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BioAssay: AID 588787

Late stage assay provider results from the probe development effort to identify inhibitors of pPAFAH: LC-MS/MS-based biochemical ABPP-MudPIT assay to determine selectivity of test compounds in the mouse brain soluble proteome

Name: Late stage assay provider results from the probe development effort to identify inhibitors of pPAFAH: LC-MS/MS-based biochemical ABPP-MudPIT assay to determine selectivity of test compounds in the mouse brain soluble proteome. ..more
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AID: 588787
Data Source: The Scripps Research Institute Molecular Screening Center (pPAFAH_INH_LCMS_SOL)
BioAssay Type: Panel
Depositor Category: NIH Molecular Libraries Probe Production Network, Assay Provider
BioAssay Version:
Deposit Date: 2011-11-12
Hold-until Date: 2012-05-31
Modify Date: 2012-05-31

Data Table ( Complete ):           Active    All
BioActive Compound: 1
Depositor Specified Assays
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AIDNameTypeProbeComment
463082Fluorescence polarization-based primary biochemical high throughput screening assay to identify inhibitors of the plasma platelet activating factor acetylhydrolase (pPAFAH)screening Primary screen (pPAFAH inhibitors in singlicate)
463092Summary of probe development efforts to identify inhibitors of the plasma platelet activating factor acetylhydrolase (pPAFAH)summary1 Summary (pPAFAH inhibitors)
463230Fluorescence polarization-based biochemical high throughput confirmation assay for inhibitors of the plasma platelet activating factor acetylhydrolase (pPAFAH; PLA2G7)screening Confirmation screen (pPAFAH inhibitors in triplicate)
588474Late stage assay provider results from the probe development effort to identify inhibitors of plasma platelet activating factor acetylhydrolase (pPAFAH): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of pPAFAHother ABPP Screen (pPAFAH inhibitors in singlicate)
588770Late stage assay provider results from the probe development effort to identify inhibitors of pPAFAH: fluorescence-based dose-response biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) assay for SAR compoundsconfirmatory ABBP Screen (3XIC50; pPAFAH INH; "SAR compounds)
588769Late stage assay provider results from the probe development effort to identify inhibitors of plasma platelet activating factor acetylhydrolase (pPAFAH): fluorescence-based dose response biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) assay for HTS compoundsconfirmatory ABPP Screen (3XIC50; pPAFAH INH; HTS compounds)
588774Late stage assay provider results from the probe development effort to identify inhibitors of plasma platelet activating factor acetylhydrolase (pPAFAH): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition and selectivity of SAR compounds in a complex proteomeother ABPP Screen (Inhibition and selectivity; SAR compounds)
588766Late stage assay provider results from the probe development effort to identify inhibitors of plasma platelet activating factor acetylhydrolase (pPAFAH): fluorescence-based dose response biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of human pPAFAHconfirmatory Cytox assay (5XCC50; 293T cells)
588773Late stage assay provider results from the probe development effort to identify inhibitors of plasma platelet activating factor acetylhydrolase (pPAFAH): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition and selectivity of HTS compounds in a complex proteomeother ABPP Screen (Inhibition and selectivity; HTS compounds)
588767Late stage assay provider results from the probe development effort to identify inhibitors of plasma platelet activating factor acetylhydrolase (pPAFAH): fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of PAFAH2other ABPP Screen (PAFAH2 Counterscreen)
588817Late stage assay provider results from the probe development effort to identify inhibitors of pPAFAH: fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of pPAFAH in situother
588823Late stage assay provider results from the probe development effort to identify inhibitors of pPAFAH: fluorescence-based biochemical gel-based competitive Activity-Based Protein Profiling (ABPP) inhibition of pPAFAH and selectivity analysis in vivoother
Description:
Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC)
Center Affiliation: The Scripps Research Institute (TSRI)
Assay Providers: Brian Bahnson (Univ. of Delaware); Benjamin Cravatt, (TSRI)
Network: Molecular Libraries Probe Production Centers Network (MLPCN)
Grant Proposal Number: 1R01HL084366
Grant Proposal PI: Brian Bahnson
External Assay ID: pPAFAH_INH_LCMS_SOL

Name: Late stage assay provider results from the probe development effort to identify inhibitors of pPAFAH: LC-MS/MS-based biochemical ABPP-MudPIT assay to determine selectivity of test compounds in the mouse brain soluble proteome.

Description:

This project aims to develop specific inhibitors of plasma platelet activating factor acetylhydrolase (pPAFAH), and three associated members of the serine hydrolase family of enzymes-PAFAH2, PAFAH1b2, and PAFAH1b3. pPAFAH, an enzyme linked to the inflammatory pathways involved in atherosclerosis, asthma, anaphylactic shock, and other allergic reactions (1,2), is a lipoprotein-associated group VIIA phospholipase A2 that reduces the levels of the signaling molecule platelet activating factor (PAF) (3,4), a potent pro-inflammatory phospholipid signaling molecule (5), and other pro-inflammatory agents, such as oxidized phospholipids, through hydrolysis. A large number of studies have been published over the years since pPAFAH was first discovered linking an increase in pPAFAH concentration and/or activity to an increased risk of various cardiovascular diseases (6,7). The biological function of pPAFAH in the development of coronary heart diseases (CHD) is controversial, with both anti- and pro-inflammatory roles attributed to it (8,9). Dr. Bahnson and colleagues recently reported the first high-resolution crystal structure of the pPAFAH enzyme (10), and would like to expand their studies to co-crystallize pPAFAH with substrate-mimetic inhibitors to further define the active site and substrate specificity of pPAFAH. While one selective pPAFAH inhibitor has been reported (11), its properties are not suitable for the proposed studies. Given the complex biology of the pPAFAH enzymes, a complete characterization of their patho/physiological roles in lipid metabolism is necessary to maximize the success of therapeutic intervention. Towards this goal, development of selective inhibitors would significantly advance our understanding of these enzymes' substrate specificity and contribution to inflammatory disease processes including atherosclerosis, asthma, and rheumatoid arthritis. Pan-PAFAH inhibitors might be of heightened therapeutic value.

References:

1. Karasawa, K., Harada, A., Satoh, N., Inoue, K., and Setaka, M. (2003) Plasma platelet activating factor-acetylhydrolase (PAF-AH), Prog Lipid Res 42, 93-114.
2. Leitinger, N. (2005) Oxidized phospholipids as triggers of inflammation in atherosclerosis, Molecular Nutrition & Food Research 49, 1063-1071.
3. Blank, M. L., Lee, T., Fitzgerald, V., and Snyder, F. (1981) A specific acetylhydrolase for 1-alkyl-2- acetyl-sn-glycero-3-phosphocholine (a hypotensive and platelet-activating lipid), J Biol Chem 256, 175-178.
4. Farr, R. S., Cox, C. P., Wardlow, M. L., and Jorgensen, R. (1980) Preliminary studies of an acid labile factor (ALF) in human sera that inactivates platelet-activating factor (PAF), Clin Immunol Immunopathol 15, 318-330.
5. Zimmerman, G. A., McIntyre, T. M., Prescott, S. M., and Stafforini, D. M. (2002) The plateletactivating factor signaling system and its regulators in syndromes of inflammation and thrombosis, Crit Care Med 30, S294-301.
6. Anderson, J. L. (2008) Lipoprotein-associated phospholipase A2: an independent predictor of coronary artery disease events in primary and secondary prevention, Am J Cardiol 101, 23F-33F.
7. Sudhir, K. (2005) Clinical review: Lipoprotein-associated phospholipase A2, a novel inflammatory biomarker and independent risk predictor for cardiovascular disease, J Clin Endocrinol Metab 90, 3100-3105.
8. Wilensky, R. L., and Macphee, C. H. (2009) Lipoprotein-associated phospholipase A(2) and atherosclerosis, Curr Opin Lipidol 20, 415-420.
9. Karabina, S. A., and Ninio, E. (2006) Plasma PAF-acetylhydrolase: an unfulfilled promise?, Biochim Biophys Acta 1761, 1351-1358.
10. Samanta, U., and Bahnson, B. J. (2008) Crystal structure of human plasma platelet-activating factor acetylhydrolase: structural implication to lipoprotein binding and catalysis, J Biol Chem 283, 31617-31624.
11. Blackie, J. A., Bloomer, J. C., Brown, M. J. B., Cheng, H. Y., Hammond, B., Hickey, D. M. B., Ife, R. J., Leach, C. A., Lewis, V. A., Macphee, C. H., Milliner, K. J., Moores, K. E., Pinto, I. L., Smith, S. A., Stansfield, I. G., Stanway, S. J., Taylor, M. A., and Theobald, C. J. (2003) The identification of clinical candidate SB-480848: A potent inhibitor of lipoprotein-associated phospholipase A(2), Bioorganic & Medicinal Chemistry Letters 13, 1067-1070.

