Bookmark and Share
BioAssay: AID 355714

Binding affinity to bovine brain calmodulin assessed as decrease in electrophoretic mobility at 0.033 ug/ml for 1.5 hrs by SDS-PAGE in presence of Ca2+ ions

Reinvestigation of the fermentation broth and mycelium of the fungus Phoma herbarum led to the isolation of a new phytotoxic nonenolide, namely, (7R,9R)-7-hydroxy-9-propyl-5-nonen-9-olide, which was designated with the trivial name herbarumin III (3). The known compounds herbarumins I (1) and II (2) were also obtained. The structure of 3 was elucidated by spectroscopic methods and molecular modeling. Compounds 1-3 interacted with bovine-brain calmodulin and inhibited the activation of the calmodulin-dependent enzyme cAMP phosphodiesterase. ..more
_
   
 Tested Compounds
 Tested Compounds
All(3)
 
 
Active(3)
 
 
 Tested Substances
 Tested Substances
All(3)
 
 
Active(3)
 
 
 Related BioAssays
 Related BioAssays
AID: 355714
Data Source: ChEMBL (503743)
Depositor Category: Literature, Extracted
BioAssay Version:
Deposit Date: 2010-05-25
Modify Date: 2013-11-20

Data Table ( Complete ):           Active    All
Target
Sequence: RecName: Full=Calmodulin; Short=CaM
Description ..   
Protein Family: EFh
Comment ..   

Gene:CALM     Related Protein 3D Structures     More BioActivity Data..
BioActive Compounds: 3
Description:
Title: A new phytotoxic nonenolide from Phoma herbarum.

Abstract: Reinvestigation of the fermentation broth and mycelium of the fungus Phoma herbarum led to the isolation of a new phytotoxic nonenolide, namely, (7R,9R)-7-hydroxy-9-propyl-5-nonen-9-olide, which was designated with the trivial name herbarumin III (3). The known compounds herbarumins I (1) and II (2) were also obtained. The structure of 3 was elucidated by spectroscopic methods and molecular modeling. Compounds 1-3 interacted with bovine-brain calmodulin and inhibited the activation of the calmodulin-dependent enzyme cAMP phosphodiesterase.
(PMID: 12713403)
Categorized Comment
ChEMBL Assay Type: Binding

ChEMBL Assay Data Source: Scientific Literature

ChEMBL Target ID: 101443

ChEMBL Target Type: Target is a single protein chain

Result Definitions
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
1Activity activity commentActivity activity commentString
2Activity standard flagActivity standard flagInteger
3Activity qualifierActivity qualifierString
4Activity published valueActivity published valueFloat
5Activity standard valueActivity standard valueFloat

Data Table (Concise)
Classification
PageFrom: