The microtubule-associated protein tau is an abundant protein in the axons of neurons that stabilizes microtubules. With its ability to modulate microtubule dynamics, tau contributes directly or indirectly, to key structural and regulatory cellular functions. Particularly important is the influence tau exerts on axonal transport, which allows signaling molecules, trophic factors and other more ..
Related BioAssays
Related BioAssays
Target
BioActive Compounds: 31
Depositor Specified Assays
| AID | Name | Type | Comment |
|---|---|---|---|
| 1460 | qHTS for Inhibitors of Tau Fibril Formation, Thioflavin T Binding | confirmatory | |
| 1468 | qHTS for Inhibitors of Tau Fibril Formation, Fluorescence Polarization | confirmatory | |
| 1558 | Confirmation Concentration-Response Assay for for Inhibitors of Tau Fibril Formation, Thioflavin T Binding | confirmatory | |
| 1475 | Quantitative High-Throughput Screen for Inhibitors of Tau Fibril Formation: Summary | summary |
Description:
NIH Molecular Libraries Probe Production Network [MLPCN]
NIH Chemical Genomics Center [NCGC]
MLPCN Grant: X01 MH083262-01
Assay Provider: Carlo Ballatore, University of Pennsylvania
NCGC Assay Overview:
The microtubule-associated protein tau is an abundant protein in the axons of neurons that stabilizes microtubules. With its ability to modulate microtubule dynamics, tau contributes directly or indirectly, to key structural and regulatory cellular functions. Particularly important is the influence tau exerts on axonal transport, which allows signaling molecules, trophic factors and other essential cellular constituents to travel along the axons. Under pathological conditions, tau becomes sequestered into insoluble aggregates called neurofibrillary tangles. This phenomenon is believed to have pathological consequences by promoting axonal transport deficits that ultimately lead to synaptic dysfunction and neuronal loss. The ability of compounds to block tau fibrillization was visualized using transmission electron microscopy (TEM). Samples from the tau ThT confirmation assay treated with 100 uM compound (AID 1558) were centrifuged and imaged using TEM. Electron micrographs of representative fields of objects were imaged to judge fibril content using grading scale from 0-4: 0, all fibrils in clumps, no fragments; 1, small fibril clumps, free filaments and some pieces; 2, filaments and pieces but no clumps; 3, short filaments and tiny pieces; 4, only tiny pieces.
Crowe, A., Ballatore, C., Hyde, E., Trojanowski, J. Q., and Lee, V. M. Y. (2007) Biochemical and Biophysical Research Communications 358, 1-6.
NIH Chemical Genomics Center [NCGC]
MLPCN Grant: X01 MH083262-01
Assay Provider: Carlo Ballatore, University of Pennsylvania
NCGC Assay Overview:
The microtubule-associated protein tau is an abundant protein in the axons of neurons that stabilizes microtubules. With its ability to modulate microtubule dynamics, tau contributes directly or indirectly, to key structural and regulatory cellular functions. Particularly important is the influence tau exerts on axonal transport, which allows signaling molecules, trophic factors and other essential cellular constituents to travel along the axons. Under pathological conditions, tau becomes sequestered into insoluble aggregates called neurofibrillary tangles. This phenomenon is believed to have pathological consequences by promoting axonal transport deficits that ultimately lead to synaptic dysfunction and neuronal loss. The ability of compounds to block tau fibrillization was visualized using transmission electron microscopy (TEM). Samples from the tau ThT confirmation assay treated with 100 uM compound (AID 1558) were centrifuged and imaged using TEM. Electron micrographs of representative fields of objects were imaged to judge fibril content using grading scale from 0-4: 0, all fibrils in clumps, no fragments; 1, small fibril clumps, free filaments and some pieces; 2, filaments and pieces but no clumps; 3, short filaments and tiny pieces; 4, only tiny pieces.
Crowe, A., Ballatore, C., Hyde, E., Trojanowski, J. Q., and Lee, V. M. Y. (2007) Biochemical and Biophysical Research Communications 358, 1-6.
Protocol
NCGC Assay Protocol Summary:
Human K18 P301L tau (15 uM) in assay buffer (20 uM heparin, 12.5 uM Thioflavine T, 100 mM sodium acetate pH 7) was centrifuged at 186,000 g for 30 min and the supernatant removed from the pellet. Pellets were resuspended, stained with uranyl acetate, and imaged by electron microscopy.
Human K18 P301L tau (15 uM) in assay buffer (20 uM heparin, 12.5 uM Thioflavine T, 100 mM sodium acetate pH 7) was centrifuged at 186,000 g for 30 min and the supernatant removed from the pellet. Pellets were resuspended, stained with uranyl acetate, and imaged by electron microscopy.
Comment
Compound Ranking:
Compounds are scored based on their fibril content grade. The actual grade value times 20 is used as the score for active compounds. Inactive compounds are assigned a score of 0. Inconclusive compounds are assigned a score of 20.
Compounds are scored based on their fibril content grade. The actual grade value times 20 is used as the score for active compounds. Inactive compounds are assigned a score of 0. Inconclusive compounds are assigned a score of 20.
Result Definitions
| TID | Name | Description | Histogram | Type | Unit | |
|---|---|---|---|---|---|---|
| Outcome | The BioAssay activity outcome | Outcome | ||||
| Score | The BioAssay activity ranking score |  | Integer | |||
| 1 | Phenotype | Indicates type of activity observed: inhibitor, activator, fluorescent, cytotoxic, inactive, or inconclusive. | String | |||
| 2 | Fibril Content Grade | Score assigned to compound based on electron micrograph images of representative fields of objects that show the tau protein fibril content after exposure to compound. | | Float | ||
| 3 | Compound QC | NCGC designation for data stage: 'qHTS', 'qHTS Verification', 'Secondary Profiling' | String |
Additional Information
Grant Number: X01 MH083262-01
Data Table (Concise)
Classification
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