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BioAssay: AID 1399

Dose response cell-based assay to measure STAT3 inhibition

Members of the signal transducer and activator of transcription (STAT) family of transcription factors mediate inflammation, cell survival, differentiation, and proliferation (1, 2). In response to stimuli such as growth factors and cytokines (1-3), cytosolic STATs are activated by phosphorylation by the Janus-activated kinases (Jaks), inducing STAT dimerization, nuclear translocation, and more ..
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 Tested Compounds
 Tested Compounds
All(118)
 
 
Active(116)
 
 
Inactive(2)
 
 
 Tested Substances
 Tested Substances
All(118)
 
 
Active(116)
 
 
Inactive(2)
 
 
AID: 1399
Data Source: The Scripps Research Institute Molecular Screening Center (STAT3_INH_LUMI_1536_IC50)
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Depositor Category: NIH Molecular Libraries Probe Production Network
Deposit Date: 2008-09-25

Data Table ( Complete ):           Active    All
Target
BioActive Compounds: 116
Depositor Specified Assays
AIDNameTypeProbeComment
1265Confirmation cell-based high throughput screening assay to measure STAT3 inhibitionscreening
1308Counterscreen assay for STAT3 inhibitors: Cell-based high throughput assay to measure NF-kappaB inhibition.screening
1317Counterscreen assay for STAT3 inhibitors: Cell-based high throughput assay to measure STAT1 inhibitionscreening
862Primary cell-based high throughput screening assay to measure STAT3 inhibitionscreening
920Primary cell-based high throughput screening assay to measure STAT1 inhibitionscreening
1806Summary of probe development efforts to identify inhibitors of signal transducer and activator of transcription 3 (STAT3)summary2
2078Late stage results from the probe development efforts to identify inhibitors of signal transducer and activator of transcription 3 (STAT3).screening
Description:
Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center
Center Affiliation: The Scripps Research Institute (TSRI)
Assay Provider: David Frank, Dana Farber Cancer Institute
Network: Molecular Libraries Probe Production Centers Network (MLPCN)
Grant Proposal Number: 1 X01 MH079826-01
Grant Proposal PI: David Frank, Dana Farber Cancer Institute

External Assay ID: STAT3_INH_LUMI_1536_IC50

Name: Dose response cell-based assay to measure STAT3 inhibition

Description:

Members of the signal transducer and activator of transcription (STAT) family of transcription factors mediate inflammation, cell survival, differentiation, and proliferation (1, 2). In response to stimuli such as growth factors and cytokines (1-3), cytosolic STATs are activated by phosphorylation by the Janus-activated kinases (Jaks), inducing STAT dimerization, nuclear translocation, and binding to specific enhancer elements in target genes (2). Although structurally similar, STAT proteins possess diverse biological roles (2). For example, STAT1 activity is pro-inflammatory, anti-proliferative and mediates the effects of IFN-gamma, while STAT3 activity is anti-inflammatory, pro-apoptotic, and mediates IL-6 signaling (2, 4). Studies showing that STAT3 is activated in breast and prostate cancers, that genetic inhibition of STAT3 reduces cell proliferation, survival, and wound healing (1, 4, 5), and that disrupting STAT3-EGFR interactions reduces tumor growth (6), suggest that STAT3 activation has broad cellular effects. As a result, the identification of selective STAT3 modulators may provide useful tools for exploring STAT3 biology.


References:

1. Alvarez JV, Febbo PG, Ramaswamy S, Loda M, Richardson A, Frank DA. Identification of a genetic signature of activated signal transducer and activator of transcription 3 in human tumors. Cancer Res. 2005 Jun 15;65(12):5054-62.
2. Schindler C, Levy DE, Decker T. JAK-STAT signaling: from interferons to cytokines. J Biol Chem. 2007 Jul 13;282(28):20059-63.
3. Germain D, Frank DA. Targeting the cytoplasmic and nuclear functions of signal transducers and activators of transcription 3 for cancer therapy. Clin Cancer Res. 2007 Oct 1;13(19):5665-9.
4. Levy DE, Darnell JE Jr. Stats: transcriptional control and biological impact. Nat Rev Mol Cell Biol. 2002 Sep;3(9):651-62.
5. Battle TE, Wierda WG, Rassenti LZ, Zahrieh D, Neuberg D, Kipps TJ, Frank DA. In vivo activation of signal transducer and activator of transcription 1 after CD154 gene therapy for chronic lymphocytic leukemia is associated with clinical and immunologic response. Clin Cancer Res. 2003 Jun;9(6):2166-72.
6. Takeda K, Kaisho T, Yoshida N, Takeda J, Kishimoto T, Akira S.1998. Stat3 activation is responsible for IL-6-dependent T cell proliferation through preventing apoptosis: generation and characterization of T cell-specific Stat3-deficient mice. J. Immunol. 161:4652-4660.

