Compound Screen Assay, Human AASDHPPT
The apparent binding of compounds to Human AASDHPPT has been measured using differential scanning fluorimetry (DSF) technique. ..more
BioActive Compounds: 4
The apparent binding of compounds to Human AASDHPPT has been measured using differential scanning fluorimetry (DSF) technique.
In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compound and an experiment with the compound present is called a TmShift. Positive shifts indicate that the protein is more stable in the presence of the compound, indicating that the compound is bound. Since it is not possible to quantitatively compare TmShift values from different experiments containing different components, we mark positive shifts of more than 3 degrees celcius as having a PubChem assay score of 100, and all other shifts with a score of 0.
AASDHPPT catalyzes the transfer of the 4'-phosphopantetheine moiety of coenzyme A to different carrier proteins, the most significant being the acyl carrier protein domains of cytosolic and mitochondrial fatty acid synthase enzymes. This modification of acyl-carrier proteins associated with type I and II fatty acids synthases is essential for lipid synthesis pathways.
Data Table (Concise)