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BioAssay: AID 504579

Inhibitors of T-Type Calcium Channels (rat DRG neuron currents)

T-type Ca2+ channels are also called low voltage-activated channels because they open at voltages near the resting membrane potential of most cells. In many types of neurons, Ca2+ influx through T-type channels triggers low-threshold spikes, which in turn trigger a burst of action potentials mediated by Na+ channels (1). Burst firing is thought to play an important role in the synchronized more ..
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AID: 504579
Data Source: Vanderbilt Screening Center for GPCRs, Ion Channels and Transporters (Ttype DRG neuron currents (rat))
BioAssay Type: Panel
Depositor Category: NIH Molecular Libraries Screening Center Network
Deposit Date: 2011-03-25

Data Table ( Complete ):           View Active Data    View All Data
Target
BioActive Compound: 1
Related Experiments
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AIDNameTypeComment
449739Inhibitors of Cav3 T-type Calcium Channels: Primary ScreenScreeningdepositor-specified cross reference
463087Inhibitors of Cav3 T-type Calcium ChannelsSummarydepositor-specified cross reference
489005Inhibitors of T-Type Calcium ChannelConfirmatorydepositor-specified cross reference
493021Inhibitors of T-Type Calcium ChannelsConfirmatorydepositor-specified cross reference
493022Inhibitors of T-Type Calcium Channels (SynthLib1)Confirmatorydepositor-specified cross reference
493023Inhibitors of T-Type Calcium Channels (SynthLib2)Confirmatorydepositor-specified cross reference
493041Inhibitors of T-Type Calcium Channels (SynthLib3)Confirmatorydepositor-specified cross reference
504425Mode of action assay-Specificity dose response assay for the identification of selective inhibitors of T-type calcium subunit Cav3.2 in the Cav3.3 expressing cell line on automated patch clampConfirmatorydepositor-specified cross reference
504426Mode of action assay-Dose response assay for compounds that inhibit T-type calcium channel subunit Cav3.2 on automated patch clampConfirmatorydepositor-specified cross reference
504619Inhibitors of T-Type Calcium Channels (Cav3.2 HEK whole cell CRC)Confirmatorydepositor-specified cross reference
504628Inhibitors of T-Type Calcium Channels (Cav3.2 HEK whole cell)Otherdepositor-specified cross reference
504584Inhibitors of T-Type Calcium Channels (Ancillary Pharm)Othersame project related to Summary assay
Description:
Assay Provider: Xinmin Xie
Assay Provider Affiliation: Bioscience Division, SRI International, Menlo Park, CA
Grant Title: HTS Assay for Cav3 T-Type Channels using FLIPR
Grant Number: NS050771-01

T-type Ca2+ channels are also called low voltage-activated channels because they open at voltages near the resting membrane potential of most cells. In many types of neurons, Ca2+ influx through T-type channels triggers low-threshold spikes, which in turn trigger a burst of action potentials mediated by Na+ channels (1). Burst firing is thought to play an important role in the synchronized activity of the thalamus observed in absence epilepsy, and also in a wider range of neurological disorders characterized by thalamocortical dysrhythmia (2). Prominent T-currents are also observed in dorsal root ganglion neurons, with subsets of nociceptors expressing more T-current than high voltage-activated Ca2+ currents (3). Considerable evidence supports the notion that a T-channel antagonist would be a useful drug for the treatment of pain and epilepsy (4).

1. Perez-Reyes, E: Molecular physiology of low-voltage-activated T-type calcium channels. Physiol. Rev. 2003; 83: 117-161.
2. Llinas, R R, Ribary, U, Jeanmonod, D, Kronberg, E, and Mitra, P P: Thalamocortical dysrhythmia: A neurological and neuropsychiatric syndrome characterized by magnetoencephalography. Proc. Natl. Acad. Sci. U.S.A. 1999; 96: 15222-15227.
3. Nelson, M T, Joksovic, P M, Perez-Reyes, E, and Todorovic, S M: The endogenous redox agent L-cysteine induces T-type Ca2+ channel-dependent sensitization of a novel subpopulation of rat peripheral nociceptors. J. Neurosci. 2005; 25: 8766-8775.
4. Nelson, M, Todorovic, S, and Perez-Reyes, E: The role of T-type calcium channels in epilepsy and pain. Curr Pharm Des 2006; 12: 2189-2197.
Panel Information
Inhibitors of T-Type Calcium Channels (DRG neuron current Ttype, Na+, K+ currents) - Measurement of ML218 inhibition of rat DRG neuron currents with whole cell voltage clamp
PID§NameSubstancePanel TargetsDescriptionAdditional Information
ActiveInactive
1DRG T-type currentWhole cell voltage clamp in native Dorsal Root Ganglion (DRG) neurons to determine effects on endogenous T-type currentsTaxonomy id: 10116
2DRG Na+ currentWhole cell voltage clamp in native Dorsal Root Ganglion (DRG) neurons to determine effects on endogenous voltage-gated TTX-sensitive Na+ currentsTaxonomy id: 10116
3DRG K+ currentWhole cell voltage clamp in native Dorsal Root Ganglion (DRG) neurons to determine effects on endogenous voltage-gated K+ currentsTaxonomy id: 10116