Keywords:

late stage, late stage AID, powders, assay provider, low throughput, secondary, PLA2G7, pPAFAH, serine hydrolase, platelet activating factor acetylhydrolase, inflammation, atherosclerosis, fluorescence, competitive activity-based protein profiling, ABPP, liquid chromatography, LC, tandem mass spectrometry, MS/MS, multidimensional protein identification technology, MudPIT, ABPP-MudPIT, inhibitor, selectivity, Scripps, The Scripps Research Institute Molecular Screening Center, SRIMSC, Molecular Libraries Probe Production Centers Network, MLPCN
Panel Information
Targets
    Data Table(Active)    Data Table(All)Show more
PID§NameSubstancePanel TargetsDescriptionAdditional Information
ActiveInactive
1Pla2g7 (pPAFAH)1platelet-activating factor acetylhydrolase [Mus musculus] [gi:31980752]
Taxonomy id: 10090
Gene id: 27226
2Esd1S-formylglutathione hydrolase [Mus musculus] [gi:13937355]
Taxonomy id: 10090
Gene id: 13885
3Prep1prolyl endopeptidase [Mus musculus] [gi:6755152]
Taxonomy id: 10090
Gene id: 19072
4Prepl1prolyl endopeptidase-like isoform a [Mus musculus] [gi:254939518]
Taxonomy id: 10090
Gene id: 213760
5Lypla21acyl-protein thioesterase 2 [Mus musculus] [gi:7242156]
Taxonomy id: 10090
Gene id: 26394
6Fasn1fatty acid synthase [Mus musculus] [gi:93102409]
Taxonomy id: 10090
Gene id: 14104
7Acot21acyl-coenzyme A thioesterase 2, mitochondrial precursor [Mus musculus] [gi:238624114]
Taxonomy id: 10090
Gene id: 171210
8Acot11acyl-coenzyme A thioesterase 1 [Mus musculus] [gi:6753550]
Taxonomy id: 10090
Gene id: 26897
9Pafah1b21platelet-activating factor acetylhydrolase IB subunit beta [Mus musculus] [gi:40254624]
Taxonomy id: 10090
Gene id: 18475
10Apeh1Acylpeptide hydrolase [Mus musculus] [gi:19343726]
Taxonomy id: 10090
Gene id: 235606
11Lypla11acyl-protein thioesterase 1 [Mus musculus] [gi:6678760]
Taxonomy id: 10090
Gene id: 18777
12Pme11protein phosphatase methylesterase 1 [Mus musculus] [gi:30794138]
Taxonomy id: 10090
Gene id: 72590
13Abhd101abhydrolase domain-containing protein 10, mitochondrial precursor [Mus musculus] [gi:269784760]
Taxonomy id: 10090
Gene id: 213012
14Rbbp91putative hydrolase RBBP9 [Mus musculus] [gi:86439977]
Taxonomy id: 10090
Gene id: 26450
15Es11liver carboxylesterase N precursor [Mus musculus] [gi:247269929]
Taxonomy id: 10090
Gene id: 13884
16Acot71cytosolic acyl coenzyme A thioester hydrolase isoform 1 [Mus musculus] [gi:225690616]
Taxonomy id: 10090
Gene id: 70025
17Dpp91dipeptidyl peptidase 9 [Mus musculus] [gi:255003757]
Taxonomy id: 10090
Gene id: 224897
18Dpp81dipeptidyl peptidase 8 [Mus musculus] [gi:31542571]
Taxonomy id: 10090
Gene id: 74388
19Pafah1b31platelet-activating factor acetylhydrolase IB subunit gamma [Mus musculus] [gi:6679201]
Taxonomy id: 10090
Gene id: 18476
20Magl1monoglyceride lipase isoform d [Mus musculus] [gi:261878511]
Taxonomy id: 10090
Gene id: 23945
21Abhd41abhydrolase domain-containing protein 4 isoform 1 [Mus musculus] [gi:326937491]
Taxonomy id: 10090
Gene id: 105501
22Abhd111abhydrolase domain-containing protein 11 isoform 1 [Mus musculus] [gi:21644577]
Taxonomy id: 10090
Gene id: 68758
23Pla2g151group XV phospholipase A2 precursor [Mus musculus] [gi:19527008]
Taxonomy id: 10090
Gene id: 192654
24Lyplal11lysophospholipase-like protein 1 [Mus musculus] [gi:227496223]
Taxonomy id: 10090
Gene id: 226791
25Lipe1hormone-sensitive lipase isoform 2 [Mus musculus] [gi:87239972]
Taxonomy id: 10090
Gene id: 16890
26Tpp21Tpp2 protein [Mus musculus] [gi:37194903]
Taxonomy id: 10090
Gene id: 22019

§ Panel component ID.
Protocol
Assay Overview:

The purpose of this assay is to determine the selectivity profile of powder samples of test compounds in mouse brain soluble proteome by activity-based protein profiling (ABPP) in combination with the multidimensional protein identification technology (MudPIT) liquid chromatography tandem mass spectrometry (LC-M/MS) analysis platform. In this assay, paired complex proteome samples are treated with test compound or DMSO, followed by reaction with the serine hydrolase specific activity-based fluorophosphonate-biotin (FP-biotin) affinity probe. Biotinylated proteins are enriched, trypsinized, and analyzed by MudPIT. Inhibition of target and anti-target activity is quantified by comparing spectra counts between the test compound- and DMSO-treated samples. As designed, compounds that act as inhibitors will block FP-biotin labeling, reducing enrichment in the inhibitor-treated sample relative to the DMSO-treated sample, thus giving a smaller spectra count ratio (test compound/DMSO) for each protein. Proteins not targeted by inhibitors would be expected to have a ratio of 1.

Protocol Summary:

Sample Preparation: paired aliquots of mouse brain soluble proteome (1 mL of 1 mg/mL in 50 mM Tris pH 8.0 [Tris] buffer) were treated with either 1 uM test compound (20 uL of a 50x stock in DMSO) or DMSO for 30 minutes at 37 C. Each reaction was labeled with 5 uM of FP-biotin (5 uL of a 50x stock in DMSO) for 2 hours at 25 C. TritonX100 was added to 1% final concentration (v/v), and the proteomes were desalted over PD-10 desalting columns (GE Healthcare). SDS was added to the eluate to give 0.5% final concentration, and the samples heated to 90 C for 8 minutes. FP-labeled proteins were enriched with streptavidin beads (100 uL of 50% slurry; 25 C, 1 hour, 8.5 mL total volume Tris). Following enrichment, the beads were washed with 1% SDS in Tris (2x), 6 M Urea in Tris (2x), and Tris (2x), and then resuspended in 8 M urea, reduced with TCEP (10 mM) for 25 minutes at 25 C, and alkylated with iodoacetamide (12.5 mM) for 25 minutes at 25 C in the dark. The urea concentration was reduced to 2 M with Tris, and on-bead digestions were performed for 12 hours at 37 C with sequence-grade modified trypsin (Promega; 2 ug) in the presence of 2 mM CaCl2. Peptide samples were acidified to a final concentration of 5% (v/v) formic acid, pressure-loaded on to a biphasic (strong cation exchange/reversed phase) MudPIT capillary column and analyzed as described below. A total of 4 biological replicates were prepared and analyzed for each experiment.

LC-MS/MS analysis: digested and acidified peptide mixtures were analyzed by two-dimensional liquid chromatography/tandem mass spectrometry (MudPIT) using an Agilent 1200-series quaternary pump and Thermo Scientific LTQ ion trap mass spectrometer. Peptides were eluted in a 5-step MudPIT experiment using 0%, 25%, 50%, 80%, and 100% salt bumps of 500 mM aqueous ammonium acetate, with chromatographic elution following each salt pulse accomplished using an increasing gradient of aqueous acetonitrile containing 0.1% formic acid over 125 minutes. Data were collected in data-dependent acquisition mode with dynamic exclusion turned on (90 s, repeat of 1). Specifically, one full MS (MS1) scan (400-1800 m/z) was followed by 7 MS2 scans of the most abundant ions. The MS2 spectra data were extracted from the raw file using RAW Xtractor (version 1.9.1; publically available at http://fields.scripps.edu/downloads.php). MS2 spectra data were searched using the PROLUCID algorithm (publically available at http://fields.scripps.edu/downloads.php) against the latest version of the mouse UniProt database concatenated with the reversed database for assessment of false-discovery rates. PROLUCID searches allowed for variable oxidation of methionine (+16), static modification of cysteine (+57 due to alkylation), and no enzyme specificity. The resulting MS2 spectra matches were assembled into protein identifications and filtered using DTASelect (version 2.0.41) using the -trypstat and --modstat options, which apply different statistical models for the analysis of tryptic, half-tryptic, non-tryptic, and modified peptides. DTASelect 2.0 uses a quadratic discriminant analysis to maintain a user-defined false positive hit rate (as determined by number of hits against the reversed database) below 1% at the peptide level. Spectra count abundance ratios (test_compound/DMSO) were calculated for all serine hydrolases with an average of 10 or more spectra counts in the DMSO control samples.

