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BioAssay: AID 1957

SAR VHR1 Fluorescent Assay for In Vitro dose response studies

Protein tyrosine phosphatases (PTPs) play vital roles in numerous cellular processes and are implicated in a growing number of human diseases, ranging from cancer to cardiovascular, immunological, infectious, neurological, and metabolic diseases. The Vaccinia H1-related (VHR) PTP is a dual-specific Erk and Jnk phosphatase, the loss of which causes specific cell cycle arrest in HeLa carcinoma more ..
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 Tested Compounds
 Tested Compounds
All(75)
 
 
Active(36)
 
 
Inactive(40)
 
 
 Tested Substances
 Tested Substances
All(76)
 
 
Active(36)
 
 
Inactive(40)
 
 
AID: 1957
Data Source: Burnham Center for Chemical Genomics (BCCG-A220-VHR1-DryPowder-Fluorescent-Assay)
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Depositor Category: NIH Molecular Libraries Probe Production Network
BioAssay Version:
Deposit Date: 2009-09-28
Modify Date: 2010-12-30

Data Table ( Complete ):           Active    All
Target
BioActive Compounds: 36
Depositor Specified Assays
AIDNameTypeProbeComment
1654uHTS absorbance assay for the identification of compounds that inhibit VHR1.confirmatory
1878Fluorescent assay for identification of compounds that inhibit VHR1confirmatory
1661Summary of the absorbance assay for the identification of compounds that inhibit VHR1.summary1
1992HTS Colorimetric assay for the identification of compounds that inhibit VHR1screening
2004SAR Colorimetric assay for the identification of compounds that inhibit VHR1confirmatory
2070MOA VHR1 Fluorescent secondary assay for identification of redox-state modulating compoundsconfirmatory
2085Summary assay for inhibitors of HePTPsummary2
449733SAR analysis of compounds that inhibit VHR1, Fluorescent Assay - Set 2confirmatory
Description:
Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG)
Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA)
Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN)
Grant Number: 1 R03 MH084230-01A1
Assay Provider: Dr. Lutz Tautz, Sanford-Burnham Medical Research Institute, San Diego CA

Protein tyrosine phosphatases (PTPs) play vital roles in numerous cellular processes and are implicated in a growing number of human diseases, ranging from cancer to cardiovascular, immunological, infectious, neurological, and metabolic diseases. The Vaccinia H1-related (VHR) PTP is a dual-specific Erk and Jnk phosphatase, the loss of which causes specific cell cycle arrest in HeLa carcinoma cells, suggesting that VHR inhibition may be a useful approach to halt the growth of cancer cells without detrimental effects on normal cells. Recent studies by collaborators and us suggest that VHR is upregulated in several cervix cancer cell lines, in squamous intraepithelial lesions, and squamous cell carcinomas of the uterine cervix.

This biochemical assay employs a fluorescent readout based on the enzyme's ability to liberate methyl-fluorescein from 3-O-methylflyorescein phosphate (OMFP).

References:

Tautz L. and Mustelin T. 2007. Methods 42:250-60.
Protocol
Assay materials:
1) VHR1 was obtained from the assay provider's laboratory.
2) Assay Buffer: 20mM Bis-Tris pH 6.0, 1mM DTT, 0.005% Tween-20
3) 3-O-Methylfluorescein phosphate (OMFP) cyclohexylammonium salt (Sigma-Aldrich, M2629)
4) VHR1 working solution: 3 nM VHR1 solution in the assay buffer
4) OMFP working solution: 25 uM OMFP in the assay buffer


Procedure for dose-response confirmation assay

1) Dispense 10 ul of OMFP working solution into all columns of black small-volume Greiner plates (784076)
2) Dispense 10 uL of assay buffer in columns 1-2.
3) Add serially diluted compounds to columns 3-22 and DMSO to columns 1-2 and 23-24. Final concentration of DMSO was 1.575%
4) Dispense 10 of VHR1 working solution to wells in columns 3 through 24.
5) Incubate lidded plate for 1 hour at room temp.
6) Read plate on M5 plate reader in fluorescence mode (ex: 480nm; 3m: 520nm; dichroic mirror is 515 nm)
Comment
The compounds were tested in 1 or more concentration ranges.