§ Panel component ID.
Protocol
The purpose of this assay was to follow up on the confirmatory and dose-response testing of the validated lead SAR series to characterize the candidate probe compound for in native rat Dorsal Root Ganglion (DRG) neurons to determine effects on endogenous T-type, Na+ and K+ currents.
Whole-cell voltage-clamp recording techniques were employed. Ba2+ (10 mM, replacement of Ca2+) was used as the charge carrier to generate larger and relatively more stable currents. For recording T-channels, Ba2+ currents were rapidly activated and inactivated following step depolarization to various test potentials from a holding potential (Vh) of -100 mV. Then, for a single pulse, depolarized to -20mV. For recording voltage-gated TTX-sensitive Na channels, Na+ currents were activated following step depolarization to various test potentials or single pulse to +10mV from Vh of -100 mV. For recording voltage-gated K channels, K+ currents were activated following step depolarization to various test potentials or single pulse to +70mV from Vh of -60 mV.
Result Definitions
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TIDNameDescriptionPID§Panel TargetsHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1Time_0Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
2Time_5Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
3Time_10Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
4Time_15Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
5Time_20Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
6Time_25Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
7Time_30Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
8Time_35Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
9Time_40Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
10Time_45Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
11Time_50Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
12Time_55Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
13Time_60Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
14Time_65Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
15Time_70Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
16Time_75Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
17Time_80Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
18Time_85Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
19Time_90Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
20Time_95Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
21Time_100Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
22Time_105Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
23Time_110Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
24Time_115Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
25Time_120Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
26Time_125Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
27Time_130Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
28Time_135Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
29Time_140Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
30Time_145Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
31Time_150Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
32Time_155Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
33Time_160Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
34Time_165Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
35Time_170Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
36Time_175Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
37Time_180Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
38Time_185Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
39Time_190Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
40Time_195Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
41Time_200Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
42Time_205Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
43Time_210Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
44Time_215Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
45Time_220Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
46Time_225Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
47Time_230Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
48Time_235Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
49Time_240Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
50Time_245Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
51Time_250Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
52Time_255Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
53Time_260Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
54Time_265Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
55Time_270Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
56Time_275Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
57Time_280Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
58Time_285Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
59Time_290Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
60Time_295Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
61Time_300Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
62Time_305Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
63Time_310Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
64Time_315Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
65Time_320Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
66Time_325Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
67Time_330Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
68Time_335Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
69Time_340Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
70Time_345Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
71Time_350Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
72Time_355Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
73Time_360Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
74Time_365Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
75Time_370Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
76Time_375Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
77Time_380Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
78Time_385Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
79Time_390Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
80Time_395Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
81Time_400Current in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).1FloatUnspecify
82Time_0_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
83Time_5_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
84Time_10_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
85Time_15_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
86Time_20_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
87Time_25_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
88Time_30_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
89Time_35_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
90Time_40_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
91Time_45_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
92Time_50_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
93Time_55_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
94Time_60_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
95Time_65_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
96Time_70_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
97Time_75_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
98Time_80_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
99Time_85_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
100Time_90_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
101Time_95_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
102Time_100_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
103Time_105_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
104Time_110_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
105Time_115_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
106Time_120_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
107Time_125_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
108Time_130_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
109Time_135_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
110Time_140_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
111Time_145_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
112Time_150_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
113Time_155_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
114Time_160_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
115Time_165_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
116Time_170_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
117Time_175_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
118Time_180_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
119Time_185_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
120Time_190_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
121Time_195_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
122Time_200_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
123Time_205_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
124Time_210_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
125Time_215_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
126Time_220_NaCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).2FloatUnspecify
127Time_0_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
128Time_5_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
129Time_10_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
130Time_15_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
131Time_20_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
132Time_25_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
133Time_30_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
134Time_35_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
135Time_40_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
136Time_45_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
137Time_50_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
138Time_55_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
139Time_60_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
140Time_65_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
141Time_70_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
142Time_75_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
143Time_80_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
144Time_85_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
145Time_90_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
146Time_95_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
147Time_100_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
148Time_105_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
149Time_110_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
150Time_115_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
151Time_120_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
152Time_125_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
153Time_130_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
154Time_135_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
155Time_140_KCurrent in Dorsal Root Ganglion (DRG) neurons recorded in 5 second intervals using a single voltage step from either -100 or -60 mV and depolarizing to +20mV. Test compound concentration at 1uM. Current reported in picoamps (pA).3FloatUnspecify
Additional Information
Grant Number: NS050771-01

Classification
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