Bookmark and Share
BioAssay: AID 1056

SAR analysis of an In Vitro TNAP Dose Response Luminescent Assay

This TNAP dose response assay is developed and performed for the purpose of SAR study on analogs of hits originally identified in the TNAP luminescent HTS assay (AID 518) ..more
_
   
 Tested Compounds
 Tested Compounds
All(468)
 
 
Active(215)
 
 
Inactive(255)
 
 
 Tested Substances
 Tested Substances
All(485)
 
 
Active(226)
 
 
Inactive(259)
 
 
AID: 1056
Data Source: Burnham Center for Chemical Genomics (SDCCG-A048-TNAP (In Vitro SAR-1))
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Depositor Category: NIH Molecular Libraries Screening Center Network
BioAssay Version:
Deposit Date: 2008-02-28
Modify Date: 2010-11-12

Data Table ( Complete ):           View Active Data    View All Data
Target
BioActive Compounds: 215
Related Experiments
Show more
AIDNameTypeProbeComment
518TNAP luminescent HTS assayConfirmatory depositor-specified cross reference
1548Summary assay for compounds activating TNAP performed in a luminescent assaySummary2 depositor-specified cross reference
1574Summary assay for the identification of compounds that inhibit PHOSPHO1Summary1 depositor-specified cross reference
1577Summary luminescent assay for discovery of chemical inhibitors of placental alkaline phosphataseSummary2 depositor-specified cross reference
1659SAR assay for compounds activating TNAP in the presence of 100 mM DEA performed in a luminescence assayConfirmatory depositor-specified cross reference
813HTS identification of compounds activating TNAP at intermediate concentration of phosphate acceptor detected in luminescent assayScreening same project related to Summary assay
1001uHTS identification of compounds activating TNAP in the absence of phosphate acceptor performed in luminescent assayConfirmatory same project related to Summary assay
1136uHTS identification of compounds activating TNAP at a high concentration of phosphate acceptor detected in a luminescent assayScreening same project related to Summary assay
1227GAPDH Dose Response Colorimetric AssayConfirmatory same project related to Summary assay
1450SAR assay for compounds activating TNAP in the absence of phosphate acceptor performed in a luminescent assayConfirmatory same project related to Summary assay
1941SAR assay for compounds inhibiting TNAP in the absence of phosphate acceptor performed in a luminescent assayConfirmatory same project related to Summary assay
1535Confirmation of compounds inhibiting phosphomannose isomerase (PMI) via a fluorescence intensity assay.Confirmatory same project related to Summary assay
1565uHTS absorbance assay for the identification of compounds that inhibit PHOSPHO1Confirmatory same project related to Summary assay
1655Counter screen SAR assay for PMM2 inhibitors via a fluorescence intensity assayConfirmatory same project related to Summary assay
1666SAR assay for compounds that inhibit PHOSPHO1Confirmatory same project related to Summary assay
690Luminescent assay for HTS discovery of chemical inhibitors of placental alkaline phosphataseConfirmatory same project related to Summary assay
696Luminescent assay for HTS discovery of chemical activators of placental alkaline phosphataseConfirmatory same project related to Summary assay
1017Luminescent assay for identification of inhibitors of human intestinal alkaline phosphataseConfirmatory same project related to Summary assay
1019Luminescent assay for identification of inhibitors of bovine intestinal alkaline phosphataseScreening same project related to Summary assay
1512Luminescent assay for HTS discovery of chemical inhibitors of placental alkaline phosphatase confirmationConfirmatory same project related to Summary assay
Description:
Sanford-Burnham Center for Chemical Genomics (SBCCG)
Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA)
NIH Molecular Libraries Screening Centers Network (MLSCN)
Grant Number: MH077602-01
Assay Provider: Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA


This TNAP dose response assay is developed and performed for the purpose of SAR study on analogs of hits originally identified in the TNAP luminescent HTS assay (AID 518)

Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in the most organism. In human, four isozymes of APs have been identified. Three isozymes are tissue-specific and the fourth one is tissue-nonsepecifc, named TNAP. TNAP deficiency is associated with defective bone mineralization in the form of rickets and osteomalacia. Therefore, there are therapeutic potentials of inhibiting TNAP activity. The goal of this HTS is to identify novel and specific inhibitors of TNAP.
Protocol
TNAP assay materials:
1) TNAP protein was provided by Dr. Jose Luis Millan (Sanford-Burnham Medical Research Institute, San Diego, CA). The CDP-star was obtained from Applied Biosystems.
2) Assay Buffer: 250 mM DEA, pH 9.8, 2.5 mM MgCl2, and 0.05 mM ZnCl.
3) TNAP working solution contained a 1/800 dilution in assay buffer. Solution was prepared fresh prior to use.
4) CDP-star working solution contained 125 uM CDP-star in MQ water.
5) Levamisole working solution - 5 mM in 10% DMSO.