Range1 0-100 uM
Range2 0-50
Range3 0-10 uM
Range4 0-1 uM

For all samples in each range that resolved to an IC50 the results were averaged and reported as IC50_Range. The IC50 results for all of the IC50s were averaged to produce IC50_Mean.

Compounds with an IC50_Mean <= 100 uM are considered to be active in this assay.

To simplify the distinction between the inactives of the primary screen and of the confirmatory screening stage, the Tiered Activity Scoring System was developed and implemented.

Activity Scoring
Activity scoring rules were devised to take into consideration compound efficacy, its potential interference with the assay and the screening stage that the data was obtained. Details of the Scoring System will be published elsewhere. Briefly, the outline of the scoring system is as follows:

1) First tier (0-40 range) is reserved for primary screening data data and is not applicable to this assay.

2) Second tier (41-80 range) is reserved for dose-response confirmation data and is not applicable to this assay.

3) Third tier (81-100 range) is reserved for resynthesized true positives and their analogues

a. Inactive compounds of the confirmatory stage are assigned a score value equal 81.
b. The score is linearly correlated with a compound's inhibitory potency and, in addition, provides a measure of the likelihood that the compound is not an artifact based on the available information.
c. The Hill coefficient is taken as a measure of compound behavior in the assay via an additional scaling factor QC:
QC = 2.6*[exp(-0.5*nH^2) - exp(-1.5*nH^2)]
This empirical factor prorates the likelihood of target-specific compound effect vs. its non-specific behavior in the assay. This factor is based on expectation that a compound with a single mode of action that achieved equilibrium in the TNAP inhibition assay demonstrates the Hill coefficient value of 1. Compounds deviating from that behavior are penalized proportionally to the degree of their deviation.
d. Summary equation that takes into account the items discussed above is
Score = 82 + 3*(pIC50 - 3)*QC,
where pIC50 is a negative log(10) of the IC50 value expressed in mole/L concentration units. This equation results in the Score values above 50 for compounds that demonstrate high potency and predictable behavior. Compounds that are inactive in the assay or whose concentration-dependent behavior are likely to be an artifact of that assay will generally have lower Score values.
Result Definitions
Show more
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1IC50_Mean_QualifierThis qualifier is to be used with the next TID, IC50_Mean. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
2IC50_Mean*IC50 value determined using sigmoidal dose response equationFloatμM
3IC50_Qualifier_1_Range1This qualifier is to be used with the next TID, IC50_1_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
4IC50_1_Range1IC50 value determined using sigmoidal dose response equationFloatμM
5Std.Err(IC50)_1_Range1Standard Error of IC50 valueFloatμM
6nH_1_Range1Hill coefficient determined using sigmoidal dose response equationFloat
7Excluded_Points_first_point_Range1Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
8% inhibition at 100 uM_first_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
9% inhibition at 50 uM_first_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
10% inhibition at 25 uM_first_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
11% inhibition at 12.5 uM_first_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
12% inhibition at 6.25 uM_first_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
13% inhibition at 3.125 uM_first_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
14% inhibition at 1.5625 uM_first_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
15% inhibition at 0.78125 uM_first_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
16% inhibition at 0.390625 uM_first_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
17% inhibition at 0.1953125 uM_first_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
18Excluded_Points_second_point_Range1Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
19% inhibition at 100 uM_second_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
20% inhibition at 50 uM_second_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
21% inhibition at 25 uM_second_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
22% inhibition at 12.5 uM_second_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
23% inhibition at 6.25 uM_second_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
24% inhibition at 3.125 uM_second_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
25% inhibition at 1.5625 uM_second_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
26% inhibition at 0.78125 uM_second_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
27% inhibition at 0.390625 uM_second_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
28% inhibition at 0.1953125 uM_second_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
29IC50_Qualifier_2_Range1This qualifier is to be used with the next TID, IC50_2_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
30IC50_2_Range1IC50 value determined using sigmoidal dose response equationFloatμM
