Bookmark and Share
BioAssay: AID 198301

Cleavage rate against hairpin ribozyme at 2 mM, in presence of 0.5 uM magnesium ions

Combinations of the polyamine spermine and magnesium ions synergize to dramatically enhance cleavage of the hairpin ribozyme. Certain synthetic basic tripeptides stimulate hairpin cleavage significantly at limiting magnesium ion concentration, notably the tripeptide of L-diaminobutyric acid (Dab). Of a range of novel synthetic spermine-amino acid conjugates, L-Dab-spermine (but not D-Dab nor other amino acid conjugates) was more effective than spermine itself. ..more
_
   
 Tested Compounds
 Tested Compounds
All(18)
 
 
Unspecified(18)
 
 
 Tested Substances
 Tested Substances
All(18)
 
 
Unspecified(18)
 
 
 Related BioAssays
 Related BioAssays
AID: 198301
Data Source: ChEMBL (195575)
Depositor Category: Literature, Extracted
BioAssay Version:
Deposit Date: 2010-05-20
Modify Date: 2014-05-18

Data Table ( Complete ):           View All Data
Tested Compounds:
Description:
Title: Novel spermine-amino acid conjugates and basic tripeptides enhance cleavage of the hairpin ribozyme at low magnesium ion concentration.

Abstract: Combinations of the polyamine spermine and magnesium ions synergize to dramatically enhance cleavage of the hairpin ribozyme. Certain synthetic basic tripeptides stimulate hairpin cleavage significantly at limiting magnesium ion concentration, notably the tripeptide of L-diaminobutyric acid (Dab). Of a range of novel synthetic spermine-amino acid conjugates, L-Dab-spermine (but not D-Dab nor other amino acid conjugates) was more effective than spermine itself.
(PMID: 11714598)
Comment
Putative Target:
ChEMBL Target ID: 100259
Target Type: NUCLEIC-ACID
Pref Name: Hairpin ribozyme RNA
Confidence: Target assigned is molecular non-protein target
Relationship Type: Molecular target other than protein assigned
Categorized Comment - additional comments and annotations
From ChEMBL:
Assay Type: Binding
Assay Data Source: Scientific Literature
Result Definitions
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
1K obs activity commentK obs activity commentString
2K obs standard flagK obs standard flagInteger
3K obs qualifierK obs qualifierString
4K obs published valueK obs published valueFloat
5K obs standard valueK obs standard valueFloat

Data Table (Concise)
Data Table ( Complete ):     View All Data
PageFrom: