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BioAssay: AID 488831

SAR Analysis of Antagonists of the DOR Receptor using an Image-Based Assay - Set 3

Drug addiction is a disease that involves the G-protein coupled receptors in the central nervous system resulting in compulsive or abnormal behavior. Recent studies have shown that opioid receptors play a role in regulating other receptors that interact with drug and other substance abuse. The opioid receptors are composed of multiple of multiple receptor subtypes whose contribution the addictive behaviors are not fully delineated. ..more
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AID: 488831
Data Source: Burnham Center for Chemical Genomics (SBCCG-A464-DOR-Antagonist-DryPowder-Assay-3)
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Depositor Category: NIH Molecular Libraries Probe Production Network
BioAssay Version:
Deposit Date: 2010-10-19
Hold-until Date: 2011-05-01
Modify Date: 2011-05-03

Data Table ( Complete ):           View All Data
Target
Tested Compounds:
Related Experiments
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AIDNameTypeProbeComment
1777uHTS identification of small molecule agonists of the kappa opioid receptor via a luminescent beta-arrestin assayConfirmatory depositor-specified cross reference
1785Summary of small molecule antagonists of the kappa opioid receptorSummary2 depositor-specified cross reference: Summary AID.
1778uHTS identification of small molecule antagonists of the kappa opioid receptor via a luminescent beta-arrestin assayConfirmatory same project related to Summary assay
1966HTS Dose response counterscreen for assays utilizing the enzyme, b-galactosidaseConfirmatory same project related to Summary assay
2136HTS Image-Based Screen for Selective Antagonists of the KOR ReceptorConfirmatory same project related to Summary assay
2285SAR analysis of small molecule antagonists of the kappa opioid receptor via a luminescent beta-arrestin assayConfirmatory same project related to Summary assay
2344HTS Image-Based Screen for Agonists of the MOR ReceptorConfirmatory same project related to Summary assay
2348SAR analysis of Antagonists of the Kappa Opioid Receptor (KOR) using an Image-Based AssayConfirmatory same project related to Summary assay
2352SAR Analysis of Agonists of the MOR Receptor using an Image-Based AssayConfirmatory same project related to Summary assay
2356HTS Image-Based Screen for Antagonists of the DOR ReceptorConfirmatory same project related to Summary assay
2357SAR Analysis of Antagonists of the DOR Receptor using an Image-Based AssayConfirmatory same project related to Summary assay
2359SAR analysis of Agonists of the Kappa Opioid Receptor (KOR) using an Image-Based AssayConfirmatory same project related to Summary assay
2370SAR Analysis of Agonists of the DOR Receptor using an Image-Based AssayConfirmatory same project related to Summary assay
2420SAR Analysis of Antagonists of the MOR Receptor using an Image-Based AssayConfirmatory same project related to Summary assay
2478SAR Analysis of Antagonists of the MOR Receptor using an Image-Based Assay - Set 2Confirmatory same project related to Summary assay
2491SAR analysis of Antagonists of the Kappa Opioid Receptor (KOR) using an Image-Based Assay - Set 2Confirmatory same project related to Summary assay
2492SAR Analysis of Agonists of the MOR Receptor using an Image-Based Assay - Set 2Confirmatory same project related to Summary assay
2493SAR Analysis of Agonists of the DOR Receptor using an Image-Based Assay - Set 2Confirmatory same project related to Summary assay
2495SAR analysis of small molecule antagonists of the kappa opioid receptor via a luminescent beta-arrestin assay - Set 2Confirmatory same project related to Summary assay
2498SAR Analysis of Antagonists of the DOR Receptor using an Image-Based Assay - Set 2Confirmatory same project related to Summary assay
2500SAR analysis of small molecule agonists of the kappa opioid receptor via a luminescent beta-arrestin assay - Set 2Confirmatory same project related to Summary assay
434981SAR analysis of small molecule antagonists of the kappa opioid receptor via a luminescent beta-arrestin assay - Set 3Confirmatory same project related to Summary assay
449737SAR analysis of small molecule agonists of the kappa opioid receptor via a luminescent beta-arrestin assay - Set 3Confirmatory same project related to Summary assay
463105SAR analysis of small molecule antagonists of the kappa opioid receptor via a luminescent beta-arrestin assay - Set 4Confirmatory same project related to Summary assay
488819SAR analysis of Antagonists of the Kappa Opioid Receptor (KOR) using an Image-Based Assay - Set 3Confirmatory same project related to Summary assay
488822SAR Analysis of Agonists of the MOR Receptor using an Image-Based Assay - Set 3Confirmatory same project related to Summary assay
488826SAR analysis of small molecule antagonists of the kappa opioid receptor via a luminescent beta-arrestin assay - Set 5Confirmatory same project related to Summary assay
488842SAR Analysis of Antagonists of the MOR Receptor using an Image-Based Assay - Set 3Confirmatory same project related to Summary assay
488925SAR analysis of Antagonists of the Kappa Opioid Receptor (KOR) using an Image-Based Assay - Set 4Confirmatory same project related to Summary assay
488935SAR analysis of small molecule antagonists of the kappa opioid receptor via a luminescent beta-arrestin assay - Set 6Confirmatory same project related to Summary assay
Description:
Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG)
Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA)
Network: NIH Molecular Libraries Probe Production Centers Network(MLPCN)
Grant Number: 1X01DA026208-01
Assay Provider: Dr. Lawrance Barak, Duke University, Durham NC