31Std.Err(IC50)_2_Range1Standard Error of IC50 valueFloatμM
32nH_2_Range1Hill coefficient determined using sigmoidal dose response equationFloat
33Excluded_Points_third_point_Range1Flags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
34% inhibition at 100 uM_third_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
35% inhibition at 50 uM_third_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
36% inhibition at 25 uM_third_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
37% inhibition at 12.5 uM_third_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
38% inhibition at 6.25 uM_third_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
39% inhibition at 3.125 uM_third_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
40% inhibition at 1.5625 uM_third_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
41% inhibition at 0.78125 uM_third_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
42% inhibition at 0.390625 uM_third_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
43% inhibition at 0.1953125 uM_third_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
44Excluded_Points_fourth_point_Range1Flags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
45% inhibition at 100 uM_fourth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
46% inhibition at 50 uM_fourth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
47% inhibition at 25 uM_fourth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
48% inhibition at 12.5 uM_fourth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
49% inhibition at 6.25 uM_fourth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
50% inhibition at 3.125 uM_fourth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
51% inhibition at 1.5625 uM_fourth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
52% inhibition at 0.78125 uM_fourth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
53% inhibition at 0.390625 uM_fourth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
54% inhibition at 0.1953125 uM_fourth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
55IC50_Qualifier_3_Range1This qualifier is to be used with the next TID, IC50_3_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
56IC50_3_Range1IC50 value determined using sigmoidal dose response equationFloatμM
57Std.Err(IC50)_3_Range1Standard Error of IC50 valueFloatμM
58nH_3_Range1Hill coefficient determined using sigmoidal dose response equationFloat
59Excluded_Points_fifth_point_Range1Flags to indicate which of the fifth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
60% inhibition at 100 uM_fifth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
61% inhibition at 50 uM_fifth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
62% inhibition at 25 uM_fifth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
63% inhibition at 12.5 uM_fifth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
64% inhibition at 6.25 uM_fifth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
65% inhibition at 3.125 uM_fifth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
66% inhibition at 1.5625 uM_fifth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
67% inhibition at 0.78125 uM_fifth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
68% inhibition at 0.390625 uM_fifth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
69% inhibition at 0.1953125 uM_fifth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
70Excluded_Points_sixth_point_Range1Flags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
71% inhibition at 100 uM_sixth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
72% inhibition at 50 uM_sixth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
73% inhibition at 25 uM_sixth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
74% inhibition at 12.5 uM_sixth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
75% inhibition at 6.25 uM_sixth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
76% inhibition at 3.125 uM_sixth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
77% inhibition at 1.5625 uM_sixth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
78% inhibition at 0.78125 uM_sixth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
79% inhibition at 0.390625 uM_sixth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
80% inhibition at 0.1953125 uM_sixth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
81IC50_Qualifier_4_Range1This qualifier is to be used with the next TID, IC50_4_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
82IC50_4_Range1IC50 value determined using sigmoidal dose response equationFloatμM
83Std.Err(IC50)_4_Range1Hill coefficient determined using sigmoidal dose response equationFloatμM
84nH_4_Range1Hill coefficient determined using sigmoidal dose response equationFloat
85Excluded_Points_seventh_point_Range1Flags to indicate which of the seventh dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
86% inhibition at 100 uM_seventh_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
87% inhibition at 50 uM_seventh_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
88% inhibition at 25 uM_seventh_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
89% inhibition at 12.5 uM_seventh_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
90% inhibition at 6.25 uM_seventh_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
91% inhibition at 3.125 uM_seventh_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
92% inhibition at 1.5625 uM_seventh_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
93% inhibition at 0.78125 uM_seventh_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
94% inhibition at 0.390625 uM_seventh_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
95% inhibition at 0.1953125 uM_seventh_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
96Excluded_Points_eighth_point_Range1Flags to indicate which of the eighth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
97% inhibition at 100 uM_eighth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
98% inhibition at 50 uM_eighth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
99% inhibition at 25 uM_eighth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
100% inhibition at 12.5 uM_eighth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
101% inhibition at 6.25 uM_eighth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
102% inhibition at 3.125 uM_eighth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
103% inhibition at 1.5625 uM_eighth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
104% inhibition at 0.78125 uM_eighth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
105% inhibition at 0.390625 uM_eighth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
106% inhibition at 0.1953125 uM_eighth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
107IC50_Qualifier_5_Range1This qualifier is to be used with the next TID, IC50_5_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
108IC50_5_Range1IC50 value determined using sigmoidal dose response equationFloatμM
109Std.Err(IC50)_5_Range1Hill coefficient determined using sigmoidal dose response equationFloatμM
110nH_5_Range1Hill coefficient determined using sigmoidal dose response equationFloat
111Excluded_Points_ninth_point_Range1Flags to indicate which of the ninth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
112% inhibition at 100 uM_ninth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
113% inhibition at 50 uM_ninth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
114% inhibition at 25 uM_ninth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
115% inhibition at 12.5 uM_ninth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
116% inhibition at 6.25 uM_ninth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
117% inhibition at 3.125 uM_ninth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
118% inhibition at 1.5625 uM_ninth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
119% inhibition at 0.78125 uM_ninth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
120% inhibition at 0.390625 uM_ninth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
121% inhibition at 0.1953125 uM_ninth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
122Excluded_Points_tenth_point_Range1Flags to indicate which of the tenth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
123% inhibition at 100 uM_tenth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
124% inhibition at 50 uM_tenth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
125% inhibition at 25 uM_tenth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
126% inhibition at 12.5 uM_tenth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
127% inhibition at 6.25 uM_tenth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
128% inhibition at 3.125 uM_tenth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
129% inhibition at 1.5625 uM_tenth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
130% inhibition at 0.78125 uM_tenth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
131% inhibition at 0.390625 uM_tenth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
132% inhibition at 0.1953125 uM_tenth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
133IC50_Qualifier_1_Range2This qualifier is to be used with the next TID, IC50_1_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
134IC50_1_Range2IC50 value determined using sigmoidal dose response equationFloatμM
135Std.Err(IC50)_1_Range2Hill coefficient determined using sigmoidal dose response equationFloatμM
136nH_1_Range2Hill coefficient determined using sigmoidal dose response equationFloat
137Excluded_Points_first_point_Range2Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
138% inhibition at 50 uM_first_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
139% inhibition at 25 uM_first_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
140% inhibition at 12.5 uM_first_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
141% inhibition at 6.25 uM_first_point_Range2 (6.25μM**)% inhibition at a given concentrationFloat%
142% inhibition at 3.125 uM_first_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
143% inhibition at 1.5625 uM_first_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
144% inhibition at 0.78125 uM_first_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
145% inhibition at 0.390625 uM_first_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
146% inhibition at 0.1953125 uM_first_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
147% inhibition at 0.09765625 uM_first_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
148Excluded_Points_second_point_Range2Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
149% inhibition at 50 uM_second_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
150% inhibition at 25 uM_second_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
151% inhibition at 12.5 uM_second_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
152% inhibition at 6.25 uM_second_point_Range2 (6.25μM**)% inhibition at a given concentrationFloat%
153% inhibition at 3.125 uM_second_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
154% inhibition at 1.5625 uM_second_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
155% inhibition at 0.78125 uM_second_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
156% inhibition at 0.390625 uM_second_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
157% inhibition at 0.1953125 uM_second_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
158% inhibition at 0.09765625 uM_second_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
159IC50_Qualifier_2_Range2This qualifier is to be used with the next TID, IC50_2_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
160IC50_2_Range2IC50 value determined using sigmoidal dose response equationFloatμM
161Std.Err(IC50)_2_Range2Hill coefficient determined using sigmoidal dose response equationFloatμM
162nH_2_Range2Hill coefficient determined using sigmoidal dose response equationFloat
163Excluded_Points_third_point_Range2Flags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
164% inhibition at 50 uM_third_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
165% inhibition at 25 uM_third_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
166% inhibition at 12.5 uM_third_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
167% inhibition at 6.25 uM_third_point_Range2 (6.25μM**)% inhibition at a given concentrationFloat%
168% inhibition at 3.125 uM_third_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
169% inhibition at 1.5625 uM_third_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
170% inhibition at 0.78125 uM_third_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
171% inhibition at 0.390625 uM_third_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
172% inhibition at 0.1953125 uM_third_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
173% inhibition at 0.09765625 uM_third_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
174Excluded_Points_fourth_point_Range2Flags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
175% inhibition at 50 uM_fourth_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
176% inhibition at 25 uM_fourth_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
177% inhibition at 12.5 uM_fourth_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
178% inhibition at 6.25 uM_fourth_point_Range2 (6.26μM**)% inhibition at a given concentrationFloat%
179% inhibition at 3.125 uM_fourth_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
180% inhibition at 1.5625 uM_fourth_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
181% inhibition at 0.78125 uM_fourth_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
182% inhibition at 0.390625 uM_fourth_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
183% inhibition at 0.1953125 uM_fourth_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
184% inhibition at 0.09765625 uM_fourth_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
185IC50_Qualifier_3_Range2This qualifier is to be used with the next TID, IC50_3_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
186IC50_3_Range2IC50 value determined using sigmoidal dose response equationFloatμM
187Std.Err(IC50)_3_Range2Hill coefficient determined using sigmoidal dose response equationFloat
188nH_3_Range2Hill coefficient determined using sigmoidal dose response equationFloat
189Excluded_Points_fifth_point_Range2Flags to indicate which of the fifth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
190% inhibition at 50 uM_fifth_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
191% inhibition at 25 uM_fifth_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
192% inhibition at 12.5 uM_fifth_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
193% inhibition at 6.25 uM_fifth_point_Range2 (6.25μM**)% inhibition at a given concentrationFloat%
194% inhibition at 3.125 uM_fifth_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
195% inhibition at 1.5625 uM_fifth_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
196% inhibition at 0.78125 uM_fifth_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
197% inhibition at 0.390625 uM_fifth_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
198% inhibition at 0.1953125 uM_fifth_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
199% inhibition at 0.09765625 uM_fifth_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
200Excluded_Points_sixth_point_Range2Flags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
201% inhibition at 50 uM_sixth_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
202% inhibition at 25 uM_sixth_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
203% inhibition at 12.5 uM_sixth_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
204% inhibition at 6.25 uM_sixth_point_Range2 (6.25μM**)% inhibition at a given concentrationFloat%
