid lipase/cholesteryl ester hydrolase precursor [Mus musculus] [gi:162287343]
Taxonomy id: 10090
Gene id: 16889

§ Panel component ID.
Protocol
Assay Overview:
The purpose of this assay is to determine the selectivity profile of powder samples of test compounds in vivo by activity-based protein profiling (ABPP) in combination with the multidimensional protein identification technology (MudPIT) liquid chromatography tandem mass spectrometry (LC-M/MS) analysis platform. In this assay, mice are administered test compound or vehicle only. Mice are sacrificed, and primary macrophages removed, lysed, and reacted with the serine hydrolase specific activity-based fluorophosphonate-biotin (FP-biotin) affinity probe. Biotinylated proteins are enriched, trypsinized, and analyzed by MudPIT. Inhibition of target and anti-target activity is quantified by comparing spectra counts between the test compound- and DMSO-treated samples. As designed, compounds that act as inhibitors will block FP-biotin labeling, reducing enrichment in the inhibitor-treated sample relative to the DMSO-treated sample, thus giving a lower number of smaller spectra counts in the inhibitor treated sample. Proteins not targeted by inhibitors would be expected to have a spectra count ratio similar to DMSO control.
Protocol Summary:
Sample Preparation. Purpose-bred C57-black laboratory mice, injected 4 days prior with thioglycollate, were administered test compound KT195 (SID125269138) (5 mg/kg in 18:1:1 saline:PEG300:EtOH vehicle solution, i.p.) or vehicle only (n=3 per group). After 4 hours, mice were humanely sacrificed (anesthetized with isoflurane and decapitated) and primary macrophages were removed and snap frozen in liquid nitrogen. Tissues were homogenized and the protein concentration for each lysate was adjusted to 2 mg/mL with DPBS. The macrophage proteomes were labeled with the activity-based affinity probe FP-biotin (500 uL total reaction volume, 10 uM final concentration) for 2 hours at 25 C. After incubation, the proteomes were additionally solubilized with 1% Triton-X100. Samples were desalted over PD10 columns (GE Healthcare) in DPBS, and biotinylated proteins enriched with streptavidin beads (50 uL beads; conditions: 1 hour, 25 C 0.5% SDS in DPBS). The beads were washed with 1% SDS in DPBS (1x), 6 M urea (1x), and DPBS (2x), then resuspended in in 6 M urea (150 uL), reduced with 5 mM TCEP for 20 minutes, and alkylated with 10 mM iodoacetamide for 30 minutes at 25 C in the dark. The urea concentration was reduced to 2 M with 2x volume DPBS. On-bead digestions were performed for 12 hours at 37 C with sequence-grade modified trypsin (Promega; 2 ug) in the presence of 2 mM CaCl2. Peptide samples were acidified to a final concentration of 5% (v/v) formic acid and stored at -80 C prior to analysis.
LC-MS/MS analysis. Digested and acidified peptide mixtures were analyzed by two-dimensional liquid chromatography/tandem mass spectrometry (MudPIT) using an Agilent 1200-series quaternary pump and Thermo Scientific LTQ ion trap mass spectrometer. Peptides were eluted in a 5-step MudPIT experiment using 0%, 25%, 50%, 80%, and 100% salt bumps of 500 mM aqueous ammonium acetate, with chromatographic elution following each salt pulse accomplished using an increasing gradient of aqueous acetonitrile containing 0.1% formic acid over 125 minutes. Data were collected in data-dependent acquisition mode with dynamic exclusion turned on (90 s, repeat of 1). Specifically, one full MS (MS1) scan (400-1800 m/z) was followed by 7 MS2 scans of the most abundant ions. The MS2 spectra data were extracted from the raw file using RAW Xtractor (version 1.9.1; publically available at http://fields.scripps.edu/downloads.php). MS2 spectra data were searched using the PROLUCID algorithm (publically available at http://fields.scripps.edu/downloads.php) against the latest version of the mouse UniProt database concatenated with the reversed database for assessment of false-discovery rates. PROLUCID searches allowed for variable oxidation of methionine (+16), static modification of cysteine (+57 due to alkylation), and no enzyme specificity. The resulting MS2 spectra matches were assembled into protein identifications and filtered using DTASelect (version 2.0.41) using the -trypstat and -modstat options, which apply different statistical models for the analysis of tryptic, half-tryptic, non-tryptic, and modified peptides. DTASelect 2.0 uses a quadratic discriminant analysis to maintain a user-defined false positive hit rate (as determined by number of hits against the reversed database) below 1% at the peptide level. Spectra count abundance ratios (test compound/DMSO) were calculated for all serine hydrolases with an average of 4 or more spectra counts in the DMSO control samples. A Student's T-test (unpaired, equal variances) was utilized to determine statistical significance between the average spectra counts in compound vs. DMSO samples.
PubChem Activity Outcome and Score:
The following applies to each panel in this assay:
A compound was considered active for a particular target/anti-target if there was a statistical difference between the average spectra counts in the test compound- and DMSO-treated samples (P < 0.01). A compound was considered inactive for a specified target/anti-target if P >= 0.01.
Overall Outcome and Score:
A compound was considered active if it was active for ABHD6 and active for fewer than two anti-target serine hydrolases tested.
The PubChem Activity Score is assigned a value of 100 for active compounds, and 0 for inactive compounds.
The PubChem Activity Score range for active compounds is 100-100. There are no inactive compounds.
List of Reagents:
Purpose-bred WT laboratory mice (supplied by Assay Provider)
FP-biotin (provided by Assay Provider)
DPBS (Cellgro 20-031-CV)
Comment
This assay was performed by the assay provider with powder samples of synthetic compounds.
Categorized Comment - additional comments and annotations
From BioAssay Depositor:
BAO: assay format: biochemical format: protein format: single protein format
BAO: bioassay specification: assay biosafety level: bsl1
BAO: bioassay specification: assay measurement type: endpoint assay
BAO: bioassay specification: assay readout content: assay readout method: regular screening
BAO: bioassay specification: assay readout content: content readout type: single readout
BAO: bioassay specification: assay stage: secondary: counter screening
BAO: detection technology: fluorescence: fluorescence intensity
BAO: meta target detail: binding reporter specification: interaction: protein-small molecule
BAO: meta target: biological process target: regulation of molecular function
BAO: meta target: molecular target: protein target: enzyme: generic hydrolase
BAO: version: 1.4b1090
Result Definitions
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TIDNameDescriptionPID§Panel TargetsHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1Outcome [KIAA1363]One of Active, Inactive, or Not Tested1neutral cholesterol ester hydrolase 1 [Mus musculus]Outcome
2P value [KIAA1363]The P value determined from Student's t-test for vehicle vs. compound treated samples1Float
3Spectra counts, vehicle [1, KIAA1363]Number of spectra counts for KIAA1363 in vehicle treated sample; replicate [1]1Integer
4Spectra counts, vehicle [2, KIAA1363]Number of spectra counts for KIAA1363 in vehicle treated sample; replicate [2]1Integer
5Spectra counts, vehicle [3, KIAA1363]Number of spectra counts for KIAA1363 in vehicle treated sample; replicate [3]1Integer
6Spectra counts, test compound [1, KIAA1363]Number of spectra counts for KIAA1363 in compound treated sample; replicate [1]1Integer
7Spectra counts, test compound [2, KIAA1363]Number of spectra counts for KIAA1363 in compound treated sample; replicate [2]1Integer
8Spectra counts, test compound [3, KIAA1363]Number of spectra counts for KIAA1363 in compound treated sample; replicate [3]1Integer
9Outcome [Apeh]One of Active, Inactive, or Not Tested2Acylpeptide hydrolase [Mus musculus]Outcome
10P value [Apeh]The P value determined from Student's t-test for vehicle vs. compound treated samples2Float
11Spectra counts, vehicle [1, Apeh]Number of spectra counts for Apeh in vehicle treated sample; replicate [1]2Integer
12Spectra counts, vehicle [2, Apeh]Number of spectra counts for Apeh in vehicle treated sample; replicate [2]2Integer
13Spectra counts, vehicle [3, Apeh]Number of spectra counts for Apeh in vehicle treated sample; replicate [3]2Integer
14Spectra counts, test compound [1, Apeh]Number of spectra counts for Apeh in compound treated sample; replicate [1]2Integer
15Spectra counts, test compound [2, Apeh]Number of spectra counts for Apeh in compound treated sample; replicate [2]2Integer
16Spectra counts, test compound [3, Apeh]Number of spectra counts for Apeh in compound treated sample; replicate [3]2Integer
17Outcome [Ctsa]One of Active, Inactive, or Not Tested3lysosomal protective protein isoform b precursor [Mus musculus]Outcome
18P value [Ctsa]The P value determined from Student's t-test for vehicle vs. compound treated samples3Float
19Spectra counts, vehicle [1, Ctsa]Number of spectra counts for Ctsa in vehicle treated sample; replicate [1]3Integer
20Spectra counts, vehicle [2, Ctsa]Number of spectra counts for Ctsa in vehicle treated sample; replicate [2]3Integer
21Spectra counts, vehicle [3, Ctsa]Number of spectra counts for Ctsa in vehicle treated sample; replicate [3]3Integer
22Spectra counts, test compound [1, Ctsa]Number of spectra counts for Ctsa in compound treated sample; replicate [1]3Integer
23Spectra counts, test compound [2, Ctsa]Number of spectra counts for Ctsa in compound treated sample; replicate [2]3Integer
24Spectra counts, test compound [3, Ctsa]Number of spectra counts for Ctsa in compound treated sample; replicate [3]3Integer
25Outcome [Prcp]One of Active, Inactive, or Not Tested4lysosomal Pro-X carboxypeptidase precursor [Mus musculus]Outcome
26P value [Prcp]The P value determined from Student's t-test for vehicle vs. compound treated samples4Float
27Spectra counts, vehicle [1, Prcp]Number of spectra counts for Prcp in vehicle treated sample; replicate [1]4Integer
28Spectra counts, vehicle [2, Prcp]Number of spectra counts for Prcp in vehicle treated sample; replicate [2]4Integer
29Spectra counts, vehicle [3, Prcp]Number of spectra counts for Prcp in vehicle treated sample; replicate [3]4Integer
30Spectra counts, test compound [1, Prcp]Number of spectra counts for Prcp in compound treated sample; replicate [1]4Integer
31Spectra counts, test compound [2, Prcp]Number of spectra counts for Prcp in compound treated sample; replicate [2]4Integer
32Spectra counts, test compound [3, Prcp]Number of spectra counts for Prcp in compound treated sample; replicate [3]4Integer
33Outcome [Lpl]One of Active, Inactive, or Not Tested5lipoprotein lipase precursor [Mus musculus]Outcome
34P value [Lpl]The P value determined from Student's t-test for vehicle vs. compound treated samples5Float
35Spectra counts, vehicle [1, Lpl]Number of spectra counts for Lpl in vehicle treated sample; replicate [1]5Integer
36Spectra counts, vehicle [2, Lpl]Number of spectra counts for Lpl in vehicle treated sample; replicate [2]5Integer
37Spectra counts, vehicle [3, Lpl]Number of spectra counts for Lpl in vehicle treated sample; replicate [3]5Integer