Drug addiction is a disease that involves the G-protein coupled receptors in the central nervous system resulting in compulsive or abnormal behavior. Recent studies have shown that opioid receptors play a role in regulating other receptors that interact with drug and other substance abuse. The opioid receptors are composed of multiple of multiple receptor subtypes whose contribution the addictive behaviors are not fully delineated.

The aim of this assay is to identify small molecule antagonists of the human delta opioid receptor (DOR). This dose response assay is developed and performed to confirm hits originally identified in "uHTS identification of small molecule agonists of the kappa opioid receptor via a luminescent beta-arrestin assay" (AID 1777) and to study the structure-activity relationship on analogs of the confirmed hits. Compounds are either acquired from commercial sources or synthesized internally.

This image-based DOR agonist assay utilizes a cell line permanently expressing a beta-arrestin GFP biosensor and the delta opioid receptor (DOR). Upon agonist-mediated GPCR activation, the arrestin-GFP redistributes from the cytosolic compartment to the plasma membrane and further to coated pits / endosomal vesicles, which can be quantified as increased local aggregation of the GFP-arrestins.
Protocol
Assay Materials:
1) 384-well plates, black with clear bottom (Greiner# 781091)
2) U2OS (Human Osteosarcoma) cell line stably expressing the Beta-arrestin GFP and the DOR1 receptor
3) Culture Media: MEM with L-glutamine, Pen-strep, 10% Fetal Bovine Serum and selection antibiotics - 200 ug/ml G418 and 50 ug/ml Zeocin
4) Positive Control Working Solution: SNC80 (Tocris #0764-10 mg - 5 mM stock in DMSO) diluted in water to 1 uM.
5) Negative Control Working Solution: Naloxone hydrochloride dehydrate (Sigma N7758)- 5 mM stock diluted to 5 uM in water.
6) Test compounds from dry powder working solution: 10 mM in 100% DMSO
7) Fixative Working Solution: 6% Paraformaldehyde (PFA) diluted in PBS.
8) Nuclear Stain Working Solution: DAPI (Invitrogen, D1306) diluted to 150 ng/ml in DAPI buffer (10 mM TRIS, 10 mM EDTA, 100 mM NaCl, pH 7.4).

Assay Procedure:
1) 45 ul of cell suspension (200,000 cells/ml in culture medium) was dispensed in each well of the assay plates using a Wellmate bulk dispenser.
2) Plates are incubated overnight or approximately 20 hours at 37 degree C and 5% CO2.
3) Serum is removed by media aspiration and replaced with 45 ul serum-free MEM prior to addition of compounds.
4) Compound addition was done on the ECHO550 Liquid Handler. The "dose response protocol" was used to dispense corresponding volumes of each 10 mM compound on the assay plate.
a. Compounds were added to columns 3 to 22. Final concentration ranged from 62 nM to 32 uM (ten doses), in duplicate.
b. Positive control was added to column 1. Naloxone final concentration was 5 uM.
c. Negative control was added to column 2. DMSO was back-filled to each well to achieve a 0.31% final concentration.
5) Plates were incubated for 30 minutes at 37 degrees C and 5% CO2.
6) SNC80 was added to the entire plate except column 23. SNC80 final concentration was 100 nM. Plates were then incubated for another 45 minutes.
7) Media was aspirated leaving 20 ul liquid in each well using a Titertek plate washer.
8) 40 ul of fixative working solution was added to each well using a Wellmate bulk dispenser (Matrix) for a final concentration of 4% PFA.
9) Plates were incubated for 40 minutes at room temperature.
10) Fixative was aspirated and plates were washed twice with 50 ul PBS leaving 20 ul liquid in each well using a Titertek plate washer.
11) 40 ul of DAPI working solution was added using a Wellmate bulk dispenser for a final DAPI concentration of 100 ng/ml. Aluminum plate sealers were applied to each plate.

Image Acquisition and Analysis:
1) Image acquisition was performed on an Opera QEHS (Perkin Elmer) with 45 plate capacity loader/stacker and the following settings:
- 40x 0.6 NA air objective
- Acquisition camera set to 2-by-2 binning for an image size of 688 by 512 pixels
- 2 channels acquired sequentially: Exp1Cam1 = Beta-arrestin GFP using 488 nm laser excitation and 540/70 nm emission filters, Exp2Cam2 = DAPI (nuclei) using 365 nm Xenon lamp excitation and 450/50 nm emission filters
- 4 fields per well

2) Image analysis was performed using the Acapella Spot Detection Algorithm.

Analysis settings:
Nuclei Detection
Threshold Adjustment: 1.5
Nuclear Individual Threshold Adjustment: 0.45
Nuclear Splitting Adjustment: 7
Minimum Nuclear Area: 70
Minimum Nuclear Distance: 7
Minimum Nuclear Contrast: 0.1
Cytoplasm Detection
Cytoplasm Threshold Adjustment: 0.2
Cytoplasm Individual Threshold Adjustment: 0.1
Spot Detection
Spot Minimum Distance 3
Spot Peak Radius 0
Spot Reference Radius 3
Spot Minimum Contrast 0.3
Spot Minimum to Cell Intensity 1

Metrics calculated from
Nuclei Images: Cell Count (NumberofCellsAnalyzed), Nuclei Area (AreaoftheNucleus), Integrated Intensity of the Nuclei (TotalIntegratedIntensityoftheNucleus), Average Intensity of the Nuclei (AverageIntensityoftheNucleus)
GFP Images: Integrated Intensity of the Cytoplasm (TotalCytoplasmIntensity), Integrated Intensity of the Detected Spots (TotalSpotIntensity), Ratio of the Integrated Spot to Integrated Cytoplasm Intensities (RatioofSpotIntensitytoCytoplasmintensity), Number of Spots per Cell (AverageSpotsPerCell), Percentage of Cells Positive for Spot Formation (PercentagePositiveCells)

4) The "RatioofSpotIntensitytoCytoplasmintensity" metric was used to calculate the dose response curves and parameters.

IC50 values were calculated using CBIS software (ChemInnovations) employing a sigmoidal dose-response equation through non-linear regression.
Comment
Compounds with an IC50 < 10 uM are defined as actives in the dose response confirmation.

To simplify the distinction between the inactives of the primary screen and of the confirmatory screening stage, the Tiered Activity Scoring System was developed and implemented. Its utilization for the assay is described below.

Activity Scoring
Activity scoring rules were devised to take into consideration compound efficacy, its potential interference with the assay and the screening stage that the data was obtained. Details of the Scoring System will be published elsewhere. Briefly, the outline of the scoring system utilized for the assay is as follows:
1) First tier (0-40 range) is reserved for primary screening data and is not applicable to this assay

2) Second tier (41-80 range) is reserved for dose-response confirmation data and is not applicable to this assay

3) Third tier (81-100 range) is reserved for resynthesized true positives and their analogues.

a. Inactive compounds of the confirmatory stage are assigned a score value equal 81.
b. For the remaining compounds the score is linearly correlated with a compound's inhibitory potency and, in addition, provides a measure of the likelihood that the compound is not an artifact based on the available information.
c. Summary equation that takes into account the items discussed above is
Score = 82 + 3*(pIC50 - 3),
where pIC50 is a negative log(10) of the IC50 value expressed in mole/L concentration units. This equation results in the Score values above 85 for compounds that demonstrate high potency and predictable behavior. Compounds that are inactive in the assay or whose concentration-dependent behavior are likely to be an artifact of that assay will generally have lower Score values.
Categorized Comment - additional comments and annotations
From ChEMBL:
Assay Type: Functional
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1IC50_Mean_QualifierThis qualifier is to be used with the next TID, IC50_Mean. If qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
2IC50_Mean*IC50 value determined using sigmoidal dose response equationFloatμM
3IC50_Qualifier_1This qualifier is to be used with the next TID, IC50_1. If qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
4IC50_1IC50 value determined using sigmoidal dose response equationFloatμM
5Std.Err(IC50)_1Standard Error of IC50 valueFloatμM
6nH_1Hill coefficient determined using sigmoidal dose response equationFloat
7Excluded_Points_first_pointFlags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
8% Activity at 32 uM_first_point (32μM**)% Inhibition at a given concentrationFloat%
9% Activity at 16 uM_first_point (16μM**)% Inhibition at a given concentrationFloat%
10% Activity at 8 uM_first_point (8μM**)% Inhibition at a given concentrationFloat%
11% Activity at 4 uM_first_point (4μM**)% Inhibition at a given concentrationFloat%
12% Activity at 2 uM_first_point (2μM**)% Inhibition at a given concentrationFloat%
13% Activity at 1 uM_first_point (1μM**)% Inhibition at a given concentrationFloat%
14% Activity at 0.5 uM_first_point (0.5μM**)% Inhibition at a given concentrationFloat%
15% Activity at 0.25 uM_first_point (0.25μM**)% Inhibition at a given concentrationFloat%
16% Activity at 0.125 uM_first_point (0.125μM**)% Inhibition at a given concentrationFloat%
17% Activity at 0.0625 uM_first_point (0.0625μM**)% Inhibition at a given concentrationFloat%
18Excluded_Points_second_pointFlags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
19% Activity at 32 uM_second_point (32μM**)% Inhibition at a given concentrationFloat%
20% Activity at 16 uM_second_point (16μM**)% Inhibition at a given concentrationFloat%
21% Activity at 8 uM_second_point (8μM**)% Inhibition at a given concentrationFloat%
22% Activity at 4 uM_second_point (4μM**)% Inhibition at a given concentrationFloat%
23% Activity at 2 uM_second_point (2μM**)% Inhibition at a given concentrationFloat%
24% Activity at 1 uM_second_point (1μM**)% Inhibition at a given concentrationFloat%
25% Activity at 0.5 uM_second_point (0.5μM**)% Inhibition at a given concentrationFloat%
26% Activity at 0.25 uM_second_point (0.25μM**)% Inhibition at a given concentrationFloat%
27% Activity at 0.125 uM_second_point (0.125μM**)% Inhibition at a given concentrationFloat%
28% Activity at 0.0625 uM_second_point (0.0625μM**)% Inhibition at a given concentrationFloat%
29IC50_Qualifier_2This qualifier is to be used with the next TID, IC50_2. If qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
30IC50_2IC50 value determined using sigmoidal dose response equationFloatμM
31Std.Err(IC50)_2Standard Error of IC50 valueFloatμM
32nH_2Hill coefficient determined using sigmoidal dose response equationFloat
33Excluded_Points_third_pointFlags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
34% Activity at 32 uM_third_point (32μM**)% Inhibition at a given concentrationFloat%
35% Activity at 16 uM_third_point (16μM**)% Inhibition at a given concentrationFloat%
36% Activity at 8 uM_third_point (8μM**)% Inhibition at a given concentrationFloat%
37% Activity at 4 uM_third_point (4μM**)% Inhibition at a given concentrationFloat%
38% Activity at 2 uM_third_point (2μM**)% Inhibition at a given concentrationFloat%
39% Activity at 1 uM_third_point (1μM**)% Inhibition at a given concentrationFloat%
40% Activity at 0.5 uM_third_point (0.5μM**)% Inhibition at a given concentrationFloat%
41% Activity at 0.25 uM_third_point (0.25μM**)% Inhibition at a given concentrationFloat%
42% Activity at 0.125 uM_third_point (0.125μM**)% Inhibition at a given concentrationFloat%
43% Activity at 0.0625 uM_third_point (0.0625μM**)% Inhibition at a given concentrationFloat%
44Excluded_Points_fourth_pointFlags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
45% Activity at 32 uM_fourth_point (32μM**)% Inhibition at a given concentrationFloat%
46% Activity at 16 uM_fourth_point (16μM**)% Inhibition at a given concentrationFloat%
47% Activity at 8 uM_fourth_point (8μM**)% Inhibition at a given concentrationFloat%
48% Activity at 4 uM_fourth_point (4μM**)% Inhibition at a given concentrationFloat%
49% Activity at 2 uM_fourth_point (2μM**)% Inhibition at a given concentrationFloat%
50% Activity at 1 uM_fourth_point (1μM**)% Inhibition at a given concentrationFloat%
51% Activity at 0.5 uM_fourth_point (0.5μM**)% Inhibition at a given concentrationFloat%
52% Activity at 0.25 uM_fourth_point (0.25μM**)% Inhibition at a given concentrationFloat%
53% Activity at 0.125 uM_fourth_point (0.125μM**)% Inhibition at a given concentrationFloat%
54% Activity at 0.0625 uM_fourth_point (0.0625μM**)% Inhibition at a given concentrationFloat%
55IC50_Qualifier_3This qualifier is to be used with the next TID, IC50_3. If qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
56IC50_3IC50 value determined using sigmoidal dose response equationFloatμM
57Std.Err(IC50)_3Standard Error of IC50 valueFloatμM
58nH_3Hill coefficient determined using sigmoidal dose response equationFloat
59Excluded_Points_fifth_pointFlags to indicate which of the fifth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
60% Activity at 32 uM_fifth_point (32μM**)% Inhibition at a given concentrationFloat%
61% Activity at 16 uM_fifth_point (16μM**)% Inhibition at a given concentrationFloat%
62% Activity at 8 uM_fifth_point (8μM**)% Inhibition at a given concentrationFloat%
63% Activity at 4 uM_fifth_point (4μM**)% Inhibition at a given concentrationFloat%
64% Activity at 2 uM_fifth_point (2μM**)% Inhibition at a given concentrationFloat%
65% Activity at 1 uM_fifth_point (1μM**)% Inhibition at a given concentrationFloat%
66% Activity at 0.5 uM_fifth_point (0.5μM**)% Inhibition at a given concentrationFloat%
67% Activity at 0.25 uM_fifth_point (0.25μM**)% Inhibition at a given concentrationFloat%
68% Activity at 0.125 uM_fifth_point (0.125μM**)% Inhibition at a given concentrationFloat%
69% Activity at 0.0625 uM_fifth_point (0.0625μM**)% Inhibition at a given concentrationFloat%
70Excluded_Points_sixth_pointFlags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
71% Activity at 32 uM_sixth_point (32μM**)% Inhibition at a given concentrationFloat%
72% Activity at 16 uM_sixth_point (16μM**)% Inhibition at a given concentrationFloat%
73% Activity at 8 uM_sixth_point (8μM**)% Inhibition at a given concentrationFloat%
74% Activity at 4 uM_sixth_point (4μM**)% Inhibition at a given concentrationFloat%
75% Activity at 2 uM_sixth_point (2μM**)% Inhibition at a given concentrationFloat%
76% Activity at 1 uM_sixth_point (1μM**)% Inhibition at a given concentrationFloat%
77% Activity at 0.5 uM_sixth_point (0.5μM**)% Inhibition at a given concentrationFloat%
78% Activity at 0.25 uM_sixth_point (0.25μM**)% Inhibition at a given concentrationFloat%
79% Activity at 0.125 uM_sixth_point (0.125μM**)% Inhibition at a given concentrationFloat%
80% Activity at 0.0625 uM_sixth_point (0.0625μM**)% Inhibition at a given concentrationFloat%
81IC50_Qualifier_4This qualifier is to be used with the next TID, IC50_4. If qualifier is "=", the IC50 result equals the value in that column. If the qualifier is ">", the IC50 result is greater than that value. If the qualifier is "<", the IC50 result is smaller than that valueString
82IC50_4IC50 value determined using sigmoidal dose response equationFloatμM
83Std.Err(IC50)_4Standard Error of IC50 valueFloatμM
84nH_4Hill coefficient determined using sigmoidal dose response equationFloat
85Excluded_Points_seventh_pointFlags to indicate which of the seventh dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
86% Activity at 32 uM_seventh_point (32μM**)% Inhibition at a given concentrationFloat%
87% Activity at 16 uM_seventh_point (16μM**)% Inhibition at a given concentrationFloat%
88% Activity at 8 uM_seventh_point (8μM**)% Inhibition at a given concentrationFloat%
89% Activity at 4 uM_seventh_point (4μM**)% Inhibition at a given concentrationFloat%
90% Activity at 2 uM_seventh_point (2μM**)% Inhibition at a given concentrationFloat%
91% Activity at 1 uM_seventh_point (1μM**)% Inhibition at a given concentrationFloat%
92% Activity at 0.5 uM_seventh_point (0.5μM**)% Inhibition at a given concentrationFloat%
93% Activity at 0.25 uM_seventh_point (0.25μM**)% Inhibition at a given concentrationFloat%
94% Activity at 0.125 uM_seventh_point (0.125μM**)% Inhibition at a given concentrationFloat%
95% Activity at 0.0625 uM_seventh_point (0.0625μM**)% Inhibition at a given concentrationFloat%
96Excluded_Points_eighth_pointFlags to indicate which of the eighth dose-response points were excluded from analysis. (1) means the titration point was excluded and (0) means the point was not excluded.String
97% Activity at 32 uM_eighth_point (32μM**)% Inhibition at a given concentrationFloat%
98% Activity at 16 uM_eighth_point (16μM**)% Inhibition at a given concentrationFloat%
99% Activity at 8 uM_eighth_point (8μM**)% Inhibition at a given concentrationFloat%
100% Activity at 4 uM_eighth_point (4μM**)% Inhibition at a given concentrationFloat%
101% Activity at 2 uM_eighth_point (2μM**)% Inhibition at a given concentrationFloat%
102% Activity at 1 uM_eighth_point (1μM**)% Inhibition at a given concentrationFloat%
103% Activity at 0.5 uM_eighth_point (0.5μM**)% Inhibition at a given concentrationFloat%
104% Activity at 0.25 uM_eighth_point (0.25μM**)% Inhibition at a given concentrationFloat%
105% Activity at 0.125 uM_eighth_point (0.125μM**)% Inhibition at a given concentrationFloat%
106% Activity at 0.0625 uM_eighth_point (0.0625μM**)% Inhibition at a given concentrationFloat%

* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: 1X01DA026208-01

Data Table (Concise)
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