Ratio = Average_Spectra_Counts_Test_Compound / Average_Spectra_Counts_DMSO_Control

Where:

Average_Spectra_Counts_Test_Compound is the average number of spectra counts for a given serine hydrolase across all test compound-treated samples.
Average_Spectra_Counts_DMSO_Control is the average number of spectra counts for a given serine hydrolase across all DMSO control samples.

PubChem Activity Outcome and Score:

The following applies to each panel in this assay:

A compound was considered active for a particular target/anti-target with an abundance ratio of less than or equal to 0.5. A compound was considered inactive for a specified target/anti-target with an abundance ratio of greater than 0.5.

Overall Outcome and Score:

A compound was considered active if it was active for pPAFAH (aka Pla2g7) and active for fewer than 2 serine hydrolase anti-targets. A compound was considered inactive if it was inactive for pPAFAH (aka Pla2g7) and/or active for 2 or more serine hydrolase anti-targets.

The PubChem Activity Score is assigned a value of 100 for active compounds, and 0 for inactive compounds.

The PubChem Activity Score range for active compounds is 100-100. There are no inactive compounds.

List of Reagents:

Mouse brain soluble proteome (provided by assay provider)
FP-biotin (provided by Assay Provider)
Tris-HCl (SigmaAldrich T5941)
Triton-X100 (Fisher AC21568-0010)
PD-10 desalting columns (GE Healthcare 17-0851-01)
SDS (SigmaAldrich L6026)
Urea (Fisher U15-3)
TCEP (SigmaAldrich 75259)
Iodoacetamide (iodoacetamide; SigmaAldrich I1149)
Trypsin (Promega V5111)
CaCl2 (SigmaAldrich C1016)
Streptavidin beads (Pierce 20349)
Formic acid (Fluka 06440)
Fused-silica (Agilent 160-2635-10)
Strong cation exchange (Phenomenex CH0-2257)
Aqua C18 (Phenomenex 04A-4299)
Acetonitrile (Fisher A955-4)
Millipore-filtered Water
Ammonium bicarbonate (Acros, AC37093-0010)
Comment
This assay was performed by the assay provider with powder samples of test compounds.
Categorized Comment
BAO: version: 1.4b1090

BAO: bioassay specification: assay stage: secondary: mmoa characterization

BAO: bioassay specification: assay biosafety level: bsl1

BAO: assay format: biochemical format: protein format: single protein format

BAO: bioassay specification: assay measurement type: endpoint assay

BAO: bioassay specification: assay readout content: assay readout method: regular screening

BAO: bioassay specification: assay readout content: content readout type: single readout

BAO: meta target: molecular target: protein target: enzyme: generic hydrolase

BAO: meta target detail: binding reporter specification: interaction: protein-small molecule

