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BioAssay: AID 2336

Summary Assay for Inhibitors of Influenza NS1 Protein Function

Influenza is a world-wide public health problem and emerging forms of the virus have the potential to cause a pandemic of equal or greater magnitude to the outbreaks recorded in 1918, 1957 or 1968. Vaccine development is proceeding and there also exist two classes of anti-influenza compounds. However these therapeutic modalities are neither fully effective nor widely enough available to fulfill more ..
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AID: 2336
Data Source: NCGC (NS100051)
BioAssay Type: Summary, Candidate Probes/Leads with Supporting Evidence
Depositor Category: NIH Molecular Libraries Probe Production Network
Deposit Date: 2010-02-04
Target
Related Experiments
AIDNameTypeComment
2326qHTS Assay for Inhibitors of Influenza NS1 Protein FunctionConfirmatorydepositor-specified cross reference
492980Assay for Inhibitors of Influenza NS1 Protein Function: Hit ValidationConfirmatorydepositor-specified cross reference
504647Assay for Inhibitors of Influenza NS1 Protein Function: Influenza virus replication assay in MDCK cells infected with virus A/PR/8/34Otherdepositor-specified cross reference
602450qHTS Assay for Inhibitors of Influenza NS1 Protein Function: Viral Replication TCID50 SAR for ProbeOtherdepositor-specified cross reference
602452qHTS Assay for Inhibitors of Influenza NS1 Protein Function: SAR Using NS1 and pYES strainsOtherdepositor-specified cross reference
602454qHTS Assay for Inhibitors of Influenza NS1 Protein Function: Viral Replication TCID50Otherdepositor-specified cross reference
602456qHTS Assay for Inhibitors of Influenza NS1 Protein Function: RT-PCROtherdepositor-specified cross reference
623997qHTS Assay for Inhibitors of Influenza NS1 Protein Function: Mammalian Cell ViabilityOtherdepositor-specified cross reference
Description:
NIH Chemical Genomics Center [NCGC]
NIH Molecular Libraries Probe Centers Network [MLPCN]

MLPCN Grant: MH084878-01A1
Assay Submitter (PI): Daniel Engel

Influenza is a world-wide public health problem and emerging forms of the virus have the potential to cause a pandemic of equal or greater magnitude to the outbreaks recorded in 1918, 1957 or 1968. Vaccine development is proceeding and there also exist two classes of anti-influenza compounds. However these therapeutic modalities are neither fully effective nor widely enough available to fulfill global needs. In addition their potential usefulness against newly emergent strains is not known. Efforts are needed to develop novel agents against influenza virus, including broad-spectrum agents. Identification of small molecules that inhibit NS1 function either directly or by interfering with specific cellular pathways may be a key to increasing our defense against the virus.

We have miniaturized and optimized a cell based assay in which NS1 from influenza A is expressed in the budding yeast S. cerevisiae. NS1 is a multi-functional protein that counters the host innate immune response and facilitates viral versus cellular gene expression. Expression of NS1 causes a pronounced slow growth phenotype in yeast due to its intrinsic molecular activities. Small molecules that suppress the slow growth phenotype can be identified by a straightforward growth recovery assay using optical density (OD) as the measurement. The same yeast strain not expressing NS1 was used as positive control in the yeast growth recovery assay.
Protocol
Please refer to AID 2326 for detailed assay protocols.
Comment
This summary is written for the purposes of summarizing the probe activities from the project. MLSCN probes are given a score of 100. Molecules in the literature are given a score of 80. Other, less active molecules in the same chemical series as the probe molecules are given a score of 50. Molecules pending validation are given a score of 10. Inactive analogues from these series are given a score of 0. No compounds from this project have yet been validated.
Categorized Comment - additional comments and annotations
From MLP Probe Report:
Probe count: 1
MLP Probe ML# for probe 1: ML303
PubChem Substance ID (SID) for probe 1: 124954998
PubChem Compound ID (CID) for probe 1: 53316412
Probe type for probe 1: Antagonist
IC50/EC50 (nM) for probe 1: 155
Target for probe 1: NS1 (gi: 194352380)
Disease relevance for probe 1: Influenza
Anti-target for probe 1: Cytotoxicity (MDCK)
Fold selectivity for probe 1: >100
NCBI Book chapter link for probe 1: http://www.ncbi.nlm.nih.gov/books/NBK169451/ (ID: 3060698)
Grant number for probe 1: MH085680-01
NCBI Book chapter title for probe 1: Discovery of Small Molecule Influenza Virus NS1 Antagonist
Additional Information
Grant Number: MH084878-01A1

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