The Renal Outer Medullary Potassium channel (ROMK, Kir1.1) is expressed in the renal tubule where it critically regulates fluid and electrolyte homeostasis (Hebert, 2005). An emerging body of evidence suggests that ROMK could be a target for a novel class loop diuretic that lowers blood pressure while preserving plasma potassium levels (Ji, 2008). Furthermore, homozygous loss-of-function more ..
Related BioAssays
Related BioAssays
Target
BioActive Compounds: 114
Depositor Specified Assays
| AID | Name | Type | Comment |
|---|---|---|---|
| 1917 | High throughput discovery of novel modulators of ROMK K+ channel activity: Retest of Primary Hits | other | |
| 1918 | High throughput discovery of novel modulators of ROMK K+ channel activity: Primary Screen | screening | |
| 1922 | High throughput discovery of novel modulators of ROMK K+ channel activity: Selectivity by Patch Clamp | other | |
| 2436 | High-throughput Discovery of Novel Modulators of ROMK K+ Channel Activity | summary |
Description:
Assay Provider: Jerod Denton
Assay Provider Affiliation: Vanderbilt University
Grant Title: High throughput discovery of novel modulators of ROMK K+ channel activity
Grant Number: R21 NS057041-01
The Renal Outer Medullary Potassium channel (ROMK, Kir1.1) is expressed in the renal tubule where it critically regulates fluid and electrolyte homeostasis (Hebert, 2005). An emerging body of evidence suggests that ROMK could be a target for a novel class loop diuretic that lowers blood pressure while preserving plasma potassium levels (Ji, 2008). Furthermore, homozygous loss-of-function mutations in the gene encoding ROMK (KCNJ1) cause antenatal Bartter syndrome, a severe salt and water wasting disease in infants (Simon, 1996). ROMK is thus an important pharmacological target for the management of disease. Its actual therapeutic value and drugability, however, are unknown due to the lack of small-molecule probes targeting the channel. The discovery of ROMK modulators will provide important new tools for studying the structure, function and therapeutic potential of ROMK and other inward rectifying potassium channels.
Assay Provider Affiliation: Vanderbilt University
Grant Title: High throughput discovery of novel modulators of ROMK K+ channel activity
Grant Number: R21 NS057041-01
The Renal Outer Medullary Potassium channel (ROMK, Kir1.1) is expressed in the renal tubule where it critically regulates fluid and electrolyte homeostasis (Hebert, 2005). An emerging body of evidence suggests that ROMK could be a target for a novel class loop diuretic that lowers blood pressure while preserving plasma potassium levels (Ji, 2008). Furthermore, homozygous loss-of-function mutations in the gene encoding ROMK (KCNJ1) cause antenatal Bartter syndrome, a severe salt and water wasting disease in infants (Simon, 1996). ROMK is thus an important pharmacological target for the management of disease. Its actual therapeutic value and drugability, however, are unknown due to the lack of small-molecule probes targeting the channel. The discovery of ROMK modulators will provide important new tools for studying the structure, function and therapeutic potential of ROMK and other inward rectifying potassium channels.
Protocol
The purpose of this assay to test synthesized compounds in thallium flux for their ability to dose-dependently inhibit ROMK function.
Experimental methods were as previously described (Lewis, 2009). Briefly, cells were plated in black-walled, clear-bottom plates and treated with Tet overnight to induce the expression of ROMK in serum-free media. The cells were loaded with FluoZin2 dye, incubated for 20 min at RT and washed with assay buffer (0.44 mM NaH2PO4, 4.17 mM NaHCO3, 137.93 mM NaCl, 0.338 mM Na2HPO4, 20 mM HEPES, 0.25 mM K2SO4, adjusted to 343 mOsm with sucrose). The plate was imaged on the Hamamatsu FDSS 6000 system to obtain F0, followed by compound addition. Compounds were serially diluted 3-fold to generate 11-point concentration curves. After 20 min at RT, thallium stimulus buffer (125 mM sodium gluconate, 12 mM thallium sulfate, 1 mM magnesium sulfate, 1.8 mM calcium gluconate, 5 mM glucose, 10 mM HEPES, pH 7.3, adjusted to 343 mOsm with sucrose) was added while simultaneously imaging for a total of 2 min acquisition time.