Keywords:

STAT3, acute-phase response factor, APRF, transcription factor, HTS, assay, inhibition, inhibitor, dose response, luciferase, luminescence, reporter, 1536, Scripps, Scripps Research Institute Molecular Screening Center, SRIMSC, Molecular Libraries Probe Production Centers Network, MLPCN.
Protocol
Assay Overview:

The purpose of this assay is to determine dose response curves for compounds identified as active in a previous set of experiments entitled, "Primary cell-based high throughput screening assay to measure STAT3 inhibition" (PubChem AID 862), and that confirmed activity in a set of experiments entitled, "Confirmation cell-based high throughput screening assay to measure STAT3 inhibition" (AID 1265). The compounds selected for testing in this AID met the following criteria: 1) they were declared active in AID 862 and AID 1265; 2) they were declared inactive in a set of experiments entitled, "Primary cell-based high throughput screening assay to measure STAT1 inhibition" (AID 920); 3) they were inactive in a set of experiments entitled, "Counterscreen assay for STAT3 inhibitors: cell-based high throughput assay to measure NF-kappaB inhibition" (AID 1308); and 4) they were inactive in a set of experiments entitled, "Counterscreen assay for STAT3 inhibitors: cell-based high throughput assay to measure STAT1 inhibition" (AID 1317).

In this assay STAT3 inhibition was measured using a human U3A fibrosarcoma cell line that stably expresses a human STAT3::luciferase construct. This cell line is deficient in STAT1. Test compounds were screened for their ability to prevent or reduce IL-6-mediated STAT3::luciferase reporter activity. Cells were exposed to test compounds, followed by treatment with IL-6 to activate STAT3 transcription. Changes in STAT3::luciferase activity were monitored by measuring well luminescence. As designed, a STAT3 inhibitor will block IL-6-mediated STAT3 transcription, thus reducing transcription of the luciferase reporter gene, leading to decreased well luminescence. Compounds were tested in triplicate using a 10-point, 1:3 dilution series, starting at a nominal test concentration of 55.7 uM.

Protocol Summary:

The inhibitor and activator dose response assays using STAT3::luciferase cells were run simultaneously. U3A cells were grown in T-175 flasks in Dulbecco's Modified Eagle's Media (DMEM) supplemented with 10% v/v fetal bovine serum and antibiotics (50 ug/ml each of penicillin and streptomycin, 100 ug/mL neomycin) at 37 degrees C in an atmosphere of 5% CO2 and 95% relative humidity (RH).

Prior to the start of the assay the cells were resuspended at a density of 1.88 million cells/mL in phenol red-free growth medium, and filtered through a 0.7 micron filter. Next, 4 ul of cell suspension (7,520 cells per well) were dispensed into each well of 1536-well plates. The assay was started by immediately dispensing 28 nL of test compound in DMSO, nifuroxazide (112 uM final nominal concentration, set as 100% inhibition) in DMSO, or DMSO alone (0.6% final concentration) to the appropriate wells. The plates were then incubated for 1 hour at 37 degrees C (5% CO2, 95% RH). Next, 1 ul of human recombinant IL-6 (33 ng/ml final nominal EC80 concentration) was dispensed into all wells. The plates then were incubated for 6 hours at 37 degrees C (5% CO2, 95% RH). The assay was stopped by dispensing 5 ul of SteadyLite HTS luciferase substrate at room temperature to each well, followed by incubation at room temperature for 15 minutes. Well luminescence was measured on the ViewLux plate reader.

The percent inhibition was defined using the following mathematical formula:

% Inhibition = 100[1 - ((Test_Compound - Median_High_Control) / (Median_Low_Control - Median_High_Control))]

Where:
Test_Compound is defined as the luminescence value of a well containing IL-6 and test compound.

Median_High_Control is defined as the median luminescence of wells containing IL-6 and nifuroxazide.

Median_Low_Control is defined as the median luminescence of wells containing IL-6 and DMSO.

For each test compound, percent inhibition was plotted against compound concentration. A four parameter equation describing a sigmoidal dose-response curve was then fitted with adjustable baseline using Assay Explorer software (MDL Information Systems). The reported IC50 values were generated from fitted curves by solving for the X-intercept value at the 50% inhibition level of the Y-intercept value. In cases where the highest concentration tested (i.e. 55.7 uM) did not result in greater than 50% inhibition, the IC50 was determined manually as greater than 55.7 uM. Compounds with an IC50 greater than 10 uM were considered inactive. Compounds with an IC50 equal to or less than 10 uM were considered active.

