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BioAssay: AID 179

NCI AIDS Antiviral Assay

The ability of compounds to protect human CEM cells from HIV-1 infection is measured as a screen for new compounds capable of inhibiting the HIV virus. Five concentrations of drug were tested on uninfected and infected cells and cell growth was measured using a soluble formazan assay. The dose response curve for the uninfected cells was used to calculate an IC50, the concentration of drug that more ..
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 Tested Compounds
 Tested Compounds
All(41977)
 
 
Active(1529)
 
 
Inactive(40181)
 
 
Unspecified(290)
 
 
 Tested Substances
 Tested Substances
All(44150)
 
 
Active(1812)
 
 
Inactive(42038)
 
 
Unspecified(300)
 
 
 Related BioAssays
 Related BioAssays
AID: 179
Data Source: DTP/NCI
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Deposit Date: 2004-08-15
Modify Date: 2005-08-11

Data Table ( Complete ):           View Active Data    View All Data
BioActive Compounds: 1529
Description:
The ability of compounds to protect human CEM cells from HIV-1 infection is measured as a screen for new compounds capable of inhibiting the HIV virus. Five concentrations of drug were tested on uninfected and infected cells and cell growth was measured using a soluble formazan assay. The dose response curve for the uninfected cells was used to calculate an IC50, the concentration of drug that causes 50% inhibition of growth (a measure of toxicity). The dose respose curve for the infected cells was used to calculate an EC50, the concentration of drug that provides 50% protection from the growth inhibition caused by the HIV virus. Compounds that provided at least 50% protection were retested. Compounds that provided at least 50% protection on retest were listed as moderately active. Compounds that reproducibly provided 100% protection were listed as confirmed active. COmpounds not meeting these criteria were listed as confirmed inactive. Note that the inability to provide protection could be due to toxicity.
Comment
Compounds are identified by the NCI NSC number.
Compounds that provided at least 50% protection on retest were considered as active. Activity score was based on increasing values of -LogEC50 (unit M).
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1logec50Log of the EC50 result, unit: M.FloatM
2logec50Log of the EC50 result, unit: ug/mL.Floatug/mL
3logec50Log of the EC50 result, unit: Volumetric.Floatv
4indnec50Number of tests averaged for the EC50 value for this NSC.Integer
5stddevec50Standard Deviation of the Log10 of the EC50 result averaged across all tests for this NSC.Float
6logic50Log of the IC50 result, unit: M.FloatM
7logic50Log of the IC50 result, unit: ug/mL.Floatug/mL
8logic50Log of the IC50 result, unit: Volumetric.Floatv
9indnic50Number of tests averaged for the IC50 value for this NSC.Integer
10stddevic50Standard Deviation of the Log10 of the IC50 result averaged across all tests for this NSC.Float

Data Table (Concise)
Data Table ( Complete ):     View Active Data    View All Data
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