TNAP dose-response protocol:
1) Dose-response curves contained 10 concentrations of compounds obtained using 2-fold serial dilution. Compounds were serially diluted in 100% DMSO, and then diluted with water to 10% final DMSO concentration. 4 uL compounds in 10% DMSO were transferred into columns 3-22 of Greiner 384-well white small-volume plates (784075). Columns 1-2 and 23-24 contained 4 uL of levamisole working solution and 10% DMSO, respectively.
2) 8 uL of TNAP working solution was added to the whole plate using WellMate bulk dispenser (Matrix).
3) 8 uL of CDP-star working solution was added to the whole plate using WellMate bulk dispenser (Matrix).
4) Plates were incubated for 30 mins at room temperature.
5) Luminescence was measured on the Envision plate reader (Perkin Elmer).
6) Data analysis was performed using CBIS software (ChemInnovations, Inc) using sigmoidal dose-response equation through non-linear regression
Comment
The compounds were tested in 1 or more concentration ranges.
Range1 0-100 uM
Range2 0-1 uM
Range3 0-10 uM
Range4 0-20 uM
Range5 0-5 uM
Range6 0-0.5 uM
For replicates where the number of compounds tested were very low the data points have been omitted to reduce the number of columns in the results table.
The IC50 results for all curves were averaged to produce an IC50_Mean for each substance. Compounds with an IC50_Mean < 100 uM are considered to be active in this assay.
To simplify the distinction between the inactives of the primary screen and of the confirmatory screening stage, the Tiered Activity Scoring System was developed and implemented.
Activity Scoring
Activity scoring rules were devised to take into consideration compound efficacy, its potential interference with the assay and the screening stage that the data was obtained. Details of the Scoring System will be published elsewhere. Briefly, the outline of the scoring system utilized for the TNAP assay is as follows:
1) First tier (0-40 range) is reserved for primary screening data data and is not applicable to this assay.
2) Second tier (41-80 range) is reserved for dose-response confirmation data and is not applicable to this assay.
3) Third tier (81-100 range) is reserved for resynthesized true positives and their analogues
a. Inactive compounds of the confirmatory stage are assigned a score value equal 81.
b. The score is linearly correlated with a compound's inhibitory potency and, in addition, provides a measure of the likelihood that the compound is not an artifact based on the available information.
c. The Hill coefficient is taken as a measure of compound behavior in the assay via an additional scaling factor QC:
QC = 2.6*[exp(-0.5*nH^2) - exp(-1.5*nH^2)]
This empirical factor prorates the likelihood of target-specific compound effect vs. its non-specific behavior in the assay. This factor is based on expectation that a compound with a single mode of action that achieved equilibrium in the TNAP inhibition assay demonstrates the Hill coefficient value of 1. Compounds deviating from that behavior are penalized proportionally to the degree of their deviation.
d. Summary equation that takes into account the items discussed above is
Score = 82 + 3*(pIC50 - 3)*QC,
where pIC50 is a negative log(10) of the IC50 value expressed in mole/L concentration units. This equation results in the Score values above 50 for compounds that demonstrate high potency and predictable behavior. Compounds that are inactive in the assay or whose concentration-dependent behavior are likely to be an artifact of that assay will generally have lower Score values.
Categorized Comment - additional comments and annotations
From PubChem:
Assay Test Type: In vitro
From ChEMBL:
Assay Type: Binding
Result Definitions
Show more
TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1IC50_Mean_QualifierThis qualifier is to be used with the next TID, IC50_Mean. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
2IC50_Mean*IC50 value determined using sigmoidal dose response equationFloatμM
3IC50_Qualifier_1_Range1This qualifier is to be used with the next TID, IC50_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
4IC50_1_Range1IC50 value determined using sigmoidal dose response equationFloatμM
5Std.Err(IC50)_1_Range1Standard Error of IC50 valueFloatμM
6nH_1_Range1Hill coefficient determined using sigmoidal dose response equationFloat
7Excluded_Points_first_point_Range1Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
8% inhibition at 0.1953125 uM_first_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
9% inhibition at 0.390625 uM_first_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
10% inhibition at 0.78125 uM_first_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
11% inhibition at 1.5625 uM_first_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
12% inhibition at 3.125 uM_first_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
13% inhibition at 6.25 uM_first_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
14% inhibition at 12.5 uM_first_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
15% inhibition at 25 uM_first_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
16% inhibition at 50 uM_first_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
17% inhibition at 100 uM_first_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
18Excluded_Points_second_point_Range1Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
19% inhibition at 0.1953125 uM_second_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
20% inhibition at 0.390625 uM_second_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
21% inhibition at 0.78125 uM_second_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
22% inhibition at 1.5625 uM_second_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
23% inhibition at 3.125 uM_second_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
24% inhibition at 6.25 uM_second_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
25% inhibition at 12.5 uM_second_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
26% inhibition at 25 uM_second_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
27% inhibition at 50 uM_second_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
28% inhibition at 100 uM_second_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
29Excluded_Points_third_point_Range1Flags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
30IC50_Qualifier_2_Range1This qualifier is to be used with the next TID, IC50_2_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
31IC50_2_Range1IC50 value determined using sigmoidal dose response equationFloatμM
32Std.Err(IC50)_2_Range1Standard Error of IC50 valueFloatμM
33nH_2_Range1Hill coefficient determined using sigmoidal dose response equationFloat
34% inhibition at 100 uM_third_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
35% inhibition at 50 uM_third_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
36% inhibition at 25 uM_third_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
37% inhibition at 12.5 uM_third_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
38% inhibition at 6.25 uM_third_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
39% inhibition at 3.125 uM_third_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
40% inhibition at 1.5625 uM_third_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
41% inhibition at 0.78125 uM_third_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