205% inhibition at 3.125 uM_sixth_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
206% inhibition at 1.5625 uM_sixth_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
207% inhibition at 0.78125 uM_sixth_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
208% inhibition at 0.390625 uM_sixth_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
209% inhibition at 0.1953125 uM_sixth_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
210% inhibition at 0.09765625 uM_sixth_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
211IC50_Qualifier_4_Range2This qualifier is to be used with the next TID, IC50_4_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
212IC50_4_Range2IC50 value determined using sigmoidal dose response equationFloatμM
213Std.Err(IC50)_4_Range2Hill coefficient determined using sigmoidal dose response equationFloatμM
214nH_4_Range2Hill coefficient determined using sigmoidal dose response equationFloat
215Excluded_Points_seventh_point_Range2Flags to indicate which of the seventh dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
216% inhibition at 50 uM_seventh_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
217% inhibition at 25 uM_seventh_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
218% inhibition at 12.5 uM_seventh_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
219% inhibition at 6.25 uM_seventh_point_Range2 (6.25μM**)% inhibition at a given concentrationFloat%
220% inhibition at 3.125 uM_seventh_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
221% inhibition at 1.5625 uM_seventh_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
222% inhibition at 0.78125 uM_seventh_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
223% inhibition at 0.390625 uM_seventh_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
224% inhibition at 0.1953125 uM_seventh_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
225% inhibition at 0.09765625 uM_seventh_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
226Excluded_Points_eighth_point_Range2Flags to indicate which of the eighth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
227% inhibition at 50 uM_eighth_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
228% inhibition at 25 uM_eighth_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
229% inhibition at 12.5 uM_eighth_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
230% inhibition at 6.25 uM_eighth_point_Range2 (6.25μM**)% inhibition at a given concentrationFloat%
231% inhibition at 3.125 uM_eighth_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
232% inhibition at 1.5625 uM_eighth_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
233% inhibition at 0.78125 uM_eighth_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
234% inhibition at 0.390625 uM_eighth_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
235% inhibition at 0.1953125 uM_eighth_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
236% inhibition at 0.09765625 uM_eighth_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
237IC50_Qualifier_5_Range2This qualifier is to be used with the next TID, IC50_5_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
238IC50_5_Range2IC50 value determined using sigmoidal dose response equationFloatμM
239Std.Err(IC50)_5_Range2Hill coefficient determined using sigmoidal dose response equationFloatμM
240nH_5_Range2Hill coefficient determined using sigmoidal dose response equationFloat
241Excluded_Points_ninth_point_Range2Flags to indicate which of the ninth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
242% inhibition at 50 uM_ninth_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
243% inhibition at 25 uM_ninth_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
244% inhibition at 12.5 uM_ninth_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
245% inhibition at 6.25 uM_ninth_point_Range2 (6.25μM**)% inhibition at a given concentrationFloat%
246% inhibition at 3.125 uM_ninth_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
247% inhibition at 1.5625 uM_ninth_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
248% inhibition at 0.78125 uM_ninth_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
249% inhibition at 0.390625 uM_ninth_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
250% inhibition at 0.1953125 uM_ninth_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
251% inhibition at 0.09765625 uM_ninth_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
252Excluded_Points_tenth_point_Range2Flags to indicate which of the tenth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
253% inhibition at 50 uM_tenth_point_Range2 (50μM**)% inhibition at a given concentrationFloat%
254% inhibition at 25 uM_tenth_point_Range2 (25μM**)% inhibition at a given concentrationFloat%
255% inhibition at 12.5 uM_tenth_point_Range2 (12.5μM**)% inhibition at a given concentrationFloat%
256% inhibition at 6.25 uM_tenth_point_Range2 (6.25μM**)% inhibition at a given concentrationFloat%
257% inhibition at 3.125 uM_tenth_point_Range2 (3.125μM**)% inhibition at a given concentrationFloat%
258% inhibition at 1.5625 uM_tenth_point_Range2 (1.5625μM**)% inhibition at a given concentrationFloat%
259% inhibition at 0.78125 uM_tenth_point_Range2 (0.78125μM**)% inhibition at a given concentrationFloat%
260% inhibition at 0.390625 uM_tenth_point_Range2 (0.390625μM**)% inhibition at a given concentrationFloat%
261% inhibition at 0.1953125 uM_tenth_point_Range2 (0.195312μM**)% inhibition at a given concentrationFloat%
262% inhibition at 0.09765625 uM_tenth_point_Range2 (0.0976562μM**)% inhibition at a given concentrationFloat%
263IC50_Qualifier_1_Range3This qualifier is to be used with the next TID, IC50_1_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
264IC50_1_Range3IC50 value determined using sigmoidal dose response equationFloatμM
265Std.Err(IC50)_1_Range3Standard Error of IC50 valueFloatμM
266nH_1_Range3Hill coefficient determined using sigmoidal dose response equationFloat
267Excluded_Points_first_point_Range3Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
268% inhibition at 10 uM_first_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
269% inhibition at 5 uM_first_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
270% inhibition at 2.5 uM_first_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
271% inhibition at 1.25 uM_first_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
272% inhibition at 0.625 uM_first_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
273% inhibition at 0.3125 uM_first_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
274% inhibition at 0.15625 uM_first_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
275% inhibition at 0.078125 uM_first_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
276% inhibition at 0.0390625 uM_first_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
277% inhibition at 0.01953125 uM_first_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
278Excluded_Points_second_point_Range3Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
279% inhibition at 10 uM_second_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
280% inhibition at 5 uM_second_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
281% inhibition at 2.5 uM_second_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
282% inhibition at 1.25 uM_second_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
283% inhibition at 0.625 uM_second_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
284% inhibition at 0.3125 uM_second_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
285% inhibition at 0.15625 uM_second_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
286% inhibition at 0.078125 uM_second_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
287% inhibition at 0.0390625 uM_second_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
288% inhibition at 0.01953125 uM_second_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
289IC50_Qualifier_2_Range3This qualifier is to be used with the next TID, IC50_2_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
290IC50_2_Range3IC50 value determined using sigmoidal dose response equationFloatμM
291Std.Err(IC50)_2_Range3Standard Error of IC50 valueFloatμM
292nH_2_Range3Hill coefficient determined using sigmoidal dose response equationFloat
293Excluded_Points_third_point_Range3Flags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
294% inhibition at 10 uM_third_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
295% inhibition at 5 uM_third_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
296% inhibition at 2.5 uM_third_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
297% inhibition at 1.25 uM_third_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
298% inhibition at 0.625 uM_third_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
299% inhibition at 0.3125 uM_third_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
300% inhibition at 0.15625 uM_third_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
301% inhibition at 0.078125 uM_third_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
302% inhibition at 0.0390625 uM_third_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
303% inhibition at 0.01953125 uM_third_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
304Excluded_Points_fourth_point_Range3Flags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
305% inhibition at 10 uM_fourth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
306% inhibition at 5 uM_fourth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
307% inhibition at 2.5 uM_fourth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
308% inhibition at 1.25 uM_fourth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
309% inhibition at 0.625 uM_fourth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
310% inhibition at 0.3125 uM_fourth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
311% inhibition at 0.15625 uM_fourth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
312% inhibition at 0.078125 uM_fourth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
313% inhibition at 0.0390625 uM_fourth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
314% inhibition at 0.01953125 uM_fourth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
315IC50_Qualifier_1_Range4This qualifier is to be used with the next TID, IC50_1_Range4. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
316IC50_1_Range4IC50 value determined using sigmoidal dose response equationFloatμM
317Std.Err(IC50)_1_Range4Standard Error of IC50 valueFloatμM
318nH_1_Range4Hill coefficient determined using sigmoidal dose response equationFloat
319Excluded_Points_first_point_Range4Flags to indicate which of the fifth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
320% inhibition at 1 uM_first_point_Range4 (1μM**)% inhibition at a given concentrationFloat%
321% inhibition at 0.5 uM_first_point_Range4 (0.5μM**)% inhibition at a given concentrationFloat%
322% inhibition at 0.25 uM_first_point_Range4 (0.25μM**)% inhibition at a given concentrationFloat%
323% inhibition at 0.125 uM_first_point_Range4 (0.125μM**)% inhibition at a given concentrationFloat%
324% inhibition at 0.0625 uM_first_point_Range4 (0.0625μM**)% inhibition at a given concentrationFloat%
325% inhibition at 0.03125 uM_first_point_Range4 (0.03125μM**)% inhibition at a given concentrationFloat%
326% inhibition at 0.015625 uM_first_point_Range4 (0.015625μM**)% inhibition at a given concentrationFloat%
327% inhibition at 0.0078125 uM_first_point_Range4 (0.0078125μM**)% inhibition at a given concentrationFloat%
328% inhibition at 0.00390625 uM_first_point_Range4 (0.00390625μM**)% inhibition at a given concentrationFloat%
329% inhibition at 0.001953125 uM_first_point_Range4 (0.00195312μM**)% inhibition at a given concentrationFloat%
330Excluded_Points_second_point_Range4Flags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
331% inhibition at 1 uM_second_point_Range4 (1μM**)% inhibition at a given concentrationFloat%
332% inhibition at 0.5 uM_second_point_Range4 (0.5μM**)% inhibition at a given concentrationFloat%
333% inhibition at 0.25 uM_second_point_Range4 (0.25μM**)% inhibition at a given concentrationFloat%
334% inhibition at 0.125 uM_second_point_Range4 (0.125μM**)% inhibition at a given concentrationFloat%
335% inhibition at 0.0625 uM_second_point_Range4 (0.0625μM**)% inhibition at a given concentrationFloat%
336% inhibition at 0.03125 uM_second_point_Range4 (0.03125μM**)% inhibition at a given concentrationFloat%
337% inhibition at 0.015625 uM_second_point_Range4 (0.015625μM**)% inhibition at a given concentrationFloat%
338% inhibition at 0.0078125 uM_second_point_Range4 (0.0078125μM**)% inhibition at a given concentrationFloat%
339% inhibition at 0.00390625 uM_second_point_Range4 (0.00390625μM**)% inhibition at a given concentrationFloat%
340% inhibition at 0.001953125 uM_second_point_Range4 (0.00195312μM**)% inhibition at a given concentrationFloat%
341IC50_Qualifier_2_Range4This qualifier is to be used with the next TID, IC50_2_Range4. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
342IC50_2_Range4IC50 value determined using sigmoidal dose response equationFloatμM
343Std.Err(IC50)_2_Range4Standard Error of IC50 valueFloatμM
344nH_2_Range4Hill coefficient determined using sigmoidal dose response equationFloat
345Excluded_Points_third_point_Range4Flags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
346% inhibition at 1 uM_third_point_Range4 (1μM**)% inhibition at a given concentrationFloat%
347% inhibition at 0.5 uM_third_point_Range4 (0.5μM**)% inhibition at a given concentrationFloat%
348% inhibition at 0.25 uM_third_point_Range4 (0.25μM**)% inhibition at a given concentrationFloat%
349% inhibition at 0.125 uM_third_point_Range4 (0.125μM**)% inhibition at a given concentrationFloat%
350% inhibition at 0.0625 uM_third_point_Range4 (0.0625μM**)% inhibition at a given concentrationFloat%
351% inhibition at 0.03125 uM_third_point_Range4 (0.03125μM**)% inhibition at a given concentrationFloat%
352% inhibition at 0.015625 uM_third_point_Range4 (0.015625μM**)% inhibition at a given concentrationFloat%
353% inhibition at 0.0078125 uM_third_point_Range4 (0.0078125μM**)% inhibition at a given concentrationFloat%
354% inhibition at 0.00390625 uM_third_point_Range4 (0.00390625μM**)% inhibition at a given concentrationFloat%
355% inhibition at 0.001953125 uM_third_point_Range4 (0.00195312μM**)% inhibition at a given concentrationFloat%
356Excluded_Points_fourth_point_Range4Flags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
357% inhibition at 1 uM_fourth_point_Range4 (1μM**)% inhibition at a given concentrationFloat%
358% inhibition at 0.5 uM_fourth_point_Range4 (0.5μM**)% inhibition at a given concentrationFloat%
359% inhibition at 0.25 uM_fourth_point_Range4 (0.25μM**)% inhibition at a given concentrationFloat%
360% inhibition at 0.125 uM_fourth_point_Range4 (0.125μM**)% inhibition at a given concentrationFloat%
361% inhibition at 0.0625 uM_fourth_point_Range4 (0.0625μM**)% inhibition at a given concentrationFloat%
362% inhibition at 0.03125 uM_fourth_point_Range4 (0.03125μM**)% inhibition at a given concentrationFloat%
363% inhibition at 0.015625 uM_fourth_point_Range4 (0.015625μM**)% inhibition at a given concentrationFloat%
364% inhibition at 0.0078125 uM_fourth_point_Range4 (0.0078125μM**)% inhibition at a given concentrationFloat%
365% inhibition at 0.00390625 uM_fourth_point_Range4 (0.00390625μM**)% inhibition at a given concentrationFloat%
366% inhibition at 0.001953125 uM_fourth_point_Range4 (0.00195312μM**)% inhibition at a given concentrationFloat%

* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: MH084230-01A1

Data Table (Concise)
Classification
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