Result Definitions
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TIDNameDescriptionPID§Panel TargetsHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1Outcome [Pla2g7]One of Active, Inactive, or Not Tested.1platelet-activating factor acetylhydrolase [Mus musculus]Outcome
2Spectra Counts [1] [Pla2g7] (1μM**)Number of spectra counts for Pla2g7 in test compound treated sample, replicate 1.1Integer
3Spectra Counts [2] [Pla2g7] (1μM**)Number of spectra counts for Pla2g7 in test compound treated sample, replicate 2.1Integer
4Spectra Counts [3] [Pla2g7] (1μM**)Number of spectra counts for Pla2g7 in test compound treated sample, replicate 3.1Integer
5Spectra Counts [4] [Pla2g7] (1μM**)Number of spectra counts for Pla2g7 in test compound treated sample, replicate 4.1Integer
6Average Spectra Counts [Pla2g7] (1μM**)Average number of spectra counts for Pla2g7 in test compound treated samples.1Integer
7Standard Deviation [Pla2g7]Standard Deviation for the average number of spectra counts for Pla2g7 in test compound treated samples.1Float
8Spectra Counts [1] [DMSO control, Pla2g7]Number of spectra counts for Pla2g7 in DMSO control sample, replicate 1.1Integer
9Spectra Counts [2] [DMSO control, Pla2g7]Number of spectra counts for Pla2g7 in DMSO control sample, replicate 2.1Integer
10Spectra Counts [3] [DMSO control, Pla2g7]Number of spectra counts for Pla2g7 in DMSO control sample, replicate 3.1Integer
11Spectra Counts [4] [DMSO control, Pla2g7]Number of spectra counts for Pla2g7 in DMSO control sample, replicate 4.1Integer
12Average Spectra Counts [DMSO control, Pla2g7]Average number of spectra counts for Pla2g7 in DMSO control samples.1Integer
13Standard Deviation [DMSO control, Pla2g7]Standard deviation for the average number of spectra counts for Pla2g7 in DMSO control samples.1Float
14Ratio Average Spectra Counts [Pla2g7]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.1Floatratio
15Outcome [Esd]One of Active, Inactive, or Not Tested.2S-formylglutathione hydrolase [Mus musculus]Outcome
16Spectra Counts [1] [Esd] (1μM**)Number of spectra counts for Esd in test compound treated sample, replicate 1.2Integer
17Spectra Counts [2] [Esd] (1μM**)Number of spectra counts for Esd in test compound treated sample, replicate 2.2Integer
18Spectra Counts [3] [Esd] (1μM**)Number of spectra counts for Esd in test compound treated sample, replicate 3.2Integer
19Spectra Counts [4] [Esd] (1μM**)Number of spectra counts for Esd in test compound treated sample, replicate 4.2Integer
20Average Spectra Counts [Esd] (1μM**)Average number of spectra counts for Esd in test compound treated samples.2Integer
21Standard Deviation [Esd]Standard Deviation for the average number of spectra counts for Esd in test compound treated samples.2Float
22Spectra Counts [1] [DMSO control, Esd]Number of spectra counts for Esd in DMSO control sample, replicate 1.2Integer
23Spectra Counts [2] [DMSO control, Esd]Number of spectra counts for Esd in DMSO control sample, replicate 2.2Integer
24Spectra Counts [3] [DMSO control, Esd]Number of spectra counts for Esd in DMSO control sample, replicate 3.2Integer
25Spectra Counts [4] [DMSO control, Esd]Number of spectra counts for Esd in DMSO control sample, replicate 4.2Integer
26Average Spectra Counts [DMSO control, Esd]Average number of spectra counts for Esd in DMSO control samples.2Integer
27Standard Deviation [DMSO control, Esd]Standard deviation for the average number of spectra counts for Esd in DMSO control samples.2Float
28Ratio Average Spectra Counts [Esd]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.2Floatratio
29Outcome [Prep]One of Active, Inactive, or Not Tested.3prolyl endopeptidase [Mus musculus]Outcome
30Spectra Counts [1] [Prep] (1μM**)Number of spectra counts for Prep in test compound treated sample, replicate 1.3Integer
31Spectra Counts [2] [Prep] (1μM**)Number of spectra counts for Prep in test compound treated sample, replicate 2.3Integer
32Spectra Counts [3] [Prep] (1μM**)Number of spectra counts for Prep in test compound treated sample, replicate 3.3Integer
33Spectra Counts [4] [Prep] (1μM**)Number of spectra counts for Prep in test compound treated sample, replicate 4.3Integer
34Average Spectra Counts [Prep] (1μM**)Average number of spectra counts for Prep in test compound treated samples.3Integer
35Standard Deviation [Prep]Standard Deviation for the average number of spectra counts for Prep in test compound treated samples.3Float
36Spectra Counts [1] [DMSO control, Prep]Number of spectra counts for Prep in DMSO control sample, replicate 1.3Integer
37Spectra Counts [2] [DMSO control, Prep]Number of spectra counts for Prep in DMSO control sample, replicate 2.3Integer
38Spectra Counts [3] [DMSO control, Prep]Number of spectra counts for Prep in DMSO control sample, replicate 3.3Integer
39Spectra Counts [4] [DMSO control, Prep]Number of spectra counts for Prep in DMSO control sample, replicate 4.3Integer
40Average Spectra Counts [DMSO control, Prep]Average number of spectra counts for Prep in DMSO control samples.3Integer
41Standard Deviation [DMSO control, Prep]Standard deviation for the average number of spectra counts for Prep in DMSO control samples.3Float
42Ratio Average Spectra Counts [Prep]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.3Floatratio
43Outcome [Prepl]One of Active, Inactive, or Not Tested.4prolyl endopeptidase-like isoform a [Mus musculus]Outcome
44Spectra Counts [1] [Prepl] (1μM**)Number of spectra counts for Prepl in test compound treated sample, replicate 1.4Integer
45Spectra Counts [2] [Prepl] (1μM**)Number of spectra counts for Prepl in test compound treated sample, replicate 2.4Integer
46Spectra Counts [3] [Prepl] (1μM**)Number of spectra counts for Prepl in test compound treated sample, replicate 3.4Integer
47Spectra Counts [4] [Prepl] (1μM**)Number of spectra counts for Prepl in test compound treated sample, replicate 4.4Integer
48Average Spectra Counts [Prepl] (1μM**)Average number of spectra counts for Prepl in test compound treated samples.4Integer
49Standard Deviation [Prepl]Standard Deviation for the average number of spectra counts for Prepl in test compound treated samples.4Float
50Spectra Counts [1] [DMSO control, Prepl]Number of spectra counts for Prepl in DMSO control sample, replicate 1.4Integer
51Spectra Counts [2] [DMSO control, Prepl]Number of spectra counts for Prepl in DMSO control sample, replicate 2.4Integer
52Spectra Counts [3] [DMSO control, Prepl]Number of spectra counts for Prepl in DMSO control sample, replicate 3.4Integer
53Spectra Counts [4] [DMSO control, Prepl]Number of spectra counts for Prepl in DMSO control sample, replicate 4.4Integer
54Average Spectra Counts [DMSO control, Prepl]Average number of spectra counts for Prepl in DMSO control samples.4Integer
55Standard Deviation [DMSO control, Prepl]Standard deviation for the average number of spectra counts for Prepl in DMSO control samples.4Float
56Ratio Average Spectra Counts [Prepl]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.4Floatratio
57Outcome [Lypla2]One of Active, Inactive, or Not Tested.5acyl-protein thioesterase 2 [Mus musculus]Outcome
58Spectra Counts [1] [Lypla2] (1μM**)Number of spectra counts for Lypla2 in test compound treated sample, replicate 1.5Integer
59Spectra Counts [2] [Lypla2] (1μM**)Number of spectra counts for Lypla2 in test compound treated sample, replicate 2.5Integer
60Spectra Counts [3] [Lypla2] (1μM**)Number of spectra counts for Lypla2 in test compound treated sample, replicate 3.5Integer
61Spectra Counts [4] [Lypla2] (1μM**)Number of spectra counts for Lypla2 in test compound treated sample, replicate 4.5Integer
62Average Spectra Counts [Lypla2] (1μM**)Average number of spectra counts for Lypla2 in test compound treated samples.5Integer
63Standard Deviation [Lypla2]Standard Deviation for the average number of spectra counts for Lypla2 in test compound treated samples.5Float
64Spectra Counts [1] [DMSO control, Lypla2]Number of spectra counts for Lypla2 in DMSO control sample, replicate 1.5Integer
65Spectra Counts [2] [DMSO control, Lypla2]Number of spectra counts for Lypla2 in DMSO control sample, replicate 2.5Integer
66Spectra Counts [3] [DMSO control, Lypla2]Number of spectra counts for Lypla2 in DMSO control sample, replicate 3.5Integer
67Spectra Counts [4] [DMSO control, Lypla2]Number of spectra counts for Lypla2 in DMSO control sample, replicate 4.5Integer
68Average Spectra Counts [DMSO control, Lypla2]Average number of spectra counts for Lypla2 in DMSO control samples.5Integer
69Standard Deviation [DMSO control, Lypla2]Standard deviation for the average number of spectra counts for Lypla2 in DMSO control samples.5Float
70Ratio Average Spectra Counts [Lypla2]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.5Floatratio
71Outcome [Fasn]One of Active, Inactive, or Not Tested.6fatty acid synthase [Mus musculus]Outcome
72Spectra Counts [1] [Fasn] (1μM**)Number of spectra counts for Fasn in test compound treated sample, replicate 1.6Integer
73Spectra Counts [2] [Fasn] (1μM**)Number of spectra counts for Fasn in test compound treated sample, replicate 2.6Integer
74Spectra Counts [3] [Fasn] (1μM**)Number of spectra counts for Fasn in test compound treated sample, replicate 3.6Integer