42% inhibition at 0.390625 uM_third_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
43% inhibition at 0.1953125 uM_third_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
44Excluded_Points_fourth_point_Range1Flags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
45% inhibition at 100 uM_fourth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
46% inhibition at 50 uM_fourth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
47% inhibition at 25 uM_fourth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
48% inhibition at 12.5 uM_fourth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
49% inhibition at 6.25 uM_fourth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
50% inhibition at 3.125 uM_fourth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
51% inhibition at 1.5625 uM_fourth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
52% inhibition at 0.78125 uM_fourth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
53% inhibition at 0.390625 uM_fourth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
54% inhibition at 0.1953125 uM_fourth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
55IC50_Qualifier_3_Range1This qualifier is to be used with the next TID, IC50_3_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
56IC50_3_Range1IC50 value determined using sigmoidal dose response equationFloatμM
57Std.Err(IC50)_3_Range1Standard Error of IC50 valueFloatμM
58nH_3_Range1Hill coefficient determined using sigmoidal dose response equationFloat
59Excluded_Points_fifth_point_Range1Flags to indicate which of the fifth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
60% inhibition at 100 uM_fifth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
61% inhibition at 50 uM_fifth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
62% inhibition at 25 uM_fifth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
63% inhibition at 12.5 uM_fifth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
64% inhibition at 6.25 uM_fifth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
65% inhibition at 3.125 uM_fifth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
66% inhibition at 1.5625 uM_fifth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
67% inhibition at 0.78125 uM_fifth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
68% inhibition at 0.390625 uM_fifth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
69% inhibition at 0.1953125 uM_fifth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
70Excluded_Points_sixth_point_Range1Flags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
71% inhibition at 100 uM_sixth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
72% inhibition at 50 uM_sixth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
73% inhibition at 25 uM_sixth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
74% inhibition at 12.5 uM_sixth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
75% inhibition at 6.25 uM_sixth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
76% inhibition at 3.125 uM_sixth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
77% inhibition at 1.5625 uM_sixth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
78% inhibition at 0.78125 uM_sixth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
79% inhibition at 0.390625 uM_sixth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
80% inhibition at 0.1953125 uM_sixth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
81IC50_Qualifier_4_Range1This qualifier is to be used with the next TID, IC50_4_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
82IC50_4_Range1IC50 value determined using sigmoidal dose response equationFloatμM
83Std.Err(IC50)_4_Range1Standard Error of IC50 valueFloatμM
84nH_4_Range1Hill coefficient determined using sigmoidal dose response equationFloat
85Excluded_Points_seventh_point_Range1Flags to indicate which of the seventh dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
86% inhibition at 100 uM_seventh_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
87% inhibition at 50 uM_seventh_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
88% inhibition at 25 uM_seventh_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
89% inhibition at 12.5 uM_seventh_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
90% inhibition at 6.25 uM_seventh_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
91% inhibition at 3.125 uM_seventh_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
92% inhibition at 1.5625 uM_seventh_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
93% inhibition at 0.78125 uM_seventh_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
94% inhibition at 0.390625 uM_seventh_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
95% inhibition at 0.1953125 uM_seventh_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
96Excluded_Points_eighth_point_Range1Flags to indicate which of the eigth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
97% inhibition at 100 uM_eighth_point_Range1 (100μM**)% inhibition at a given concentrationFloat%
98% inhibition at 50 uM_eighth_point_Range1 (50μM**)% inhibition at a given concentrationFloat%
99% inhibition at 25 uM_eighth_point_Range1 (25μM**)% inhibition at a given concentrationFloat%
100% inhibition at 12.5 uM_eighth_point_Range1 (12.5μM**)% inhibition at a given concentrationFloat%
101% inhibition at 6.25 uM_eighth_point_Range1 (6.25μM**)% inhibition at a given concentrationFloat%
102% inhibition at 3.125 uM_eighth_point_Range1 (3.125μM**)% inhibition at a given concentrationFloat%
103% inhibition at 1.5625 uM_eighth_point_Range1 (1.5625μM**)% inhibition at a given concentrationFloat%
104% inhibition at 0.78125 uM_eighth_point_Range1 (0.78125μM**)% inhibition at a given concentrationFloat%
105% inhibition at 0.390625 uM_eighth_point_Range1 (0.390625μM**)% inhibition at a given concentrationFloat%
106% inhibition at 0.1953125 uM_eighth_point_Range1 (0.195312μM**)% inhibition at a given concentrationFloat%
107IC50_Qualifier_5_Range1This qualifier is to be used with the next TID, IC50_5_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
108IC50_5_Range1IC50 value determined using sigmoidal dose response equationFloatμM
109Std.Err(IC50)_5_Range1Standard Error of IC50 valueFloatμM
110nH_5_Range1Hill coefficient determined using sigmoidal dose response equationFloat
111IC50_Qualifier_6_Range1This qualifier is to be used with the next TID, IC50_6_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
112IC50_6_Range1IC50 value determined using sigmoidal dose response equationFloatμM
113Std.Err(IC50)_6_Range1Standard Error of IC50 valueFloatμM
114nH_6_Range1Hill coefficient determined using sigmoidal dose response equationFloat
115IC50_Qualifier_7_Range1This qualifier is to be used with the next TID, IC50_7_Range1. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
116IC50_7_Range1IC50 value determined using sigmoidal dose response equationFloatμM
117Std.Err(IC50)_7_Range1Standard Error of IC50 valueFloatμM
118nH_7_Range1Hill coefficient determined using sigmoidal dose response equationFloat
119IC50_Qualifier_1_Range2This qualifier is to be used with the next TID, IC50_1_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
120IC50_1_Range2IC50 value determined using sigmoidal dose response equationFloatμM
121Std.Err(IC50)_1_Range2Standard Error of IC50 valueFloatμM
