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BioAssay: AID 1658

Summary Assay for the inhibition of Hepatic Lipid Droplet Formation

Medical researchers world-wide now use the term "epidemic" or "pandemic" to describe the alarming incidence of obesity in modern society, which is estimated to occur in 30% of the general US population. Perhaps, most alarming is the high incidence of obesity in children and adolescents, which indicates that medical problems associated with obesity (e.g., diabetes, metabolic syndrome, and heart more ..
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 Related BioAssays
 Related BioAssays
AID: 1658
Data Source: Burnham Center for Chemical Genomics (BCCG-A184-Lipid-Droplet-Imaging-Summary-Assay)
BioAssay Type: Summary, Candidate Probes/Leads with Supporting Evidence
Depositor Category: NIH Molecular Libraries Probe Production Network
BioAssay Version:
Deposit Date: 2009-04-03
Modify Date: 2011-03-16
Depositor Specified Assays
Show more
AIDNameTypeComment
1656High Throughput Imaging Assay for Hepatic Lipid Droplet FormationscreeningPrimary Screen
493092SAR Analysis for the identification of small molecules that inhibit hepatic lipid droplet formation using an image based screen.confirmatory
504422SAR Analysis for the identification of small molecules that inhibit hepatic lipid droplet formation in human hepatocytes using an image based screen.confirmatory
504440SAR Analysis for the identification of small molecules that inhibit hepatic lipid droplet formation using an image based screen - Set 2confirmatory
504443SAR analysis of compound cytotoxicity in hepatocytes (AML12 cells)confirmatory
463183Single concentration confirmation of HCS identification of small molecules that inhibit hepatic lipid droplet formationscreening
463191Dose response confirmation of HCS identification of small molecules that inhibit hepatic lipid droplet formation.confirmatory
463200HTS Counterscreen for Detection of Compound Cytotoxicity in Hepatocytes (AML12 cells)screening
463219Single concentration confirmation of compound cytotoxicity in hepatocytes (AML12 cells).screening
Description:
Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG)
Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA)
Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN)
Grant Proposal Number: 1 R03 MH083261-01A1
Assay Provider: Dr. Patrick M. McDonough, Vala Sciences Inc.

Medical researchers world-wide now use the term "epidemic" or "pandemic" to describe the alarming incidence of obesity in modern society, which is estimated to occur in 30% of the general US population. Perhaps, most alarming is the high incidence of obesity in children and adolescents, which indicates that medical problems associated with obesity (e.g., diabetes, metabolic syndrome, and heart disease) will increase in the foreseeable future, as obesity often increases with age. The dominant cellular basis for obesity is increased accumulation of triglycerides in lipid droplets within the cell. The two major cell types in which lipid droplet formation leads to pathological problems are adipocytes and hepatocytes.

The overall goal of this project is to identify chemical probes for inhibiting lipid droplet formation in hepatocytes. Identification of such compounds will better our understanding of the biochemical/molecular pathways that lead to the development of fatty liver disease, and may identify lead compounds for consideration for therapeutic development.

References:
1) Hill, J. O., Peters, J. C., Wyatt, H. R. 2007. The role of public policy in treating the epidemic of global obesity, Clin Pharmacol Ther. 2007 May;81(5):772-5.

2) Fujimoto, Y., Itabe, H., Kinoshita, T., Homma, K. J., Onoduka, et al. 2007, Involvement of ACSL in local synthesis of neutral lipids in cytoplasmic lipid droplets in human hepatocyte HuH7. J. Lipid Res. 48:1280-1292

3) Reddy, J. K., Rao, M. S. 2006. Lipid metabolism and liver inflammation. II. Fatty liver disease and fatty acid oxidation, Am. J. Phsyiol. Gastrointest. Liver Physiol. 290:G852-G858
Protocol
Please see pertinent AID: 1656, 493092, 504422, 504440, 504443
Comment
This AID is used to summarize the results of this MLPCN project. Thus far the work reported has not reached the point where probes have been identified. Probe molecules are defined as the positives of this assay and assigned a score of 100
Additional Information
Grant Number: 1 R03 MH083261-01A1

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