Data Handling:
The kinetic fluorescence values (F) from each well were divided by the initial frame of the read (F0) to give the static ratio (F/F0) which corrects for variability in cell number and dye loading. The slope of the static ratio from 7 to 12 seconds was calculated for each compound concentration to give 'Value'. An automated data analysis pipeline generated with Pipeline Pilot (Accelrys, San Diego, CA) and R statistics package (www.r-project.org) was used. TPNQ and 5mM potassium buffer control wells were included on every plate and used to calculate the Z' (Zhang et al., 1999). Pubchem 'Outcome' and 'Activity' were assigned as follows: 1) samples with 2 or more replicates with 'Fit' were 'Active' and '100'; 2) samples with 2 or more replicates with 'Trend' were 'Active' and '50'; 3)samples with 1 'Fit' and 1 'Trend' were 'Active' and '50'; and 4)all other samples were 'Inactive' and '0'.
Experimental methods were as previously described (Lewis, 2009). Briefly, cells were plated in black-walled, clear-bottom plates and treated with Tet overnight to induce the expression of ROMK in serum-free media. The cells were loaded with FluoZin2 dye, incubated for 20 min at RT and washed with assay buffer (0.44 mM NaH2PO4, 4.17 mM NaHCO3, 137.93 mM NaCl, 0.338 mM Na2HPO4, 20 mM HEPES, 0.25 mM K2SO4, adjusted to 343 mOsm with sucrose). The plate was imaged on the Hamamatsu FDSS 6000 system to obtain F0, followed by compound addition. Compounds were serially diluted 3-fold to generate 11-point concentration curves. After 20 min at RT, thallium stimulus buffer (125 mM sodium gluconate, 12 mM thallium sulfate, 1 mM magnesium sulfate, 1.8 mM calcium gluconate, 5 mM glucose, 10 mM HEPES, pH 7.3, adjusted to 343 mOsm with sucrose) was added while simultaneously imaging for a total of 2 min acquisition time.
Data Handling:
The kinetic fluorescence values (F) from each well were divided by the initial frame of the read (F0) to give the static ratio (F/F0) which corrects for variability in cell number and dye loading. The slope of the static ratio from 7 to 12 seconds was calculated for each compound concentration to give 'Value'. An automated data analysis pipeline generated with Pipeline Pilot (Accelrys, San Diego, CA) and R statistics package (www.r-project.org) was used. TPNQ and 5mM potassium buffer control wells were included on every plate and used to calculate the Z' (Zhang et al., 1999). Pubchem 'Outcome' and 'Activity' were assigned as follows: 1) samples with 2 or more replicates with 'Fit' were 'Active' and '100'; 2) samples with 2 or more replicates with 'Trend' were 'Active' and '50'; 3)samples with 1 'Fit' and 1 'Trend' were 'Active' and '50'; and 4)all other samples were 'Inactive' and '0'.
Result Definitions
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| TID | Name | Description | Histogram | Type | Unit | |
|---|---|---|---|---|---|---|
| Outcome | The BioAssay activity outcome | Outcome | ||||
| Score | The BioAssay activity ranking score |  | Integer | |||
| 1 | VUID | Vanderbilt internal identifier | String | |||
| 2 | FitCategory for replicate 1 | Qualitative assessment of the quality of the curve. Options are "fit," "trend," and "no fit" for replicate 1 | String | |||
| 3 | FitCategory for replicate 2 | Qualitative assessment of the quality of the curve. Options are "fit," "trend," and "no fit" for replicate 2 | String | |||
| 4 | FitCategory for replicate 3 | Qualitative assessment of the quality of the curve. Options are "fit," "trend," and "no fit" for replicate 3 | String | |||
| 5 | top for replicate 1 | If the fit converged, then this is the calculated value for the top parameter for replicate 1 | | Float | ||
| 6 | top for replicate 2 | If the fit converged, then this is the calculated value for the top parameter for replicate 2 | | Float | ||
| 7 | top for replicate 3 | If the fit converged, then this is the calculated value for the top parameter for replicate 3 | | Float | ||
| 8 | bottom for replicate 1 | If the fit converged, then this is the calculated value for the bottom parameter for replicate 1 | | Float | ||
| 9 | bottom for replicate 2 | If the fit converged, then this is the calculated value for the bottom parameter for replicate 2 | | Float | ||
| 10 | bottom for replicate 3 | If the fit converged, then this is the calculated value for the bottom parameter for replicate 3 | | Float | ||
| 11 | slope for replicate 1 | If the fit converged, then this is the calculated value for the slope parameter for replicate 1 | | Float | ||
| 12 | slope for replicate 2 | If the fit converged, then this is the calculated value for the slope parameter for replicate 2 | | Float | ||
| 13 | slope for replicate 3 | If the fit converged, then this is the calculated value for the slope parameter for replicate 3 | | Float | ||
| 14 | logEC50 for replicate 1 | If the fit converged, then this is the calculated value for theLogEC50 parameter for replicate 1 | | Float | ||
| 15 | logEC50 for replicate 2 | If the fit converged, then this is the calculated value for theLogEC50 parameter for replicate 2 | | Float | ||
| 16 | logEC50 for replicate 3 | If the fit converged, then this is the calculated value for theLogEC50 parameter for replicate 3 | | Float | ||
| 17 | EC50 for replicate 1 | EC50 value in micromolar for replicate 1 | | Float | μM | |
| 18 | EC50 for replicate 2 | EC50 value in micromolar for replicate 2 | | Float | μM | |
| 19 | EC50 for replicate 3* | EC50 value in micromolar for replicate 3 | | Float | μM | |
| 20 | top_stderr for replicate 1 | Standard error for the calculated top value for replicate 1 | | Float | ||
| 21 | top_stderr for replicate 2 | Standard error for the calculated top value for replicate 2 | | Float | ||
| 22 | top_stderr for replicate 3 | Standard error for the calculated top value for replicate 3 | | Float | ||
| 23 | bottom_stderr for replicate 1 | Standard error for the calculated bottom value for replicate 1 | | Float | ||
| 24 | bottom_stderr for replicate 2 | Standard error for the calculated bottom value for replicate 2 | | Float | ||
| 25 | bottom_stderr for replicate 3 | Standard error for the calculated bottom value for replicate 3 | | Float | ||
| 26 | slope_stderr for replicate 1 | Standard error for the calculated slope value for replicate 1 | | Float | ||
| 27 | slope_stderr for replicate 2 | Standard error for the calculated slope value for replicate 2 | | Float | ||
| 28 | slope_stderr for replicate 3 | Standard error for the calculated slope value for replicate 3 | | Float | ||
| 29 | logEC50_stderr for replicate 1 | Standard error for the calculated LogEC50 value for replicate 1 | | Float | ||
| 30 | logEC50_stderr for replicate 2 | Standard error for the calculated LogEC50 value for replicate 2 | | Float | ||
| 31 | logEC50_stderr for replicate 3 | Standard error for the calculated LogEC50 value for replicate 3 | | Float | ||
| 32 | EC50_stderr for replicate 1 | Standard error for the calculated EC50 value for replicate 1 | | Float | ||
| 33 | EC50_stderr for replicate 2 | Standard error for the calculated EC50 value for replicate 2 | | Float | ||
| 34 | EC50_stderr for replicate 3 | Standard error for the calculated EC50 value for replicate 3 | | Float | ||
| 35 | top_pr for replicate 1 | p value for the calculated tope value for replicate 1 | | Float | ||
| 36 | top_pr for replicate 2 | p value for the calculated tope value for replicate 2 | | Float | ||
| 37 | top_pr for replicate 3 | p value for the calculated tope value for replicate 3 | | Float | ||
| 38 | bottom_pr for replicate 1 | p value for the calculated bottom value for replicate 1 | | Float | ||
| 39 | bottom_pr for replicate 2 | p value for the calculated bottom value for replicate 2 | | Float | ||
| 40 | bottom_pr for replicate 3 | p value for the calculated bottom value for replicate 3 | | Float | ||
| 41 | slope_pr for replicate 1 | p value for the calculated slope for replicate 1 | | Float | ||
| 42 | slope_pr for replicate 2 | p value for the calculated slope for replicate 2 | | Float | ||
| 43 | slope_pr for replicate 3 | p value for the calculated slope for replicate 3 | | Float | ||
| 44 | logEC50_pr for replicate 1 | p value for the calculated logEC50 for replicate 1 | | Float | ||
| 45 | logEC50_pr for replicate 2 | p value for the calculated logEC50 for replicate 2 | | Float | ||
| 46 | logEC50_pr for replicate 3 | p value for the calculated logEC50 for replicate 3 | | Float | ||
| 47 | 5mM_mean for replicate 1 | Mean of 5mM potassium positive control on a per-plate-basis for replicate 1 | | Float | ||
| 48 | 5mM_mean for replicate 2 | Mean of 5mM potassium positive control on a per-plate-basis for replicate 2 | | Float | ||
| 49 | 5mM_mean for replicate 3 | Mean of 5mM potassium positive control on a per-plate-basis for replicate 3 | | Float | ||
| 50 | 5mM_stddev for replicate 1 | Standard deviation of mean for 5mM potassium positive control on a per-plate-basis for replicate 1 | | Float | ||
| 51 | 5mM_stddev for replicate 2 | Standard deviation of mean for 5mM potassium positive control on a per-plate-basis for replicate 2 | | Float | ||
| 52 | 5mM_stddev for replicate 3 | Standard deviation of mean for 5mM potassium positive control on a per-plate-basis for replicate 3 | | Float | ||
| 53 | TPNQ_mean for replicate 1 | Mean of TPNQ negative control on a per-plate-basis for replicate 1 | | Float | ||
| 54 | TPNQ_mean for replicate 2 | Mean of TPNQ negative control on a per-plate-basis for replicate 2 | | Float | ||
| 55 | TPNQ_mean for replicate 3 | Mean of TPNQ negative control on a per-plate-basis for replicate 3 | | Float | ||
| 56 | TPNQ_stddev for replicate 1 | Standard deviation of mean for TPNQ negative control on a per-plate-basis for replicate 1 | | Float | ||
| 57 | TPNQ_stddev for replicate 2 | Standard deviation of mean for TPNQ negative control on a per-plate-basis for replicate 2 | | Float | ||
| 58 | TPNQ_stddev for replicate 3 | Standard deviation of mean for TPNQ negative control on a per-plate-basis for replicate 3 | | Float | ||
| 59 | zprime_5mM_TPNQ for replicate 1 | The calculated z' value between the TPNQ and 5mM Potassium controls on the plate for replicate 1 | | Float | ||
| 60 | zprime_5mM_TPNQ for replicate 2 | The calculated z' value between the TPNQ and 5mM Potassium controls on the plate for replicate 2 | | Float | ||
| 61 | zprime_5mM_TPNQ for replicate 3 | The calculated z' value between the TPNQ and 5mM Potassium controls on the plate for replicate 3 | | Float | ||
| 62 | Value at 2.54e-003 micromolar for replicate 1 (0.00254μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 63 | Value at 2.54e-003 micromolar for replicate 2 (0.00254μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 64 | Value at 2.54e-003 micromolar for replicate 3 (0.00254μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 65 | Value at 7.62e-003 micromolar for replicate 1 (0.00762μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 66 | Value at 7.62e-003 micromolar for replicate 2 (0.00762μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 67 | Value at 7.62e-003 micromolar for replicate 3 (0.00762μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 68 | Value at 2.29e-002 micromolar for replicate 1 (0.0229μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 69 | Value at 2.29e-002 micromolar for replicate 2 (0.0229μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 70 | Value at 2.29e-002 micromolar for replicate 3 (0.0229μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 71 | Value at 6.86e-002 micromolar for replicate 1 (0.0686μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 72 | Value at 6.86e-002 micromolar for replicate 2 (0.0686μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 73 | Value at 6.86e-002 micromolar for replicate 3 (0.0686μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 74 | Value at 0.206 micromolar for replicate 1 (0.206μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 75 | Value at 0.206 micromolar for replicate 2 (0.206μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 76 | Value at 0.206 micromolar for replicate 3 (0.206μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 77 | Value at 0.617 micromolar for replicate 1 (0.617μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 78 | Value at 0.617 micromolar for replicate 2 (0.617μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 79 | Value at 0.617 micromolar for replicate 3 (0.617μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 80 | Value at 1.85 micromolar for replicate 1 (1.85μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 81 | Value at 1.85 micromolar for replicate 2 (1.85μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 82 | Value at 1.85 micromolar for replicate 3 (1.85μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 83 | Value at 5.56 micromolar for replicate 1 (5.56μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 