122nH_1_Range2Hill coefficient determined using sigmoidal dose response equationFloat
123Excluded_Points_first_point_Range2Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
124% inhibition at 1 uM_first_point_Range2 (0.0195312μM**)% inhibition at a given concentrationFloat%
125% inhibition at 0.5 uM_first_point_Range2 (10μM**)% inhibition at a given concentrationFloat%
126% inhibition at 0.25 uM_first_point_Range2 (5μM**)% inhibition at a given concentrationFloat%
127% inhibition at 0.125 uM_first_point_Range2 (2.5μM**)% inhibition at a given concentrationFloat%
128% inhibition at 0.0625 uM_first_point_Range2 (1.25μM**)% inhibition at a given concentrationFloat%
129% inhibition at 0.03125 uM_first_point_Range2 (0.625μM**)% inhibition at a given concentrationFloat%
130% inhibition at 0.015625 uM_first_point_Range2 (0.3125μM**)% inhibition at a given concentrationFloat%
131% inhibition at 0.0078125 uM_first_point_Range2 (0.15625μM**)% inhibition at a given concentrationFloat%
132% inhibition at 0.00390625 uM_first_point_Range2 (0.078125μM**)% inhibition at a given concentrationFloat%
133% inhibition at 0.001953125 uM_first_point_Range2 (0.0390625μM**)% inhibition at a given concentrationFloat%
134Excluded_Points_second_point_Range2Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
135% inhibition at 1 uM_second_point_Range2 (10μM**)% inhibition at a given concentrationFloat%
136% inhibition at 0.5 uM_second_point_Range2 (5μM**)% inhibition at a given concentrationFloat%
137% inhibition at 0.25 uM_second_point_Range2 (2.5μM**)% inhibition at a given concentrationFloat%
138% inhibition at 0.125 uM_second_point_Range2 (1.25μM**)% inhibition at a given concentrationFloat%
139% inhibition at 0.0625 uM_second_point_Range2 (0.625μM**)% inhibition at a given concentrationFloat%
140% inhibition at 0.03125 uM_second_point_Range2 (0.3125μM**)% inhibition at a given concentrationFloat%
141% inhibition at 0.015625 uM_second_point_Range2 (0.15625μM**)% inhibition at a given concentrationFloat%
142% inhibition at 0.0078125 uM_second_point_Range2 (0.0390625μM**)% inhibition at a given concentrationFloat%
143% inhibition at 0.00390625 uM_second_point_Range2 (0.0195312μM**)% inhibition at a given concentrationFloat%
144% inhibition at 0.001953125 uM_second_point_Range2 (0.078125μM**)% inhibition at a given concentrationFloat%
145IC50_Qualifier_2_Range2This qualifier is to be used with the next TID, IC50_2_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
146IC50_2_Range2IC50 value determined using sigmoidal dose response equationFloatμM
147Std.Err(IC50)_2_Range2Standard Error of IC50 valueFloatμM
148nH_2_Range2Hill coefficient determined using sigmoidal dose response equationFloat
149Excluded_Points_third_point_Range2Flags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
150% inhibition at 1 uM_third_point_Range2 (1μM**)% inhibition at a given concentrationFloat%
151% inhibition at 0.5 uM_third_point_Range2 (0.5μM**)% inhibition at a given concentrationFloat%
152% inhibition at 0.25 uM_third_point_Range2 (0.25μM**)% inhibition at a given concentrationFloat%
153% inhibition at 0.125 uM_third_point_Range2 (0.125μM**)% inhibition at a given concentrationFloat%
154% inhibition at 0.0625 uM_third_point_Range2 (0.0625μM**)% inhibition at a given concentrationFloat%
155% inhibition at 0.03125 uM_third_point_Range2 (0.03125μM**)% inhibition at a given concentrationFloat%
156% inhibition at 0.015625 uM_third_point_Range2 (0.015625μM**)% inhibition at a given concentrationFloat%
157% inhibition at 0.0078125 uM_third_point_Range2 (0.0078125μM**)% inhibition at a given concentrationFloat%
158% inhibition at 0.00390625 uM_third_point_Range2 (0.00390625μM**)% inhibition at a given concentrationFloat%
159% inhibition at 0.001953125 uM_third_point_Range2 (0.00195312μM**)% inhibition at a given concentrationFloat%
160Excluded_Points_fourth_point_Range2Flags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
161% inhibition at 1 uM_fourth_point_Range2 (1μM**)% inhibition at a given concentrationFloat%
162% inhibition at 0.5 uM_fourth_point_Range2 (0.5μM**)% inhibition at a given concentrationFloat%
163% inhibition at 0.25 uM_fourth_point_Range2 (0.25μM**)% inhibition at a given concentrationFloat%
164% inhibition at 0.125 uM_fourth_point_Range2 (0.125μM**)% inhibition at a given concentrationFloat%
165% inhibition at 0.0625 uM_fourth_point_Range2 (0.0625μM**)% inhibition at a given concentrationFloat%
166% inhibition at 0.03125 uM_fourth_point_Range2 (0.03125μM**)% inhibition at a given concentrationFloat%
167% inhibition at 0.015625 uM_fourth_point_Range2 (0.015625μM**)% inhibition at a given concentrationFloat%
168% inhibition at 0.0078125 uM_fourth_point_Range2 (0.0078125μM**)% inhibition at a given concentrationFloat%
169% inhibition at 0.00390625 uM_fourth_point_Range2 (0.00390625μM**)% inhibition at a given concentrationFloat%
170% inhibition at 0.001953125 uM_fourth_point_Range2 (0.00195312μM**)% inhibition at a given concentrationFloat%
171IC50_Qualifier_3_Range2This qualifier is to be used with the next TID, IC50_3_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
172IC50_3_Range2IC50 value determined using sigmoidal dose response equationFloatμM
173Std.Err(IC50)_3_Range2Standard Error of IC50 valueFloatμM
174nH_3_Range2Hill coefficient determined using sigmoidal dose response equationFloat
175IC50_Qualifier_4_Range2This qualifier is to be used with the next TID, IC50_4_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
176IC50_4_Range2IC50 value determined using sigmoidal dose response equationFloatμM
177Std.Err(IC50)_4_Range2Standard Error of IC50 valueFloatμM
178nH_4_Range2Hill coefficient determined using sigmoidal dose response equationFloat
179IC50_Qualifier_5_Range2This qualifier is to be used with the next TID, IC50_5_Range2 If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
180IC50_5_Range2IC50 value determined using sigmoidal dose response equationFloatμM
181Std.Err(IC50)_5_Range2Standard Error of IC50 valueFloatμM
182nH_5_Range2Hill coefficient determined using sigmoidal dose response equationFloat
183IC50_Qualifier_6_Range2This qualifier is to be used with the next TID, IC50_6_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
184IC50_6_Range2IC50 value determined using sigmoidal dose response equationFloatμM
185Std.Err(IC50)_6_Range2Standard Error of IC50 valueFloatμM
186nH_6_Range2Hill coefficient determined using sigmoidal dose response equationFloat
187IC50_Qualifier_7_Range2This qualifier is to be used with the next TID, IC50_7_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
188IC50_7_Range2IC50 value determined using sigmoidal dose response equationFloatμM
189Std.Err(IC50)_7_Range2Standard Error of IC50 valueFloatμM
190nH_7_Range2Hill coefficient determined using sigmoidal dose response equationFloat
191IC50_Qualifier_8_Range2This qualifier is to be used with the next TID, IC50_8_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
192IC50_8_Range2IC50 value determined using sigmoidal dose response equationFloatμM
193Std.Err(IC50)_8_Range2Standard Error of IC50 valueFloatμM
194nH_8_Range2Hill coefficient determined using sigmoidal dose response equationFloat
195IC50_Qualifier_9_Range2This qualifier is to be used with the next TID, IC50_9_Range2. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
196IC50_9_Range2IC50 value determined using sigmoidal dose response equationFloatμM
197Std.Err(IC50)_9_Range2Standard Error of IC50 valueFloatμM
198nH_9_Range2Hill coefficient determined using sigmoidal dose response equationFloat
199IC50_Qualifier_1_Range3This qualifier is to be used with the next TID, IC50_1_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
200IC50_1_Range3IC50 value determined using sigmoidal dose response equationFloatμM
201Std.Err(IC50)_1_Range3Standard Error of IC50 valueFloatμM
202nH_1_Range3Hill coefficient determined using sigmoidal dose response equationFloat
203Excluded_Points_first_point_Range3Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
204% inhibition at 10 uM_first_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
205% inhibition at 5 uM_first_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
206% inhibition at 2.5 uM_first_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
207% inhibition at 1.25 uM_first_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
208% inhibition at 0.625 uM_first_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
209% inhibition at 0.3125 uM_first_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
210% inhibition at 0.15625 uM_first_point_Range3 (0.00976562μM**)% inhibition at a given concentrationFloat%
211% inhibition at 0.078125 uM_first_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
212% inhibition at 0.0390625 uM_first_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
213% inhibition at 0.01953125 uM_first_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
214Excluded_Points_second_point_Range3Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
215% inhibition at 10 uM_second_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
216% inhibition at 5 uM_second_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
217% inhibition at 2.5 uM_second_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
218% inhibition at 1.25 uM_second_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
219% inhibition at 0.625 uM_second_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
220% inhibition at 0.3125 uM_second_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
221% inhibition at 0.15625 uM_second_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
222% inhibition at 0.078125 uM_second_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
223% inhibition at 0.0390625 uM_second_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
224% inhibition at 0.01953125 uM_second_point_Range3 (0.00976562μM**)% inhibition at a given concentrationFloat%
225IC50_Qualifier_2_Range3This qualifier is to be used with the next TID, IC50_2_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
226IC50_2_Range3IC50 value determined using sigmoidal dose response equationFloat
227Std.Err(IC50)_2_Range3Standard Error of IC50 valueFloatμM
228nH_2_Range3Hill coefficient determined using sigmoidal dose response equationFloatμM
229Excluded_Points_third_point_Range3Flags to indicate which of the third dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
230% inhibition at 10 uM_third_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
231% inhibition at 5 uM_third_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
232% inhibition at 2.5 uM_third_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
233% inhibition at 1.25 uM_third_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
234% inhibition at 0.625 uM_third_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
235% inhibition at 0.3125 uM_third_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
236% inhibition at 0.15625 uM_third_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
237% inhibition at 0.078125 uM_third_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
238% inhibition at 0.0390625 uM_third_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
239% inhibition at 0.01953125 uM_third_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
240Excluded_Points_fourth_point_Range3Flags to indicate which of the fourth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
241% inhibition at 10 uM_fourth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
242% inhibition at 5 uM_fourth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
243% inhibition at 2.5 uM_fourth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
244% inhibition at 1.25 uM_fourth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
245% inhibition at 0.625 uM_fourth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
246% inhibition at 0.3125 uM_fourth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
247% inhibition at 0.15625 uM_fourth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
248% inhibition at 0.078125 uM_fourth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
249% inhibition at 0.0390625 uM_fourth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
250% inhibition at 0.01953125 uM_fourth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
251IC50_Qualifier_3_Range3This qualifier is to be used with the next TID, IC50_3_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
252IC50_3_Range3IC50 value determined using sigmoidal dose response equationFloatμM
253Std.Err(IC50)_3_Range3Standard Error of IC50 valueFloatμM
254nH_3_Range3Hill coefficient determined using sigmoidal dose response equationFloat
255Excluded_Points_fifth_point_Range3Flags to indicate which of the fifth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
256% inhibition at 10 uM_fifth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
257% inhibition at 5 uM_fifth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
258% inhibition at 2.5 uM_fifth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
259% inhibition at 1.25 uM_fifth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
260% inhibition at 0.625 uM_fifth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
261% inhibition at 0.3125 uM_fifth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
262% inhibition at 0.15625 uM_fifth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
263% inhibition at 0.078125 uM_fifth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
264% inhibition at 0.0390625 uM_fifth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
265% inhibition at 0.01953125 uM_fifth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
266Excluded_Points_sixth_point_Range3Flags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
267% inhibition at 10 uM_sixth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
268% inhibition at 5 uM_sixth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
269% inhibition at 2.5 uM_sixth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
270% inhibition at 1.25 uM_sixth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
271% inhibition at 0.625 uM_sixth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
272% inhibition at 0.3125 uM_sixth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
273% inhibition at 0.15625 uM_sixth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
274% inhibition at 0.078125 uM_sixth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
275% inhibition at 0.0390625 uM_sixth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
276% inhibition at 0.01953125 uM_sixth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
277IC50_Qualifier_4_Range3This qualifier is to be used with the next TID, IC50_4_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
278IC50_4_Range3IC50 value determined using sigmoidal dose response equationFloatμM
279Std.Err(IC50)_4_Range3Standard Error of IC50 valueFloatμM
280nH_4_Range3Hill coefficient determined using sigmoidal dose response equationFloat
281Excluded_Points_seventh_point_Range3Flags to indicate which of the seventh dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
282% inhibition at 10 uM_seventh_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
283% inhibition at 5 uM_seventh_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
284% inhibition at 2.5 uM_seventh_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
285% inhibition at 1.25 uM_seventh_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
286% inhibition at 0.625 uM_seventh_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
287% inhibition at 0.3125 uM_seventh_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
288% inhibition at 0.15625 uM_seventh_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
289% inhibition at 0.078125 uM_seventh_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
290% inhibition at 0.0390625 uM_seventh_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
291% inhibition at 0.01953125 uM_seventh_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
292Excluded_Points_eight_point_Range3Flags to indicate which of the eighth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
293% inhibition at 10 uM_eight_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
294% inhibition at 5 uM_eight_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
295% inhibition at 2.5 uM_eight_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
296% inhibition at 1.25 uM_eight_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
297% inhibition at 0.625 uM_eight_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
298% inhibition at 0.3125 uM_eight_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
299% inhibition at 0.15625 uM_eight_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
300% inhibition at 0.078125 uM_eight_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
301% inhibition at 0.0390625 uM_eight_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
302% inhibition at 0.01953125 uM_eight_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
303IC50_Qualifier_5_Range3This qualifier is to be used with the next TID, IC50_5_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
304IC50_5_Range3IC50 value determined using sigmoidal dose response equationFloatμM
305Std.Err(IC50)_5_Range3Standard Error of IC50 valueFloatμM
306nH_5_Range3Hill coefficient determined using sigmoidal dose response equationFloat
307Excluded_Points_ninth_point_Range3Flags to indicate which of the ninth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
308% inhibition at 10 uM_ninth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
309% inhibition at 5 uM_ninth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
310% inhibition at 2.5 uM_ninth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
311% inhibition at 1.25 uM_ninth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
312% inhibition at 0.625 uM_ninth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
313% inhibition at 0.3125 uM_ninth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
314% inhibition at 0.15625 uM_ninth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
315% inhibition at 0.078125 uM_ninth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
316% inhibition at 0.0390625 uM_ninth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
317% inhibition at 0.01953125 uM_ninth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
318Excluded_Points_tenth_point_Range3Flags to indicate which of the tenth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
319% inhibition at 10 uM_tenth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
320% inhibition at 5 uM_tenth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
321% inhibition at 2.5 uM_tenth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
322% inhibition at 1.25 uM_tenth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
323% inhibition at 0.625 uM_tenth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
324% inhibition at 0.3125 uM_tenth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
325% inhibition at 0.15625 uM_tenth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
326% inhibition at 0.078125 uM_tenth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
327% inhibition at 0.0390625 uM_tenth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
328% inhibition at 0.01953125 uM_tenth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
329IC50_Qualifier_6_Range3This qualifier is to be used with the next TID, IC50_6_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
330IC50_6_Range3IC50 value determined using sigmoidal dose response equationFloatμM
331Std.Err(IC50)_6_Range3Standard Error of IC50 valueFloatμM
332nH_6_Range3Hill coefficient determined using sigmoidal dose response equationFloat
333Excluded_Points_eleventh_point_Range3Flags to indicate which of the eleventh dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
334% inhibition at 10 uM_eleventh_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
335% inhibition at 5 uM_eleventh_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
336% inhibition at 2.5 uM_eleventh_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
337% inhibition at 1.25 uM_eleventh_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
338% inhibition at 0.625 uM_eleventh_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
339% inhibition at 0.3125 uM_eleventh_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
340% inhibition at 0.15625 uM_eleventh_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
341% inhibition at 0.078125 uM_eleventh_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
342% inhibition at 0.01953125 uM_eleventh_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
343% inhibition at 0.0390625 uM_eleventh_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
344Excluded_Points_twelfth_point_Range3Flags to indicate which of the twelfth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
345% inhibition at 10 uM_twelfth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
346% inhibition at 5 uM_twelfth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
347% inhibition at 2.5 uM_twelfth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
348% inhibition at 1.25 uM_twelfth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
349% inhibition at 0.625 uM_twelfth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
350% inhibition at 0.3125 uM_twelfth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
351% inhibition at 0.15625 uM_twelfth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
352% inhibition at 0.078125 uM_twelfth_point_Range3 (0.78125μM**)% inhibition at a given concentrationFloat%
353% inhibition at 0.0390625 uM_twelfth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
354% inhibition at 0.01953125 uM_twelfth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
355IC50_Qualifier_7_Range3This qualifier is to be used with the next TID, IC50__Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
356IC50_7_Range3IC50 value determined using sigmoidal dose response equationFloatμM
357Std.Err(IC50)_7_Range3Standard Error of IC50 valueFloatμM