75Spectra Counts [4] [Fasn] (1μM**)Number of spectra counts for Fasn in test compound treated sample, replicate 4.6Integer
76Average Spectra Counts [Fasn] (1μM**)Average number of spectra counts for Fasn in test compound treated samples.6Integer
77Standard Deviation [Fasn]Standard Deviation for the average number of spectra counts for Fasn in test compound treated samples.6Float
78Spectra Counts [1] [DMSO control, Fasn]Number of spectra counts for Fasn in DMSO control sample, replicate 1.6Integer
79Spectra Counts [2] [DMSO control, Fasn]Number of spectra counts for Fasn in DMSO control sample, replicate 2.6Integer
80Spectra Counts [3] [DMSO control, Fasn]Number of spectra counts for Fasn in DMSO control sample, replicate 3.6Integer
81Spectra Counts [4] [DMSO control, Fasn]Number of spectra counts for Fasn in DMSO control sample, replicate 4.6Integer
82Average Spectra Counts [DMSO control, Fasn]Average number of spectra counts for Fasn in DMSO control samples.6Integer
83Standard Deviation [DMSO control, Fasn]Standard deviation for the average number of spectra counts for Fasn in DMSO control samples.6Float
84Ratio Average Spectra Counts [Fasn]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.6Floatratio
85Outcome [Acot2]One of Active, Inactive, or Not Tested.7acyl-coenzyme A thioesterase 2, mitochondrial precursor [Mus musculus]Outcome
86Spectra Counts [1] [Acot2] (1μM**)Number of spectra counts for Acot2 in test compound treated sample, replicate 1.7Integer
87Spectra Counts [2] [Acot2] (1μM**)Number of spectra counts for Acot2 in test compound treated sample, replicate 2.7Integer
88Spectra Counts [3] [Acot2] (1μM**)Number of spectra counts for Acot2 in test compound treated sample, replicate 3.7Integer
89Spectra Counts [4] [Acot2] (1μM**)Number of spectra counts for Acot2 in test compound treated sample, replicate 4.7Integer
90Average Spectra Counts [Acot2] (1μM**)Average number of spectra counts for Acot2 in test compound treated samples.7Integer
91Standard Deviation [Acot2]Standard Deviation for the average number of spectra counts for Acot2 in test compound treated samples.7Float
92Spectra Counts [1] [DMSO control, Acot2]Number of spectra counts for Acot2 in DMSO control sample, replicate 1.7Integer
93Spectra Counts [2] [DMSO control, Acot2]Number of spectra counts for Acot2 in DMSO control sample, replicate 2.7Integer
94Spectra Counts [3] [DMSO control, Acot2]Number of spectra counts for Acot2 in DMSO control sample, replicate 3.7Integer
95Spectra Counts [4] [DMSO control, Acot2]Number of spectra counts for Acot2 in DMSO control sample, replicate 4.7Integer
96Average Spectra Counts [DMSO control, Acot2]Average number of spectra counts for Acot2 in DMSO control samples.7Integer
97Standard Deviation [DMSO control, Acot2]Standard deviation for the average number of spectra counts for Acot2 in DMSO control samples.7Float
98Ratio Average Spectra Counts [Acot2]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.7Floatratio
99Outcome [Acot1]One of Active, Inactive, or Not Tested.8acyl-coenzyme A thioesterase 1 [Mus musculus]Outcome
100Spectra Counts [1] [Acot1] (1μM**)Number of spectra counts for Acot1 in test compound treated sample, replicate 1.8Integer
101Spectra Counts [2] [Acot1] (1μM**)Number of spectra counts for Acot1 in test compound treated sample, replicate 2.8Integer
102Spectra Counts [3] [Acot1] (1μM**)Number of spectra counts for Acot1 in test compound treated sample, replicate 3.8Integer
103Spectra Counts [4] [Acot1] (1μM**)Number of spectra counts for Acot1 in test compound treated sample, replicate 4.8Integer
104Average Spectra Counts [Acot1] (1μM**)Average number of spectra counts for Acot1 in test compound treated samples.8Integer
105Standard Deviation [Acot1]Standard Deviation for the average number of spectra counts for Acot1 in test compound treated samples.8Float
106Spectra Counts [1] [DMSO control, Acot1]Number of spectra counts for Acot1 in DMSO control sample, replicate 1.8Integer
107Spectra Counts [2] [DMSO control, Acot1]Number of spectra counts for Acot1 in DMSO control sample, replicate 2.8Integer
108Spectra Counts [3] [DMSO control, Acot1]Number of spectra counts for Acot1 in DMSO control sample, replicate 3.8Integer
109Spectra Counts [4] [DMSO control, Acot1]Number of spectra counts for Acot1 in DMSO control sample, replicate 4.8Integer
110Average Spectra Counts [DMSO control, Acot1]Average number of spectra counts for Acot1 in DMSO control samples.8Integer
111Standard Deviation [DMSO control, Acot1]Standard deviation for the average number of spectra counts for Acot1 in DMSO control samples.8Float
112Ratio Average Spectra Counts [Acot1]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.8Floatratio
113Outcome [Pafah1b2]One of Active, Inactive, or Not Tested.9platelet-activating factor acetylhydrolase IB subunit beta [Mus musculus]Outcome
114Spectra Counts [1] [Pafah1b2] (1μM**)Number of spectra counts for Pafah1b2 in test compound treated sample, replicate 1.9Integer
115Spectra Counts [2] [Pafah1b2] (1μM**)Number of spectra counts for Pafah1b2 in test compound treated sample, replicate 2.9Integer
116Spectra Counts [3] [Pafah1b2] (1μM**)Number of spectra counts for Pafah1b2 in test compound treated sample, replicate 3.9Integer
117Spectra Counts [4] [Pafah1b2] (1μM**)Number of spectra counts for Pafah1b2 in test compound treated sample, replicate 4.9Integer
118Average Spectra Counts [Pafah1b2] (1μM**)Average number of spectra counts for Pafah1b2 in test compound treated samples.9Integer
119Standard Deviation [Pafah1b2]Standard Deviation for the average number of spectra counts for Pafah1b2 in test compound treated samples.9Float
120Spectra Counts [1] [DMSO control, Pafah1b2]Number of spectra counts for Pafah1b2 in DMSO control sample, replicate 1.9Integer
121Spectra Counts [2] [DMSO control, Pafah1b2]Number of spectra counts for Pafah1b2 in DMSO control sample, replicate 2.9Integer
122Spectra Counts [3] [DMSO control, Pafah1b2]Number of spectra counts for Pafah1b2 in DMSO control sample, replicate 3.9Integer
123Spectra Counts [4] [DMSO control, Pafah1b2]Number of spectra counts for Pafah1b2 in DMSO control sample, replicate 4.9Integer
124Average Spectra Counts [DMSO control, Pafah1b2]Average number of spectra counts for Pafah1b2 in DMSO control samples.9Integer
125Standard Deviation [DMSO control, Pafah1b2]Standard deviation for the average number of spectra counts for Pafah1b2 in DMSO control samples.9Float
126Ratio Average Spectra Counts [Pafah1b2]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.9Floatratio
127Outcome [Apeh]One of Active, Inactive, or Not Tested.10Acylpeptide hydrolase [Mus musculus]Outcome
128Spectra Counts [1] [Apeh] (1μM**)Number of spectra counts for Apeh in test compound treated sample, replicate 1.10Integer
129Spectra Counts [2] [Apeh] (1μM**)Number of spectra counts for Apeh in test compound treated sample, replicate 2.10Integer
130Spectra Counts [3] [Apeh] (1μM**)Number of spectra counts for Apeh in test compound treated sample, replicate 3.10Integer
131Spectra Counts [4] [Apeh] (1μM**)Number of spectra counts for Apeh in test compound treated sample, replicate 4.10Integer
132Average Spectra Counts [Apeh] (1μM**)Average number of spectra counts for Apeh in test compound treated samples.10Integer
133Standard Deviation [Apeh]Standard Deviation for the average number of spectra counts for Apeh in test compound treated samples.10Float
134Spectra Counts [1] [DMSO control, Apeh]Number of spectra counts for Apeh in DMSO control sample, replicate 1.10Integer
135Spectra Counts [2] [DMSO control, Apeh]Number of spectra counts for Apeh in DMSO control sample, replicate 2.10Integer
136Spectra Counts [3] [DMSO control, Apeh]Number of spectra counts for Apeh in DMSO control sample, replicate 3.10Integer
137Spectra Counts [4] [DMSO control, Apeh]Number of spectra counts for Apeh in DMSO control sample, replicate 4.10Integer
138Average Spectra Counts [DMSO control, Apeh]Average number of spectra counts for Apeh in DMSO control samples.10Integer
139Standard Deviation [DMSO control, Apeh]Standard deviation for the average number of spectra counts for Apeh in DMSO control samples.10Float
140Ratio Average Spectra Counts [Apeh]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.10Floatratio
141Outcome [Lypla1]One of Active, Inactive, or Not Tested.11acyl-protein thioesterase 1 [Mus musculus]Outcome
142Spectra Counts [1] [Lypla1] (1μM**)Number of spectra counts for Lypla1 in test compound treated sample, replicate 1.11Integer
143Spectra Counts [2] [Lypla1] (1μM**)Number of spectra counts for Lypla1 in test compound treated sample, replicate 2.11Integer
144Spectra Counts [3] [Lypla1] (1μM**)Number of spectra counts for Lypla1 in test compound treated sample, replicate 3.11Integer
145Spectra Counts [4] [Lypla1] (1μM**)Number of spectra counts for Lypla1 in test compound treated sample, replicate 4.11Integer
146Average Spectra Counts [Lypla1] (1μM**)Average number of spectra counts for Lypla1 in test compound treated samples.11Integer
147Standard Deviation [Lypla1]Standard Deviation for the average number of spectra counts for Lypla1 in test compound treated samples.11Float