84 | Value at 5.56 micromolar for replicate 2 (5.56μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 85 | Value at 5.56 micromolar for replicate 3 (5.56μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 86 | Value at 16.7 micromolar for replicate 1 (16.7μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 87 | Value at 16.7 micromolar for replicate 2 (16.7μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 88 | Value at 16.7 micromolar for replicate 3 (16.7μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 89 | Value at 50 micromolar for replicate 1 (50μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 90 | Value at 50 micromolar for replicate 2 (50μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 91 | Value at 50 micromolar for replicate 3 (50μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 92 | Value at 150 micromolar for replicate 1 (150μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 93 | Value at 150 micromolar for replicate 2 (150μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 94 | Value at 150 micromolar for replicate 3 (150μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 95 | Value at 30 micromolar for replicate 1 (30μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 96 | Value at 30 micromolar for replicate 2 (30μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 97 | Value at 30 micromolar for replicate 3 (30μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 98 | Value at 10 micromolar for replicate 1 (10μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 99 | Value at 10 micromolar for replicate 2 (10μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 100 | Value at 10 micromolar for replicate 3 (10μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 101 | Value at 3.33 micromolar for replicate 1 (3.33μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 102 | Value at 3.33 micromolar for replicate 2 (3.33μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 103 | Value at 3.33 micromolar for replicate 3 (3.33μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 104 | Value at 1.11 micromolar for replicate 1 (1.11μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 105 | Value at 1.11 micromolar for replicate 2 (1.11μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 106 | Value at 1.11 micromolar for replicate 3 (1.11μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 107 | Value at 0.37 micromolar for replicate 1 (0.37μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 108 | Value at 0.37 micromolar for replicate 2 (0.37μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 109 | Value at 0.37 micromolar for replicate 3 (0.37μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 110 | Value at 0.123 micromolar for replicate 1 (0.123μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 111 | Value at 0.123 micromolar for replicate 2 (0.123μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 112 | Value at 0.123 micromolar for replicate 3 (0.123μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 113 | Value at 4.12e-002 micromolar for replicate 1 (0.0412μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 114 | Value at 4.12e-002 micromolar for replicate 2 (0.0412μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 115 | Value at 4.12e-002 micromolar for replicate 3 (0.0412μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 116 | Value at 1.37e-002 micromolar for replicate 1 (0.0137μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 117 | Value at 1.37e-002 micromolar for replicate 2 (0.0137μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 118 | Value at 1.37e-002 micromolar for replicate 3 (0.0137μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 119 | Value at 4.57e-003 micromolar for replicate 1 (0.00457μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 120 | Value at 4.57e-003 micromolar for replicate 2 (0.00457μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 121 | Value at 4.57e-003 micromolar for replicate 3 (0.00457μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 122 | Value at 1.52e-003 micromolar for replicate 1 (0.00152μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 123 | Value at 1.52e-003 micromolar for replicate 2 (0.00152μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 124 | Value at 1.52e-003 micromolar for replicate 3 (0.00152μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float | ||
| 125 | Value at 5.08e-004 micromolar for replicate 1 (0.000508μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 1 | | Float | ||
| 126 | Value at 5.08e-004 micromolar for replicate 2 (0.000508μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 2 | | Float | ||
| 127 | Value at 5.08e-004 micromolar for replicate 3 (0.000508μM**) | The raw fluorescence intensities were divided by the initial fluorescence, and the slope after thallium addition was calculated for replicate 3 | | Float |
* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: R21 NS057041-01
Data Table (Concise)
Classification
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