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BioAssay: AID 1

NCI human tumor cell line growth inhibition assay. Data for the NCI-H23 Non-Small Cell Lung cell line

Growth inhibition of the NCI-H23 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the more ..
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 Tested Compounds
 Tested Compounds
All(40989)
 
 
Active(2104)
 
 
Inactive(38796)
 
 
Unspecified(103)
 
 
 Tested Substances
 Tested Substances
All(42490)
 
 
Active(2300)
 
 
Inactive(40085)
 
 
Unspecified(105)
 
 
 Related BioAssays
 Related BioAssays
AID: 1
Data Source: DTP/NCI
BioAssay Type: Confirmatory, Concentration-Response Relationship Observed
Deposit Date: 2004-08-15
Modify Date: 2005-08-10

Data Table ( Complete ):           Active    All
BioActive Compounds: 2104
Description:
Growth inhibition of the NCI-H23 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number. These estimates are done by simple linear interpolation between the concentrations that surround the approriate level. If a compound doesn't cause inhibition to the appropriate level, the endpoint is set to the highest concentration tested.
Comment
These data are a subset of the data from the NCI human tumor cell line screen. Compounds are identified by the NCI NSC number. In the NCI numbering system, NCI-H23 is panel number 1, cell number 1
Basically compounds with LogGI50 (unit M) less than -6 were considered as active. Activity score was based on increasing values of -LogGI50.
Result Definitions
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TIDNameDescriptionHistogramTypeUnit
OutcomeThe BioAssay activity outcomeOutcome
ScoreThe BioAssay activity ranking scoreInteger
1loggi50Log of the GI50 result, unit: M.FloatM
2loggi50Log of the GI50 result, unit: ug/mL.Floatug/mL
3loggi50Log of the GI50 result, unit: Volumetric.Floatv
4indngi50Number of tests averaged for the GI50 value for this NSC and cell line.Integer
5stddevgi50Standard Deviation of the Log10 of the GI50 result averaged across all tests for this NSC and cell line.Float
6logtgiLog of the TGI result, unit: M.FloatM
7logtgiLog of the TGI result, unit: ug/mL.Floatug/mL
8logtgiLog of the TGI result, unit: Volumetric.Floatv
9indntgiNumber of tests averaged for the TGI value for this NSC and cell line.Integer
10stddevtgiStandard Deviation of the Log10 of the TGI result averaged across all tests for this NSC and cell line.Float

Data Table (Concise)
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