AID	Name	Type	Comment
588579	qHTS for Inhibitors of Polymerase Kappa	confirmatory	qHTS

The processing and repair of DNA interstrand crosslinks (ICL) in eukaryotic cells are extremely complex, potentially involving multiple DNA repair and damage tolerance pathways, including homologous recombination, nucleotide excision repair, translesion synthesis (TLS), and cell cycle checkpoints. In some repair models, the dually-incised strand possesses sufficient mobility that a DNA polymerase can strand displace the nucleotide patch that is 5' to the lesion, then replicate past the ICL site to complete the repair gap-filling synthesis. Polymerase kappa has been implicated in this project and is hence of interest.

To that end, in a collaboration between the Oregon Health & Science University and the NIH Chemical Genomics Center a 1536-well high-throughput screen was developed. This fluorescent screen was assayed against the NIH Molecular Libraries Small Molecule Repository (MLSMR) to discover inhibitors of Pol Kappa.


NIH Chemical Genomics Center [NCGC]
NIH Molecular Libraries Probe Centers Network [MLPCN]

MLPCN Grant: MH094179
Assay Submitter (PI): Stephen Lloyd, Oregon Health & Science University

Please see linked AIDs for detailed descriptions of each assay.

This project is on-going and will be updated at a later point with our findings.

Grant Number: MH094179