148Spectra Counts [1] [DMSO control, Lypla1]Number of spectra counts for Lypla1 in DMSO control sample, replicate 1.11Integer
149Spectra Counts [2] [DMSO control, Lypla1]Number of spectra counts for Lypla1 in DMSO control sample, replicate 2.11Integer
150Spectra Counts [3] [DMSO control, Lypla1]Number of spectra counts for Lypla1 in DMSO control sample, replicate 3.11Integer
151Spectra Counts [4] [DMSO control, Lypla1]Number of spectra counts for Lypla1 in DMSO control sample, replicate 4.11Integer
152Average Spectra Counts [DMSO control, Lypla1]Average number of spectra counts for Lypla1 in DMSO control samples.11Integer
153Standard Deviation [DMSO control, Lypla1]Standard deviation for the average number of spectra counts for Lypla1 in DMSO control samples.11Float
154Ratio Average Spectra Counts [Lypla1]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.11Floatratio
155Outcome [Pme1]One of Active, Inactive, or Not Tested.12protein phosphatase methylesterase 1 [Mus musculus]Outcome
156Spectra Counts [1] [Pme1] (1μM**)Number of spectra counts for Pme1 in test compound treated sample, replicate 1.12Integer
157Spectra Counts [2] [Pme1] (1μM**)Number of spectra counts for Pme1 in test compound treated sample, replicate 2.12Integer
158Spectra Counts [3] [Pme1] (1μM**)Number of spectra counts for Pme1 in test compound treated sample, replicate 3.12Integer
159Spectra Counts [4] [Pme1] (1μM**)Number of spectra counts for Pme1 in test compound treated sample, replicate 4.12Integer
160Average Spectra Counts [Pme1] (1μM**)Average number of spectra counts for Pme1 in test compound treated samples.12Integer
161Standard Deviation [Pme1]Standard Deviation for the average number of spectra counts for Pme1 in test compound treated samples.12Float
162Spectra Counts [1] [DMSO control, Pme1]Number of spectra counts for Pme1 in DMSO control sample, replicate 1.12Integer
163Spectra Counts [2] [DMSO control, Pme1]Number of spectra counts for Pme1 in DMSO control sample, replicate 2.12Integer
164Spectra Counts [3] [DMSO control, Pme1]Number of spectra counts for Pme1 in DMSO control sample, replicate 3.12Integer
165Spectra Counts [4] [DMSO control, Pme1]Number of spectra counts for Pme1 in DMSO control sample, replicate 4.12Integer
166Average Spectra Counts [DMSO control, Pme1]Average number of spectra counts for Pme1 in DMSO control samples.12Integer
167Standard Deviation [DMSO control, Pme1]Standard deviation for the average number of spectra counts for Pme1 in DMSO control samples.12Float
168Ratio Average Spectra Counts [Pme1]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.12Floatratio
169Outcome [Abdh10]One of Active, Inactive, or Not Tested.13abhydrolase domain-containing protein 10, mitochondrial precursor [Mus musculus]Outcome
170Spectra Counts [1] [Abdh10] (1μM**)Number of spectra counts for Abhd10 in test compound treated sample, replicate 1.13Integer
171Spectra Counts [2] [Abdh10] (1μM**)Number of spectra counts for Abhd10 in test compound treated sample, replicate 2.13Integer
172Spectra Counts [3] [Abdh10] (1μM**)Number of spectra counts for Abhd10 in test compound treated sample, replicate 3.13Integer
173Spectra Counts [4] [Abdh10] (1μM**)Number of spectra counts for Abhd10 in test compound treated sample, replicate 4.13Integer
174Average Spectra Counts [Abdh10] (1μM**)Average number of spectra counts for Abhd10 in test compound treated samples.13Integer
175Standard Deviation [Abdh10]Standard Deviation for the average number of spectra counts for Abhd10 in test compound treated samples.13Float
176Spectra Counts [1] [DMSO control, Abdh10]Number of spectra counts for Abhd10 in DMSO control sample, replicate 1.13Integer
177Spectra Counts [2] [DMSO control, Abdh10]Number of spectra counts for Abhd10 in DMSO control sample, replicate 2.13Integer
178Spectra Counts [3] [DMSO control, Abdh10]Number of spectra counts for Abhd10 in DMSO control sample, replicate 3.13Integer
179Spectra Counts [4] [DMSO control, Abdh10]Number of spectra counts for Abhd10 in DMSO control sample, replicate 4.13Integer
180Average Spectra Counts [DMSO control, Abdh10]Average number of spectra counts for Abhd10 in DMSO control samples.13Integer
181Standard Deviation [DMSO control, Abdh10]Standard deviation for the average number of spectra counts for Abhd10 in DMSO control samples.13Float
182Ratio Average Spectra Counts [Abdh10]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.13Floatratio
183Outcome [Rbbp9]One of Active, Inactive, or Not Tested.14putative hydrolase RBBP9 [Mus musculus]Outcome
184Spectra Counts [1] [Rbbp9] (1μM**)Number of spectra counts for Rbbp9 in test compound treated sample, replicate 1.14Integer
185Spectra Counts [2] [Rbbp9] (1μM**)Number of spectra counts for Rbbp9 in test compound treated sample, replicate 2.14Integer
186Spectra Counts [3] [Rbbp9] (1μM**)Number of spectra counts for Rbbp9 in test compound treated sample, replicate 3.14Integer
187Spectra Counts [4] [Rbbp9] (1μM**)Number of spectra counts for Rbbp9 in test compound treated sample, replicate 4.14Integer
188Average Spectra Counts [Rbbp9] (1μM**)Average number of spectra counts for Rbbp9 in test compound treated samples.14Integer
189Standard Deviation [Rbbp9]Standard Deviation for the average number of spectra counts for Rbbp9 in test compound treated samples.14Float
190Spectra Counts [1] [DMSO control, Rbbp9]Number of spectra counts for Rbbp9 in DMSO control sample, replicate 1.14Integer
191Spectra Counts [2] [DMSO control, Rbbp9]Number of spectra counts for Rbbp9 in DMSO control sample, replicate 2.14Integer
192Spectra Counts [3] [DMSO control, Rbbp9]Number of spectra counts for Rbbp9 in DMSO control sample, replicate 3.14Integer
193Spectra Counts [4] [DMSO control, Rbbp9]Number of spectra counts for Rbbp9 in DMSO control sample, replicate 4.14Integer
194Average Spectra Counts [DMSO control, Rbbp9]Average number of spectra counts for Rbbp9 in DMSO control samples.14Integer
195Standard Deviation [DMSO control, Rbbp9]Standard deviation for the average number of spectra counts for Rbbp9 in DMSO control samples.14Float
196Ratio Average Spectra Counts [Rbbp9]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.14Floatratio
197Outcome [Es1]One of Active, Inactive, or Not Tested.15liver carboxylesterase N precursor [Mus musculus]Outcome
198Spectra Counts [1] [Es1] (1μM**)Number of spectra counts for Es1 in test compound treated sample, replicate 1.15Integer
199Spectra Counts [2] [Es1] (1μM**)Number of spectra counts for Es1 in test compound treated sample, replicate 2.15Integer
200Spectra Counts [3] [Es1] (1μM**)Number of spectra counts for Es1 in test compound treated sample, replicate 3.15Integer
201Spectra Counts [4] [Es1] (1μM**)Number of spectra counts for Es1 in test compound treated sample, replicate 4.15Integer
202Average Spectra Counts [Es1] (1μM**)Average number of spectra counts for Es1 in test compound treated samples.15Integer
203Standard Deviation [Es1]Standard Deviation for the average number of spectra counts for Es1 in test compound treated samples.15Float
204Spectra Counts [1] [DMSO control, Es1]Number of spectra counts for Es1 in DMSO control sample, replicate 1.15Integer
205Spectra Counts [2] [DMSO control, Es1]Number of spectra counts for Es1 in DMSO control sample, replicate 2.15Integer
206Spectra Counts [3] [DMSO control, Es1]Number of spectra counts for Es1 in DMSO control sample, replicate 3.15Integer
207Spectra Counts [4] [DMSO control, Es1]Number of spectra counts for Es1 in DMSO control sample, replicate 4.15Integer
208Average Spectra Counts [DMSO control, Es1]Average number of spectra counts for Es1 in DMSO control samples.15Integer
209Standard Deviation [DMSO control, Es1]Standard deviation for the average number of spectra counts for Es1 in DMSO control samples.15Float
210Ratio Average Spectra Counts [Es1]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.15Floatratio
211Outcome [Acot7]One of Active, Inactive, or Not Tested.16cytosolic acyl coenzyme A thioester hydrolase isoform 1 [Mus musculus]Outcome
212Spectra Counts [1] [Acot7] (1μM**)Number of spectra counts for Acot7 in test compound treated sample, replicate 1.16Integer
213Spectra Counts [2] [Acot7] (1μM**)Number of spectra counts for Acot7 in test compound treated sample, replicate 2.16Integer
214Spectra Counts [3] [Acot7] (1μM**)Number of spectra counts for Acot7 in test compound treated sample, replicate 3.16Integer
215Spectra Counts [4] [Acot7] (1μM**)Number of spectra counts for Acot7 in test compound treated sample, replicate 4.16Integer
216Average Spectra Counts [Acot7] (1μM**)Average number of spectra counts for Acot7 in test compound treated samples.16Integer
217Standard Deviation [Acot7]Standard Deviation for the average number of spectra counts for Acot7 in test compound treated samples.16Float
218Spectra Counts [1] [DMSO control, Acot7]Number of spectra counts for Acot7 in DMSO control sample, replicate 1.16Integer
219Spectra Counts [2] [DMSO control, Acot7]Number of spectra counts for Acot7 in DMSO control sample, replicate 2.16Integer
220Spectra Counts [3] [DMSO control, Acot7]Number of spectra counts for Acot7 in DMSO control sample, replicate 3.16Integer