38Spectra counts, test compound [1, Lpl]Number of spectra counts for Lpl in compound treated sample; replicate [1]5Integer
39Spectra counts, test compound [2, Lpl]Number of spectra counts for Lpl in compound treated sample; replicate [2]5Integer
40Spectra counts, test compound [3, Lpl]Number of spectra counts for Lpl in compound treated sample; replicate [3]5Integer
41Outcome [Lypla2]One of Active, Inactive, or Not Tested6acyl-protein thioesterase 2 [Mus musculus]Outcome
42P value [Lypla2]The P value determined from Student's t-test for vehicle vs. compound treated samples6Float
43Spectra counts, vehicle [1, Lypla2]Number of spectra counts for Lypla2 in vehicle treated sample; replicate [1]6Integer
44Spectra counts, vehicle [2, Lypla2]Number of spectra counts for Lypla2 in vehicle treated sample; replicate [2]6Integer
45Spectra counts, vehicle [3, Lypla2]Number of spectra counts for Lypla2 in vehicle treated sample; replicate [3]6Integer
46Spectra counts, test compound [1, Lypla2]Number of spectra counts for Lypla2 in compound treated sample; replicate [1]6Integer
47Spectra counts, test compound [2, Lypla2]Number of spectra counts for Lypla2 in compound treated sample; replicate [2]6Integer
48Spectra counts, test compound [3, Lypla2]Number of spectra counts for Lypla2 in compound treated sample; replicate [3]6Integer
49Outcome [Pla2g15]One of Active, Inactive, or Not Tested7group XV phospholipase A2 precursor [Mus musculus]Outcome
50P value [Pla2g15]The P value determined from Student's t-test for vehicle vs. compound treated samples7Float
51Spectra counts, vehicle [1, Pla2g15]Number of spectra counts for Pla2g15 in vehicle treated sample; replicate [1]7Integer
52Spectra counts, vehicle [2, Pla2g15]Number of spectra counts for Pla2g15 in vehicle treated sample; replicate [2]7Integer
53Spectra counts, vehicle [3, Pla2g15]Number of spectra counts for Pla2g15 in vehicle treated sample; replicate [3]7Integer
54Spectra counts, test compound [1, Pla2g15]Number of spectra counts for Pla2g15 in compound treated sample; replicate [1]7Integer
55Spectra counts, test compound [2, Pla2g15]Number of spectra counts for Pla2g15 in compound treated sample; replicate [2]7Integer
56Spectra counts, test compound [3, Pla2g15]Number of spectra counts for Pla2g15 in compound treated sample; replicate [3]7Integer
57Outcome [Ctsg]One of Active, Inactive, or Not Tested8cathepsin G preproprotein [Mus musculus]Outcome
58P value [Ctsg]The P value determined from Student's t-test for vehicle vs. compound treated samples8Float
59Spectra counts, vehicle [1, Ctsg]Number of spectra counts for Ctsg in vehicle treated sample; replicate [1]8Integer
60Spectra counts, vehicle [2, Ctsg]Number of spectra counts for Ctsg in vehicle treated sample; replicate [2]8Integer
61Spectra counts, vehicle [3, Ctsg]Number of spectra counts for Ctsg in vehicle treated sample; replicate [3]8Integer
62Spectra counts, test compound [1, Ctsg]Number of spectra counts for Ctsg in compound treated sample; replicate [1]8Integer
63Spectra counts, test compound [2, Ctsg]Number of spectra counts for Ctsg in compound treated sample; replicate [2]8Integer
64Spectra counts, test compound [3, Ctsg]Number of spectra counts for Ctsg in compound treated sample; replicate [3]8Integer
65Outcome [Elane]One of Active, Inactive, or Not Tested9neutrophil elastase precursor [Mus musculus]Outcome
66P value [Elane]The P value determined from Student's t-test for vehicle vs. compound treated samples9Float
67Spectra counts, vehicle [1, Elane]Number of spectra counts for Elane in vehicle treated sample; replicate [1]9Integer
68Spectra counts, vehicle [2, Elane]Number of spectra counts for Elane in vehicle treated sample; replicate [2]9Integer
69Spectra counts, vehicle [3, Elane]Number of spectra counts for Elane in vehicle treated sample; replicate [3]9Integer
70Spectra counts, test compound [1, Elane]Number of spectra counts for Elane in compound treated sample; replicate [1]9Integer
71Spectra counts, test compound [2, Elane]Number of spectra counts for Elane in compound treated sample; replicate [2]9Integer
72Spectra counts, test compound [3, Elane]Number of spectra counts for Elane in compound treated sample; replicate [3]9Integer
73Outcome [Dpp9]One of Active, Inactive, or Not Tested10dipeptidyl peptidase 9 [Mus musculus]Outcome
74P value [Dpp9]The P value determined from Student's t-test for vehicle vs. compound treated samples10Float
75Spectra counts, vehicle [1, Dpp9]Number of spectra counts for Dpp9 in vehicle treated sample; replicate [1]10Integer
76Spectra counts, vehicle [2, Dpp9]Number of spectra counts for Dpp9 in vehicle treated sample; replicate [2]10Integer
77Spectra counts, vehicle [3, Dpp9]Number of spectra counts for Dpp9 in vehicle treated sample; replicate [3]10Integer
78Spectra counts, test compound [1, Dpp9]Number of spectra counts for Dpp9 in compound treated sample; replicate [1]10Integer
79Spectra counts, test compound [2, Dpp9]Number of spectra counts for Dpp9 in compound treated sample; replicate [2]10Integer
80Spectra counts, test compound [3, Dpp9]Number of spectra counts for Dpp9 in compound treated sample; replicate [3]10Integer
81Outcome [Fasn]One of Active, Inactive, or Not Tested11fatty acid synthase [Mus musculus]Outcome
82P value [Fasn]The P value determined from Student's t-test for vehicle vs. compound treated samples11Float
83Spectra counts, vehicle [1, Fasn]Number of spectra counts for Fasn in vehicle treated sample; replicate [1]11Integer
