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BioAssay: AID 492140

Antioxidant activity assessed as formazan formation induced absorbance changes at 25 ppm at 570 nm at 37 degC for 6 hrs by MTT assay

Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected more ..
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 Tested Compounds
 Tested Compounds
All(76)
 
 
Unspecified(76)
 
 
 Tested Substances
 Tested Substances
All(77)
 
 
Unspecified(77)
 
 
AID: 492140
Data Source: ChEMBL (642547)
Depositor Category: Literature, Extracted
BioAssay Version:
Deposit Date: 2010-12-02
Modify Date: 2014-05-25

Data Table ( Complete ):           View All Data
Tested Compounds:
Description:
Title: An efficient and economical MTT assay for determining the antioxidant activity of plant natural product extracts and pure compounds.

Abstract: Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected natural product extracts and purified compounds. This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput.
(PMID: 20565070)
Comment
Putative Target:
ChEMBL Target ID: 22226
Target Type: UNCHECKED
Pref Name: Unchecked
Confidence: Default value - Target unknown or has yet to be assigned
Relationship Type: Default value - Target has yet to be curated
Categorized Comment - additional comments and annotations
From ChEMBL:
Assay Type: Functional
Assay Data Source: Scientific Literature
Result Definitions
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
1deltaA activity commentdeltaA activity commentString
2deltaA standard flagdeltaA standard flagInteger
3deltaA qualifierdeltaA qualifierString
4deltaA published valuedeltaA published valueFloat
5deltaA standard valuedeltaA standard valueFloat

Data Table (Concise)
Data Table ( Complete ):     View All Data
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