Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in most organisms. In human, four isozymes of APs have been identified: three isozymes are tissue-specific and the fourth one is tissue-nonspecific. Placental alkaline phosphatase (PLAP) is highly expressed in primate placental tissue. Its biological function is unknown. Identification of PLAP-specific inhibitors should provide necessary tools for characterization of its biological role. ..more
Target
Depositor Specified Assays
| AID | Name | Type | Comment |
|---|---|---|---|
| 518 | TNAP luminescent HTS assay | confirmatory | TNAP luminescent HTS assay |
| 690 | Luminescent assay for HTS discovery of chemical inhibitors of placental alkaline phosphatase | confirmatory | Luminescent assay for HTS discovery of chemical inhibitors of placental alkaline phosphatase |
| 696 | Luminescent assay for HTS discovery of chemical activators of placental alkaline phosphatase | confirmatory | Luminescent assay for HTS discovery of chemical activators of placental alkaline phosphatase |
| 1017 | Luminescent assay for identification of inhibitors of human intestinal alkaline phosphatase | confirmatory | Luminescent assay for identification of inhibitors of human intestinal alkaline phosphatase |
| 1019 | Luminescent assay for identification of inhibitors of bovine intestinal alkaline phosphatase | screening | Luminescent assay for identification of inhibitors of bovine intestinal alkaline phosphatase |
| 1056 | SAR analysis of an In Vitro TNAP Dose Response Luminescent Assay | confirmatory | In Vitro TNAP Dose Response Luminescent Assay for SAR Study |
| 1512 | Luminescent assay for HTS discovery of chemical inhibitors of placental alkaline phosphatase confirmation | confirmatory | Luminescent assay for HTS discovery of chemical inhibitors of placental alkaline phosphatase confirmation |
Description:
Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG)
Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA)
Network: NIH Molecular Libraries Screening Centers Network (MLSCN)
Grant Proposal Number: MH077602-01
Assay Provider: Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA.
Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in most organisms. In human, four isozymes of APs have been identified: three isozymes are tissue-specific and the fourth one is tissue-nonspecific. Placental alkaline phosphatase (PLAP) is highly expressed in primate placental tissue. Its biological function is unknown. Identification of PLAP-specific inhibitors should provide necessary tools for characterization of its biological role.
PLAP screening was developed and performed at the Sanford-Burnham Center for Chemical Genomics (SBCCG) as part of the Molecular Library Screening Center Network (MLSCN). AID 690 was a selectivity screening for tissue nonspecific alkaline phosphatase (TNAP) screened at SBCCG (AID 518). XO1 submission, MH077602-01, Pharmacological inhibitors of tissue-nonspecific alkaline phosphatase (TNAP), Assay Provider Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA.
AIDs 518, 690, 696, 1017, 1019 and 1512 were carried out to select probe molecules SID-56373725(ML085) and SID-56405584(ML095).
Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA)
Network: NIH Molecular Libraries Screening Centers Network (MLSCN)
Grant Proposal Number: MH077602-01
Assay Provider: Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA.
Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in most organisms. In human, four isozymes of APs have been identified: three isozymes are tissue-specific and the fourth one is tissue-nonspecific. Placental alkaline phosphatase (PLAP) is highly expressed in primate placental tissue. Its biological function is unknown. Identification of PLAP-specific inhibitors should provide necessary tools for characterization of its biological role.
PLAP screening was developed and performed at the Sanford-Burnham Center for Chemical Genomics (SBCCG) as part of the Molecular Library Screening Center Network (MLSCN). AID 690 was a selectivity screening for tissue nonspecific alkaline phosphatase (TNAP) screened at SBCCG (AID 518). XO1 submission, MH077602-01, Pharmacological inhibitors of tissue-nonspecific alkaline phosphatase (TNAP), Assay Provider Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA.
AIDs 518, 690, 696, 1017, 1019 and 1512 were carried out to select probe molecules SID-56373725(ML085) and SID-56405584(ML095).
Protocol
Please see pertinent AIDs: 518, 690, 696, 1017, 1019, 1056, 1512
Comment
Probe molecules are defined as the positives of this assay and assigned a score of 100
Result Definitions
| TID | Name | Description | Histogram | Type | Unit | |
|---|---|---|---|---|---|---|
| Outcome | The BioAssay activity outcome | Outcome | ||||
| Score | The BioAssay activity ranking score |  | Integer | |||
| 1 | ML# | ML# for the compound | String |
Additional Information
Grant Number: MH081277-01
Data Table (Concise)
Classification
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