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BioAssay: AID 464320

Activation of archaea Methanobacterium thermoautotrophicum recombinant carbonic anhydrase by stopped flow CO2 hydration method

The protein encoded by the NCE103 gene of Candida glabrata, a beta-carbonic anhydrase (CA, EC 4.2.1.1) designated as CgCA, was investigated for its activation with amines and amino acids. CgCA was weakly activated by amino acids such as l-/d-His, l-Phe, l-DOPA, and l-Trp and by histamine or dopamine (K(A)s of 21.2-37microM) but more effectively activated by d-Phe, d-DOPA, d-Trp as well as more ..
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 Tested Compounds
 Tested Compounds
All(19)
 
 
Active(12)
 
 
Unspecified(7)
 
 
 Tested Substances
 Tested Substances
All(19)
 
 
Active(12)
 
 
Unspecified(7)
 
 
 Related BioAssays
 Related BioAssays
AID: 464320
Data Source: ChEMBL (615183)
Depositor Category: Literature, Extracted
BioAssay Version:
Deposit Date: 2010-09-27
Modify Date: 2014-05-27

Data Table ( Complete ):           Active    All
BioActive Compounds: 12
Description:
Title: Carbonic anhydrase activators: Activation of the beta-carbonic anhydrase from the pathogenic yeast Candida glabrata with amines and amino acids.

Abstract: The protein encoded by the NCE103 gene of Candida glabrata, a beta-carbonic anhydrase (CA, EC 4.2.1.1) designated as CgCA, was investigated for its activation with amines and amino acids. CgCA was weakly activated by amino acids such as l-/d-His, l-Phe, l-DOPA, and l-Trp and by histamine or dopamine (K(A)s of 21.2-37microM) but more effectively activated by d-Phe, d-DOPA, d-Trp as well as serotonin, pyridyl-alkylamines, aminoethyl-piperazine/morpholine (K(A)s of 10.1-16.7microM). The best activators were l-/d-Tyr, with activation constants of 7.1-9.5microM. This study may bring a better understanding of the catalytic/activation mechanisms of beta-CAs from pathogenic fungi.
(PMID: 20129782)
Comment
Compounds with activity <= 50uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Putative Target:

ChEMBL Target ID: 20009
Target Type: SINGLE PROTEIN
Pref Name: Carbonic anhydrase
Synonyms: Carbonic anhydrase;
Protein Accession: Q50565;
Protein GI: 74515025;
Organism: Methanobacterium thermoautotrophicum
Tax ID: 187420
Target Classification: enzyme
Confidence: Homologous single protein target assigned
Relationship Type: Homologous protein target assigned
Categorized Comment
Assay Type: Binding

Assay Data Source: Scientific Literature

BAO: Assay Format: biochemical format

Result Definitions
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
1Ka activity commentKa activity commentString
2Ka standard flagKa standard flagInteger
3Ka qualifierKa qualifierString
4Ka published valueKa published valueFloatμM
5Ka standard valueKa standard valueFloatμM

Data Table (Concise)
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