84Spectra counts, vehicle [2, Fasn]Number of spectra counts for Fasn in vehicle treated sample; replicate [2]11Integer
85Spectra counts, vehicle [3, Fasn]Number of spectra counts for Fasn in vehicle treated sample; replicate [3]11Integer
86Spectra counts, test compound [1, Fasn]Number of spectra counts for Fasn in compound treated sample; replicate [1]11Integer
87Spectra counts, test compound [2, Fasn]Number of spectra counts for Fasn in compound treated sample; replicate [2]11Integer
88Spectra counts, test compound [3, Fasn]Number of spectra counts for Fasn in compound treated sample; replicate [3]11Integer
89Outcome [Acot2]One of Active, Inactive, or Not Tested12acyl-coenzyme A thioesterase 2, mitochondrial precursor [Mus musculus]Outcome
90P value [Acot2]The P value determined from Student's t-test for vehicle vs. compound treated samples12Float
91Spectra counts, vehicle [1, Acot2]Number of spectra counts for Acot2 in vehicle treated sample; replicate [1]12Integer
92Spectra counts, vehicle [2, Acot2]Number of spectra counts for Acot2 in vehicle treated sample; replicate [2]12Integer
93Spectra counts, vehicle [3, Acot2]Number of spectra counts for Acot2 in vehicle treated sample; replicate [3]12Integer
94Spectra counts, test compound [1, Acot2]Number of spectra counts for Acot2 in compound treated sample; replicate [1]12Integer
95Spectra counts, test compound [2, Acot2]Number of spectra counts for Acot2 in compound treated sample; replicate [2]12Integer
96Spectra counts, test compound [3, Acot2]Number of spectra counts for Acot2 in compound treated sample; replicate [3]12Integer
97Outcome [Lypla1]One of Active, Inactive, or Not Tested13acyl-protein thioesterase 1 [Mus musculus]Outcome
98P value [Lypla1]The P value determined from Student's t-test for vehicle vs. compound treated samples13Float
99Spectra counts, vehicle [1, Lypla1]Number of spectra counts for Lypla1 in vehicle treated sample; replicate [1]13Integer
100Spectra counts, vehicle [2, Lypla1]Number of spectra counts for Lypla1 in vehicle treated sample; replicate [2]13Integer
101Spectra counts, vehicle [3, Lypla1]Number of spectra counts for Lypla1 in vehicle treated sample; replicate [3]13Integer
102Spectra counts, test compound [1, Lypla1]Number of spectra counts for Lypla1 in compound treated sample; replicate [1]13Integer
103Spectra counts, test compound [2, Lypla1]Number of spectra counts for Lypla1 in compound treated sample; replicate [2]13Integer
104Spectra counts, test compound [3, Lypla1]Number of spectra counts for Lypla1 in compound treated sample; replicate [3]13Integer
105Outcome [Dpp8]One of Active, Inactive, or Not Tested14dipeptidyl peptidase 8 [Mus musculus]Outcome
106P value [Dpp8]The P value determined from Student's t-test for vehicle vs. compound treated samples14Float
107Spectra counts, vehicle [1, Dpp8]Number of spectra counts for Dpp8 in vehicle treated sample; replicate [1]14Integer
108Spectra counts, vehicle [2, Dpp8]Number of spectra counts for Dpp8 in vehicle treated sample; replicate [2]14Integer
109Spectra counts, vehicle [3, Dpp8]Number of spectra counts for Dpp8 in vehicle treated sample; replicate [3]14Integer
110Spectra counts, test compound [1, Dpp8]Number of spectra counts for Dpp8 in compound treated sample; replicate [1]14Integer
111Spectra counts, test compound [2, Dpp8]Number of spectra counts for Dpp8 in compound treated sample; replicate [2]14Integer
112Spectra counts, test compound [3, Dpp8]Number of spectra counts for Dpp8 in compound treated sample; replicate [3]14Integer
113Outcome [Acot1]One of Active, Inactive, or Not Tested15acyl-coenzyme A thioesterase 1 [Mus musculus]Outcome
114P value [Acot1]The P value determined from Student's t-test for vehicle vs. compound treated samples15Float
115Spectra counts, vehicle [1, Acot1]Number of spectra counts for Acot1 in vehicle treated sample; replicate [1]15Integer
116Spectra counts, vehicle [2, Acot1]Number of spectra counts for Acot1 in vehicle treated sample; replicate [2]15Integer
117Spectra counts, vehicle [3, Acot1]Number of spectra counts for Acot1 in vehicle treated sample; replicate [3]15Integer
118Spectra counts, test compound [1, Acot1]Number of spectra counts for Acot1 in compound treated sample; replicate [1]15Integer
119Spectra counts, test compound [2, Acot1]Number of spectra counts for Acot1 in compound treated sample; replicate [2]15Integer
120Spectra counts, test compound [3, Acot1]Number of spectra counts for Acot1 in compound treated sample; replicate [3]15Integer
121Outcome [Pnpla7]One of Active, Inactive, or Not Tested16patatin-like phospholipase domain-containing protein 7 [Mus musculus]Outcome
122P value [Pnpla7]The P value determined from Student's t-test for vehicle vs. compound treated samples16Float
123Spectra counts, vehicle [1, Pnpla7]Number of spectra counts for Pnpla7 in vehicle treated sample; replicate [1]16Integer
124Spectra counts, vehicle [2, Pnpla7]Number of spectra counts for Pnpla7 in vehicle treated sample; replicate [2]16Integer
125Spectra counts, vehicle [3, Pnpla7]Number of spectra counts for Pnpla7 in vehicle treated sample; replicate [3]16Integer
126Spectra counts, test compound [1, Pnpla7]Number of spectra counts for Pnpla7 in compound treated sample; replicate [1]16Integer
127Spectra counts, test compound [2, Pnpla7]Number of spectra counts for Pnpla7 in compound treated sample; replicate [2]16Integer
128Spectra counts, test compound [3, Pnpla7]Number of spectra counts for Pnpla7 in compound treated sample; replicate [3]16Integer