221Spectra Counts [4] [DMSO control, Acot7]Number of spectra counts for Acot7 in DMSO control sample, replicate 4.16Integer
222Average Spectra Counts [DMSO control, Acot7]Average number of spectra counts for Acot7 in DMSO control samples.16Integer
223Standard Deviation [DMSO control, Acot7]Standard deviation for the average number of spectra counts for Acot7 in DMSO control samples.16Float
224Ratio Average Spectra Counts [Acot7]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.16Floatratio
225Outcome [Dpp9]One of Active, Inactive, or Not Tested.17dipeptidyl peptidase 9 [Mus musculus]Outcome
226Spectra Counts [1] [Dpp9] (1μM**)Number of spectra counts for Dpp9 in test compound treated sample, replicate 1.17Integer
227Spectra Counts [2] [Dpp9] (1μM**)Number of spectra counts for Dpp9 in test compound treated sample, replicate 2.17Integer
228Spectra Counts [3] [Dpp9] (1μM**)Number of spectra counts for Dpp9 in test compound treated sample, replicate 3.17Integer
229Spectra Counts [4] [Dpp9] (1μM**)Number of spectra counts for Dpp9 in test compound treated sample, replicate 4.17Integer
230Average Spectra Counts [Dpp9] (1μM**)Average number of spectra counts for Dpp9 in test compound treated samples.17Integer
231Standard Deviation [Dpp9]Standard Deviation for the average number of spectra counts for Dpp9 in test compound treated samples.17Float
232Spectra Counts [1] [DMSO control, Dpp9]Number of spectra counts for Dpp9 in DMSO control sample, replicate 1.17Integer
233Spectra Counts [2] [DMSO control, Dpp9]Number of spectra counts for Dpp9 in DMSO control sample, replicate 2.17Integer
234Spectra Counts [3] [DMSO control, Dpp9]Number of spectra counts for Dpp9 in DMSO control sample, replicate 3.17Integer
235Spectra Counts [4] [DMSO control, Dpp9]Number of spectra counts for Dpp9 in DMSO control sample, replicate 4.17Integer
236Average Spectra Counts [DMSO control, Dpp9]Average number of spectra counts for Dpp9 in DMSO control samples.17Integer
237Standard Deviation [DMSO control, Dpp9]Standard deviation for the average number of spectra counts for Dpp9 in DMSO control samples.17Float
238Ratio Average Spectra Counts [Dpp9]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.17Floatratio
239Outcome [Dpp8]One of Active, Inactive, or Not Tested.18dipeptidyl peptidase 8 [Mus musculus]Outcome
240Spectra Counts [1] [Dpp8] (1μM**)Number of spectra counts for Dpp8 in test compound treated sample, replicate 1.18Integer
241Spectra Counts [2] [Dpp8] (1μM**)Number of spectra counts for Dpp8 in test compound treated sample, replicate 2.18Integer
242Spectra Counts [3] [Dpp8] (1μM**)Number of spectra counts for Dpp8 in test compound treated sample, replicate 3.18Integer
243Spectra Counts [4] [Dpp8] (1μM**)Number of spectra counts for Dpp8 in test compound treated sample, replicate 4.18Integer
244Average Spectra Counts [Dpp8] (1μM**)Average number of spectra counts for Dpp8 in test compound treated samples.18Integer
245Standard Deviation [Dpp8]Standard Deviation for the average number of spectra counts for Dpp8 in test compound treated samples.18Float
246Spectra Counts [1] [DMSO control, Dpp8]Number of spectra counts for Dpp8 in DMSO control sample, replicate 1.18Integer
247Spectra Counts [2] [DMSO control, Dpp8]Number of spectra counts for Dpp8 in DMSO control sample, replicate 2.18Integer
248Spectra Counts [3] [DMSO control, Dpp8]Number of spectra counts for Dpp8 in DMSO control sample, replicate 3.18Integer
249Spectra Counts [4] [DMSO control, Dpp8]Number of spectra counts for Dpp8 in DMSO control sample, replicate 4.18Integer
250Average Spectra Counts [DMSO control, Dpp8]Average number of spectra counts for Dpp8 in DMSO control samples.18Integer
251Standard Deviation [DMSO control, Dpp8]Standard deviation for the average number of spectra counts for Dpp8 in DMSO control samples.18Float
252Ratio Average Spectra Counts [Dpp8]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.18Floatratio
253Outcome [Pafah1b3]One of Active, Inactive, or Not Tested.19platelet-activating factor acetylhydrolase IB subunit gamma [Mus musculus]Outcome
254Spectra Counts [1] [Pafah1b3] (1μM**)Number of spectra counts for Pafah1b3 in test compound treated sample, replicate 1.19Integer
255Spectra Counts [2] [Pafah1b3] (1μM**)Number of spectra counts for Pafah1b3 in test compound treated sample, replicate 2.19Integer
256Spectra Counts [3] [Pafah1b3] (1μM**)Number of spectra counts for Pafah1b3 in test compound treated sample, replicate 3.19Integer
257Spectra Counts [4] [Pafah1b3] (1μM**)Number of spectra counts for Pafah1b3 in test compound treated sample, replicate 4.19Integer
258Average Spectra Counts [Pafah1b3] (1μM**)Average number of spectra counts for Pafah1b3 in test compound treated samples.19Integer
259Standard Deviation [Pafah1b3]Standard Deviation for the average number of spectra counts for Pafah1b3 in test compound treated samples.19Float
260Spectra Counts [1] [DMSO control, Pafah1b3]Number of spectra counts for Pafah1b3 in DMSO control sample, replicate 1.19Integer
261Spectra Counts [2] [DMSO control, Pafah1b3]Number of spectra counts for Pafah1b3 in DMSO control sample, replicate 2.19Integer
262Spectra Counts [3] [DMSO control, Pafah1b3]Number of spectra counts for Pafah1b3 in DMSO control sample, replicate 3.19Integer
263Spectra Counts [4] [DMSO control, Pafah1b3]Number of spectra counts for Pafah1b3 in DMSO control sample, replicate 4.19Integer
264Average Spectra Counts [DMSO control, Pafah1b3]Average number of spectra counts for Pafah1b3 in DMSO control samples.19Integer
265Standard Deviation [DMSO control, Pafah1b3]Standard deviation for the average number of spectra counts for Pafah1b3 in DMSO control samples.19Float
266Ratio Average Spectra Counts [Pafah1b3]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.19Floatratio
267Outcome [Magl]One of Active, Inactive, or Not Tested.20monoglyceride lipase isoform d [Mus musculus]Outcome
268Spectra Counts [1] [Magl] (1μM**)Number of spectra counts for Magl in test compound treated sample, replicate 1.20Integer
269Spectra Counts [2] [Magl] (1μM**)Number of spectra counts for Magl in test compound treated sample, replicate 2.20Integer
270Spectra Counts [3] [Magl] (1μM**)Number of spectra counts for Magl in test compound treated sample, replicate 3.20Integer
271Spectra Counts [4] [Magl] (1μM**)Number of spectra counts for Magl in test compound treated sample, replicate 4.20Integer
272Average Spectra Counts [Magl] (1μM**)Average number of spectra counts for Magl in test compound treated samples.20Integer
273Standard Deviation [Magl]Standard Deviation for the average number of spectra counts for Magl in test compound treated samples.20Float
274Spectra Counts [1] [DMSO control, Magl]Number of spectra counts for Magl in DMSO control sample, replicate 1.20Integer
275Spectra Counts [2] [DMSO control, Magl]Number of spectra counts for Magl in DMSO control sample, replicate 2.20Integer
276Spectra Counts [3] [DMSO control, Magl]Number of spectra counts for Magl in DMSO control sample, replicate 3.20Integer
277Spectra Counts [4] [DMSO control, Magl]Number of spectra counts for Magl in DMSO control sample, replicate 4.20Integer
278Average Spectra Counts [DMSO control, Magl]Average number of spectra counts for Magl in DMSO control samples.20Integer
279Standard Deviation [DMSO control, Magl]Standard deviation for the average number of spectra counts for Magl in DMSO control samples.20Float
280Ratio Average Spectra Counts [Magl]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.20Floatratio
281Outcome [Abdh4]One of Active, Inactive, or Not Tested.21abhydrolase domain-containing protein 4 isoform 1 [Mus musculus]Outcome
282Spectra Counts [1] [Abdh4] (1μM**)Number of spectra counts for Abhd4 in test compound treated sample, replicate 1.21Integer
283Spectra Counts [2] [Abdh4] (1μM**)Number of spectra counts for Abhd4 in test compound treated sample, replicate 2.21Integer
284Spectra Counts [3] [Abdh4] (1μM**)Number of spectra counts for Abhd4 in test compound treated sample, replicate 3.21Integer
285Spectra Counts [4] [Abdh4] (1μM**)Number of spectra counts for Abhd4 in test compound treated sample, replicate 4.21Integer
286Average Spectra Counts [Abdh4] (1μM**)Average number of spectra counts for Abhd4 in test compound treated samples.21Integer
287Standard Deviation [Abdh4]Standard Deviation for the average number of spectra counts for Abhd4 in test compound treated samples.21Float
288Spectra Counts [1] [DMSO control, Abdh4]Number of spectra counts for Abhd4 in DMSO control sample, replicate 1.21Integer
289Spectra Counts [2] [DMSO control, Abdh4]Number of spectra counts for Abhd4 in DMSO control sample, replicate 2.21Integer
290Spectra Counts [3] [DMSO control, Abdh4]Number of spectra counts for Abhd4 in DMSO control sample, replicate 3.21Integer
291Spectra Counts [4] [DMSO control, Abdh4]Number of spectra counts for Abhd4 in DMSO control sample, replicate 4.21Integer
292Average Spectra Counts [DMSO control, Abdh4]Average number of spectra counts for Abhd4 in DMSO control samples.21Integer
293Standard Deviation [DMSO control, Abdh4]Standard deviation for the average number of spectra counts for Abhd4 in DMSO control samples.21Float