Any compound with a percent inhibition value <50% at all test concentrations was assigned an activity score of zero. Any compound with a percent inhibition value >50% at any test concentration was assigned an activity score greater than zero. Activity score was then ranked by the potency, with the most potent compounds assigned the highest activity scores.

List of Reagents:

Dulbecco's Modified Eagle's Media I (Invitrogen, part 11965-092)
Dulbecco's Modified Eagle's Media, no Phenol Red (Invitrogen, part 21063-029)
Fetal Bovine Serum (Hyclone, part SH30088-03)
100X Penicillin-Streptomycin-Neomycin mix (Invitrogen, part 15640-055)
Recombinant human IL-6 (Peprotech, part 200-06)
Nifuroxazide (Sigma-Aldrich, part N 2641)
SteadyLite HTS Assay Kit (PerkinElmer, part 6016989)
T175 Flasks (Corning, part 431080)
1536-well plates (Greiner, part 789173)
Comment
Due to the increasing size of the MLPCN compound library, this assay may have been run as two or more separate campaigns, each campaign testing a unique set of compounds. All data were normalized on a per-plate basis. In this assay the inhibitor nifuroxazide had an IC50 in the range of 5-13 micromolar. Possible artifacts of this assay can include, but are not limited to: dust or lint located in or on wells of the plate, compounds that non-specifically modulate STAT3 or luciferase activity, and compounds that quench or enhance luminescence within the well. All test compound concentrations reported are nominal; the specific test concentration for a particular compound may vary based upon the actual sample provided by the MLSMR. The MLSMR was not able to provide all compounds selected for testing in this AID.
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1QualifierActivity Qualifier identifies if the resultant data IC50 came from a fitted curve or was determined manually to be less than or greater than its listed IC50 concentration.String
2IC50*The concentration at which 50 percent of the activity in the inhibitor assay is observed; (IC50) shown in micromolar.FloatμM
3LogIC50Log10 of the qualified IC50 (IC50) from the inhibitor assay in M concentrationFloat
4Hill SlopeThe variable HillSlope describes the steepness of the curve. This variable is called the Hill slope, the slope factor, or the Hill coefficient. If it is positive, the curve increases as X increases. If it is negative, the curve decreases as X increases. A standard sigmoid dose-response curve (previous equation) has a Hill Slope of 1.0. When HillSlope is less than 1.0, the curve is more shallow. When HillSlope is greater than 1.0, the curve is steeper. The Hill slope has no units.Float
5Hill S0Y-min of the curve.Float
6Hill SinfY-max of the curveFloat
7Hill dSThe range of Y.Float
8Chi SquareA measure for the 'goodness' of a fit. The chi-square test (Snedecor and Cochran, 1989) is used to test if a sample of data came from a population with a specific distribution.Float
9RsquareThis statistic measures how successful the fit explains the variation of the data; R-square is the square of the correlation between the response values and the predicted response values.Float
10Number of DataPointsOverall number of data points of normalized percent inhibition that was used for calculations (includes all concentration points); in some cases a data point can be excluded as outlier.Integer
11Inhibition at 3.0 nM (0.003μM**)Value of %inhibition at 3.0 nanomolar inhibitor concentration; average of triplicate measurement.Float%
12Inhibition at 8.0 nM (0.008μM**)Value of %inhibition at 8.0 nanomolar inhibitor concentration; average of triplicate measurement.Float%
13Inhibition at 30.0 nM (0.03μM**)Value of %inhibition at 30.0 nanomolar inhibitor concentration; average of triplicate measurement. Float%
14Inhibition at 80.0 nM (0.08μM**)Value of %inhibition at 80.0 nanomolar inhibitor concentration; average of triplicate measurement.Float%
15Inhibition at 200.0 nM (0.2μM**)Value of %inhibition at 200.0 nanomolar inhibitor concentration; average of triplicate measurement.Float%
16Inhibition at 0.7 uM (0.7μM**)Value of %inhibition at 0.7 micromolar inhibitor concentration; average of triplicate measurement.Float%
17Inhibition at 2.0 uM (2μM**)Value of %inhibition at 2.0 micromolar inhibitor concentration; average of triplicate measurement.Float%
18Inhibition at 6.0 uM (6μM**)Value of %inhibition at 6.0 micromolar inhibitor concentration; average of triplicate measurement.Float%
19Inhibition at 20.0 uM (20μM**)Value of %inhibition at 20.0 micromolar inhibitor concentration; average of triplicate measurement.Float%
20Inhibition at 60.0 uM (60μM**)Value of %inhibition at 60.0 micromolar inhibitor concentration; average of triplicate measurement.Float%

* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: 1 X01 MH079826-01

Data Table (Concise)
Classification
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