129Outcome [Dpp4]One of Active, Inactive, or Not Tested17dipeptidyl peptidase 4 isoform 1 [Mus musculus]Outcome
130P value [Dpp4]The P value determined from Student's t-test for vehicle vs. compound treated samples17Float
131Spectra counts, vehicle [1, Dpp4]Number of spectra counts for Dpp4 in vehicle treated sample; replicate [1]17Integer
132Spectra counts, vehicle [2, Dpp4]Number of spectra counts for Dpp4 in vehicle treated sample; replicate [2]17Integer
133Spectra counts, vehicle [3, Dpp4]Number of spectra counts for Dpp4 in vehicle treated sample; replicate [3]17Integer
134Spectra counts, test compound [1, Dpp4]Number of spectra counts for Dpp4 in compound treated sample; replicate [1]17Integer
135Spectra counts, test compound [2, Dpp4]Number of spectra counts for Dpp4 in compound treated sample; replicate [2]17Integer
136Spectra counts, test compound [3, Dpp4]Number of spectra counts for Dpp4 in compound treated sample; replicate [3]17Integer
137Outcome [Lipe]One of Active, Inactive, or Not Tested18hormone-sensitive lipase isoform 2 [Mus musculus]Outcome
138P value [Lipe]The P value determined from Student's t-test for vehicle vs. compound treated samples18Float
139Spectra counts, vehicle [1, Lipe]Number of spectra counts for Lipe in vehicle treated sample; replicate [1]18Integer
140Spectra counts, vehicle [2, Lipe]Number of spectra counts for Lipe in vehicle treated sample; replicate [2]18Integer
141Spectra counts, vehicle [3, Lipe]Number of spectra counts for Lipe in vehicle treated sample; replicate [3]18Integer
142Spectra counts, test compound [1, Lipe]Number of spectra counts for Lipe in compound treated sample; replicate [1]18Integer
143Spectra counts, test compound [2, Lipe]Number of spectra counts for Lipe in compound treated sample; replicate [2]18Integer
144Spectra counts, test compound [3, Lipe]Number of spectra counts for Lipe in compound treated sample; replicate [3]18Integer
145Outcome [Abhd12]One of Active, Inactive, or Not Tested19monoacylglycerol lipase ABHD12 [Mus musculus]Outcome
146P value [Abhd12]The P value determined from Student's t-test for vehicle vs. compound treated samples19Float
147Spectra counts, vehicle [1, Abhd12]Number of spectra counts for Abhd12 in vehicle treated sample; replicate [1]19Integer
148Spectra counts, vehicle [2, Abhd12]Number of spectra counts for Abhd12 in vehicle treated sample; replicate [2]19Integer
149Spectra counts, vehicle [3, Abhd12]Number of spectra counts for Abhd12 in vehicle treated sample; replicate [3]19Integer
150Spectra counts, test compound [1, Abhd12]Number of spectra counts for Abhd12 in compound treated sample; replicate [1]19Integer
151Spectra counts, test compound [2, Abhd12]Number of spectra counts for Abhd12 in compound treated sample; replicate [2]19Integer
152Spectra counts, test compound [3, Abhd12]Number of spectra counts for Abhd12 in compound treated sample; replicate [3]19Integer
153Outcome [Pla2g7]One of Active, Inactive, or Not Tested20platelet-activating factor acetylhydrolase precursor [Mus musculus]Outcome
154P value [Pla2g7]The P value determined from Student's t-test for vehicle vs. compound treated samples20Float
155Spectra counts, vehicle [1, Pla2g7]Number of spectra counts for Pla2g7 in vehicle treated sample; replicate [1]20Integer
156Spectra counts, vehicle [2, Pla2g7]Number of spectra counts for Pla2g7 in vehicle treated sample; replicate [2]20Integer
157Spectra counts, vehicle [3, Pla2g7]Number of spectra counts for Pla2g7 in vehicle treated sample; replicate [3]20Integer
158Spectra counts, test compound [1, Pla2g7]Number of spectra counts for Pla2g7 in compound treated sample; replicate [1]20Integer
159Spectra counts, test compound [2, Pla2g7]Number of spectra counts for Pla2g7 in compound treated sample; replicate [2]20Integer
160Spectra counts, test compound [3, Pla2g7]Number of spectra counts for Pla2g7 in compound treated sample; replicate [3]20Integer
161Outcome [Pafah1b2]One of Active, Inactive, or Not Tested21platelet-activating factor acetylhydrolase IB subunit beta [Mus musculus]Outcome
162P value [Pafah1b2]The P value determined from Student's t-test for vehicle vs. compound treated samples21Float
163Spectra counts, vehicle [1, Pafah1b2]Number of spectra counts for Pafah1b2 in vehicle treated sample; replicate [1]21Integer
164Spectra counts, vehicle [2, Pafah1b2]Number of spectra counts for Pafah1b2 in vehicle treated sample; replicate [2]21Integer
165Spectra counts, vehicle [3, Pafah1b2]Number of spectra counts for Pafah1b2 in vehicle treated sample; replicate [3]21Integer
166Spectra counts, test compound [1, Pafah1b2]Number of spectra counts for Pafah1b2 in compound treated sample; replicate [1]21Integer
167Spectra counts, test compound [2, Pafah1b2]Number of spectra counts for Pafah1b2 in compound treated sample; replicate [2]21Integer
168Spectra counts, test compound [3, Pafah1b2]Number of spectra counts for Pafah1b2 in compound treated sample; replicate [3]21Integer
169Outcome [Pafah2]One of Active, Inactive, or Not Tested22platelet-activating factor acetylhydrolase 2, cytoplasmic [Mus musculus]Outcome
170P value [Pafah2]The P value determined from Student's t-test for vehicle vs. compound treated samples22Float
171Spectra counts, vehicle [1, Pafah2]Number of spectra counts for Pafah2 in vehicle treated sample; replicate [1]22Integer
172Spectra counts, vehicle [2, Pafah2]Number of spectra counts for Pafah2 in vehicle treated sample; replicate [2]22Integer
173Spectra counts, vehicle [3, Pafah2]Number of spectra counts for Pafah2 in vehicle treated sample; replicate [3]22Integer
174Spectra counts, test compound [1, Pafah2]Number of spectra counts for Pafah2 in compound treated sample; replicate [1]22Integer
175Spectra counts, test compound [2, Pafah2]Number of spectra counts for Pafah2 in compound treated sample; replicate [2]22Integer
176Spectra counts, test compound [3, Pafah2]Number of spectra counts for Pafah2 in compound treated sample; replicate [3]22Integer
177Outcome [Magl]One of Active, Inactive, or Not Tested23monoglyceride lipase isoform d [Mus musculus]Outcome
178P value [Magl]The P value determined from Student's t-test for vehicle vs. compound treated samples23Float
179Spectra counts, vehicle [1, Magl]Number of spectra counts for Magl in vehicle treated sample; replicate [1]23Integer
180Spectra counts, vehicle [2, Magl]Number of spectra counts for Magl in vehicle treated sample; replicate [2]23Integer
181Spectra counts, vehicle [3, Magl]Number of spectra counts for Magl in vehicle treated sample; replicate [3]23Integer
182Spectra counts, test compound [1, Magl]Number of spectra counts for Magl in compound treated sample; replicate [1]23Integer
183Spectra counts, test compound [2, Magl]Number of spectra counts for Magl in compound treated sample; replicate [2]23Integer
184Spectra counts, test compound [3, Magl]Number of spectra counts for Magl in compound treated sample; replicate [3]23Integer
185Outcome [Daglb]One of Active, Inactive, or Not Tested24sn1-specific diacylglycerol lipase beta [Mus musculus]Outcome
186P value [Daglb]The P value determined from Student's t-test for vehicle vs. compound treated samples24Float
187Spectra counts, vehicle [1, Daglb]Number of spectra counts for Daglb in vehicle treated sample; replicate [1]24Integer
188Spectra counts, vehicle [2, Daglb]Number of spectra counts for Daglb in vehicle treated sample; replicate [2]24Integer
189Spectra counts, vehicle [3, Daglb]Number of spectra counts for Daglb in vehicle treated sample; replicate [3]24Integer
190Spectra counts, test compound [1, Daglb]Number of spectra counts for Daglb in compound treated sample; replicate [1]24Integer
191Spectra counts, test compound [2, Daglb]Number of spectra counts for Daglb in compound treated sample; replicate [2]24Integer
192Spectra counts, test compound [3, Daglb]Number of spectra counts for Daglb in compound treated sample; replicate [3]24Integer
193Outcome [Pafah1b3]One of Active, Inactive, or Not Tested25platelet-activating factor acetylhydrolase IB subunit gamma [Mus musculus]Outcome
194P value [Pafah1b3]The P value determined from Student's t-test for vehicle vs. compound treated samples25Float
195Spectra counts, vehicle [1, Pafah1b3]Number of spectra counts for Pafah1b3 in vehicle treated sample; replicate [1]25Integer
196Spectra counts, vehicle [2, Pafah1b3]Number of spectra counts for Pafah1b3 in vehicle treated sample; replicate [2]25Integer
197Spectra counts, vehicle [3, Pafah1b3]Number of spectra counts for Pafah1b3 in vehicle treated sample; replicate [3]25Integer
198Spectra counts, test compound [1, Pafah1b3]Number of spectra counts for Pafah1b3 in compound treated sample; replicate [1]25Integer
199Spectra counts, test compound [2, Pafah1b3]Number of spectra counts for Pafah1b3 in compound treated sample; replicate [2]25Integer
200Spectra counts, test compound [3, Pafah1b3]Number of spectra counts for Pafah1b3 in compound treated sample; replicate [3]25Integer
201Outcome [Dpp7]One of Active, Inactive, or Not Tested26dipeptidyl peptidase 2 precursor [Mus musculus]Outcome
202P value [Dpp7]The P value determined from Student's t-test for vehicle vs. compound treated samples26Float
203Spectra counts, vehicle [1, Dpp7]Number of spectra counts for Dpp7 in vehicle treated sample; replicate [1]26Integer
204Spectra counts, vehicle [2, Dpp7]Number of spectra counts for Dpp7 in vehicle treated sample; replicate [2]26Integer
205Spectra counts, vehicle [3, Dpp7]Number of spectra counts for Dpp7 in vehicle treated sample; replicate [3]26Integer
206Spectra counts, test compound [1, Dpp7]Number of spectra counts for Dpp7 in compound treated sample; replicate [1]26Integer
207Spectra counts, test compound [2, Dpp7]Number of spectra counts for Dpp7 in compound treated sample; replicate [2]26Integer
208Spectra counts, test compound [3, Dpp7]Number of spectra counts for Dpp7 in compound treated sample; replicate [3]26Integer
209Outcome [Abhd10]One of Active, Inactive, or Not Tested27abhydrolase domain-containing protein 10, mitochondrial precursor [Mus musculus]Outcome
210P value [Abhd10]The P value determined from Student's t-test for vehicle vs. compound treated samples27Float
211Spectra counts, vehicle [1, Abhd10]Number of spectra counts for Abhd10 in vehicle treated sample; replicate [1]27Integer
212Spectra counts, vehicle [2, Abhd10]Number of spectra counts for Abhd10 in vehicle treated sample; replicate [2]27Integer
213Spectra counts, vehicle [3, Abhd10]Number of spectra counts for Abhd10 in vehicle treated sample; replicate [3]27Integer
214Spectra counts, test compound [1, Abhd10]Number of spectra counts for Abhd10 in compound treated sample; replicate [1]27Integer
215Spectra counts, test compound [2, Abhd10]Number of spectra counts for Abhd10 in compound treated sample; replicate [2]27Integer