358nH_7_Range3Hill coefficient determined using sigmoidal dose response equationFloat
359Excluded_Points_thirteenth_point_Range3Flags to indicate which of the thirteenth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
360% inhibition at 10 uM_thirteenth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
361% inhibition at 5 uM_thirteenth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
362% inhibition at 2.5 uM_thirteenth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
363% inhibition at 1.25 uM_thirteenth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
364% inhibition at 0.625 uM_thirteenth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
365% inhibition at 0.3125 uM_thirteenth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
366% inhibition at 0.15625 uM_thirteenth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
367% inhibition at 0.078125 uM_thirteenth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
368% inhibition at 0.0390625 uM_thirteenth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
369% inhibition at 0.01953125 uM_thirteenth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
370Excluded_Points_fourteenth_point_Range3Flags to indicate which of the sixth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
371% inhibition at 10 uM_fourteenth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
372% inhibition at 5 uM_fourteenth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
373% inhibition at 2.5 uM_fourteenth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
374% inhibition at 1.25 uM_fourteenth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
375% inhibition at 0.625 uM_fourteenth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
376% inhibition at 0.3125 uM_fourteenth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
377% inhibition at 0.15625 uM_fourteenth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
378% inhibition at 0.078125 uM_fourteenth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
379% inhibition at 0.0390625 uM_fourteenth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
380% inhibition at 0.01953125 uM_fourteenth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
381IC50_Qualifier_8_Range3This qualifier is to be used with the next TID, IC50_8_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
382IC50_8_Range3IC50 value determined using sigmoidal dose response equationFloatμM
383Std.Err(IC50)_8_Range3Standard Error of IC50 valueFloatμM
384nH_8_Range3Hill coefficient determined using sigmoidal dose response equationFloat
385Excluded_Points_fifteenth_point_Range3Flags to indicate which of the fifteenth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
386% inhibition at 10 uM_fifteenth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
387% inhibition at 5 uM_fifteenth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
388% inhibition at 2.5 uM_fifteenth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
389% inhibition at 1.25 uM_fifteenth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
390% inhibition at 0.625 uM_fifteenth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
391% inhibition at 0.3125 uM_fifteenth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
392% inhibition at 0.15625 uM_fifteenth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
393% inhibition at 0.078125 uM_fifteenth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
394% inhibition at 0.0390625 uM_fifteenth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
395% inhibition at 0.01953125 uM_fifteenth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
396Excluded_Points_sixteenth_point_Range3Flags to indicate which of the sixteenth dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
397% inhibition at 10 uM_sixteenth_point_Range3 (10μM**)% inhibition at a given concentrationFloat%
398% inhibition at 5 uM_sixteenth_point_Range3 (5μM**)% inhibition at a given concentrationFloat%
399% inhibition at 2.5 uM_sixteenth_point_Range3 (2.5μM**)% inhibition at a given concentrationFloat%
400% inhibition at 1.25 uM_sixteenth_point_Range3 (1.25μM**)% inhibition at a given concentrationFloat%
401% inhibition at 0.625 uM_sixteenth_point_Range3 (0.625μM**)% inhibition at a given concentrationFloat%
402% inhibition at 0.3125 uM_sixteenth_point_Range3 (0.3125μM**)% inhibition at a given concentrationFloat%
403% inhibition at 0.15625 uM_sixteenth_point_Range3 (0.15625μM**)% inhibition at a given concentrationFloat%
404% inhibition at 0.078125 uM_sixteenth_point_Range3 (0.078125μM**)% inhibition at a given concentrationFloat%
405% inhibition at 0.0390625 uM_sixteenth_point_Range3 (0.0390625μM**)% inhibition at a given concentrationFloat%
406% inhibition at 0.01953125 uM_sixteenth_point_Range3 (0.0195312μM**)% inhibition at a given concentrationFloat%
407IC50_Qualifier_9_Range3This qualifier is to be used with the next TID, IC50_9_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
408IC50_9_Range3IC50 value determined using sigmoidal dose response equationFloatμM
409Std.Err(IC50)_9_Range3Standard Error of IC50 valueFloatμM
410nH_9_Range3Hill coefficient determined using sigmoidal dose response equationFloat
411IC50_Qualifier_10_Range3This qualifier is to be used with the next TID, IC50_10_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
412IC50_10_Range3IC50 value determined using sigmoidal dose response equationFloatμM
413Std.Err(IC50)_10_Range3Standard Error of IC50 valueFloatμM
414nH_10_Range3Hill coefficient determined using sigmoidal dose response equationFloat
415IC50_Qualifier_11_Range3This qualifier is to be used with the next TID, IC50_11_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
416IC50_11_Range3IC50 value determined using sigmoidal dose response equationFloatμM
417Std.Err(IC50)_11_Range3Standard Error of IC50 valueFloatμM
418nH_11_Range3Hill coefficient determined using sigmoidal dose response equationFloat
419IC50_Qualifier_12_Range3This qualifier is to be used with the next TID, IC50_12_Range3. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
420IC50_12_Range3IC50 value determined using sigmoidal dose response equationFloatμM
421Std.Err(IC50)_12_Range3Standard Error of IC50 valueFloatμM
422nH_12_Range3Hill coefficient determined using sigmoidal dose response equationFloat
423IC50_Qualifier_1_Range4This qualifier is to be used with the next TID, IC50_1_Range4. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
424IC50_1_Range4IC50 value determined using sigmoidal dose response equationFloatμM
425Std.Err(IC50)_1_Range4Standard Error of IC50 valueFloatμM
426nH_1_Range4Hill coefficient determined using sigmoidal dose response equationFloat
427Excluded_Points_first_point_Range4Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
428% inhibition at 20 uM_first_point_Range4 (50μM**)% inhibition at a given concentrationFloat%
429% inhibition at 10 uM_first_point_Range4 (25μM**)% inhibition at a given concentrationFloat%
430% inhibition at 5 uM_first_point_Range4 (12.5μM**)% inhibition at a given concentrationFloat%
431% inhibition at 2.5 uM_first_point_Range4 (6.25μM**)% inhibition at a given concentrationFloat%
432% inhibition at 1.25 uM_first_point_Range4 (3.125μM**)% inhibition at a given concentrationFloat%
433% inhibition at 0.625 uM_first_point_Range4 (1.5625μM**)% inhibition at a given concentrationFloat%
434% inhibition at 0.3125 uM_first_point_Range4 (0.78125μM**)% inhibition at a given concentrationFloat%
435% inhibition at 0.15625 uM_first_point_Range4 (0.390625μM**)% inhibition at a given concentrationFloat%
436% inhibition at 0.078125 uM_first_point_Range4 (0.0195312μM**)% inhibition at a given concentrationFloat%
437% inhibition at 0.0390625 uM_first_point_Range4 (0.0976562μM**)% inhibition at a given concentrationFloat%
438Excluded_Points_second_point_Range4Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
439% inhibition at 20 uM_second_point_Range4 (50μM**)% inhibition at a given concentrationFloat%
440% inhibition at 10 uM_second_point_Range4 (25μM**)% inhibition at a given concentrationFloat%
441% inhibition at 5 uM_second_point_Range4 (12.5μM**)% inhibition at a given concentrationFloat%
442% inhibition at 2.5 uM_second_point_Range4 (6.25μM**)% inhibition at a given concentrationFloat%
443% inhibition at 1.25 uM_second_point_Range4 (3.125μM**)% inhibition at a given concentrationFloat%
444% inhibition at 0.625 uM_second_point_Range4 (1.5625μM**)% inhibition at a given concentrationFloat%
445% inhibition at 0.3125 uM_second_point_Range4 (0.78125μM**)% inhibition at a given concentrationFloat%
446% inhibition at 0.15625 uM_second_point_Range4 (0.390625μM**)% inhibition at a given concentrationFloat%
447% inhibition at 0.078125 uM_second_point_Range4 (0.195312μM**)% inhibition at a given concentrationFloat%
448% inhibition at 0.0390625 uM_second_point_Range4 (0.0976562μM**)% inhibition at a given concentrationFloat%
449IC50_Qualifier_1_Range5This qualifier is to be used with the next TID, IC50_1_Range5. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
450IC50_1_Range5IC50 value determined using sigmoidal dose response equationFloatμM
451Std.Err(IC50)_1_Range5Standard Error of IC50 valueFloatμM
452nH_1_Range5Hill coefficient determined using sigmoidal dose response equationFloat
453Excluded_Points_first_point_Range5Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
454% inhibition at 5 uM_first_point_Range5 (5μM**)% inhibition at a given concentrationFloat%
455% inhibition at 2.5 uM_first_point_Range5 (2.5μM**)% inhibition at a given concentrationFloat%
456% inhibition at 1.25 uM_first_point_Range5 (1.25μM**)% inhibition at a given concentrationFloat%
457% inhibition at 0.625 uM_first_point_Range5 (0.625μM**)% inhibition at a given concentrationFloat%
458% inhibition at 0.3125 uM_first_point_Range5 (0.3215μM**)% inhibition at a given concentrationFloat%
459% inhibition at 0.15625 uM_first_point_Range5 (0.15625μM**)% inhibition at a given concentrationFloat%
460% inhibition at 0.078125 uM_first_point_Range5 (0.078125μM**)% inhibition at a given concentrationFloat%
461% inhibition at 0.0390625 uM_first_point_Range5 (0.0390625μM**)% inhibition at a given concentrationFloat%
462% inhibition at 0.01953125 uM_first_point_Range5 (0.0195312μM**)% inhibition at a given concentrationFloat%
463% inhibition at 0.009765625 uM_first_point_Range5 (0.00976562μM**)% inhibition at a given concentrationFloat%
464Excluded_Points_second_point_Range5Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
465% inhibition at 5 uM_second_point_Range5 (5μM**)% inhibition at a given concentrationFloat%
466% inhibition at 2.5 uM_second_point_Range5 (2.5μM**)% inhibition at a given concentrationFloat%
467% inhibition at 1.25 uM_second_point_Range5 (1.25μM**)% inhibition at a given concentrationFloat%
468% inhibition at 0.625 uM_second_point_Range5 (0.625μM**)% inhibition at a given concentrationFloat%
469% inhibition at 0.3125 uM_second_point_Range5 (0.3125μM**)% inhibition at a given concentrationFloat%
470% inhibition at 0.15625 uM_second_point_Range5 (0.15625μM**)% inhibition at a given concentrationFloat%
471% inhibition at 0.078125 uM_second_point_Range5 (0.078125μM**)% inhibition at a given concentrationFloat%
472% inhibition at 0.0390625 uM_second_point_Range5 (0.0390625μM**)% inhibition at a given concentrationFloat%
473% inhibition at 0.01953125 uM_second_point_Range5 (0.0195312μM**)% inhibition at a given concentrationFloat%
474% inhibition at 0.009765625 uM_second_point_Range5 (0.00976562μM**)% inhibition at a given concentrationFloat%
475IC50_Qualifier_1_Range6This qualifier is to be used with the next TID, IC50_1_Range6. If the qualifier is "=", the IC50 result equals the value in that column; if the qualifier is ">", the IC50 result is greater than that value.String
476IC50_1_Range6IC50 value determined using sigmoidal dose response equationFloatμM
477Std.Err(IC50)_1_Range6Standard Error of IC50 valueFloatμM
478nH_1_Range6Hill coefficient determined using sigmoidal dose response equationFloat
479Excluded_Points_first_point_Range6Flags to indicate which of the first dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
480% inhibition at 0.5 uM_first_point_Range6 (0.5μM**)% inhibition at a given concentrationFloat%
481% inhibition at 0.25 uM_first_point_Range6 (0.25μM**)% inhibition at a given concentrationFloat%
482% inhibition at 0.125 uM_first_point_Range6 (0.125μM**)% inhibition at a given concentrationFloat%
483% inhibition at 0.0625 uM_first_point_Range6 (0.0625μM**)% inhibition at a given concentrationFloat%
484% inhibition at 0.03125 uM_first_point_Range6 (0.03125μM**)% inhibition at a given concentrationFloat%
485% inhibition at 0.015625 uM_first_point_Range6 (0.015625μM**)% inhibition at a given concentrationFloat%
486% inhibition at 0.0078125 uM_first_point_Range6 (0.0078125μM**)% inhibition at a given concentrationFloat%
487% inhibition at 0.00390625 uM_first_point_Range6 (0.00390625μM**)% inhibition at a given concentrationFloat%
488% inhibition at 0.001953125 uM_first_point_Range6 (0.00195312μM**)% inhibition at a given concentrationFloat%
489% inhibition at 0.0009765625 uM_first_point_Range6 (0.000976562μM**)% inhibition at a given concentrationFloat%
490Excluded_Points_second_point_Range6Flags to indicate which of the second dose-response points were excluded from analysis. (1) means the titration point was (1) excluded and (0) means the point was not excluded, for the titration series going from low to high compound concentrations.String
491% inhibition at 0.5 uM_second_point_Range6 (0.5μM**)% inhibition at a given concentrationFloat%
492% inhibition at 0.25 uM_second_point_Range6 (0.25μM**)% inhibition at a given concentrationFloat%
493% inhibition at 0.125 uM_second_point_Range6 (0.125μM**)% inhibition at a given concentrationFloat%
494% inhibition at 0.0625 uM_second_point_Range6 (0.0625μM**)% inhibition at a given concentrationFloat%
495% inhibition at 0.03125 uM_second_point_Range6 (0.03125μM**)% inhibition at a given concentrationFloat%
496% inhibition at 0.015625 uM_second_point_Range6 (0.015625μM**)% inhibition at a given concentrationFloat%
497% inhibition at 0.0078125 uM_second_point_Range6 (0.0078125μM**)% inhibition at a given concentrationFloat%
498% inhibition at 0.00390625 uM_second_point_Range6 (0.00390625μM**)% inhibition at a given concentrationFloat%
499% inhibition at 0.001953125 uM_second_point_Range6 (0.00195312μM**)% inhibition at a given concentrationFloat%
500% inhibition at 0.0009765625 uM_second_point_Range6 (0.000976562μM**)% inhibition at a given concentrationFloat%

* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: MH077602-01

Data Table (Concise)
Data Table ( Complete ):     View Active Data    View All Data
Classification
PageFrom: