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BioAssay: AID 2028

Summary assay for the identification of translation initiation inhibitors (eIF4H)

Translation is an essential cellular process whose deregulation is associated with alterations in cell growth, cell cycle progression, and cell death responses. The initiation phase of translation is a key target for regulation when cells are exposed to various environmental cues (e.g. insulin, amino acid starvation, mitogenic stimulation, hypoxia, etc). As well, translation initiation control is more ..
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AID: 2028
Data Source: Burnham Center for Chemical Genomics (BCCG-A243-eIF4H-Summary-Assay)
BioAssay Type: Summary, Candidate Probes/Leads with Supporting Evidence
Depositor Category: NIH Molecular Libraries Probe Production Network
BioAssay Version:
Deposit Date: 2009-10-26
Modify Date: 2011-01-13
Target
Related Experiments
AIDNameTypeComment
2012uHTS fluorescence polarization assay for the identification of translation initiation inhibitors (eIF4H)Confirmatorydepositor-specified cross reference: uHTS fluorescence polarization assay for the identification of translation initiation inhibitors (eI
435011SAR analysis for the identification of translation initiation inhibitors (eIF4H)Confirmatorydepositor-specified cross reference: SAR Analysis
449744SAR analysis for the identification of translation initiation inhibitors (eIF4H) via a crosslinking assayOtherdepositor-specified cross reference: SAR Analysis
Description:
Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG)
Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA)
Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN)
Grant Number: 1R03MH084835-01
Assay Provider: Jerry Pelletier, Ph.D, McGill University, Montreal, Canada

Translation is an essential cellular process whose deregulation is associated with alterations in cell growth, cell cycle progression, and cell death responses. The initiation phase of translation is a key target for regulation when cells are exposed to various environmental cues (e.g. insulin, amino acid starvation, mitogenic stimulation, hypoxia, etc). As well, translation initiation control is usurped upon viral infection and is deregulated in many human cancers. Over-expression of certain translation factors can lead to malignant transformation and many of the components of the translational apparatus are over-expressed in human cancers. Several tumor suppressor genes directly influence the translation process and recently, chemoresistance in vivo has been linked to deregulated translation initiation. In a transformed setting, where translation can be inhibited by a small molecule modulator (e.g. rapamycin), decreased translation rates are associated with reversal of chemoresistance, possibly by inhibition of pro-survival pathways or resetting of pro-apoptotic program. These results validate translation initiation as a potential chemotherapeutic target.

This assay summarizes the screening activities for this project. No compounds were identified as potential probes and the project has been closed.
Protocol
Please see pertinent AIDs: 2012, 435011,449744
Comment
No potential probe molecules were identified and work has stopped on this project
Additional Information
Grant Number: 1R03MH084835-01

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