216Spectra counts, test compound [3, Abhd10]Number of spectra counts for Abhd10 in compound treated sample; replicate [3]27Integer
217Outcome [Aoah]One of Active, Inactive, or Not Tested28acyloxyacyl hydrolase precursor [Mus musculus]Outcome
218P value [Aoah]The P value determined from Student's t-test for vehicle vs. compound treated samples28Float
219Spectra counts, vehicle [1, Aoah]Number of spectra counts for Aoah in vehicle treated sample; replicate [1]28Integer
220Spectra counts, vehicle [2, Aoah]Number of spectra counts for Aoah in vehicle treated sample; replicate [2]28Integer
221Spectra counts, vehicle [3, Aoah]Number of spectra counts for Aoah in vehicle treated sample; replicate [3]28Integer
222Spectra counts, test compound [1, Aoah]Number of spectra counts for Aoah in compound treated sample; replicate [1]28Integer
223Spectra counts, test compound [2, Aoah]Number of spectra counts for Aoah in compound treated sample; replicate [2]28Integer
224Spectra counts, test compound [3, Aoah]Number of spectra counts for Aoah in compound treated sample; replicate [3]28Integer
225Outcome [Pnpla6]One of Active, Inactive, or Not Tested29neuropathy target esterase isoform 1 [Mus musculus]Outcome
226P value [Pnpla6]The P value determined from Student's t-test for vehicle vs. compound treated samples29Float
227Spectra counts, vehicle [1, Pnpla6]Number of spectra counts for Pnpla6 in vehicle treated sample; replicate [1]29Integer
228Spectra counts, vehicle [2, Pnpla6]Number of spectra counts for Pnpla6 in vehicle treated sample; replicate [2]29Integer
229Spectra counts, vehicle [3, Pnpla6]Number of spectra counts for Pnpla6 in vehicle treated sample; replicate [3]29Integer
230Spectra counts, test compound [1, Pnpla6]Number of spectra counts for Pnpla6 in compound treated sample; replicate [1]29Integer
231Spectra counts, test compound [2, Pnpla6]Number of spectra counts for Pnpla6 in compound treated sample; replicate [2]29Integer
232Spectra counts, test compound [3, Pnpla6]Number of spectra counts for Pnpla6 in compound treated sample; replicate [3]29Integer
233Outcome [Abhd11]One of Active, Inactive, or Not Tested30abhydrolase domain-containing protein 11 isoform 1 [Mus musculus]Outcome
234P value [Abhd11]The P value determined from Student's t-test for vehicle vs. compound treated samples30Float
235Spectra counts, vehicle [1, Abhd11]Number of spectra counts for Abhd11 in vehicle treated sample; replicate [1]30Integer
236Spectra counts, vehicle [2, Abhd11]Number of spectra counts for Abhd11 in vehicle treated sample; replicate [2]30Integer
237Spectra counts, vehicle [3, Abhd11]Number of spectra counts for Abhd11 in vehicle treated sample; replicate [3]30Integer
238Spectra counts, test compound [1, Abhd11]Number of spectra counts for Abhd11 in compound treated sample; replicate [1]30Integer
239Spectra counts, test compound [2, Abhd11]Number of spectra counts for Abhd11 in compound treated sample; replicate [2]30Integer
240Spectra counts, test compound [3, Abhd11]Number of spectra counts for Abhd11 in compound treated sample; replicate [3]30Integer
241Outcome [Tpp2]One of Active, Inactive, or Not Tested31Tpp2 protein [Mus musculus]Outcome
242P value [Tpp2]The P value determined from Student's t-test for vehicle vs. compound treated samples31Float
243Spectra counts, vehicle [1, Tpp2]Number of spectra counts for Tpp2 in vehicle treated sample; replicate [1]31Integer
244Spectra counts, vehicle [2, Tpp2]Number of spectra counts for Tpp2 in vehicle treated sample; replicate [2]31Integer
245Spectra counts, vehicle [3, Tpp2]Number of spectra counts for Tpp2 in vehicle treated sample; replicate [3]31Integer
246Spectra counts, test compound [1, Tpp2]Number of spectra counts for Tpp2 in compound treated sample; replicate [1]31Integer
247Spectra counts, test compound [2, Tpp2]Number of spectra counts for Tpp2 in compound treated sample; replicate [2]31Integer
248Spectra counts, test compound [3, Tpp2]Number of spectra counts for Tpp2 in compound treated sample; replicate [3]31Integer
249Outcome [Fam108b]One of Active, Inactive, or Not Tested32abhydrolase domain-containing protein FAM108B1 precursor [Mus musculus]Outcome
250P value [Fam108b]The P value determined from Student's t-test for vehicle vs. compound treated samples32Float
251Spectra counts, vehicle [1, Fam108b]Number of spectra counts for Fam108b in vehicle treated sample; replicate [1]32Integer
252Spectra counts, vehicle [2, Fam108b]Number of spectra counts for Fam108b in vehicle treated sample; replicate [2]32Integer
253Spectra counts, vehicle [3, Fam108b]Number of spectra counts for Fam108b in vehicle treated sample; replicate [3]32Integer
254Spectra counts, test compound [1, Fam108b]Number of spectra counts for Fam108b in compound treated sample; replicate [1]32Integer
255Spectra counts, test compound [2, Fam108b]Number of spectra counts for Fam108b in compound treated sample; replicate [2]32Integer
256Spectra counts, test compound [3, Fam108b]Number of spectra counts for Fam108b in compound treated sample; replicate [3]32Integer
257Outcome [Iah1]One of Active, Inactive, or Not Tested33isoamyl acetate-hydrolyzing esterase 1 homolog [Mus musculus]Outcome
258P value [Iah1]The P value determined from Student's t-test for vehicle vs. compound treated samples33Float
259Spectra counts, vehicle [1, Iah1]Number of spectra counts for Iah1 in vehicle treated sample; replicate [1]33Integer
260Spectra counts, vehicle [2, Iah1]Number of spectra counts for Iah1 in vehicle treated sample; replicate [2]33Integer
261Spectra counts, vehicle [3, Iah1]Number of spectra counts for Iah1 in vehicle treated sample; replicate [3]33Integer
262Spectra counts, test compound [1, Iah1]Number of spectra counts for Iah1 in compound treated sample; replicate [1]33Integer
263Spectra counts, test compound [2, Iah1]Number of spectra counts for Iah1 in compound treated sample; replicate [2]33Integer
264Spectra counts, test compound [3, Iah1]Number of spectra counts for Iah1 in compound treated sample; replicate [3]33Integer
265Outcome [Prepl]One of Active, Inactive, or Not Tested34prolyl endopeptidase-like isoform a [Mus musculus]Outcome
266P value [Prepl]The P value determined from Student's t-test for vehicle vs. compound treated samples34Float
267Spectra counts, vehicle [1, Prepl]Number of spectra counts for Prepl in vehicle treated sample; replicate [1]34Integer
268Spectra counts, vehicle [2, Prepl]Number of spectra counts for Prepl in vehicle treated sample; replicate [2]34Integer
269Spectra counts, vehicle [3, Prepl]Number of spectra counts for Prepl in vehicle treated sample; replicate [3]34Integer
270Spectra counts, test compound [1, Prepl]Number of spectra counts for Prepl in compound treated sample; replicate [1]34Integer
271Spectra counts, test compound [2, Prepl]Number of spectra counts for Prepl in compound treated sample; replicate [2]34Integer
272Spectra counts, test compound [3, Prepl]Number of spectra counts for Prepl in compound treated sample; replicate [3]34Integer
273Outcome [Abhd6]One of Active, Inactive, or Not Tested35monoacylglycerol lipase ABHD6 [Mus musculus]Outcome
274P value [Abhd6]The P value determined from Student's t-test for vehicle vs. compound treated samples35Float
275Spectra counts, vehicle [1, Abhd6]Number of spectra counts for Abhd6 in vehicle treated sample; replicate [1]35Integer
276Spectra counts, vehicle [2, Abhd6]Number of spectra counts for Abhd6 in vehicle treated sample; replicate [2]35Integer
277Spectra counts, vehicle [3, Abhd6]Number of spectra counts for Abhd6 in vehicle treated sample; replicate [3]35Integer
278Spectra counts, test compound [1, Abhd6]Number of spectra counts for Abhd6 in compound treated sample; replicate [1]35Integer
279Spectra counts, test compound [2, Abhd6]Number of spectra counts for Abhd6 in compound treated sample; replicate [2]35Integer
280Spectra counts, test compound [3, Abhd6]Number of spectra counts for Abhd6 in compound treated sample; replicate [3]35Integer
281Outcome [Ppt2]One of Active, Inactive, or Not Tested36lysosomal thioesterase PPT2 precursor [Mus musculus]Outcome
282P value [Ppt2]The P value determined from Student's t-test for vehicle vs. compound treated samples36Float
283Spectra counts, vehicle [1, Ppt2]Number of spectra counts for Ppt2 in vehicle treated sample; replicate [1]36Integer
284Spectra counts, vehicle [2, Ppt2]Number of spectra counts for Ppt2 in vehicle treated sample; replicate [2]36Integer
285Spectra counts, vehicle [3, Ppt2]Number of spectra counts for Ppt2 in vehicle treated sample; replicate [3]36Integer
286Spectra counts, test compound [1, Ppt2]Number of spectra counts for Ppt2 in compound treated sample; replicate [1]36Integer
287Spectra counts, test compound [2, Ppt2]Number of spectra counts for Ppt2 in compound treated sample; replicate [2]36Integer
288Spectra counts, test compound [3, Ppt2]Number of spectra counts for Ppt2 in compound treated sample; replicate [3]36Integer
289Outcome [Gzma]One of Active, Inactive, or Not Tested37granzyme A precursor [Mus musculus]Outcome
290P value [Gzma]The P value determined from Student's t-test for vehicle vs. compound treated samples37Float
291Spectra counts, vehicle [1, Gzma]Number of spectra counts for Gzma in vehicle treated sample; replicate [1]37Integer
292Spectra counts, vehicle [2, Gzma]Number of spectra counts for Gzma in vehicle treated sample; replicate [2]37Integer
293Spectra counts, vehicle [3, Gzma]Number of spectra counts for Gzma in vehicle treated sample; replicate [3]37Integer
294Spectra counts, test compound [1, Gzma]Number of spectra counts for Gzma in compound treated sample; replicate [1]37Integer
295Spectra counts, test compound [2, Gzma]Number of spectra counts for Gzma in compound treated sample; replicate [2]37Integer
296Spectra counts, test compound [3, Gzma]Number of spectra counts for Gzma in compound treated sample; replicate [3]37Integer
297Outcome [Lipa]One of Active, Inactive, or Not Tested38lysosomal acid lipase/cholesteryl ester hydrolase precursor [Mus musculus]Outcome
298P value [Lipa]The P value determined from Student's t-test for vehicle vs. compound treated samples38Float
299Spectra counts, vehicle [1, Lipa]Number of spectra counts for Lipa in vehicle treated sample; replicate [1]38Integer
300Spectra counts, vehicle [2, Lipa]Number of spectra counts for Lipa in vehicle treated sample; replicate [2]38Integer
301Spectra counts, vehicle [3, Lipa]Number of spectra counts for Lipa in vehicle treated sample; replicate [3]38Integer
302Spectra counts, test compound [1, Lipa]Number of spectra counts for Lipa in compound treated sample; replicate [1]38Integer
303Spectra counts, test compound [2, Lipa]Number of spectra counts for Lipa in compound treated sample; replicate [2]38Integer
304Spectra counts, test compound [3, Lipa]Number of spectra counts for Lipa in compound treated sample; replicate [3]38Integer
Additional Information
Grant Number: 1 R01 DA025285

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