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BioAssay: AID 51033

Concentration required for obtaining 50% of the fluorescence intensity of copper-free calcein 1, in bovine serum albumin

The water soluble fluorescein-based ligand 1 forms a non-fluorescent complex with Cu(2+). This complex serves as a fluorescent sensor for amino acids in the 10(-3) M concentration range. Since the signal response is very fast, the sensor can be used to detect the hydrolytic activity of various proteases (trypsin, chymotrypsin, subtilisin) on bovine serum albumin as a whole protein substrate, and more generally to follow reactions releasing or removing free amino acids, in real time. ..more
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 Tested Compounds
 Tested Compounds
All(11)
 
 
Active(3)
 
 
Unspecified(8)
 
 
 Tested Substances
 Tested Substances
All(11)
 
 
Active(3)
 
 
Unspecified(8)
 
 
 Related BioAssays
 Related BioAssays
AID: 51033
Data Source: ChEMBL (48177)
Depositor Category: Literature, Extracted
BioAssay Version:
Deposit Date: 2010-05-20
Modify Date: 2013-11-12

Data Table ( Complete ):           Active    All
BioActive Compounds: 3
Description:
Title: A green fluorescent chemosensor for amino acids provides a versatile high-throughput screening (HTS) assay for proteases.

Abstract: The water soluble fluorescein-based ligand 1 forms a non-fluorescent complex with Cu(2+). This complex serves as a fluorescent sensor for amino acids in the 10(-3) M concentration range. Since the signal response is very fast, the sensor can be used to detect the hydrolytic activity of various proteases (trypsin, chymotrypsin, subtilisin) on bovine serum albumin as a whole protein substrate, and more generally to follow reactions releasing or removing free amino acids, in real time.
(PMID: 12729634)
Comment
Compounds with activity <= 50uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Putative Target:

ChEMBL Target ID: 22226
Target Type: UNCHECKED
Pref Name: Unchecked
Confidence: Default value - Target unknown or has yet to be assigned
Relationship Type: Default value - Target has yet to be curated
Categorized Comment
ChEMBL Assay Type: Binding

ChEMBL Assay Data Source: Scientific Literature

Result Definitions
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
1EC50*EC50 PubChem standard valueFloatμM
2EC50 activity commentEC50 activity commentString
3EC50 standard flagEC50 standard flagInteger
4EC50 qualifierEC50 qualifierString
5EC50 published valueEC50 published valueFloatmM
6EC50 standard valueEC50 standard valueFloatnM
7EC50 data validityEC50 data validityString

* Activity Concentration.

Data Table (Concise)
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