294Ratio Average Spectra Counts [Abdh4]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.21Floatratio
295Outcome [Abdh11]One of Active, Inactive, or Not Tested.22abhydrolase domain-containing protein 11 isoform 1 [Mus musculus]Outcome
296Spectra Counts [1] [Abdh11] (1μM**)Number of spectra counts for Abhd11 in test compound treated sample, replicate 1.22Integer
297Spectra Counts [2] [Abdh11] (1μM**)Number of spectra counts for Abhd11 in test compound treated sample, replicate 2.22Integer
298Spectra Counts [3] [Abdh11] (1μM**)Number of spectra counts for Abhd11 in test compound treated sample, replicate 3.22Integer
299Spectra Counts [4] [Abdh11] (1μM**)Number of spectra counts for Abhd11 in test compound treated sample, replicate 4.22Integer
300Average Spectra Counts [Abdh11] (1μM**)Average number of spectra counts for Abhd11 in test compound treated samples.22Integer
301Standard Deviation [Abdh11]Standard Deviation for the average number of spectra counts for Abhd11 in test compound treated samples.22Float
302Spectra Counts [1] [DMSO control, Abdh11]Number of spectra counts for Abhd11 in DMSO control sample, replicate 1.22Integer
303Spectra Counts [2] [DMSO control, Abdh11]Number of spectra counts for Abhd11 in DMSO control sample, replicate 2.22Integer
304Spectra Counts [3] [DMSO control, Abdh11]Number of spectra counts for Abhd11 in DMSO control sample, replicate 3.22Integer
305Spectra Counts [4] [DMSO control, Abdh11]Number of spectra counts for Abhd11 in DMSO control sample, replicate 4.22Integer
306Average Spectra Counts [DMSO control, Abdh11]Average number of spectra counts for Abhd11 in DMSO control samples.22Integer
307Standard Deviation [DMSO control, Abdh11]Standard deviation for the average number of spectra counts for Abhd11 in DMSO control samples.22Float
308Ratio Average Spectra Counts [Abdh11]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.22Floatratio
309Outcome [Pla2g15]One of Active, Inactive, or Not Tested.23group XV phospholipase A2 precursor [Mus musculus]Outcome
310Spectra Counts [1] [Pla2g15] (1μM**)Number of spectra counts for Pla2g15 in test compound treated sample, replicate 1.23Integer
311Spectra Counts [2] [Pla2g15] (1μM**)Number of spectra counts for Pla2g15 in test compound treated sample, replicate 2.23Integer
312Spectra Counts [3] [Pla2g15] (1μM**)Number of spectra counts for Pla2g15 in test compound treated sample, replicate 3.23Integer
313Spectra Counts [4] [Pla2g15] (1μM**)Number of spectra counts for Pla2g15 in test compound treated sample, replicate 4.23Integer
314Average Spectra Counts [Pla2g15] (1μM**)Average number of spectra counts for Pla2g15 in test compound treated samples.23Integer
315Standard Deviation [Pla2g15]Standard Deviation for the average number of spectra counts for Pla2g15 in test compound treated samples.23Float
316Spectra Counts [1] [DMSO control, Pla2g15]Number of spectra counts for Pla2g15 in DMSO control sample, replicate 1.23Integer
317Spectra Counts [2] [DMSO control, Pla2g15]Number of spectra counts for Pla2g15 in DMSO control sample, replicate 2.23Integer
318Spectra Counts [3] [DMSO control, Pla2g15]Number of spectra counts for Pla2g15 in DMSO control sample, replicate 3.23Integer
319Spectra Counts [4] [DMSO control, Pla2g15]Number of spectra counts for Pla2g15 in DMSO control sample, replicate 4.23Integer
320Average Spectra Counts [DMSO control, Pla2g15]Average number of spectra counts for Pla2g15 in DMSO control samples.23Integer
321Standard Deviation [DMSO control, Pla2g15]Standard deviation for the average number of spectra counts for Pla2g15 in DMSO control samples.23Float
322Ratio Average Spectra Counts [Pla2g15]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.23Floatratio
323Outcome [Lyplal1]One of Active, Inactive, or Not Tested.24lysophospholipase-like protein 1 [Mus musculus]Outcome
324Spectra Counts [1] [Lyplal1] (1μM**)Number of spectra counts for Lyplal1 in test compound treated sample, replicate 1.24Integer
325Spectra Counts [2] [Lyplal1] (1μM**)Number of spectra counts for Lyplal1 in test compound treated sample, replicate 2.24Integer
326Spectra Counts [3] [Lyplal1] (1μM**)Number of spectra counts for Lyplal1 in test compound treated sample, replicate 3.24Integer
327Spectra Counts [4] [Lyplal1] (1μM**)Number of spectra counts for Lyplal1 in test compound treated sample, replicate 4.24Integer
328Average Spectra Counts [Lyplal1] (1μM**)Average number of spectra counts for Lyplal1 in test compound treated samples.24Integer
329Standard Deviation [Lyplal1]Standard Deviation for the average number of spectra counts for Lyplal1 in test compound treated samples.24Float
330Spectra Counts [1] [DMSO control, Lyplal1]Number of spectra counts for Lyplal1 in DMSO control sample, replicate 1.24Integer
331Spectra Counts [2] [DMSO control, Lyplal1]Number of spectra counts for Lyplal1 in DMSO control sample, replicate 2.24Integer
332Spectra Counts [3] [DMSO control, Lyplal1]Number of spectra counts for Lyplal1 in DMSO control sample, replicate 3.24Integer
333Spectra Counts [4] [DMSO control, Lyplal1]Number of spectra counts for Lyplal1 in DMSO control sample, replicate 4.24Integer
334Average Spectra Counts [DMSO control, Lyplal1]Average number of spectra counts for Lyplal1 in DMSO control samples.24Integer
335Standard Deviation [DMSO control, Lyplal1]Standard deviation for the average number of spectra counts for Lyplal1 in DMSO control samples.24Float
336Ratio Average Spectra Counts [Lyplal1]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.24Floatratio
337Outcome [Lipe]One of Active, Inactive, or Not Tested.25hormone-sensitive lipase isoform 2 [Mus musculus]Outcome
338Spectra Counts [1] [Lipe] (1μM**)Number of spectra counts for Lipe in test compound treated sample, replicate 1.25Integer
339Spectra Counts [2] [Lipe] (1μM**)Number of spectra counts for Lipe in test compound treated sample, replicate 2.25Integer
340Spectra Counts [3] [Lipe] (1μM**)Number of spectra counts for Lipe in test compound treated sample, replicate 3.25Integer
341Spectra Counts [4] [Lipe] (1μM**)Number of spectra counts for Lipe in test compound treated sample, replicate 4.25Integer
342Average Spectra Counts [Lipe] (1μM**)Average number of spectra counts for Lipe in test compound treated samples.25Integer
343Standard Deviation [Lipe]Standard Deviation for the average number of spectra counts for Lipe in test compound treated samples.25Float
344Spectra Counts [1] [DMSO control, Lipe]Number of spectra counts for Lipe in DMSO control sample, replicate 1.25Integer
345Spectra Counts [2] [DMSO control, Lipe]Number of spectra counts for Lipe in DMSO control sample, replicate 2.25Integer
346Spectra Counts [3] [DMSO control, Lipe]Number of spectra counts for Lipe in DMSO control sample, replicate 3.25Integer
347Spectra Counts [4] [DMSO control, Lipe]Number of spectra counts for Lipe in DMSO control sample, replicate 4.25Integer
348Average Spectra Counts [DMSO control, Lipe]Average number of spectra counts for Lipe in DMSO control samples.25Integer
349Standard Deviation [DMSO control, Lipe]Standard deviation for the average number of spectra counts for Lipe in DMSO control samples.25Float
350Ratio Average Spectra Counts [Lipe]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.25Floatratio
351Outcome [Tpp2]One of Active, Inactive, or Not Tested.26Tpp2 protein [Mus musculus]Outcome
352Spectra Counts [1] [Tpp2] (1μM**)Number of spectra counts for Tpp2 in test compound treated sample, replicate 1.26Integer
353Spectra Counts [2] [Tpp2] (1μM**)Number of spectra counts for Tpp2 in test compound treated sample, replicate 2.26Integer
354Spectra Counts [3] [Tpp2] (1μM**)Number of spectra counts for Tpp2 in test compound treated sample, replicate 3.26Integer
355Spectra Counts [4] [Tpp2] (1μM**)Number of spectra counts for Tpp2 in test compound treated sample, replicate 4.26Integer
356Average Spectra Counts [Tpp2] (1μM**)Average number of spectra counts for Tpp2 in test compound treated samples.26Integer
357Standard Deviation [Tpp2]Standard Deviation for the average number of spectra counts for Tpp2 in test compound treated samples.26Float
358Spectra Counts [1] [DMSO control, Tpp2]Number of spectra counts for Tpp2 in DMSO control sample, replicate 1.26Integer
359Spectra Counts [2] [DMSO control, Tpp2]Number of spectra counts for Tpp2 in DMSO control sample, replicate 2.26Integer
360Spectra Counts [3] [DMSO control, Tpp2]Number of spectra counts for Tpp2 in DMSO control sample, replicate 3.26Integer
361Spectra Counts [4] [DMSO control, Tpp2]Number of spectra counts for Tpp2 in DMSO control sample, replicate 4.26Integer
362Average Spectra Counts [DMSO control, Tpp2]Average number of spectra counts for Tpp2 in DMSO control samples.26Integer
363Standard Deviation [DMSO control, Tpp2]Standard deviation for the average number of spectra counts for Tpp2 in DMSO control samples.26Float
364Ratio Average Spectra Counts [Tpp2]Ratio of average number of spectra counts in test compound treated samples over DMSO control samples.26Floatratio

** Test Concentration. § Panel component ID.
Additional Information
Grant Number: 1R01HL084366

Classification
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